scholarly journals Actividad antioxidante de extractos de hojas de Bocconia frutescens L. (Papaveraceae)

2016 ◽  
Vol 14 (2) ◽  
Author(s):  
Oscar Eduardo Rodríguez Aguirre ◽  
William Alejandro Andrade Barreiro ◽  
William Alejandro Andrade Barreiro ◽  
Fabio Eduardo Diaz Lopez ◽  
Fabio Eduardo Diaz Lopez
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Dpph Radical ◽  
Small Tree ◽  
High Antioxidant Activity ◽  
Para Determinar ◽  
Percent Uptake ◽  
Orange Color ◽  
Higher Sensitivity

<p>B<em>occonia frutescens </em>es un árbol pequeño, posee hojas profundamente lobuladas y con borde dentado, las flores están agrupadas en panículas terminales pendulares, las semillas son de color negro brillante con una cubierta carnosa de color anaranjada. Es nativa de América, es conocida con los nombres vulgares de: Sarno, Palo Amarillo o Trompeto. <strong>Objetivos: </strong>Evaluar la actividad antioxidante de extractos y fracciones de hojas de Bocconia frutescens por los métodos DPPH• y ABTS•+. <strong>Métodos: </strong>las fracciones obtenidas por soxleth con solventes de diferente polaridad de hojas, fueron evaluadas a concentraciones de 25, 62.5, 125 y 250 mg/LMeOH para determinar la actividad antioxidante por los métodos DPPH• y ABTS•+. <strong>Resultados: </strong>Para los extractos por el método decoloración del radical DPPH• el Porcentaje de Captación se encontró entre 47.6 y 57.7 a 250 mg/LMeOH y para las fracciones entre 45.4 y 54.1 mg/LMeOH, a 250 mg/LMeOH. Por el método decoloración del radical ABTS•+. Para los extractos el porcentaje de Captación se encontro entre 89.7 y 99.7 a 250 mg/LMeOH y para las fracciones entre 68.5 y 99.6 mg/LMeOH. <strong>Conclusiones: </strong>las fracciones de acetato de etilo y metanol presentaron una alta actividad antioxidante al ser evaluadas por las técnicas ABTS•+ y DPPH• , la técnica ABTS•+ se presento mayor sensibilidad.</p><p>Abstract</p><p>Bocconia frutescens is a small tree, has deeply lobed and serrated edge leaves, flowers are grouped in pendulous panicles terminal, seeds are shiny black with a fleshy covering of orange color. It is native to America, is known under their common names: Sarno, Palo Amarillo or Trompeto. Objectives: Evaluate the antioxidant activity of extracts and fractions of leaves Bocconia frutescens by DPPH• and ABTS•+ methods. Methods: the fractions obtained by Soxhlet with solvents of different polarity of leaves, were evaluated at concentrations of 25, 62.5, 125 and 250 mg / LMeOH to determine the antioxidant activity by DPPH• and ABTS•+ methods. Results: Extracts for the bleaching method DPPH• radical the Percent Uptake was between 47.6 and 57.7 to 250 mg / LMeOH and fractions between 45.4 and 54.1 mg / LMeOH, to 250 mg / LMeOH. By the method of coloration radical ABTS•+. Extracts for the percentage uptake was found between 89.7 and 99.7 and fractions between 68.5 and 99.6 mg / LMeOH, to 250 mg / LMeOH. Conclusions: The fractions of ethyl acetate and methanol have a high antioxidant activity to be evaluated by the ABTS•+ and DPPH• techniques, the technique ABTS•+ present higher sensitivity.</p>

scholarly journals Quick Preparative Separation of Natural Naphthopyranones with Antioxidant Activity by High-Speed Counter-Current Chromatography

2002 ◽  
Vol 57 (11-12) ◽  
pp. 1051-1055 ◽  
Author(s):  
Gilda G. Leitão ◽  
Suzana G. Leitão ◽  
Wagner Vilegas
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
High Speed ◽  
Ethanolic Extract ◽  
Solvent System ◽  
Spectrophotometric Assay ◽  
Preparative Separation ◽  
Dpph Radical ◽  
Preparative Scale ◽  
Counter Current

The natural naphthopyranones paepalantine (1), paepalantine-9O-β-ᴅ-glucopyranoside (2) and paepalantine-9-O-β-ᴅ-allopyranosyl-(1→6)-O-β-ᴅ-glucopyranoside (3) were separated in a preparative scale from the ethanolic extract of the capitula of Paepalanthus bromelioides by high-speed counter-current chromatography (HSCCC). The solvent system used was composed of water-ethanol-ethyl acetate-hexane (10:4 : 10:4, v/v/v/v). This technique led to the separation of the three different naphthopyranone glycosides in pure form in approximately 7 hours. Paepalantine showed a good antioxidant activity when assayed by the DPPH radical spectrophotometric assay.

scholarly journals ANTIOXIDANT ACTIVITIES OF FRACTIONS FROM ETHYL ACETATE EXTRACTS OF GARCINIA FRUTICOSA LAUTERB. LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 44 ◽  
Author(s):  
Riza Shabrina ◽  
Berna Elya ◽  
Arikadia Noviani
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Group Identification ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Dpph Radical Scavenging

Objective: This study aimed to fractionate the antioxidant activity of the ethyl acetate leaf extract and to characterize the most active fractionsaccording to compound groups.Methods: The ethyl acetate extract was fractionated with column chromatography using a gradient elution system. Fractions were first screenedqualitatively for antioxidant activity before active fractions were quantified with respect to in vitro antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the ferric-reducing antioxidant power (FRAP) assay. The compound groups were identifiedfollowing separation by thin-layer chromatography.Results: Fraction 11 exhibited the greatest DPPH radical-scavenging activity, with an IC50 value of 6.58 μg/mL, while the fraction with the greatestantioxidant activity according to the FRAP assay was fraction 10, with a ferric ion equivalent antioxidant activity value of 1015.34 μmol/g.Conclusion: Compound group identification revealed that Fractions 10 and 11 contained flavonoids, with two common to both fractions, whilefraction 10 also contained one specific flavonoid.

scholarly journals Phytochemical screening, free radical scavenging, antioxidant activity and phenolic content of Dodonaea viscosa

2012 ◽  
Vol 77 (4) ◽  
pp. 423-435 ◽  
Author(s):  
Tauheeda Riaz ◽  
Athar Abbasi ◽  
A Aziz-Ur-Rehman ◽  
Tayyaba Shahzadi ◽  
Muhammad Ajaib ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Antioxidant Activity ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dodonaea Viscosa ◽  
Total Antioxidant

The purpose of this study was to evaluate the antioxidant potential of Dodonaea viscosa Jacq. Methanolic extract of the plant was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and nbutanol sequentially. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol fraction. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant activity, Ferric Reducing Antioxidant Power (FRAP) assay and ferric thiocyanate assay along with determination of their total phenolics. The results revealed that ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 81.14 ? 1.38% inhibition of DPPH radical at a concentration of 60 ?g/ml. The IC50 of this fraction was found to be 33.95 ? 0.58 ?g/ml, relative to butylated hydroxytoluene (BHT), having IC50 of 12.54 ? 0.89 ?g/mL. It also showed highest FRAP value (380.53 ? 0.74 ?M of trolox equivalents) as well as highest total phenolic contents (208.58 ? 1.83 GAE ?g/g) and highest value of inhibition of lipid peroxidation (58.11 ? 1.49% at concentration of 500 ?g/ml) as compared to the other studied fractions. The chloroform fraction showed highest total antioxidant activity i.e.1.078 ? 0.59 (eq. to BHT).

scholarly journals Aktivitas Antioksidan Fenolik Total Ekstrak Kulit Biji Jengkol (Archidendron Jiringa (Jack) I. C. Nielsen)

2019 ◽  
Vol 2 (1) ◽  
pp. 72-78
Author(s):  
Misri Yanty Lubis ◽  
Lamek Marpaung ◽  
Muhammad Pandapotan Nasution ◽  
Partomuan Simanjuntak
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Phenolic Antioxidant ◽  
Total Phenolic ◽  
Skin Extract ◽  
Dpph Method ◽  
High Antioxidant Activity ◽  
X 24

Penelitian ini dilakukan untuk menentukan aktivitas antioksidan fenolik total dari ekstrak kulit biji jengkol. Kulit biji jengkol dalam bentuk serbuk yang sudah dikering angin anginkan selama 1x24 jam dimaserasi dengan metanol selama 1x24 jam. Ekstrak yang diperoleh dilarutkan dengan air secara berulang-ulang dan kemudian fraksi air dipartisi dengan etil asetat berulang-ulang. Selanjutnya ekstrak pekat dilarutkan dengan metanol dan diparitsi dengan n-heksan untuk memperoleh fenolik total. Metode DPPH digunakan untuk menentukan aktivitas antioksidan. Nilai IC 50 yang diperoleh adalah 11,7987. Nilai ini menunjukkan aktivitas antioksidan yang cukup tinggi.   This study was conducted to determine the total phenolic antioxidant activity of jengkol seed skin extract. Jengkol seed slayer in the form of powder that had been dried for 1 x 24 hours macerated with methanol for 1 x 24 hours. The extract was dissolved with water repeatedly and then the fraction of water was partitioned with ethyl acetate repeatedly. Then the concentrated extract was dissolved with methanol and paritized with n-hexane to obtain the total phenolic. The DPPH method was used to determine antioxidant activity. The IC 50 value obtained was 11.7987. This value showed quite high antioxidant activity.

scholarly journals COMPARATIVE ANALYSIS OF PHYTOCHEMICAL, ANTIBACTERIAL, AND ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS OF AZADIRACHTA INDICA LEAVES

2019 ◽  
pp. 151-154
Author(s):  
GAURAV SHARMA ◽  
ANKITA THAKUR ◽  
SOHAN LAL ◽  
ROHIT KUMAR NADDA
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Azadirachta Indica ◽  
Comparative Evaluation ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Bacterial Strains ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

Objective: The objective of the present study was the analysis of phytochemicals in various extracts of Azadirachta indica leaves, comparative evaluation of antibacterial activity of the various extracts of A. indica leaves against Escherichia coli and Staphylococcus aureus, and comparative evaluation of antioxidant activity in various extracts of A. indica leaves using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Methods: Various extracts were prepared by crushing the samples. Antibacterial susceptibility test, various phytochemical tests for qualitative analysis, and DPPH radical scavenging assay for antioxidant activity were performed. Results: The result suggested that alkaloids, flavonoids, and terpenoids were present in all the four extracts. Tannins were absent in the ethyl acetate extract, and phenols were only present in the ethyl acetate extract. Sterols and phlobatannins were absent in all the four extracts. Saponins were only present in the aqueous extract, and amino acids were only present in the ethyl acetate extract. The bacterial strains S. aureus and E. coli were used against the different extracts of A. indica leaves, i.e., methanol, chloroform, ethyl acetate, and aqueous. Conclusion: The results suggested that bioactive compounds found in leaves of A. indica contribute to its pharmacological activities.

scholarly journals Antioxidant activities of Dichrostachys cinerea (L.) Wight et Arn (Leguminosae): Correlations between the Polyphenol Level and the Antioxidant Activity

2020 ◽  
pp. 1-11
Author(s):  
Reine Raïssa Rolande Aworet Samseny ◽  
Line-Edwige Mengome ◽  
Sophie Aboughe Angone
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Antiradical Activity ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Dpph Radical ◽  
Dichrostachys Cinerea ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Aims: Free radicals are involved in many diseases. Antioxidants help control them. The overproduction of free radicals or reduction of natural antioxidants promotes the destruction of cells in our body, for example in neurodegenerative diseases. The aim of our study is to evaluate the correlation between the level of polyphenol and antioxidant activity of Dichrostachys cinerea barks he work was carried out at the phytochemistry department of the Institute of Pharmacopoeia and Traditional Medicine (Gabon). Methodology: Different extracts of Dichrostachys cinerea barks were obtained with polarity solvent (dichloromethane, ethyl acetate, butanol, methanol and water).  The antioxidant activity is evaluating using respectively FRAP method (Ferric Reducing Antioxidant Power), antiradical activity by the method of inhibition of DPPH radical (2.2-diphenyl-1-picrylhydrazyl), cations ABTS radical (2.2'-azinobis-[3-acid-6-sulfonic ethylenzothiazoline]). Results: Ethyl acetate and dichloromethane extract have the higher value for polyphenolic compounds, respectively 52,27±0,66 mg EAG / g and 49,72±0,55 mg EAG / g. Aqueous extract have the lower value 20,67±1,05 mg EAG / g. The different fractions of Dichrostachys cinerea have antioxidant effect, and this effect is in correlation with its constituents: polyphenols, tannins and flavonoids. Results were compared to standard antioxidants such as ascorbic acid and quercetin. Conclusion: This study confirms higher is the level of polyphenols greater is the antioxidant power.

Relative Antioxidant Activity of Common Indian Herbs

2011 ◽  
Vol 7 (1) ◽  
Author(s):  
Padma S Vankar ◽  
Jyoti Srivastava
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Ash Content ◽  
Total Phenol ◽  
Dpph Radical ◽  
Medicinal Purpose ◽  
Frap Assay ◽  
Plant Materials ◽  
High Antioxidant Activity ◽  
Very High

Ten Indian herbs (Manjisth, Tagar, Kulfa, Khuskhus, Gokharu, Carrot, Kasni, Bathua, Soya ke Beej, Pudina), popularly used for medicinal purpose, were extracted in sonicator by methanol and water for the estimation of polyphenols and flavonoids. Antioxidant capacities (AOC) of extracts were evaluated based on their ability to scavenge the DPPH radical and FRAP Assay. The results showed that methanolic extract of all the plant materials had very high antioxidant activity. Amongst them, Pudina extract in the methanol showed highest value of AOC by both FRAP and DPPH methods (33 percent and 1.36 mg QEq / 100 g). Total phenol and flavonoids content were also evaluated for these extracts. It varied from 0.8736 to 6.3918 mg GAE / 100 g and 0.3642 to 1.9876 mg QE / 100 g. Total ash content and acid insoluble content of soya showed highest value i.e. 14.55 percent and 92.92 percent.

Isolation and Structure Determination of Antioxidants Active Compounds from Ethyl Acetate Extract of Heartwood Namnam (Cynometra cauliflora L.)

2017 ◽  
Vol 19 (1) ◽  
pp. 11-17
Author(s):  
Dede Sukandar ◽  
Siti Nurbayti ◽  
Tarso Rudiana ◽  
Tsalats Wahyul Husna
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Aromatic Ring ◽  
1H Nmr ◽  
Ethyl Acetate Extract ◽  
Active Compounds ◽  
Chromophore Group ◽  
Ic50 Value ◽  
High Antioxidant Activity

Active compounds with antioxidant activity were isolated from ethyl acetate extract of namnam stem (C. cauliflora L.) that had undergone maceration and fractionation by gravity column chromatography. The compounds were later identified by by using UV-Vis Spectrophotometry, FTIR, LCMS and 1H-NMR. Ethyl acetate extract of namnam stem showed considerably high antioxidant activity (IC50 value 4.68 ± 0.035 ppm). The results of analysis by UV-Vis and FTIR showed carbonyl group conjugated with an aromatic ring at band I (λmax 330.22 nm), chromophore group of alkene (C=C) at band II (λmax 268.67 nm) and functional groups such as O−H (3343.91 cm-1), C=O (1729.23 cm-1), C=C (1652.64 and 1611.99 cm-1), C−O (1269.89) and C−H ortho (738.23 cm-1). LCMS (m/z 270.9246) and 1H-NMR data showed seven proton signals on the aromatic ring at carbon position C-3 at δH 6.86 ppm (1H, s), C-6 at δH 5.95 ppm (1H, d, J=1.95 Hz), C-8 at δH 6.25 ppm (1H, d, J= 1.95 Hz), C-2’ and C-6’ at δH 7.03 ppm (2H, d, J=7.87 Hz), C-3’ and C-5’ at δH 6.87 ppm (2H, d, J= 7.87 Hz) so that the structure was identified as a flavonoid which was 4 ', 5,7-trihydroxy-flavones or known as apigenin. The isolated apigenin had very strong antioxidant activity, as shown by IC50 value of 5.18 ± 0.014 ppm.

scholarly journals EVALUATION AND OPTIMIZATION OF ANTIOXIDANT POTENTIALITY OF XYLARIA FEEJEENSIS HMJAU22039

2016 ◽  
pp. 269 ◽  
Author(s):  
Durga Pallavi Rebbapragada ◽  
Rajagopal Kalyanaraman
Keyword(s):  
Antioxidant Activity ◽  
Yeast Extract ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Basal Medium ◽  
Tectona Grandis ◽  
Total Phenolic ◽  
Dpph Radical ◽  
High Antioxidant Activity ◽  
Dpph Radical Scavenging

<p>ABSTRACT<br />Objective: Antioxidants neutralize free radicals generated in the human body and prevent them from causing damage. The present study is aimed to<br />evaluate and optimize the antioxidant activity of Xylaria feejeensis HMJAU22039 an endophyte isolated from Tectona grandis.<br />Methods: The antioxidant potential was measured by 1-1diphenyl-2-picryl-hydrazil (DPPH) radical scavenging assay and nitric oxide (NO) scavenging<br />assay. Total phenolic content (TPC) of the samples was measured by Folin–Ciocalteu reagent based assay, and the values were obtained from the<br />regression equation: y = 0.006141x + 0.157733.<br />Results: Potato dextrose yeast extract broth medium was selected as the basal medium as it reported a high antioxidant activity. The basal medium<br />was optimized with 2% dextrose, 0.2% yeast extract, and 200 g/L potato infusion. Dextrose and yeast extract were selected as carbon and nitrogen<br />sources, respectively, as they reported high antioxidant activity. The antioxidant activity and phenolic content are highest at static condition<br />(18.14 mg/g). Incubation temperature of 30°C and pH of the initial medium at 6 were found to be optimum conditions for high antioxidant activity. An<br />incubation period of 20 days reported the highest antioxidant activity and phenolic content. Methanol extract recorded high antioxidant activity with<br />a DPPH (73.86%) followed by ethyl acetate and chloroform fractions.<br />Conclusion: The study highlights the importance of different physiochemical parameters in the production of secondary metabolites having<br />antioxidant properties. The results reveal a significant positive correlation between DPPH radical scavenging assay, NO scavenging assay, and TPC.<br />Keywords: 1-1diphenyl-2-picryl-hydrazil, Total phenolic content, Endophytic fungus, Tectona grandis.</p>

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