Identifying Snow Leopards, Tigers and other Endangered Species by developing a low-cost DNA field test kit - Part 2

10.18258/9670 ◽  
2017 ◽  
Author(s):  
Natalie Schmitt Natalie Schmitt
Keyword(s):  
Endangered Species ◽  
Field Test ◽  
Low Cost ◽  
Test Kit ◽  
Snow Leopards

scholarly journals Evaluation of a rapid diagnostic field test kit for identification of Phytophthora species, including P. ramorum and P. kernoviae at the point of inspection

2007 ◽  
Vol 56 (5) ◽  
pp. 828-835 ◽  
Author(s):  
C. R. Lane ◽  
E. Hobden ◽  
L. Walker ◽  
V. C. Barton ◽  
A. J. Inman ◽  
...  
Keyword(s):  
Field Test ◽  
Phytophthora Species ◽  
Test Kit

Evaluation of the Hanby Field Test Kit for Detection of Aromatics in Water

2009 ◽  
pp. 282-282-7
Author(s):  
IAL Rhodes ◽  
RW Claybon ◽  
HL Wisniewski ◽  
AL Calkin ◽  
JA Leon ◽  
...  
Keyword(s):  
Field Test ◽  
Test Kit

scholarly journals A Thin Layer of Activated Carbon Deposited on Polyurethane Cube Leads to New Conductive Bioanode for (Plant) Microbial Fuel Cell

Energies ◽  
2020 ◽  
Vol 13 (3) ◽  
pp. 574
Author(s):  
Emilius Sudirjo ◽  
Paola Y. Constantino Diaz ◽  
Matteo Cociancich ◽  
Rens Lisman ◽  
Christian Snik ◽  
...  
Keyword(s):  
Fuel Cells ◽  
Activated Carbon ◽  
Field Test ◽  
Microbial Fuel Cells ◽  
Large Scale ◽  
Electrode Materials ◽  
Low Cost ◽  
Three Dimensional ◽  
Polyurethane Foams ◽  
Electrochemically Active

Large-scale implementation of (plant) microbial fuel cells is greatly limited by high electrode costs. In this work, the potential of exploiting electrochemically active self-assembled biofilms in fabricating three-dimensional bioelectrodes for (plant) microbial fuel cells with minimum use of electrode materials was studied. Three-dimensional robust bioanodes were successfully developed with inexpensive polyurethane foams (PU) and activated carbon (AC). The PU/AC electrode bases were fabricated via a water-based sorption of AC particles on the surface of the PU cubes. The electrical current was enhanced by growth of bacteria on the PU/AC bioanode while sole current collectors produced minor current. Growth and electrochemical activity of the biofilm were shown with SEM imaging and DNA sequencing of the microbial community. The electric conductivity of the PU/AC electrode enhanced over time during bioanode development. The maximum current and power density of an acetate fed MFC reached 3 mA·m−2 projected surface area of anode compartment and 22 mW·m−3 anode compartment. The field test of the Plant-MFC reached a maximum performance of 0.9 mW·m−2 plant growth area (PGA) at a current density of 5.6 mA·m−2 PGA. A paddy field test showed that the PU/AC electrode was suitable as an anode material in combination with a graphite felt cathode. Finally, this study offers insights on the role of electrochemically active biofilms as natural enhancers of the conductivity of electrodes and as transformers of inert low-cost electrode materials into living electron acceptors.

scholarly journals Evaluation of an Immunochromatographic Assay as a Canine Rabies Surveillance Tool in Goa, India

Viruses ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 649 ◽  
Author(s):  
Yale ◽  
Gibson ◽  
Mani ◽  
P.K. ◽  
Costa ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Low Cost ◽  
Antibody Test ◽  
Immunochromatographic Assay ◽  
Human Rabies ◽  
Post Mortem ◽  
Laboratory Equipment ◽  
Tissue Samples ◽  
Test Kit ◽  
The Government

Rabies is a fatal zoonotic disease transmitted by the bite of a rabid animal. More than 95% of the human rabies cases in India are attributed to exposure to rabid dogs. This study evaluated the utility of a lateral flow immunochromatographic assay (LFA) (Anigen Rapid Rabies Ag Test Kit, Bionote, Hwaseong-si, Korea) for rapid post mortem diagnosis of rabies in dogs. Brain tissue was collected from 202 animals that were screened through the Government of Goa rabies surveillance system. The brain tissue samples were obtained from 188 dogs, nine cats, three bovines, one jackal and one monkey. In addition, 10 dogs that died due to trauma from road accidents were included as negative controls for the study. The diagnostic performance of LFA was evaluated using results from direct fluorescence antibody test (dFT); the current gold standard post mortem test for rabies infection. Three samples were removed from the analysis as they were autolysed and not fit for testing by dFT. Of the 209 samples tested, 117 tested positive by LFA and 92 tested negative, while 121 tested positive by dFT and 88 tested negative. Estimates of LFA sensitivity and specificity were 0.96 (95% CI 0.91–0.99) and 0.99 (95% CI 0.94–1.00), respectively. The LFA is a simple and low-cost assay that aids in the rapid diagnosis of rabies in the field without the need for expensive laboratory equipment or technical expertise. This study found that Bionote LFA has potential as a screening tool in rabies endemic countries.

Development of Field Test Kits for Determination of Microencapsulated Iron in Double-Fortified Salt

2008 ◽  
Vol 29 (4) ◽  
pp. 288-296 ◽  
Author(s):  
Jessica S. Yuan ◽  
Yao O. Li ◽  
Judy W. Ue ◽  
Annie S. Wesley ◽  
Levente L. Diosady
Keyword(s):  
Field Test ◽  
Analytical Methods ◽  
Field Tests ◽  
Incidence Rates ◽  
Deficiency Anemia ◽  
Colorimetric Determination ◽  
Iron Compounds ◽  
Iron Determination ◽  
Detection Range ◽  
Test Kit

Background Efficacy studies have shown that salt double-fortified with iodine and iron can significantly reduce the incidence rates of iron-deficiency anemia and iodine-deficiency disorders. Double-fortified salt can be prepared by mixing microencapsulated iron compounds into conventionally iodated salt. Effective implementation of a double fortification program requires field-based analytical methods to ensure iron levels in double-fortified salt. Objective To develop semiquantitative and qualitative field test kits by adopting standard analytical methods for iron determination to the analysis of iron in double-fortified salt. Methods Thermal, mechanical, and chemical strategies were assessed to enable contact between analytical reagents and the encapsulated iron compounds during the analysis. A chemical approach using nonpolar solvents was adopted in semiquantitative and qualitative field tests. The fat coating of the iron premix was removed by solvents, releasing the iron for subsequent colorimetric determination. Results Both semiquantitative and qualitative field tests were based on initial removal of the microencapsulant, followed by iron quantitation. Solvent dissolution of the coating layer was most useful for rapid release of iron. A semiquantitative field test kit was developed using a mixture of 5% heptane and 95% tetrachloroethylene to free the iron, which was then determined by the 1,10-phenanthroline method. The field test had a useful detection range of 0 to 2,000 ppm of iron. Statistical analyses revealed that the results obtained with the kit correlated well with those obtained by standard laboratory methods ( p < .001). A qualitative field test kit was developed to identify the presence of iron. Microencapsulated iron was freed with the use of tetrachloroethylene and then reacted with phenanthroline to form a visually observable coloration on the salt sample. Conclusion Semiquantitative and qualitative field test kits for iron determination in double-fortified salt have been developed and tested. These kits could be useful in quality control of double fortification of salt in small salt-production facilities and in the field, particularly in developing countries.

Evaluation of a Field Test Kit for Monitoring Lead in Drinking Water

1993 ◽  
Vol 85 (8) ◽  
pp. 90-100 ◽  
Author(s):  
Michael R. Schock ◽  
Gregory K. George
Keyword(s):  
Drinking Water ◽  
Field Test ◽  
Test Kit

scholarly journals Design of a low-cost measuring and plotting device for photovoltaic modules

2019 ◽  
Vol 52 (9-10) ◽  
pp. 1308-1318
Author(s):  
Sudipta Basu Pal ◽  
Abhijit Das ◽  
Konika Das (Bhattacharya) ◽  
Dipankar Mukherjee
Keyword(s):  
Heat Dissipation ◽  
Low Cost ◽  
Climatic Conditions ◽  
Performance Ratio ◽  
Photovoltaic Module ◽  
Eastern Region ◽  
Testing Apparatus ◽  
Photovoltaic Modules ◽  
Test Kit ◽  
Capacitive Loading

The photovoltaic module testing apparatus being used presently for photovoltaic measurements acts principally on the method of photovoltaic module loading with resistive, capacitive, and electronic elements. In this work, a new method is described using a supercapacitor as the load to the photovoltaic module. This technique of characterization has proved to generate reliable V–I characteristics as validated by statistical and mathematical analyses in this article. Heat dissipation affecting the functioning of the photovoltaic modules is a common occurrence with resistive and capacitive loading techniques. It is reduced significantly in this method using supercapacitors, and curve tracing time is extremely modest and easily controllable. In effect, a low-cost, portable, and reliable I–V plotter is developed, which is operational from an embedded systems platform integrated with smart sensors. This I–V tracer has been used for the performance assessment of solar modules ranging from 10 to 100 Wp under varying climatic conditions in the eastern region of India. This test kit so developed in the photovoltaic engineering laboratory at Indian Institute of Engineering Science and Technology, Shibpur, is estimated to be useful for practicing engineers and photovoltaic scientists and in particular for photovoltaic module manufacturers. The performance parameters such as fill factor and performance ratio of photovoltaic modules measured by the device have been found to have almost identical values as the measurements from a reference commercial testing apparatus. The data pertaining to peak wattage as measured by the designed plotter have been found to be closely converging with an industry-friendly YOKOGAWA Power Meter (WT 330). Such peak values of power as measured and claimed by the datasheets will help reduce the uncertainties in measurement, leading to increased confidence of photovoltaic module manufacturers and investors. With this backdrop, the necessary work for scaling up of the low-cost I–V plotter has been taken up for assessing the performance of higher wattage photovoltaic modules.

scholarly journals Letter: Limited accuracy of arsenic field test kit

2004 ◽  
Vol 38 (24) ◽  
pp. 479A-479A ◽  
Author(s):  
Nadim Reza Khandaker
Keyword(s):  
Field Test ◽  
Test Kit ◽  
Limited Accuracy
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