scholarly journals Shellfish Allergy: Unmet Needs in Diagnosis and Treatment

2020 ◽  
Vol 30 (6) ◽  
pp. 409-420 ◽  
Author(s):  
S Gelis ◽  
M Rueda ◽  
A Valero ◽  
EA Fernández ◽  
M Moran ◽  
...  
Keyword(s):  
Food Allergy ◽  
Calcium Binding ◽  
Myosin Light Chain ◽  
Arginine Kinase ◽  
Published Data ◽  
Allergic Reactions ◽  
Food Groups ◽  
Allergenic Proteins ◽  
Shellfish Allergy ◽  
Big Eight

Seafood is a major cause of food allergy and anaphylaxis worldwide. Shellfish is included among the “big eight” food groups, which are responsible for more than 90% of all cases of food allergy. Approximately 2.5% of the world’s population has experienced an adverse reaction to seafood. Seafood allergy is one of the most frequent and lethal allergies that exist. The several allergenic proteins involved in allergic reactions that have been described in recent years include tropomyosin, arginine kinase, myosin light chain, and sarcoplasmic calcium-binding protein. Despite all the data reported in the last few years, shellfish allergy is still diagnosed and treated as it was 50 years ago. The only effective treatment to prevent allergic reactions to shellfish is avoidance. This review aims to update recently published data on shellfish allergy and to highlight those areas that have yet to be resolved.

A Review on Identified Major Food Allergens: Characteristics and Role in Food Allergy

2017 ◽  
Vol 54 (3) ◽  
pp. 346
Author(s):  
Chhavi Arya ◽  
Chetna Jantwal
Keyword(s):  
Food Allergy ◽  
Food Allergies ◽  
Allergic Reactions ◽  
Food Allergens ◽  
Food Groups ◽  
Consumer Protection Act ◽  
Major Food ◽  
Life Threatening ◽  
Major Allergens ◽  
Allergen Labeling

Food allergens are the substances present in food that cause food allergy. Human body reactions to food allergens range from mild to severe life threatening anaphylactic shock. At least seventy different foods have been reported to cause allergic reactions and several other foods have been identified which have the potential to provoke allergic reactions. Majority of the identified food allergens are proteins. The Food Allergen Labeling and Consumer Protection Act (FALCPA) identifies eight major food groups i.e. milk, eggs, fish, crustacean shellfish, tree nuts, peanuts, wheat, and soybeans as major allergy causing foods. These eight foods are believed to account for 90 per cent of food allergies and are responsible for most serious reactions to foods. Several studies have been done which identify the major allergens in various foods. The present paper attempts to review the major allergens present in various food.

scholarly journals CLINICAL PROPERTIES OF FOOD ALLERGENS

2017 ◽  
Vol 14 (6) ◽  
pp. 59-68
Author(s):  
O S Fedorova ◽  
M M Fedotova ◽  
L M Ogorodova ◽  
E M Kamaltynova ◽  
T A Nagaeva
Keyword(s):  
Food Allergy ◽  
Food Source ◽  
Clinical Manifestations ◽  
Medical Community ◽  
Allergic Reactions ◽  
Food Allergens ◽  
Significant Progress ◽  
Therapeutic Approaches ◽  
Molecular Allergology ◽  
Allergenic Proteins

Food allergy is the problems of great interest in medical community. Usually food allergy starts in infants and it is one of the main reasons of severe allergic reactions, including death as a result of anaphylaxis. In the last two decades, the development of molecular allergology facilitated significant progress in understanding of basis of food allergy. Researches in field of molecular allergology revealed that a food source can contain several different proteins responsible for allergic reaction development. Molecular structure and stability of epitopes of allergenic proteins can lead to sensitization development and severity of clinical manifestations. The aim of the present paper is to review the latest data on molecular allergology in the field of perspective diagnostics and therapeutic approaches to food allergy.

Clinical and immunological characteristics of sensitization to tropomyosins

2021 ◽  
Vol 18 (1) ◽  
pp. 73-78
Author(s):  
Marina A. Mokronosova ◽  
Tatiana M. Zheltikova
Keyword(s):  
Food Allergy ◽  
Allergic Reaction ◽  
Clinical Case ◽  
Oral Allergy Syndrome ◽  
Allergic Reactions ◽  
Allergenic Proteins ◽  
Primary Sensitization ◽  
Life Threatening ◽  
Reaction Severity ◽  
High Concentrations

Tropomyosins are a family of allergenic proteins found in large quantities in all invertebrates. Tropomyosins sensitization causes a life-threatening allergic reaction up to anaphylaxis after eating seafood. Identifying the source of primary sensitization is important to predict the allergic reaction severity. This article describes a clinical case of chronic recurrent urticaria in an 8-year-old boy with tropomyosins sensitization. An 8-year-old boy was diagnosed with the following: controlled atopic phenotype bronchial asthma, food allergy (oral allergy syndrome), and chronic recurrent spontaneous urticaria. Component diagnostics revealed IgE-aB to tropomyosins in high concentrations from 38.79 to 43.38 kUA/l and cat and dog uteroglobin and lipocalins in high concentrations from 7.79 to 43.38 kUA/l. It is necessary to specify the primary sensitizer to analyze the clinical significance of allergens that provoke sensitization to various groups of allergens. In this case, sensitization to tropomyosins is most likely described as caused by either a helminthic invasion or midge bites. Therefore, food allergic reactions to tropomyosins caused from crustaceans were not observed.

Sequence homology of the fly proteins tropomyosin, arginine kinase and myosin light chain with known allergens in invertebrates

2016 ◽  
Vol 2 (2) ◽  
pp. 69-81 ◽  
Author(s):  
M.R. Romero ◽  
A.J. Claydon ◽  
E.C. Fitches ◽  
M.E. Wakefield ◽  
A.J. Charlton
Keyword(s):  
Light Chain ◽  
Sequence Homology ◽  
Myosin Light Chain ◽  
Bioinformatics Analysis ◽  
Arginine Kinase ◽  
Mass Rearing ◽  
Proteomics Data ◽  
Allergenic Proteins ◽  
Food And Feed ◽  
Increasing Demand

Due to the rapid growth of the world’s population and the increasing demand for food, there is an urgent need for alternative, more sustainable sources of protein. Insects have an important role in the diet in some societies and current initiatives are exploring the potential that insects have to offer for the production of food and feed. In this context, the safety implications of both producing and consuming insects are an important aspect to investigate. Here we present a bioinformatics analysis of proteomics data obtained for larvae of four different species of fly to assess the homology of tropomyosin, arginine kinase and myosin light chain with the crustacean orthologous proteins and other known allergenic proteins. The results indicate that the three proteins share homology with known allergens and therefore it is likely that they are also potential allergens. The implications in relation to mass rearing of flies are discussed.

scholarly journals Novel allergen discovery through comprehensive de novo transcriptomic analyses of 5 shrimp species

2020 ◽  
Author(s):  
Shaymaviswanathan Karnaneedi ◽  
Roger Huerlimann ◽  
Elecia B. Johnston ◽  
Roni Nugraha ◽  
Thimo Ruethers ◽  
...  
Keyword(s):  
Calcium Binding ◽  
De Novo ◽  
Arginine Kinase ◽  
Penaeus Monodon ◽  
Clinical Importance ◽  
Major Allergen ◽  
Multiple Sequence ◽  
Alpha Tubulin ◽  
Shrimp Species ◽  
Shellfish Allergy

AbstractShellfish allergy affects up to 2% of the world’s population and persists for life in most patients. The diagnosis of a shellfish allergy, in particular shrimp, is however often challenging due to the similarity of allergenic proteins in other invertebrates. Despite the clinical importance, the complete allergen repertoire of allergy-causing shrimps remains unclear. Here we mine the complete transcriptome of five frequently consumed shrimp species to identify and compare allergens with all known allergen sources. The transcriptomes were assembled de novo from raw RNA-Seq data of the whiteleg shrimp (Litopenaeus vannamei), black tiger shrimp (Penaeus monodon), banana shrimp (Fenneropenaeus merguiensis), king shrimp (Melicertus latisulcatus), and endeavour shrimp (Metapenaeus endeavouri). Trinity was used to assemble the transcriptome, and Transrate and BUSCO applied to verify the assembly. Blast search with the two major allergen databases, WHO/IUIS Allergen Nomenclature and AllergenOnline, successfully identified all seven known crustacean allergens. Salmon was utilised to measure their relative abundance, demonstrating sarcoplasmic calcium-binding protein, arginine kinase and myosin light chain as highly abundant allergens. In addition, the analyses revealed up to 40 unreported allergens in different shrimp species, including heat shock protein (HSP), alpha-tubulin, chymotrypsin, cyclophilin, beta-enolase, aldolase A, and glyceraldehyde-3-phosphate dehydrogenase (G3PD). Multiple sequence alignment, conducted in Jalview 2.1 with Clustal Omega, demonstrated high homology with allergens from other invertebrates including mites and cockroaches. This first transcriptomic analyses of allergens in a major food source provides a valuable genomic resource for investigating shellfish allergens, comparing invertebrate allergens and developing improved diagnostics and novel immunotherapeutics for food allergy.

Allergic Reactions to Food Proteins

2011 ◽  
Vol 81 (23) ◽  
pp. 173-180 ◽  
Author(s):  
Barbara K. Ballmer-Weber
Keyword(s):  
Food Allergies ◽  
Allergic Reactions ◽  
Allergenic Food ◽  
Processed Foods ◽  
Legal Regulations ◽  
Pollen Allergens ◽  
Curative Therapy ◽  
Novel Foods ◽  
Allergenic Proteins ◽  
Adolescents And Adults

Four to eight percent of the population are estimated to be food-allergic. Most food allergies in adolescents and adults are acquired on the basis of cross-reaction to pollen allergens. Theses allergens are ubiquitous in the plant kingdom. Therefore pollen-allergic patients might acquire a multitude of different plant food allergies, and even react to novel foods to which they have never previously been exposed. A curative therapy for food allergy does not yet exist. Food-allergic patients have to rely on strict avoidance diets, The widespread use of industrially processed foods poses a general problem for food-allergic patients. Although the most frequent allergens must be declared openly in the list of ingredients, involuntary contamination with allergy-provoking compounds can occur. The precautionary labelling “may contain” is sometimes applied even if the chance of contamination is very low; on the other hand, foods not declared to contain possible traces of allergenic components may actually contain relevant amounts of allergenic proteins. Switzerland is the only country in Europe with legal regulations on contamination by allergenic food; however, the allowance of 1 g/kg is too high to protect a relevant proportion of food-allergic individuals.

scholarly journals Validation of Real-time PCR Reference Genes of Muscle Metabolism in Harvested Spiny-Cheek Crayfish (Faxonius limosus) Exposed to Seasonal Variation

Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1140
Author(s):  
Natalia Śmietana ◽  
Remigiusz Panicz ◽  
Małgorzata Sobczak ◽  
Piotr Eljasik ◽  
Przemysław Śmietana
Keyword(s):  
Real Time ◽  
Calcium Binding ◽  
Reference Genes ◽  
Target Genes ◽  
Arginine Kinase ◽  
Elongation Factor ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Systematic Analysis ◽  
Key Species

Real-time quantitative reverse transcription PCR (RT-qPCR) is a sensitive and broadly used technique of assessing gene activity. To obtain a reliable result, stably expressed reference genes are essential for normalization of transcripts in various samples. To our knowledge, this is the first systematic analysis of reference genes for normalization of RT-qPCR data in spiny-cheek crayfish (Faxonius limosus). In this study, expression of five candidate reference genes (actb, β-actin; gapdh, glyceraldehyde-3-phosphate dehydrogenase; eif, eukaryotic translation initiation factor 5a; ef-1α, elongation factor-1α; and tub, α-tubulin) in muscle samples from male and female F. limosus in spring and autumn was analyzed. Additionally, the most stable reference genes were used for accurate normalization of five target genes, i.e., tnnc, troponin c; ak, arginine kinase; fr, ferritin; ccbp-23, crustacean calcium-binding protein 23; and actinsk8, skeletal muscle actin 8. Results obtained using the geNorm and NormFinder algorithms showed high consistency, and differences in the activity of the selected actb with eif genes were successfully identified. The spring and autumn activities of the target genes (except ak) in the muscle tissue of males and females differed significantly, showing that both sexes are immensely involved in an array of breeding behaviors in spring, and females intensively recover in the autumn season. Characterization of first reference genes in spiny-cheek crayfish will facilitate more accurate and reliable expression studies in this key species.

scholarly journals Dietary Silicon and Its Impact on Plasma Silicon Levels in the Polish Population

Nutrients ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 980 ◽  
Author(s):  
Anna Prescha ◽  
Katarzyna Zabłocka-Słowińska ◽  
Halina Grajeta
Keyword(s):  
Large Scale ◽  
Graphite Furnace ◽  
Absorption Spectrometry ◽  
Alcoholic Beverages ◽  
Published Data ◽  
Polish Population ◽  
Food Groups ◽  
Dietary Recall ◽  
Median Plasma ◽  
Silicon Level

Silicon in nutritional amounts provides benefits for bone health and cognitive function. The relationship between silicon intake from a common daily diet and silicon blood level has been scarcely elucidated, so far. The aim of this study was to analyze the associations between plasma silicon levels and the total and bioavailable silicon intake—along with the contribution of silicon made by food groups—in a healthy adult Polish population. Si intake was evaluated in 185 healthy adults (94 females and 91 males, aged 20–70) using a 3-day dietary recall and a database on the silicon content in foods, which was based on both previously published data and our own research. Fasting plasma silicon levels were measured in 126 consenting subjects, using graphite furnace atomic absorption spectrometry. The silicon intake in the Polish population differed significantly according to sex, amounting to 24.0 mg/day in women and 27.7 mg/day in men. The median plasma silicon level was 152.3 µg/L having no gender dependency but with a negative correlation with age. Significant correlations were found between plasma silicon level and total and bioavailable silicon intake, as well as water intake in the diet (r = 0.18, p = 0.044; r = 0.23, p = 0.011; r = 0.28, p = 0.002, respectively). Silicon intakes from non-alcoholic beverages, cereal foods, and carotene-rich vegetables were also positively associated with plasma silicon levels. These results may help establish dietary silicon recommendations and formulate practical advice on dietary choices to ensure an appropriate supply of silicon. The outcome of this study, however, needs to be confirmed by large-scale epidemiological investigations.

Phosphorylation of Calmodulin in the First Calcium-Binding Pocket by Myosin Light Chain Kinase

1996 ◽  
Vol 332 (1) ◽  
pp. 101-109 ◽  
Author(s):  
Harold W. Davis ◽  
Dan L. Crimmins ◽  
Richard S. Thoma ◽  
Joe G.N. Garcia
Keyword(s):  
Light Chain ◽  
Calcium Binding ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Binding Pocket

Adjuvant-Driven Redirection of Th2 Immunity Inhibits Allergic Reactions in Murine Models of Food Allergy

2017 ◽  
Vol 139 (2) ◽  
pp. AB175 ◽  
Author(s):  
Jessica J. O'Konek ◽  
Jeffrey J. Landers ◽  
Katarzyna W. Janczak ◽  
Pamela T. Wong ◽  
James R. Baker
Keyword(s):  
Food Allergy ◽  
Allergic Reactions ◽  
Murine Models ◽  
Th2 Immunity
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