scholarly journals Kinetics of in Vitro Response to β-Lactoglobulin in Children Allergic to and Tolerant of Cow’s Milk Protein

2016 ◽  
Vol 26 (4) ◽  
pp. 275-277
Author(s):  
L Chini ◽  
V Pacciani ◽  
S Corrente ◽  
E Monteferrario ◽  
ML Romiti ◽  
...  
Keyword(s):  
Milk Protein ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
In Vitro Response ◽  
Cow’S Milk Protein ◽  
Β Lactoglobulin ◽  
Kinetics Of

In Vitro Cell-Mediated Immunologic Assay for Cow's Milk Allergy

PEDIATRICS ◽  
1980 ◽  
Vol 66 (3) ◽  
pp. 399-402
Author(s):  
Azaria Ashkenazi ◽  
Stanley Levin ◽  
Dalia Idar ◽  
Ayala Or ◽  
Ian Rosenberg ◽  
...  
Keyword(s):  
Normal Control ◽  
Acute Gastroenteritis ◽  
Milk Protein ◽  
Cow’S Milk ◽  
Milk Allergy ◽  
Cow's Milk ◽  
Control Subjects ◽  
Β Lactoglobulin ◽  
In Infants And Children

The production of a lymphokine, the leukocyte-migration-inhibition factor (LIF), by peripheral blood lymphocytes in response to an in vitro challenge with bovine β-lactoglobulin was assayed in infants and children suspected of having allergy to cow's milk protein. of the patients studied, 24 had cow's milk allergy, 24 were normal control subjects, 18 had recovered from milk allergy, 10 were newborns, and 10 were babies suffering from acute gastroenteritis. All patients with milk allergy demonstrated significant LIF production in response to β-lactoglobulin (23.5% ± 6.4%). In the normal control subjects, LIF was 3.1% ± 4.3% (P < .0005). Only two of the 24 control subjects and two of the ten newborns had high-normal values bordering on the positive. None of the ten babies with acute gastroenteritis gave a positive response. Most of the children who had recovered from milk allergy and were ingesting cow's milk had negative assays. This cell-mediated immune assay is shown to be a reliable test for the diagnosis of sensitivity to milk protein in infants and children, and for determining dietary treatment and when this treatment can be safely terminated. In most cases, its use should eliminate the need for the potentially dangerous and ethically questionable provocation test, as well as the need for repeated intestinal biopsies.

scholarly journals Peptide Release after Simulated Infant In Vitro Digestion of Dry Heated Cow’s Milk Protein and Transport of Potentially Immunoreactive Peptides across the Caco-2 Cell Monolayer

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2483 ◽  
Author(s):  
Hannah E. Zenker ◽  
Harry J. Wichers ◽  
Monic M. M. Tomassen ◽  
Sjef Boeren ◽  
Nicolette W. De Jong ◽  
...  
Keyword(s):  
T Cell ◽  
Milk Protein ◽  
Cell Monolayer ◽  
In Vitro Digestion ◽  
Cow’S Milk ◽  
T Cell Epitopes ◽  
Peptide Profile ◽  
Cow's Milk ◽  
Cow’S Milk Protein

Dry heating of cow’s milk protein, as applied in the production of “baked milk”, facilitates the resolution of cow’s milk allergy symptoms upon digestion. The heating and glycation-induced changes of the protein structure can affect both digestibility and immunoreactivity. The immunological consequences may be due to changes in the peptide profile of the digested dry heated milk protein. Therefore, cow’s milk protein powder was heated at low temperature (60 °C) and high temperature (130 °C) and applied to simulated infant in vitro digestion. Digestion-derived peptides after 10 min and 60 min in the intestinal phase were measured using LC-MS/MS. Moreover, digests after 10 min intestinal digestion were applied to a Caco-2 cell monolayer. T-cell epitopes were analysed using prediction software, while specific immunoglobin E (sIgE) binding epitopes were identified based on the existing literature. The largest number of sIgE binding epitopes was found in unheated samples, while T-cell epitopes were equally represented in all samples. Transport of glycated peptide indicated a preference for glucosyl lysine and lactosyl-lysine-modified peptides, while transport of peptides containing epitope structures was limited. This showed that the release of immunoreactive peptides can be affected by the applied heating conditions; however, availability of peptides containing epitopes might be limited.

scholarly journals In vitro diagnosis of cow's milk protein sensitive enteropathy by organ culture method.

Gut ◽  
1981 ◽  
Vol 22 (3) ◽  
pp. 199-202 ◽  
Author(s):  
N Iyngkaran ◽  
M Yadav ◽  
S Balabaskaran ◽  
E Sumithran
Keyword(s):  
Organ Culture ◽  
Milk Protein ◽  
Culture Method ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Cow’S Milk Protein ◽  
In Vitro Diagnosis

scholarly journals 52 In Vitro Evidence of Cow's Milk Protein Sensitisation in Neonatal Necrotising Enterocolitis

2004 ◽  
Vol 56 (3) ◽  
pp. 473-473
Author(s):  
S L Chuang ◽  
P Hayes ◽  
E Ogundipe ◽  
N Modi ◽  
M Haddad ◽  
...  
Keyword(s):  
Milk Protein ◽  
Necrotising Enterocolitis ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Neonatal Necrotising Enterocolitis ◽  
Cow’S Milk Protein

Cow’s milk protein β-lactoglobulin confers resilience against allergy by targeting complexed iron into immune cells

2021 ◽  
Vol 147 (1) ◽  
pp. 321-334.e4 ◽  
Author(s):  
Franziska Roth-Walter ◽  
Sheriene Moussa Afify ◽  
Luis F. Pacios ◽  
Bart R. Blokhuis ◽  
Frank Redegeld ◽  
...  
Keyword(s):  
Immune Cells ◽  
Milk Protein ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Cow’S Milk Protein ◽  
Β Lactoglobulin
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