scholarly journals MeloVir : Bepaling van het virulentiespectrum van Meloidogyne chitwoodi populaties t.b.v resistentie onderzoek

2021 ◽  
Author(s):  
M.G. Teklu ◽  
◽  
T.H. Been ◽  
C.H. Schomaker ◽  
L.P.G. Molendijk ◽  
...  
Keyword(s):  
Meloidogyne Chitwoodi

Population fluctuations of root-knot nematodes Meloidogyne chitwoodi and M. hapla under field conditions

2021 ◽  
Author(s):  
Emre Evlice ◽  
Halil Toktay ◽  
Gökhan Yatkın ◽  
Fatma Dolunay Erdoğuş ◽  
Mustafa İmren
Keyword(s):  
Field Conditions ◽  
Population Fluctuations ◽  
Meloidogyne Chitwoodi ◽  
Root Knot Nematodes

scholarly journals Independent Resistant Reactions Expressed in Root and Tuber of Potato Breeding Lines with Introgressed Resistance to Meloidogyne chitwoodi

2009 ◽  
Vol 99 (9) ◽  
pp. 1085-1089 ◽  
Author(s):  
C. R. Brown ◽  
H. Mojtahedi ◽  
L.-H. Zhang ◽  
E. Riga
Keyword(s):  
Genetic Factors ◽  
Dominant Gene ◽  
Breeding Line ◽  
Single Dominant Gene ◽  
Solanum Bulbocastanum ◽  
Chromosome 11 ◽  
Meloidogyne Chitwoodi ◽  
Tuber Resistance ◽  
Advanced Backcross ◽  
Resistance Breaking

Resistance to Meloidogyne chitwoodi was introgressed from Solanum bulbocastanum into the cultivated gene pool of potato. A single dominant gene is responsible for resistance to race 1 reproduction on the root system. An additional form of resistance was discovered in certain advanced backcross clones. A BC5 clone, PA99N82-4, resisted invasion of tubers by available nematode juveniles whether supplied by weeds or challenged by several root resistance-breaking pathotypes. This tuber resistance is inherited as a single dominant gene and is linked to RMc1(blb). Because this gene has been mapped to chromosome 11, tuber resistance genetic factors are inferred to be on the same chromosome in coupling phase. Among 153 progeny derived from crosses with PA99N82-4, 42 recombinants, comprising both resistant root/susceptible tuber and susceptible root/resistant tubers, were found while other progeny were doubly resistant (like PA99N82-4) or doubly susceptible. Therefore, the existence of two linked genetic factors controlling independently expressed traits is confirmed. The combination of the two phenotypes is likely to be a sufficient level of resistance to avoid tuber damage from circumstances that provide exogenous juveniles proximal to the tubers in the soil. These factors are weed hosts of M. chitwoodi host races and pathotypes of M. chitwoodi that overcome RMc1(blb). Under field conditions, where a resistance-breaking pathotype of M. chitwoodi was present, tuber-resistant PA99N82-4 breeding line produced tubers which were commercially acceptable and not culled. A related breeding line, root resistant but tuber susceptible, and Russet Burbank were severely tuber damaged and commercially unacceptable.

The MeloTuber Test: a real-time TaqMan® PCR-based assay to detect the root-knot nematodes Meloidogyne chitwoodi and M. fallax directly in potato tubers

EPPO Bulletin ◽  
2014 ◽  
Vol 44 (2) ◽  
pp. 166-175 ◽  
Author(s):  
E. G. de Haan ◽  
C. C. E. M. Dekker ◽  
W. I. L. Tameling ◽  
L. J. M. F. den Nijs ◽  
G. W. van den Bovenkamp ◽  
...  
Keyword(s):  
Real Time ◽  
Potato Tubers ◽  
Meloidogyne Chitwoodi ◽  
Root Knot Nematodes

scholarly journals Interspecific Hybridization of Meiotic Parthenogenetic Meloidogyne chitwoodi and M. fallax

1997 ◽  
Vol 87 (10) ◽  
pp. 1061-1066 ◽  
Author(s):  
J. G. van der Beek ◽  
G. Karssen
Keyword(s):  
Malate Dehydrogenase ◽  
Interspecific Hybridization ◽  
Genetic Material ◽  
Conclusive Evidence ◽  
Species Differentiation ◽  
Mating Experiment ◽  
Meloidogyne Chitwoodi ◽  
Isozyme Electrophoresis ◽  
Parental Type ◽  
Clear Differentiation

Hybridization between two meiotic parthenogenetic species of root-knot nematodes, Meloidogyne chitwoodi and M. fallax, was investigated in two different crossing experiments on tomato plants grown in sand. The first experiment was a controlled cross between the two species. The second experiment was a bulk mating in a 1:1 mixture of two isolates. The haploid chromosome number of the parental isolates was n = 18. Successful interspecific hybridization was obtained, and the resulting hybrids produced egg masses. In eggs, cell division was observed, but most of them were without clear differentiation and consequently were sterile. Hatched F2 juveniles were small in number, not viable, and showed morphological distortions. In the progeny of the isolate mixture of the bulk mating experiment, parental-type females of the two isolates were present in equal numbers, and 10% of all females were nonviable hybrids. Similar ratios of parental-type and hybrid females were detected in roots of test plants grown in soil from a field sample that contained a mixture of M. chitwoodi and M. fallax populations. In the controlled cross experiment, isozyme electrophoresis of malate dehydrogenase was applied to distinguish the two species and their hybrids. In the bulk mating experiment, malate dehydrogenase, esterase, and glucose 6-phosphate dehydrogenase were used as markers, two by two simultaneously on the same individual females, providing conclusive evidence for the occurrence of hybrids. This is the first report on interspecific hybridization in Meloidogyne. The possible role of interspecific hybridization in species differentiation and interspecific exchange of genetic material within Meloidogyne is discussed.

scholarly journals Durable resistance against Meloidogyne chitwoodi and M. fallax

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 711-713
Author(s):  
F.C. Zoon ◽  
W. Golinowski ◽  
R. Janssen ◽  
D. Mugniéry ◽  
M.S. Phillips ◽  
...  
Keyword(s):  
Production Systems ◽  
Durable Resistance ◽  
Resistance Management ◽  
Resistance Mechanisms ◽  
Genetic Maps ◽  
Meloidogyne Chitwoodi ◽  
First Results ◽  
Stability Of Resistance ◽  
Nematode Virulence ◽  
The Stability

The EU-funded Project QLRT-1999-1462 DREAM (Durable Resistance Against Meloidogyne) aims to contribute to sustainable production systems by developing a strategy for durable resistance management for two polyphagous quarantine root-knot nematodes Meloidogyne chitwoodi and M. fallax. The objective will be achieved by integrating expertise in breeding, nematology, botany and molecular biology. The project combines three areas of research: 1. Identification and incorporation of resistance in important arable crops: potato, pepper, ryegrass and fodder radish, 2. Study of variation in nematode virulence and of durability of the resistance, and 3. Optimising of production systems by rotation schemes. The main results expected are: resistant germplasm, characterised pathogen collections, breeding methods, knowledge of the stability of resistance, molecular markers linked to resistance and (a)virulence, resistance mechanisms and genes, genetic maps, and improved rotation schemes. The strategy and some first results will be discussed.

Estimation of partial resistance in potato genotypes against Meloidogyne chitwoodi

Nematology ◽  
2011 ◽  
Vol 13 (4) ◽  
pp. 477-489 ◽  
Author(s):  
Thomas Been ◽  
Corrie Schomaker ◽  
Patrick Norshie
Keyword(s):  
Partial Resistance ◽  
Damage Threshold ◽  
Internal Quality ◽  
Yield Reduction ◽  
Initial Population ◽  
Multiplication Rate ◽  
Population Densities ◽  
Meloidogyne Chitwoodi ◽  
Industrial Processing ◽  
Final Population

AbstractThree new potato genotypes, designated AR 04-4107, AR 04-4096 and AR 04-4098, with resistance towards Meloidogyne chitwoodi, and the susceptible cv. Désirée were grown at a range of population densities of M. chitwoodi in a climate-controlled glasshouse in order to establish the presence and degree of partial resistance. Tuber parts of about 12 g were planted at densities (Pi) of 0, 0.5, 1, 2, 4, 8, 16, 32, 64, 128 and 256 second-stage juveniles (J2) (g dry soil)−1. The plants were allowed to grow for a period of 105 days. Tomato cv. Moneymaker was included and inoculated at Pi = 2 J2 (g soil)−1 to verify the quality of the inoculum by measuring the multiplication rate. Plant height was measured weekly over 11 weeks. At harvest, fresh shoot, root and tuber weights, and number of tubers were measured to express yield. Final population densities (Pf) were calculated as the total number of nematodes found in soil and roots. Tubers were scored for visible symptoms and a root-knot index was calculated. The relation between pre-plant population densities (Pi) and nematode densities at harvest (Pf) was fitted using R. The multiplication rate a of M. chitwoodi on AR 04-4107, AR 04-4096, AR 04-4098 and cv. Désirée was 0.55, 0.27, 0.91 and 32, respectively. Partial resistance rsa of AR 04-4107, AR 04-4096 and AR 04-4098 was 1.7%, 0.8% and 2.8%, respectively. Partial resistance expressed as rsM was 0.2%, 0.2% and 0.1%, respectively. It can be concluded that AR 04-4107, AR 04-4096 and AR 04-4098 are strongly partially resistant to M. chitwoodi. Also, the population dynamics curves run almost parallel between both the tested genotypes and the reference cultivar, indicating that a simple and cheap partial resistance test is feasible. When tuber yields were fitted to the Seinhorst model for yield reduction, cv. Désirée showed a minimum yield (m) of 0.86, while all three resistant genotypes suffered no yields losses at all (m = 1), which indicates that the observed resistance was associated with tolerance. As a result of the remarkably high partial resistance, quality damage was low compared with cv. Désirée. The root-knot index, which takes into account internal quality damage of the potato tuber, was below 10 for all genotypes with partial resistance, the lower damage threshold used for industrial processing of consumption potatoes. Visible symptoms on the tuber skin were absent up to densities of 32 J2 (g soil)−1 for genotypes AR 04-4098 and AR 04-4096 and 2 J2 (g soil)−1 for AR 04-4107, and significantly reduced at higher densities when compared with the susceptible cv. Désirée. However, when tuber peels were investigated, egg masses were detected in tubers at almost all initial population densities.

Milkweed seedmeal: a control for Meloidogyne chitwoodi on potatoes

1999 ◽  
Vol 9 (2) ◽  
pp. 145-150 ◽  
Author(s):  
R.E Harry-O'kuru ◽  
H Mojtahedi ◽  
S.F Vaughn ◽  
P.F Dowd ◽  
G.S Santo ◽  
...  
Keyword(s):  
Meloidogyne Chitwoodi

scholarly journals Correction to: Transcriptome profiling of resistance response to Meloidogyne chitwoodi introgressed from wild species Solanum bulbocastanum into cultivated potato

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Sapinder Bali ◽  
Kelly Vining ◽  
Cynthia Gleason ◽  
Hassan Majtahedi ◽  
Charles R. Brown ◽  
...  
Keyword(s):  
Wild Species ◽  
Transcriptome Profiling ◽  
Solanum Bulbocastanum ◽  
Resistance Response ◽  
Meloidogyne Chitwoodi ◽  
Cultivated Potato

Following the publication of this article [1], the authors noted an error in Figure 11.

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