scholarly journals Toxicity of 3 water samples tested with the Algae growth inhibition test using Raphidocelis subcapitata : Test report

2019 ◽  
Author(s):  
M.C. Keur ◽  
◽  
N.H.B.M. Kaag
Keyword(s):  
Growth Inhibition ◽  
Water Samples ◽  
Inhibition Test ◽  
Test Report ◽  
Growth Inhibition Test ◽  
Raphidocelis Subcapitata ◽  
Algae Growth ◽  
Algae Growth Inhibition Test

scholarly journals Comparative genome analysis of test algal strain NIVA-CHL1 (Raphidocelis subcapitata) maintained in microalgal culture collections worldwide

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0241889
Author(s):  
Takahiro Yamagishi ◽  
Haruyo Yamaguchi ◽  
Shigekatsu Suzuki ◽  
Mayumi Yoshikawa ◽  
Ian Jameson ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Protein Function ◽  
Algal Growth ◽  
Inhibition Test ◽  
Nucleotide Polymorphisms ◽  
Loss Of Function ◽  
Culture Collections ◽  
Growth Inhibition Test ◽  
Raphidocelis Subcapitata ◽  
Microalgal Culture

Raphidocelis subcapitata is one of the most frequently used species for algal growth inhibition tests. Accordingly, many microalgal culture collections worldwide maintain R. subcapitata for distribution to users. All R. subcapitata strains maintained in these collections are derived from the same cultured strain, NIVA-CHL1. However, considering that 61 years have passed since this strain was isolated, we suspected that NIVA-CHL1 in culture collections might have acquired various mutations. In this study, we compared the genome sequences among NIVA-CHL1 from 8 microalgal culture collections and one laboratory in Japan to evaluate the presence of mutations. We found single-nucleotide polymorphisms or indels at 19,576 to 28,212 sites per strain in comparison with the genome sequence of R. subcapitata NIES-35, maintained at the National Institute for Environmental Studies, Tsukuba, Japan. These mutations were detected not only in non-coding but also in coding regions; some of the latter mutations may affect protein function. In growth inhibition test with 3,5-dichlorophenol, EC50 values varied 2.6-fold among the 9 strains. In the ATCC 22662–2 and CCAP 278/4 strains, we also detected a mutation in the gene encoding small-conductance mechanosensitive ion channel, which may lead to protein truncation and loss of function. Growth inhibition test with sodium chloride suggested that osmotic regulation has changed in ATCC 22662–2 and CCAP 278/4 in comparison with NIES-35.

Dependence of welding fume particle toxicity on electrode type and current intensity assessed by microalgae growth inhibition test

2019 ◽  
Vol 179 ◽  
pp. 108818 ◽  
Author(s):  
Konstantin Kirichenko ◽  
Alexander Zakharenko ◽  
Konstantin Pikula ◽  
Vladimir Chaika ◽  
Zhanna Markina ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Inhibition Test ◽  
Current Intensity ◽  
Welding Fume ◽  
Growth Inhibition Test ◽  
Microalgae Growth
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