Development and Application of Whole Cell and Intact Outer-Membranes for Double Electron-Electron Resonance on Membrane Proteins

2016 ◽  
Author(s):  
Arthur Sikora
Keyword(s):  
Membrane Proteins ◽  
Whole Cell ◽  
Electron Resonance ◽  
Outer Membranes ◽  
Double Electron ◽  
Double Electron Electron Resonance

Artefact suppression in 5-pulse double electron electron resonance for distance distribution measurements

2017 ◽  
Vol 19 (24) ◽  
pp. 15766-15779 ◽  
Author(s):  
Frauke D. Breitgoff ◽  
Janne Soetbeer ◽  
Andrin Doll ◽  
Gunnar Jeschke ◽  
Yevhen O. Polyhach
Keyword(s):  
Membrane Proteins ◽  
Data Processing ◽  
Spin Relaxation ◽  
Electron Spin ◽  
Distance Distribution ◽  
Spectral Purity ◽  
Electron Resonance ◽  
Electron Spin Relaxation ◽  
Double Electron ◽  
Double Electron Electron Resonance

Long dipolar evolution data for membrane proteins with exponential electron spin relaxation can be achieved with spectral purity sufficient for robust data processing.

Direct Observation of Chain Lengths and Conformations in Oligofluorene Distributions from Controlled Polymerization by Double Electron-Electron Resonance

2019 ◽  
Author(s):  
Dennis Bücker ◽  
Annika Sickinger ◽  
Julian D. Ruiz Perez ◽  
Manuel Oestringer ◽  
Stefan Mecking ◽  
...  
Keyword(s):  
Chain Length ◽  
Length Distribution ◽  
Catalyst System ◽  
Chain Conformation ◽  
Controlled Polymerization ◽  
Chain Length Distribution ◽  
Electron Resonance ◽  
Double Electron ◽  
The Individual ◽  
Double Electron Electron Resonance

Synthetic polymers are mixtures of different length chains, and their chain length and chain conformation is often experimentally characterized by ensemble averages. We demonstrate that Double-Electron-Electron-Resonance (DEER) spectroscopy can reveal the chain length distribution, and chain conformation and flexibility of the individual n-mers in oligo-(9,9-dioctylfluorene) from controlled Suzuki-Miyaura Coupling Polymerization (cSMCP). The required spin-labeled chain ends were introduced efficiently via a TEMPO-substituted initiator and chain terminating agent, respectively, with an in situ catalyst system. Individual precise chain length oligomers as reference materials were obtained by a stepwise approach. Chain length distribution, chain conformation and flexibility can also be accessed within poly(fluorene) nanoparticles.

In-Cell Double Electron–Electron Resonance at Nanomolar Protein Concentrations

2021 ◽  
pp. 3679-3684
Author(s):  
Svetlana Kucher ◽  
Christina Elsner ◽  
Mariya Safonova ◽  
Stefano Maffini ◽  
Enrica Bordignon
Keyword(s):  
Electron Resonance ◽  
Double Electron ◽  
Double Electron Electron Resonance

Distance Determination in Proteins insideXenopus laevisOocytes by Double Electron−Electron Resonance Experiments

2010 ◽  
Vol 132 (24) ◽  
pp. 8228-8229 ◽  
Author(s):  
Ryuji Igarashi ◽  
Tomomi Sakai ◽  
Hideyuki Hara ◽  
Takeshi Tenno ◽  
Toshiaki Tanaka ◽  
...  
Keyword(s):  
Distance Determination ◽  
Electron Resonance ◽  
Double Electron ◽  
Double Electron Electron Resonance

scholarly journals Shigella flexneri Spa15 Crystal Structure Verified in Solution by Double Electron Electron Resonance

2011 ◽  
Vol 405 (2) ◽  
pp. 427-435 ◽  
Author(s):  
James E.D. Lillington ◽  
Janet E. Lovett ◽  
Steven Johnson ◽  
Pietro Roversi ◽  
Christiane R. Timmel ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Shigella Flexneri ◽  
Electron Resonance ◽  
Double Electron ◽  
Double Electron Electron Resonance
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