scholarly journals In Vitro and In Vivo Characterization of Novel Small-Molecule Alzheimer's Disease Amyloid-Beta Therapies

2014 ◽  
Author(s):  
Jacob Irwin
Keyword(s):  
Small Molecule ◽  
Amyloid Beta ◽  
In Vivo Characterization

Abstract 326: Discovery and in vitro and in vivo characterization of a novel, small-molecule Ras inhibitor class

2016 ◽  
Author(s):  
Adam B. Keeton ◽  
Bing Zhu ◽  
Kevin J. Lee ◽  
Joshua C. Canzoneri ◽  
Sara C. Sigler ◽  
...  
Keyword(s):  
Small Molecule ◽  
In Vivo Characterization

In vitro and in vivo characterization of SGI-1252, a small molecule inhibitor of JAK2

2011 ◽  
Vol 39 (1) ◽  
pp. 14-25 ◽  
Author(s):  
Kausar Begam Riaz Ahmed ◽  
Steven L. Warner ◽  
Andrew Chen ◽  
Eric S. Gourley ◽  
Xiaohui Liu ◽  
...  
Keyword(s):  
Small Molecule ◽  
Small Molecule Inhibitor ◽  
Molecule Inhibitor ◽  
In Vivo Characterization

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

In vitro and in vivo characterization of graphene oxide coated porcine bone granules

Carbon ◽  
2016 ◽  
Vol 103 ◽  
pp. 291-298 ◽  
Author(s):  
Valeria Ettorre ◽  
Patrizia De Marco ◽  
Susi Zara ◽  
Vittoria Perrotti ◽  
Antonio Scarano ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
In Vivo Characterization

P.3.d.011 In vitro and in vivo characterization of glycine transporter type-1 by two inhibitors, Org-24461 and NFPS

2006 ◽  
Vol 16 ◽  
pp. S435 ◽  
Author(s):  
B. Marko ◽  
G. Szabo ◽  
S. Udvari ◽  
M. Agoston ◽  
E. Szabo ◽  
...  
Keyword(s):  
Glycine Transporter ◽  
In Vivo Characterization

scholarly journals Correction to “Improving Paclitaxel Delivery: In Vitro and In Vivo Characterization of PEGylated Polyphosphoester-Based Nanocarriers”

2015 ◽  
Vol 137 (21) ◽  
pp. 6972-6972 ◽  
Author(s):  
Fuwu Zhang ◽  
Shiyi Zhang ◽  
Stephanie F. Pollack ◽  
Richen Li ◽  
Amelia M. Gonzalez ◽  
...  
Keyword(s):  
In Vivo Characterization

Synthesis, in vitro and in vivo characterization of novel 99mTc-‘4+1’-labeled 5-nitroimidazole derivatives as potential agents for imaging hypoxia

2012 ◽  
Vol 39 (5) ◽  
pp. 679-686 ◽  
Author(s):  
Javier Giglio ◽  
Soledad Fernández ◽  
Hans-Jürgen Pietzsch ◽  
Sylvia Dematteis ◽  
María Moreno ◽  
...  
Keyword(s):  
In Vivo Characterization
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