scholarly journals HIV, ADAR Editing, The Rev-RRE Pathway and Antisense RNA-mediated Inhibition

2017 ◽  
Author(s):  
Siripong Tongjai
Keyword(s):  
Antisense Rna ◽  
Adar Editing

Antisense RNA-mediated GmFAD2-1B Gene Silencing Enhances Accumulation of Oleic Acid in Transgenic Soybean Seeds

2017 ◽  
Vol 43 (11) ◽  
pp. 1588 ◽  
Author(s):  
Jing YANG ◽  
Guo-Jie XING ◽  
Lu NIU ◽  
Hong-Li HE ◽  
Qian DU ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Gene Silencing ◽  
Antisense Rna ◽  
Soybean Seeds ◽  
Transgenic Soybean

Permanent Inhibition of Angiotensinogen Synthesis by Antisense RNA Expression

Hypertension ◽  
1996 ◽  
Vol 27 (3) ◽  
pp. 508-513 ◽  
Author(s):  
Martina Schinke ◽  
Manfred Böhm ◽  
Giampiero Bricca ◽  
Detlev Ganten ◽  
Michael Bader
Keyword(s):  
Antisense Rna ◽  
Rna Expression

scholarly journals Natural Antisense Transcript PEBP1P3 Regulates the RNA Expression, DNA Methylation and Histone Modification of CD45 Gene

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 759
Author(s):  
Zhongjing Su ◽  
Guangyu Liu ◽  
Bin Zhang ◽  
Ze Lin ◽  
Dongyang Huang
Keyword(s):  
Dna Methylation ◽  
Alternative Splicing ◽  
Histone Modification ◽  
Antisense Rna ◽  
Noncoding Rna ◽  
Antisense Transcript ◽  
Natural Antisense Transcript ◽  
Regulation Of Expression ◽  
Splicing Isoforms ◽  
Intron 2

The leukocyte common antigen CD45 is a transmembrane phosphatase expressed on all nucleated hemopoietic cells, and the expression levels of its splicing isoforms are closely related to the development and function of lymphocytes. PEBP1P3 is a natural antisense transcript from the opposite strand of CD45 intron 2 and is predicted to be a noncoding RNA. The genotype-tissue expression and quantitative PCR data suggested that PEBP1P3 might be involved in the regulation of expression of CD45 splicing isoforms. To explore the regulatory mechanism of PEBP1P3 in CD45 expression, DNA methylation and histone modification were detected by bisulfate sequencing PCR and chromatin immunoprecipitation assays, respectively. The results showed that after the antisense RNA PEBP1P3 was knocked down by RNA interference, the DNA methylation of CD45 intron 2 was decreased and histone H3K9 and H3K36 trimethylation at the alternative splicing exons of CD45 DNA was increased. Knockdown of PEBP1P3 also increased the binding levels of chromatin conformation organizer CTCF at intron 2 and the alternative splicing exons of CD45. The present results indicate that the natural antisense RNA PEBP1P3 regulated the alternative splicing of CD45 RNA, and that might be correlated with the regulation of histone modification and DNA methylation.

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