scholarly journals The antioxidant status at patients with a thyreotoxicosis syndrome at the combined therapy with epifamin

2010 ◽  
Vol 56 (3) ◽  
pp. 397-403 ◽  
Author(s):  
S.S. Popov ◽  
A.N. Pashkov ◽  
V.I. Zoloedov ◽  
T.N. Popova ◽  
T.I. Rakhmanova
Keyword(s):  
Free Radical ◽  
Blood Serum ◽  
Catalase Activity ◽  
Combined Therapy ◽  
Free Radical Oxidation ◽  
Traditional Treatment ◽  
Group 13 ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Radical Processes

The work purpose was research of degree of free-radical processes intensity and catalase activity in blood serum of patients with a thyreotoxicosis syndrome at traditional treatment and the combined therapy with epifamin. Patients (n=25) have been divided into 2 groups: the first group of patients - 12 persons who are on traditional treatment (antithyreoid drugs, β-adrenoblockers); the second group - 13 persons, are padding to basic therapy received epifamin on 1 tablet (10 mg) 3 times a day 10-15 minutes prior to meal within 7 days. Patients with thyreotoxicosis syndrome had an intensification of free-radical oxidation that was traced at measurement of biochemiluminescence parameters in blood serum. At the combined therapy with epifamin less expressed intensity of free-radical processes, and also increasing of the general antioxidant activity of an organism that did not descend at traditional treatment was observed. At carrying out of standard therapy of thyreotoxicosis syndrome, including application of antithyreoid drugs and β-adrenoblockers, catalase activity decreased, that unfavorable affected antioxidant potential of an organism. At the combined therapy with epifamin level of catalase activity, on the contrary, increased, that could influence positively on antioxidant protection of an organism.

Adaptogenic correction of free radical brain damage in subchronic exposure to sodium fl uoride

2020 ◽  
pp. 381-386
Author(s):  
A. G. Zhukova ◽  
L. G. Gorokhova ◽  
A. S. Kazitskaya ◽  
T. K. Yadykina ◽  
N. N. Mikhailova ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Free Radical ◽  
Brain Tissue ◽  
Free Radical Oxidation ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Subchronic Exposure ◽  
Antioxidant Defense Enzymes ◽  
Radical Processes ◽  
Complex Drug

Introduction. Fluorine compounds in small doses, but with prolonged exposure, cause various disorders in organs at the cellular and molecular levels. Activation of free-radical processes plays an important role in the damaging eff ect of fl uorides. Th erefore, one of the most eff ective ways to limit fl uorine-induced damage is to directly aff ect free-radical processes using herbal preparations with antioxidant properties.The aim of the study is to study the eff ect of a dihydroquercetin-based drug on the activity of free radical processes in brain tissue under subchronic exposure to sodium fl uoride (NaF).Materials and methods. Th e work was performed on white male laboratory rats weighing 200-250 g. Th e rats were divided into 3 groups: 1 — control; 2 — rats with chronic exposure to sodium fl uoride (NaF) for 9 weeks; 3 — rats receiving a NAF solution with simultaneous administration of a complex drug based on dihydroquercetin at a dose of 3 mg/kg in 1% starch gel for 3, 6 and 9 weeks. The activity of free radical oxidation and antioxidant defense enzymes — superoxide dismutase (SOD) and catalase-was determined in the cerebral cortex. Th e level of expression of hypoxia-induced transcription factor HIF — 1A and inducible forms of proteins HSP72 and HSP32 were determined in the cytosolic fraction of brain tissue.Results. In the early stages of subchronic fl uoride exposure (1-3 weeks), the expression of protective proteins HIF-1α, HSP72, HSP32 and catalase was shown in the rat cortex, as a result of which the activity of free-radical processes was maintained at the control level. An increase in the timing of fl uoride intake to 9 weeks led to a decrease in antioxidant protection and signifi cant activation of free radical oxidation in brain tissue. Daily administration of a complex drug with dihydroquercetin for 3, 6 and 9 weeks to rats with subchronic fl uoride exposure led to a decrease in the severity of pro- and antioxidant balance disorders in the cerebral cortex. At the same time, the greatest protective eff ect of dihydroquercetin with fl uoride exposure was manifested by the 9th week of its administration.Conclusions. When subchronic intake of fl uorides in the body, the drug based on dihydroquercetin has a neuroprotective eff ect, which is manifested by an increase in the activity of antioxidant enzymes of fr ee radical oxidation and catalase and the resistance of the cortex to induced fr ee radical oxidation.

scholarly journals Expression of Glutathione Peroxidase and Glutathione Reductase and Level of Free Radical Processes under Toxic Hepatitis in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Igor Y. Iskusnykh ◽  
Tatyana N. Popova ◽  
Aleksander A. Agarkov ◽  
Miguel Â. A. Pinheiro de Carvalho ◽  
Stanislav G. Rjevskiy
Keyword(s):  
Free Radical ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Toxic Hepatitis ◽  
Free Radical Oxidation ◽  
Inflammatory Cells ◽  
Maximum Increase ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Radical Processes

Correlation between intensity of free radical processes estimated by biochemiluminesce parameters, content of lipoperoxidation products, and changes of glutathione peroxidase (GP, EC 1.11.1.9) and glutathione reductase (GR, EC 1.6.4.2) activities at rats liver injury, after 12, 36, 70, 96, 110, and 125 hours & tetrachloromethane administration have been investigated. The histological examination of the liver sections of rats showed that prominent hepatocytes with marked vacuolisation and inflammatory cells which were arranged around the necrotic tissue are more at 96 h after exposure to CCl4. Moreover maximum increase in GR and GP activities, 2.1 and 2.5 times, respectively, was observed at 96 h after exposure to CCl4, what coincided with the maximum of free radical oxidation processes. Using a combination of reverse transcription and real-time polymerase chain reaction, expression of the glutathione peroxidase and glutathione reductase genes (Gpx1 and Gsr) was analyzed by the determination of their respective mRNAs in the rat liver tissue under toxic hepatitis conditions. The analyses of Gpx1 and Gsr expression revealed that the transcript levels increased in 2.5- and 3.0-folds, respectively. Western blot analysis revealed that the amounts of hepatic Gpx1 and Gsr proteins increased considerably after CCl4administration. It can be proposed that the overexpression of these enzymes could be a mechanism of enhancement of hepatocytes tolerance to oxidative stress.

scholarly journals Вплив чотирихлористого вуглецю на стан вільнорадикальних процесів організму щурів та морфологічні зміни у товстій кишці

2017 ◽  
Author(s):  
A. M. Pryshlyak ◽  
S. I. Yavorska ◽  
Т. К. Holovatа ◽  
B. Y. Reminetskyy
Keyword(s):  
Free Radical ◽  
Visual Analysis ◽  
White Male ◽  
Free Radical Oxidation ◽  
Male Rats ◽  
Pure Substance ◽  
Diene Conjugates ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Radical Processes

Introduction. Increased admission of herbal compounds in organism of humans and animals can trigger the development of functional disorders and pathological changes of the digestive system and in particular the colon.The aim of the study – tо evaluate the state of free radical processes and morphological changes in the colon with toxic experimental animals exposed by carbon tetrachloride.Research Methods. The study was performed on 44 white male rats weighing 180–200 g which were divided into 4 groups: group 1 – control (almost healthy animals); group 2–4 – rats with simulated toxic lesion by CCl4. Intragastric CCl4 was administered in 50.0 % oil solution at a rate of 0.2 ml of pure substance per100 g of body weight. The intensity of free radical processes evaluated with the level of malondialdehyde and diene conjugates, determined using spectrophotometric techniques; antioxidant system – with the activity of catalase in blood. According to the conventional method histological preparations were made. For qualitative and morphometric analysis system of a visual analysis of images using camera Vision Color CCD program and Inter Video Win DVR UTHSCSA Image Tool were used.Results and Discussion. The results shows that animals carbon tetrachloride intoxication is accompanied by activation of free radical oxidation of lipids, as evidenced by the increase in blood serum of animal content as malondialdehyde (MDA) and diene conjugates (DC) throughout the experiment, with the maximum concentration observed in malondialdehyde 7 days after poisoning. Under the influence of CC14 catalase activity increases in serum at the second, seventh and fourteenth days of the experiment in 1.3, 1.2 and 2.4 times respectively. Morphometric techniques revealed a significant restructuring of the mucosa, submucosa, muscular layer and serous membrane of the colon, accompanied by severe vascular disorders of alterative, infiltrative processes and changes of morphometric parameters of the colon’s wall structures at tissue and organ levels.Conclusions. In the case of CCl4 damage, the processes of free radical oxidation take place, the accumulation of toxic products of lipid peroxidation in the blood and the weakening of the enzyme system of antioxidant protection occur. A significant structural and morphometric reconstruction of the mucosa, submucosa, muscular and serous membranes of the colon has been established.

scholarly journals Intensity of cardiac free-radicals processes and expression of glutathione peroxidase and glutathione reductase genes in rats with adrenaline

2012 ◽  
Vol 58 (5) ◽  
pp. 530-538 ◽  
Author(s):  
I.Y. Iskusnykh ◽  
T.N. Popova ◽  
O.S. Musharova
Keyword(s):  
Free Radical ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Free Radical Oxidation ◽  
Pathological Process ◽  
Mrna Levels ◽  
Maximal Increase ◽  
Radical Oxidation ◽  
Polymerase Chain ◽  
Radical Processes

The correlation between changes in activities of glutathione peroxidase and glutathione reductase in heart of rats during development of adrenaline myocarditis and intensity of free radical processes estimated by biochemiluminesce parameters and the content of lipoperoxidation products was demonstrated. The maximal increase of glutathione peroxidase and glutathione reductase activities (in 1.8 and 1.4 times accordingly) was observed t 24 h after the development of the pathological process; this coincided with the maximum intensity of prosesses of free radical oxidation. Using combination of reverse transcriptions with real-time polymerase chain reaction the cardiac mRNA levels of glutathione peroxidase and glutathione reductase genes were determined during the development of adrenaline myocarditis in rats. Analysis of expression of glutathione peroxidase and glutathione reductase genes showed, that the level of this transcripts demonstrated 2,8- and 7,3- increase in rats with adrenaline myocarditis, respectively. Obviously, overexpression of these enzymes can increase the resistance of cardiomyocites to oxidative stress.

scholarly journals NITRIC OXIDE AND ALLYL ALCOHOL INDUCED HEPATOTOXICITY

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
M. M. Korda
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Blood Serum ◽  
Allyl Alcohol ◽  
Calcium Ionophore ◽  
Free Radical Oxidation ◽  
No Synthase ◽  
Nitrate Content ◽  
Radical Oxidation ◽  
Nos Inhibitors

<p><strong>Background.</strong> Nitric oxide (NO) is an important mediator of hepatotoxicity. NO in liver can be derived from two sources: (1) constitutive NO synthase (eNOS) in endothelial cells, and (2) inducible NO synthase (iNOS) in hepatocytes and Kupffer cells.<br /><strong>Objectives.</strong> The present study was aimed to examine the effect of nonselective NOS inhibitor (L-NAME) and selective iNOS inhibitor (1400W) on the development of allyl alcohol (AA) induced hepatitis in rats.<br /><strong>Methods.</strong> Male Wistar rats were treated with intraperitoneal injection of saline or AA and L-NAME or 1400W. NO in liver was measured by electrochemical method after eNOS stimulation by calcium ionophore. Total NOS activity and nitrite/nitrate content were measured in liver and blood serum. The activity of free radical oxidation in liver was measured by chemiluminescent method. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were assayed in blood serum<br /><strong>Results.</strong> AA increased the activity of free radical processes in liver and markers of cytolysis in serum, as well as decreased eNOS and increased iNOS activities. L-NAME considerably inhibited eNOS and augmented the necrosogenic properties of AA, whereas 1400W partially prevented liver damage.<br /><strong>Conclusion.</strong> It has been concluded that in AA intoxication NO produced from eNOS is beneficial to the liver, but NO derived from the upregulated iNOS has deleterious effect.</p><p><strong>KEY WORDS:</strong> nitric oxide, toxic hepatitis, NOS inhibitors.<br /><br /></p>

The influence of humus acids of peloids and its components on free radical processes

2018 ◽  
Vol 64 (5) ◽  
pp. 429-432 ◽  
Author(s):  
N.P. Avvakumova ◽  
F.H. Kamilov ◽  
A.V. Zhdanova ◽  
I.A. Men’shikova ◽  
Yu.V. Zhernov ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Albino Rats ◽  
Redox Systems ◽  
Radical Oxidation ◽  
The Third ◽  
Glutathione Peroxidases ◽  
Humus Acids ◽  
Radical Processes

The effect of individual components of humic substances of peloid on free radical oxidation processes has been investigated under conditions of oxidative stress induced in albino rats. Biological activity of peloids was determined using such parameters as the general antioxidant activity, activity superoxide dismutases, catalases and glutathione peroxidases on the third and tenth day of the experiment. Results indicate that the state of oxidative stress can be corrected on the third day of the experiment. Humic acids restore not only normal physiological redox systems, but also increase the activity of antioxidant enzymes on the 10th day.

scholarly journals Features of free radical lipid peroxidation and serum proteins at dental implants and peri-implantitis

2015 ◽  
Vol 96 (5) ◽  
pp. 756-759 ◽  
Author(s):  
D V Plyukhin ◽  
V E Tseylikman ◽  
O B Tseylikman ◽  
A I Sinitskiy
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Blood Serum ◽  
Dental Implants ◽  
Schiff Bases ◽  
Free Radical Oxidation ◽  
Protein Carbonylation ◽  
Radical Oxidation ◽  
Lipid Peroxidation Products ◽  
Dental Implantation

Aim. Determine the most informative indicators of free radical peroxidation in blood plasma for predicting the complications of dental implantation. Methods. The study included 84 patients of both with dental implants, including 31 patients without any complications and 53 patients with peri-implantitis. The levels of lipid peroxidation products and protein carbonylation were measured in peripheral blood. Results. Moderate intensification of lipid peroxidation and protein carbonylation was discovered in blood serum of patients with dental implants. Increased free radical oxidation in the blood was not limited to lipid peroxidation and resulted in increased serum levels of carbonylated proteins. Facilitation of oxidative degradation of proteins in the blood was associated with decreased resistance to «carbonyl stress», manifested as increased level of Fe+2/H2O2-induced carbonylation. Development of peri-implantitis resulted in a greater enhancement of free radical oxidation of blood. Isopropanol-soluble and heptane-soluble Schiff bases level was increased in blood serum as well. Similar to patients with dental implants, increased levels of carbonylated proteins was observed in blood serum at peri-implantitis. However, free radical oxidation was more intense in patients with peri-implantitis compared to patients with dental implants. Indeed, the level of heptane-soluble Schiff bases at the group with peri-implantitis was 2 times higher as compared to dental implants group, and the level of isopropanol-soluble lipid peroxidation products in inflammatory complications after implantation has reached fourfold increase compared with patients who underwent operation without complications. Conclusion. Investigated indicators of free radical oxidation may be used as markers of peri-implantitis. Among them, the most informative indicators are Schiff bases and metal-catalyzed protein oxidation.

Metabolic state of perifocal zone and resection line of skin melanoma.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. e19049-e19049
Author(s):  
Elena Frantziyantz ◽  
Farkhad Dzhabarov ◽  
Liudmila Rosenko ◽  
Victoria Pozdnyakova ◽  
Ekaterina Komarova
Keyword(s):  
Free Radical ◽  
Catalase Activity ◽  
Concentration Level ◽  
Tumour Tissue ◽  
Skin Melanoma ◽  
Intact Skin ◽  
Free Radical Processes ◽  
Resection Line ◽  
Radical Processes ◽  
Vitamins E And A

e19049 Background: The state of metabolism in perifocal zone and morphologically intact skin along resection line is of importance for the processes of neoplasm metastasizing and relapse, including skin melanoma. Normalization of the state of free-radical processes, first of all along resection line, and then in neoplasm perifocal zone, should be a characteristic of pathogenically substantiated neoadjuvant influence. Methods: to study activity of free-radical processes in tissue of skin melanoma relapse, its perifocal zone (PZ) (1 cm from tumour edge) and in skin along resection line (3-5 cm from tumour edge) we investigated concentration of vitamins E and A, activity of superoxide dismutase (SOD), catalase, summary peroxidase activity (SPA), and malonic dialdehyde level (MDA) in patients with skin melanoma (pT2-3N1-2M0). The study group included 54 samples after treatment (chemotherapy and radiation). Control groups: 45 samples before treatment (CG1) and 27 samples of intact skin after plastic operations (CG2). Results: After treatment MDA concentration in tumour tissue increased by 33.4% in comparison with the CG1, but remained 17.7% lower than in CG2. Average increase in concentration of vitamins E and A was equal to 1.8 times, catalase activity decreased by 30.3% in comparison with parameters before treatment (p<0.05). SOD/catalase coefficient was 1.3 times higher than in the CG1, but 3.4 times lower than CG2.More manifested changes in activity of free-radical processes were observed in PZ: MDA concentration level after treatment increased by 30% in comparison with the CG1, and differed from the one in CG2 only by 10% (p<0.05). Catalase activity decreased by 1.5 times and didn’t significantly differ from the corresponding parameter in tumour tissue after treatment. Normalization of concentration level of vitamins A and E without change of their ratio was observed. There was 1.6-fold increase of SOD/SPA coefficient in comparison with the CG1, however it was 2.4 times lower than CG2 (p<0.05). All parameters under study along resection line after treatment didn’t significantly differ from parameters in CG2. Conclusions: The performed treatment completely restores broken metabolism in skin along resection line, partially–in tumour perifocal zone.

scholarly journals Peculiarities of lipid peroxidation processes in the body of cats with urinary tract diseases and their correction with commercial feeds

2021 ◽  
pp. 37-40
Author(s):  
Olga Andreevna Vorontsova ◽  
Nikolay Alexandrovich Pudovkin ◽  
Vladimir Vasilievich Salautin ◽  
Sergey Dmitrievich Klyukin
Keyword(s):  
Urinary Tract ◽  
Blood Serum ◽  
Catalase Activity ◽  
Free Radical Oxidation ◽  
The Body ◽  
Diene Conjugates ◽  
Radical Oxidation ◽  
Mda Content ◽  
Peroxidation Processes ◽  
Commercial Feeds

The article presents the results of studies on the effect of commercial food on the processes of free radical oxidation of lipids in the body of cats with urinary tract pathology. It has been found that the concentration of diene conjugates in the blood serum of sick animals increases significantly by 15.1 - 36.7% relative to healthy ones. The MDA content in the blood serum of healthy animals was 6.05 ± 0.28 nmol / g. In animals with urolithiasis, this indicator increased by about 2 times. When feeding with commercial food and homemade food, LPO processes normalize, which is most pronounced on the 21st day. The average activity of catalase in the blood serum of sick animals was increased from 30.6% to 40.2% relative to healthy ones (15.15 ± 0.61 ?mol / ml). After feeding with therapeutic and prophylactic fodders, by the 7th day, the catalase activity did not significantly decrease compared to the initial level, but remained higher than in control animals by 19.6% - 37.1%. On the 14th day, the catalase activity did not change significantly compared with the 7th day and was higher than the control values by 21.5% - 27.5%.

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