scholarly journals VARIABILITY OF FATTY ACID PROFILES, OXIDATIVE STABILITY AND NUTRITIVE QUALITY OF OIL IN SELECTED SOYBEAN GENOTYPES

Poljoprivreda ◽  
2020 ◽  
Vol 26 (2) ◽  
pp. 11-20
Author(s):  
Maja Matoša Kočar ◽  
◽  
Sonja Vila ◽  
Sonja Petrović ◽  
Andrijana Rebekić ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Oxidative Stability ◽  
Negative Relationship ◽  
Fatty Acid Profiles ◽  
Data Set ◽  
Positive Correlation ◽  
Eigen Analysis ◽  
Nutritive Quality ◽  
Soybean Genotypes

The purpose of this study (2010‒12) was to investigate the fatty acid profiles (palmitic – PA, stearic – SA, oleic – OA, linoleic – LA, linolenic – LNA, saturated fatty acids – SFA, monounsaturated fatty acids – MUFA, polyunsaturated fatty acids – PUFA), oxidative stability (MUFA/PUFA, OA/LA), and nutritive quality (LA/LNA) of oil produced by 13 early soybean genotypes created at the Agricultural Institute Osijek, Croatia. The analysis of variance determined significant effect of genotype (G), year (Y), and GxY interaction on all examined traits. The correlation analysis indicated the most important positive correlation existed between the SA and OA, SA and LNA, and LA and LNA, and the most important negative relationship between OA and LA, and OA and LNA. The principal component analysis yielded four significant principal components (PCs) with the eigenvalues > 1, which together accounted for 57.84% of the total variability in the data set. The Eigen analysis of the correlation matrix loadings indicated MUFA/PUFA, MUFA, OA/LA, and OA mostly contribute to the PC1 axis and are in a positive correlation with it, while PUFA, LA, and LNA mostly contribute to a negative direction. These results will facilitate the planning of future breeding programs, aimed at adjusting the fatty acid profiles according to the needs of the processing industry and end consumers.

Evaluating the Use of Fatty Acid Profiles to Differentiate Human Pathogenic and Nonpathogenic Listeria Species

2012 ◽  
Vol 95 (5) ◽  
pp. 1457-1459 ◽  
Author(s):  
Paul Whittaker
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Methyl Esters ◽  
Brain Heart Infusion ◽  
Methyl Tert Butyl Ether ◽  
Human Pathogen ◽  
Fatty Acid Profiles ◽  
Butyl Ether ◽  
Data Set ◽  
Listeria Species

Abstract Listeria monocytogenes is a Gram-positive human pathogen that is responsible for serious infections in immunocompromised individuals and pregnant women. Because of recent epidemics caused by food contaminated with L. monocytogenes, rapid methods for the detection of this pathogen in food are of interest. Capillary gas chromatography with flame ionization detection (GC-FID) was used to determine the cellular fatty acid profiles of six species of Listeria. The six different species are L. monocytogenes, L. ivanovii, L. innocua, L. welshimeri, L. seeligeri, and L. grayi. For GC-FID analysis, whole cell fatty acid methyl esters (FAMEs) from cells cultured on brain heart infusion (BHI) agar at 35 ° C for 24 h were obtained by saponification, methylation, and extraction into hexane/methyl tert-butyl ether. A preliminary data set for 15 strains of Listeria species was prepared using fatty acid profiles from two or three replicates prepared on different days. Major fatty acids of the Listeria strains evaluated in this study were C15:0 iso, C15:0 ante iso, C16:0 iso, C16:0, C17:0 iso, and C17:0 ante iso. All of the major fatty acids differ significantly among these six species. The two fatty acids C17:0 ante iso and C15:0 ante iso showed the highest percentages, and the ratio of the two clearly showed significant differences between the human pathogen L. monocytogenes and the five nonpathogenic species. Analysis of FAMEs from Listeria strains grown on BHI agar by a GC-FID method is a sensitive procedure for identification of these organisms and differentiation between pathogenic and nonpathogenic species.

scholarly journals Effect of Sunflower, Linseed and Soybean Meal in Pig Diet on Chemical Composition, Fatty Acid Profile of Meat and Backfat, and Its Oxidative Stability

2016 ◽  
Vol 66 (3) ◽  
pp. 359-372 ◽  
Author(s):  
Vesna Đorđević ◽  
Jasna Đorđević ◽  
Milan Baltić Ž. ◽  
Milica Laudanović ◽  
Vlado Teodorović ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Chemical Composition ◽  
Oxidative Stability ◽  
Soybean Meal ◽  
Monounsaturated Fatty Acids ◽  
The Other ◽  
Fatty Acid Profiles ◽  
Pufa Content ◽  
Feeding Study

AbstractThe aim of this study was to examine the effect of added sunflower, linseed or soybean meal to a standard pig fattening diet on the chemical composition, fatty acid profiles of meat and backfat, and on the oxidative stability of backfat from pigs. The content of saturated (SFA) and monounsaturated fatty acids (MUFA) was significantly lower (P<0.01), while content of polyunsaturated fatty acids (PUFA) was significantly higher (P<0.01) in the diet with added linseed. The feeding study was conducted on 30 pigs, with groups of 10 pigs fed one of the three different diets for 46 days before slaughter. There were no differences in the chemical composition (protein, water, fat, minerals) of meat from pigs fed the different diets. The content of SFA and MUFA was significantly higher, while the average PUFA content was significantly lower (P<0.01) in meat and backfat of pigs fed diet with added linseed. The content of n-6 fatty acids was significantly lower and n-3 fatty acids significantly higher, with more desirable n-6/n-3 ratio, in meat and backfat of pigs fed diet with added linseed. Malondialdehyde in the backfat of pigs fed diet with added linseed was significantly lower than that in the other two diet groups after the tissue was stored frozen at −20°C for 3, 6, 9 and 12 months.

scholarly journals Evaluating the Use of Fatty Acid Profiles to Identify Francisella tularensis

2007 ◽  
Vol 90 (2) ◽  
pp. 465-469 ◽  
Author(s):  
Paul Whittaker ◽  
James B Day ◽  
Sherill K Curtis ◽  
Frederick S Fry
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Francisella Tularensis ◽  
Methyl Esters ◽  
Saturated Fatty Acids ◽  
Principal Component ◽  
Cellular Fatty Acid ◽  
Fatty Acid Profiles ◽  
Data Set ◽  
Mueller Hinton

Abstract Rapid capillary gas chromatography (GC) with flame-ionization detection was used to determine the cellular fatty acid profiles of Francisella tularensis. Two subspecies of F. tularensis, the live vaccine strain (LVS) derived from holarctica and a novicida strain Utah 112 (U112), were used to compare the extracted fatty acid methyl esters (FAMEs). A data set for the 2 subspecies was prepared using fatty acid profiles of bacteria grown on 2 types of media, Mueller-Hinton and cysteine heart agar supplemented with 5% rabbit blood (CHAB), and harvested at various time intervals (Day 1 through Day 4) with replicates prepared on different days. A total of 204 samples were analyzed. The results showed that these fatty acid quantitative profiles were unique for each of the subspecies and could be used as a fingerprint for the organism. It was determined by this rapid method that approximately 88% of the fatty acids in both the LVS and U112 strains included 6 saturated fatty acids: 10:0, 12:0, 14:0, 16:0, 18:0, and 20:0; and 4 hydroxy fatty acids 10:0 2OH, 16:0 3OH, 17:0 3OH, and 18:0 3OH. Data analysis and determination of clustering were performed by principal component analysis (PCA) and soft independent modeling of class analogy (SIMCA). Both PCA and SIMCA showed clear separation of the LVS and U112 strain and would be useful for prediction of unknowns. It was determined that the incubation time can be reduced from 48 to 24 h, and results are highly predictive for the identification of F. tularensis. In summary, analysis of FAMEs from F. tularensis subspecies LVS and U112 grown on CHAB or Mueller-Hinton media, and using a rapid GC method can provide a sensitive procedure for identification of these organisms.

scholarly journals Fatty acid profiles of muscle, liver, heart and kidney of Australian prime lambs fed different polyunsaturated fatty acids enriched pellets in a feedlot system

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hung Van Le ◽  
Don Viet Nguyen ◽  
Quang Vu Nguyen ◽  
Bunmi Sherifat Malau-Aduli ◽  
Peter David Nichols ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Fatty Acid Profiles

scholarly journals PSXI-32 Effects of algae DHA on fatty acid profile of plasma red blood cell membrane and fecal microbiota of adult cats

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 319-319
Author(s):  
Carrie James ◽  
Sandra L Rodriguez-Zas ◽  
Maria R C de Godoy
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Blood Cell ◽  
Fish Oil ◽  
Red Blood Cell ◽  
Fecal Microbiota ◽  
Fatty Acid Profiles ◽  
Male Adult ◽  
Poultry Fat ◽  
Adult Cats

Abstract There is evidence that algae can be a sustainable alternative of omega-3 polyunsaturated fatty acids (w-3 PUFA; DHA and EPA) in the diets of felines, but more information is needed to determine bioavailability of algal w-3 PUFAs in felines. Therefore, the objective of this study was to determine the effects of dietary supplementation of algae DHA on plasma and red blood cell (RBC) membrane fatty acid profiles and fecal microbiota of adult cats. A complete randomized design was utilized with thirty female and male adult cats (mean age: 1.8 ± 0.03 yr, mean BW: 4.5 ± 0.8 kg) which were fed an assigned diet for 90 d. Three diets were formulated with poultry fat alone or inclusion of 2% fish oil or 2% algae DHA meal. Blood samples were collected after fasting on 0, 30, 60 and 90 d to be analyzed for plasma and red blood cell fatty acid profiles. A fresh fecal sample was collected within 15 min of defecation from each cat to be analyzed for fecal microbiota. Illumina 16S rRNA sequencing from V4 region was completed using MiSeq and analyzed using QIIME 2. Plasma and RBC fatty acid concentrations at baseline were similar among all cats and treatment groups. However, dietary treatment had a significant effect on the concentrations of several fatty acids in plasma and RBC over time. Plasma and RBC concentrations of DHA were greater (P &lt; 0.05) for cats fed the algal DHA diet compared to the control and fish oil diets. Conversely, plasma and RBC concentrations of EPA did not differ among treatments when analyzed as a change from baseline. Beta- and alpha-diversity did not differ among treatments, indicating that 2% fish oil or algal-DHA meal does alter fecal microbiota of cats in contrast with cats fed a poultry fat-based diet.

scholarly journals Modeling the effect of heat treatment on fatty acid composition in home-made olive oil preparations

2020 ◽  
Vol 15 (1) ◽  
pp. 606-618 ◽  
Author(s):  
Dani Dordevic ◽  
Ivan Kushkevych ◽  
Simona Jancikova ◽  
Sanja Cavar Zeljkovic ◽  
Michal Zdarsky ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Olive Oil ◽  
Heating Temperature ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Fatty Acid Profiles ◽  
Refined Olive Oil ◽  
Cross Correlation Analysis

AbstractThe aim of this study was to simulate olive oil use and to monitor changes in the profile of fatty acids in home-made preparations using olive oil, which involve repeated heat treatment cycles. The material used in the experiment consisted of extra virgin and refined olive oil samples. Fatty acid profiles of olive oil samples were monitored after each heating cycle (10 min). The outcomes showed that cycles of heat treatment cause significant (p < 0.05) differences in the fatty acid profile of olive oil. A similar trend of differences (p < 0.05) was found between fatty acid profiles in extra virgin and refined olive oils. As expected, the main differences occurred in monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs). Cross-correlation analysis also showed differences between the fatty acid profiles. The most prolific changes were observed between the control samples and the heated (at 180°C) samples of refined olive oil in PUFAs, though a heating temperature of 220°C resulted in similar decrease in MUFAs and PUFAs, in both extra virgin and refined olive oil samples. The study showed differences in fatty acid profiles that can occur during the culinary heating of olive oil. Furthermore, the study indicated that culinary heating of extra virgin olive oil produced results similar to those of the refined olive oil heating at a lower temperature below 180°C.

scholarly journals Fatty Acid Composition of Cosmetic Argan Oil: Provenience and Authenticity Criteria

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4080
Author(s):  
Milena Bučar Miklavčič ◽  
Fouad Taous ◽  
Vasilij Valenčič ◽  
Tibari Elghali ◽  
Maja Podgornik ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Discriminant Analysis ◽  
Trans Fatty Acids ◽  
Correct Classification ◽  
Fatty Acid Profiles ◽  
Correct Classification Rate ◽  
Classification Rate ◽  
Argan Oil ◽  
Geographical Regions

In this work, fatty-acid profiles, including trans fatty acids, in combination with chemometric tools, were applied as a determinant of purity (i.e., adulteration) and provenance (i.e., geographical origin) of cosmetic grade argan oil collected from different regions of Morocco in 2017. The fatty acid profiles obtained by gas chromatography (GC) showed that oleic acid (C18:1) is the most abundant fatty acid, followed by linoleic acid (C18:2) and palmitic acid (C16:0). The content of trans-oleic and trans-linoleic isomers was between 0.02% and 0.03%, while trans-linolenic isomers were between 0.06% and 0.09%. Discriminant analysis (DA) and orthogonal projection to latent structure—discriminant analysis (OPLS-DA) were performed to discriminate between argan oils from Essaouira, Taroudant, Tiznit, Chtouka-Aït Baha and Sidi Ifni. The correct classification rate was highest for argan oil from the Chtouka-Aït Baha province (90.0%) and the lowest for oils from the Sidi Ifni province (14.3%), with an overall correct classification rate of 51.6%. Pairwise comparison using OPLS-DA could predictably differentiate (≥0.92) between the geographical regions with the levels of stearic (C18:0) and arachidic (C20:0) fatty acids accounting for most of the variance. This study shows the feasibility of implementing authenticity criteria for argan oils by including limit values for trans-fatty acids and the ability to discern provenance using fatty acid profiling.

scholarly journals Effects of polyunsaturated fatty acids in diets fed to sows on fatty acids in brain, muscle and skin of their piglets

2020 ◽  
Vol 50 (1) ◽  
pp. 47-54
Author(s):  
I De Gasperín ◽  
J.G. Vicente ◽  
J.M. Pinos-Rodríguez ◽  
F Montiel ◽  
R Loeza ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Soybean Oil ◽  
Fatty Acid Profile ◽  
Saturated Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Fatty Acid Profiles ◽  
Polyunsaturated Fat ◽  
Pork Lard ◽  
Piglet Survival

The aim of this research was to determine fatty acid profiles in piglet brain, skin, and muscle, and in the milk of sows fed fat with different saturation grades during gestation and lactation. At 42 days of gestation, 50 multiparous sows were randomly allocated to one of two treatments, namely a diet containing pork lard (n = 25) and a diet containing soybean oil (n = 25). The fats were provided at 3.6% during gestation and at 4% during lactation. The experimental diets were offered through the weaning of the piglets. The fatty acid profile of the milk was determined fourteen days after parturition. At weaning (21 days postpartum) and seven days later, one of the piglets (n = 64) from 16 sows allocated to each treatment was selected at random to determine fatty acid profiles in brain, skin and muscle. Saturated and monounsaturated fatty acids were higher in the diet with pork lard than in that with soybean oil, in which the polyunsaturated fat content was higher. A higher saturation of fatty acids was found in milk from the sows that consumed pork lard, which contained more saturated fatty acids than the milk from sows that consumed soybean oil. The fatty acid profiles in muscle and skin of the piglets were affected by the diet of the sows. However, the fatty acid profile of the piglets’ brains was not affected by the diet of their mothers. Keywords: fat saturation, lard, piglet survival, sow feeding, soybean oil

scholarly journals Monitoring of Biochemical Compounds and Fatty Acid in Marine Microalgae from the East Coast of Thailand

2018 ◽  
Vol 17 (4) ◽  
pp. 334-347
Author(s):  
Kwanchayanawish MACHANA ◽  
Amonrat KANOKRUNG ◽  
Sirinart SRICHAN ◽  
Boonyadist VONGSAK ◽  
Maliwan KUTAKO ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
East Coast ◽  
Predictive Accuracy ◽  
Partial Least Square ◽  
Least Square ◽  
Biodiesel Production ◽  
Fatty Acid Profiles ◽  
Omega 3 ◽  
Ft Ir

Determinations of fatty acid profiles of five microalgae; Amphora sp., Chaetoceros sp., Melosira sp., Bellerochae sp., and Lithodesmium sp., from the east coast of Thailand were evaluated by conventional Gas Chromatography-Flame Ionization Detector (GC-FID). The results exhibited that the fatty acids suitable for biodiesel production were the most frequent entities encountered in all microalgae profiles. The GC chromatogram of fatty acid profiles in microalgae showed that both Amphora sp. and Chaetoceros sp. comprised essential omega-3 fatty acids, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Additionally, this study assessed whether Fourier Transform infrared (FT-IR) microspectroscopy could be used to evaluate and monitor the biochemical compositions of microalgae, including lipid, carbohydrate, and protein profiles, by using colorimetric methods. Results showed that FT-IR spectra combined with biochemical values of lipid, carbohydrate, and protein contents were used as predictive models generated by partial least square (PLS) regression. Cross-validation of the lipid, protein, and carbohydrate models showed high degrees of statistical accuracy with RMSECV values of approximately 0.5 - 3.22 %, and a coefficient of regression between the actual and predicted values of lipids, carbohydrates, and proteins were 92.66, 95.73, and 96.43 %, respectively. The RPD values were all high (> 3), indicating good predictive accuracy. This study suggested that FT-IR could be a tool for the simultaneous measurement of microalgae composition of biochemical contents in microalgae cells.

scholarly journals Characterization of planktonic and biofilm fatty acid profiles and evaluation of their trophic transfer potential to Hyalella Azteca

2021 ◽  
Author(s):  
Jerry Chien-Yao Chao
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Bacterial Species ◽  
Saturated Fatty Acids ◽  
Hyalella Azteca ◽  
Fatty Acid Profiles ◽  
Fa Composition ◽  
Branched Chain ◽  
Transfer Potential

Fatty acid (FA) composition between biofilms and batch planktonic cultures were compared for two bacterial species Pseudomonas aeruginosa and Staphylococcus aureaus. Biofilm cultures exhibited decrease in saturated fatty acids (SAFA) that potentially conform to a more fluidic biophysical membrane property. The amount of FA in the biofilms' extracellular polymeric substance was not sufficient to consider it having a major contribution to the observed differences between biofilms and batch planktonic cultures. While biofilm grazing by the amphipod Hyalella azteca was evident, only certain bacteria-specific FA appeared to have the potential to be retained (odd-number SAFA and branched-chain FA). H. azteca with diet strictly consisted of bacteria biofilms did not demonstrate significant changes in their nutritional condition in terms of ω-3 and ω-6 polyunsaturated fatty acids (PUFA): combined with the results from fasting trials, H. azteca appears to have the capacity to retain ω-3 and ω-6 PUFAs up to 10 days.

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