Modificaciones metabólicas y estructurales de la pared celular asociadas a la habituación incipiente de suspensiones celulares de maı́z a diclobenil = Structural and metabolic cell wall modifications related to the short‐term habituation of maize cell suspensions to dichlobenil

2014 ◽  
Author(s):  
María de Castro Rodríguez
Keyword(s):  
Cell Wall ◽  
Cell Suspensions ◽  
Short Term ◽  
Maize Cell

scholarly journals Short-term control of maize cell and root water permeability through plasma membrane aquaporin isoforms

2011 ◽  
Vol 35 (1) ◽  
pp. 185-198 ◽  
Author(s):  
CHARLES HACHEZ ◽  
DMITRY VESELOV ◽  
QING YE ◽  
HAGEN REINHARDT ◽  
THORSTEN KNIPFER ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Water Permeability ◽  
Short Term ◽  
Maize Cell

Effect of Short-Term Ozone Treatments on Tomato (Solanum lycopersicumL.) Fruit Quality and Cell Wall Degradation

2010 ◽  
Vol 58 (1) ◽  
pp. 594-599 ◽  
Author(s):  
Luis Rodoni ◽  
Natalia Casadei ◽  
Analía Concellón ◽  
Alicia R. Chaves Alicia ◽  
Ariel R. Vicente
Keyword(s):  
Cell Wall ◽  
Fruit Quality ◽  
Cell Wall Degradation ◽  
Short Term ◽  
Ozone Treatments

Métabolisme de l'acide indolyl-3-acétique dans les cultures de cellules d'Acer pseudoplatanus cultivés in vitro

1977 ◽  
Vol 55 (19) ◽  
pp. 2530-2534 ◽  
Author(s):  
F. Maillard ◽  
J.-P. Zrÿd
Keyword(s):  
Cell Wall ◽  
Acetic Acid ◽  
Culture Medium ◽  
Degradation Products ◽  
Cell Suspensions ◽  
The Other ◽  
Acer Pseudoplatanus ◽  
Kinetics Of

Incubation of cell suspensions of sycamore (Acer pseudoplatanus) with β-indoyl-3-acetic acid (IAA) first led to the formation of IAA-glycosides, then to that of IAA-aspartate. Great differences are observed between the kinetics of IAA transformed by two distinct strains: one, auxin dependent (S), the other, auxin independent (MB). Other degradation products are only found in the culture medium. The localization of IAA-degrading systems in the cell wall is postulated. The auxin requirement of the S strain is discussed.

Distribution within the plant or compartmentation does not contribute substantially to the detoxification of excess boron in sunflower (Helianthus annuus)

1999 ◽  
Vol 26 (2) ◽  
pp. 95 ◽  
Author(s):  
Frank Dannel ◽  
Heidrun Pfeffer ◽  
Volker Römheld
Keyword(s):  
Cell Wall ◽  
Helianthus Annuus ◽  
Term Treatment ◽  
Substantial Contribution ◽  
Insoluble Residue ◽  
Tolerance Mechanism ◽  
Additional Experiment ◽  
Short Term ◽  
Short Term Treatment ◽  
Helianthus Annuus L

Distribution and compartmentation of boron as possible mechanisms for the notable tolerance of sunflower (Helianthus annuus L.) to excess B supply have been studied. Following a short- term treatment (6 h) with 1000 µМ B, the distribution of B between different shoot organs was preferentially directed to the readily transpiring ones. Thus, B distribution in sunflower plants seems to be mainly influenced by transpiration and does not act as a B tolerance mechanism in sunflower. As a second possible mechanism for B tolerance, the compartmentation of B between water insoluble residue (i.e. cell wall) and cell sap (i.e. symplasm) was determined. Additional binding of B in the cell wall in response to excess B supply for 6 h was very low in leaves and negligible in roots. Thus, a substantial contribution of the cell wall in detoxification of excess B can be ruled out. In an additional experiment with a B supply of 1 µМ (marginal) to 1000 µМ (moderately toxic), the compartmentation of B between the apoplasmic fluid and the cell sap was investigated. The B concentration in the apoplasmic fluid was always lower than that in the symplasm. Thus, B was not excluded from the symplasm, but accumulated within it. The apoplasmic fluid did not contribute substantially to the detoxification of excess B in the leaf.

scholarly journals Short-Term Boron Deprivation Inhibits Endocytosis of Cell Wall Pectins in Meristematic Cells of Maize and Wheat Root Apices

2002 ◽  
Vol 130 (1) ◽  
pp. 415-421 ◽  
Author(s):  
Qin Yu ◽  
Andrej Hlavacka ◽  
Toru Matoh ◽  
Dieter Volkmann ◽  
Diedrik Menzel ◽  
...  
Keyword(s):  
Cell Wall ◽  
Wheat Root ◽  
Short Term ◽  
Meristematic Cells ◽  
Root Apices

scholarly journals Short-term radiorespirometry of cell suspensions

1974 ◽  
Vol 144 (3) ◽  
pp. 487-496 ◽  
Author(s):  
W G Duncombe
Keyword(s):  
Detailed Analysis ◽  
Glucose Oxidation ◽  
Specific Radioactivity ◽  
Cell Types ◽  
Cell Suspensions ◽  
Short Term ◽  
Low Concentration ◽  
High Specific Radioactivity

1. An apparatus and method are described with which the oxidation of labelled substrates to14CO2 by cell suspensions may be examined. 2. The use of high-specific-radioactivity substrates at low concentration together with frequent quantitative collection of CO2 permit a more detailed analysis of the appearance of various substrate carbon atoms as CO2 than is possible by existing techniques. 3. Typical experiments with various cell types are reported, in which pathways of glucose oxidation are examined.

scholarly journals Overexpression of a Sugarcane BAHD Acyltransferase Alters Hydroxycinnamate Content in Maize Cell Wall

2021 ◽  
Vol 12 ◽  
Author(s):  
Amanda Fanelli ◽  
David M. Rancour ◽  
Michael Sullivan ◽  
Steven D. Karlen ◽  
John Ralph ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Expression Patterns ◽  
Fold Increase ◽  
Coumaric Acid ◽  
Maize Cell ◽  
High Conservation ◽  
Mild Hydrolysis ◽  
Similar Gene ◽  
Bahd Acyltransferase

The purification of hydroxycinnamic acids [p-coumaric acid (pCA) and ferulic acid (FA)] from grass cell walls requires high-cost processes. Feedstocks with increased levels of one hydroxycinnamate in preference to the other are therefore highly desirable. We identified and conducted expression analysis for nine BAHD acyltransferase ScAts genes from sugarcane. The high conservation of AT10 proteins, together with their similar gene expression patterns, supported a similar role in distinct grasses. Overexpression of ScAT10 in maize resulted in up to 75% increase in total pCA content. Mild hydrolysis and derivatization followed by reductive cleavage (DFRC) analysis showed that pCA increase was restricted to the hemicellulosic portion of the cell wall. Furthermore, total FA content was reduced up to 88%, resulting in a 10-fold increase in the pCA/FA ratio. Thus, we functionally characterized a sugarcane gene involved in pCA content on hemicelluloses and generated a C4 plant that is promising for valorizing pCA production in biorefineries.

scholarly journals Lysozyme and Penicillin Inhibit the Growth of Anaerobic Ammonium-Oxidizing Planctomycetes

2013 ◽  
Vol 79 (24) ◽  
pp. 7763-7769 ◽  
Author(s):  
Ziye Hu ◽  
Theo van Alen ◽  
Mike S. M. Jetten ◽  
Boran Kartal
Keyword(s):  
Cell Wall ◽  
Continuous Cultivation ◽  
Penicillin G ◽  
Anammox Bacteria ◽  
Short Term ◽  
Content Type ◽  
Genome Data ◽  
Wall Structures ◽  
Anammox Activity

ABSTRACTAnaerobic ammonium-oxidizing (anammox) planctomycetes oxidize ammonium in the absence of molecular oxygen with nitrite as the electron acceptor. Although planctomycetes are generally assumed to lack peptidoglycan in their cell walls, recent genome data imply that the anammox bacteria have the genes necessary to synthesize peptidoglycan-like cell wall structures. In this study, we investigated the effects of two antibacterial agents that target the integrity and synthesis of peptidoglycan (lysozyme and penicillin G) on the anammox bacteriumKuenenia stuttgartiensis. The effects of these compounds were determined in both short-term batch incubations and long-term (continuous-cultivation) growth experiments in membrane bioreactors. Lysozyme at 1 g/liter (20 mM EDTA) lysed anammox cells in less than 60 min, whereas penicillin G did not have any observable short-term effects on anammox activity. Penicillin G (0.5, 1, and 5 g/liter) reversibly inhibited the growth of anammox bacteria in continuous-culture experiments. Furthermore, transcriptome analyses of the penicillin G-treated reactor and the control reactor revealed that penicillin G treatment resulted in a 10-fold decrease in the ribosome levels of the cells. One of the cell division proteins (Kustd1438) was downregulated 25-fold. Our results suggested that anammox bacteria contain peptidoglycan-like components in their cell wall that can be targeted by lysozyme and penicillin G-sensitive proteins were involved in their synthesis. Finally, we showed that a continuous membrane reactor system with free-living planktonic cells was a very powerful tool to study the physiology of slow-growing microorganisms under physiological conditions.

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