scholarly journals Proteomics and secretomics of Penicillium chrysogenum: Molecular characterization of proteins relevant for penicillin biosynthesis = Proteómica y secretómica de "Penicillium chrysogenum": caracterización molecular de proteínas importantes para la biosíntesis de penicilina

2010 ◽  
Author(s):  
Mohammad Saeid Jami
Keyword(s):  
Molecular Characterization ◽  
Penicillium Chrysogenum ◽  
Penicillin Biosynthesis

Molecular characterization of the PR-toxin gene cluster in Penicillium roqueforti and Penicillium chrysogenum: Cross talk of secondary metabolite pathways

2014 ◽  
Vol 62 ◽  
pp. 11-24 ◽  
Author(s):  
Pedro I. Hidalgo ◽  
Ricardo V. Ullán ◽  
Silvia M. Albillos ◽  
Olimpio Montero ◽  
María Ángeles Fernández-Bodega ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Molecular Characterization ◽  
Secondary Metabolite ◽  
Cross Talk ◽  
Penicillium Chrysogenum ◽  
Toxin Gene ◽  
Penicillium Roqueforti

scholarly journals Molecular characterization of Penicillium chrysogenum virus: reconsideration of the taxonomy of the genus Chrysovirus

2004 ◽  
Vol 85 (7) ◽  
pp. 2111-2121 ◽  
Author(s):  
Daohong Jiang ◽  
Said A. Ghabrial
Keyword(s):  
Molecular Characterization ◽  
Penicillium Chrysogenum ◽  
Sequence Similarity ◽  
Minor Component ◽  
Amino Acid Sequences ◽  
Nucleotide Sequencing ◽  
Dsrna Segment ◽  
Cdna Clones

Molecular cloning and complete nucleotide sequencing of Penicillium chrysogenum virus (PcV) dsRNAs indicated that PcV virions contained four dsRNA segments with sizes of 3562, 3200, 2976 and 2902 bp. Each dsRNA segment had unique sequences and contained a single large open reading frame (ORF). In vitro translation of transcripts derived from full-length cDNA clones of PcV dsRNAs yielded single products of sizes similar to those predicted from the deduced amino acid sequences of the individual ORFs. Sequence similarity searches revealed that dsRNA1 encodes a putative RNA-dependent RNA polymerase. In this study, it was determined that dsRNA2 encodes the major capsid protein and that p4, encoded by dsRNA4, is virion-associated as a minor component. All four dsRNAs of PcV, like the genomic segments of viruses with multipartite genomes, were found to have extended regions of highly conserved terminal sequences at both ends. In addition to the strictly conserved 5′-terminal 10 nt, a second region consisting of reiteration of the sequence CAA was found immediately upstream of the AUG initiator codon. These (CAA) n repeats are reminiscent of the translational enhancer elements of tobamoviruses. The 3′-terminal 14 nt were also strictly conserved. As PcV and related viruses with four dsRNA segments (genus Chrysovirus) have not been previously characterized at the molecular level, they were provisionally classified in the family Partitiviridae, comprising viruses with bipartite genomes. This study represents the first report on molecular characterization of a chrysovirus and the results suggest the creation of a new family of mycoviruses with multipartite dsRNA genomes to accommodate PcV and related viruses.

scholarly journals Molecular characterization of a fungal gene paralogue of the penicillin penDE gene of Penicillium chrysogenum

2009 ◽  
Vol 9 (1) ◽  
pp. 104 ◽  
Author(s):  
Carlos García-Estrada ◽  
Inmaculada Vaca ◽  
Ricardo V Ullán ◽  
Marco A van den Berg ◽  
Roel AL Bovenberg ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Penicillium Chrysogenum ◽  
Pende Gene ◽  
Fungal Gene

scholarly journals Characterization of an Autoinducer of Penicillin Biosynthesis in Penicillium chrysogenum

2011 ◽  
Vol 77 (16) ◽  
pp. 5688-5696 ◽  
Author(s):  
Jorge Martín ◽  
Carlos García-Estrada ◽  
Ángel Rumbero ◽  
Eliseo Recio ◽  
Silvia M. Albillos ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Penicillium Chrysogenum ◽  
Molecular Mechanisms ◽  
Biological Activities ◽  
Northern Analysis ◽  
Penicillin Biosynthesis ◽  
Control Of Gene Expression ◽  
Content Type ◽  
Direct Testing

ABSTRACTFilamentous fungi produce an impressive variety of secondary metabolites; many of them have important biological activities. The biosynthesis of these secondary metabolites is frequently induced by plant-derived external elicitors and appears to also be regulated by internal inducers, which may work in a way similar to that of bacterial autoinducers. The biosynthesis of penicillin inPenicillium chrysogenumis an excellent model for studying the molecular mechanisms of control of gene expression due to a good knowledge of the biochemistry and molecular genetics of β-lactam antibiotics and to the availability of its genome sequence and proteome. In this work, we first developed a plate bioassay that allows direct testing of inducers of penicillin biosynthesis using single colonies ofP. chrysogenum. Using this bioassay, we have found an inducer substance in the conditioned culture broths ofP. chrysogenumandAcremonium chrysogenum. No inducing effect was exerted by γ-butyrolactones, jasmonic acid, or the penicillin precursor δ-(l-α-aminoadipyl)-l-cysteinyl-d-valine. The conditioned broth induced penicillin biosynthesis and transcription of thepcbAB,pcbC, andpenDEgenes when added at inoculation time, but its effect was smaller if added at 12 h and it had no effect when added at 24 h, as shown by Northern analysis andlacZreporter studies. The inducer molecule was purified and identified by mass spectrometry (MS) and nuclear magnetic resonance (NMR) as 1,3-diaminopropane. Addition of pure 1,3-diaminopropane stimulated the production of penicillin by about 100% compared to results for the control cultures. Genes for the biosynthesis of 1,3-diaminopropane have been identified in theP. chrysogenumgenome.

scholarly journals Molecular characterization of a Penicillium chrysogenum exo-1,5-α-L -arabinanase that is structurally distinct from other arabinan-degrading enzymes

FEBS Letters ◽  
2004 ◽  
Vol 560 (1-3) ◽  
pp. 199-204 ◽  
Author(s):  
Tatsuji Sakamoto ◽  
Hideshi Ihara ◽  
Asako Shibano ◽  
Naoya Kasai ◽  
Hiroshi Inui ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Penicillium Chrysogenum ◽  
Degrading Enzymes

scholarly journals Molecular characterization of three loss-of-function mutations in the isopenicillin N-acyltransferase gene (penDE) of Penicillium chrysogenum.

1994 ◽  
Vol 176 (16) ◽  
pp. 4941-4948 ◽  
Author(s):  
F J Fernández ◽  
S Gutierrez ◽  
J Velasco ◽  
E Montenegro ◽  
A T Marcos ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Penicillium Chrysogenum ◽  
Loss Of Function ◽  
Isopenicillin N

1446: Molecular Characterization of Cyclooxygenase-2 in a Stimulated Prostate Stroma Cells

2006 ◽  
Vol 175 (4S) ◽  
pp. 467-467
Author(s):  
Victor K. Lin ◽  
Shih-Ya Wang ◽  
Claus G. Roehrbom
Keyword(s):  
Molecular Characterization ◽  
Cyclooxygenase 2 ◽  
Prostate Stroma
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