Analysis of the Protective Effect of Interleukin-5 On Sepsis Mice

2021 ◽  
Vol 7 (5) ◽  
pp. 1638-1645
Author(s):  
Xiaokun Wang ◽  
Fuyang Pei ◽  
Xiaoping Song ◽  
Hongmei Wang
Keyword(s):  
B Cells ◽  
Protective Effect ◽  
Intervention Group ◽  
Protective Role ◽  
Inflammatory Factors ◽  
Control Group ◽  
Response Factors ◽  
Interleukin 5 ◽  
Proliferation And Differentiation ◽  
Inflammatory Macrophages

IL-5 can stimulate the growth and differentiation of EOS, inhibit its apoptosis and stimulate the proliferation and differentiation of B cells with other cytokines, and act on mature B cells to promote the synthesis of IgA and IgM. Therefore, this paper takes C57BL / 6 mice as the experimental object to explore the protective effect of interleukin-5 on sepsis mice. The experimental results show that the serum cxcl-1 in sepsis model group is significantly higher than that in control group at each time point, reaching the peak at 12 h, while cxcl-1 in IL-5 intervention group is significantly lower than that in control group. IL-5 can reverse the liver injury induced by CLP in septic mice, the mechanism may be to reduce the production of inflammatory factors in the liver, and the liver function of mice was not damaged 12 hours after sepsis. IL-4 and IL-13 of type 2 inflammatory response factors can promote the conversion of macrophages to anti-inflammatory macrophages, inhibit the inflammatory reaction, and play a protective role in sepsis.

scholarly journals Protective Effects and Mechanisms of Rosuvastatin on Acute Kidney Injury Induced by Contrast Media in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Zehui Jiang ◽  
Jun Zhang ◽  
Yuanan Lu
Keyword(s):  
Protective Effect ◽  
Contrast Medium ◽  
Renal Injury ◽  
Intervention Group ◽  
Inflammatory Factors ◽  
Control Group ◽  
Renal Tubules ◽  
Protective Effects ◽  
Sod Activity ◽  
Biochemical Indicators

Objective. To explore the protective effect and mechanism of rosuvastatin on acute renal injury induced by a nonionic hypotonic contrast medium in rats. Methods. Forty-eight healthy adult SD rats were randomly divided into three groups: normal control group (NC); contrast medium control group (CM); and rosuvastatin intervention group (RI). The RI group was intragastrically administered with a 10 mg/kg of rosuvastatin 12 h prior to the contrast exposure. All rats in CM and RI groups were inoculated with 10 mL/kg of chemical (IV) while the same volume of saline for the NC group. At 24 h and 72 h posttreatments, pathomorphological changes of renal tubules were documented, respectively, and several biochemical indicators were tested to assess renal injury of experimental rats. Results. Compared with the CM group, rats in the RI group showed significantly reduced injury of kidneys and decreased levels of biochemical indicators such as blood Scr, blood Cys-C, urine NAG, urine α1-MG, and urine mALB. The serum Hs-CRP in the CM group increased significantly from 24 h to 72 h (p<0.05), but this was not observed in the rats of the RI group. In addition, SOD activity in the RI group was significantly increased (p<0.01) while SOD activity in renal tissue decreased significantly with time in the CM group (p<0.05). Conclusion. Short-term intervention with rosuvastatin can lead to reduced kidney damage associated with the contrast agent by reducing the levels of inflammatory factors and oxidative stress. Thus, rosuvastatin intervention has a protective effect on rats from contrast-induced nephropathy.

A Randomized Controlled Study of the Effectiveness of Electrophysiological Monitoring of Dexmedetomidine in Patients with Brain Damage of Various Origins

2020 ◽  
Vol 75 (5) ◽  
pp. 490-499
Author(s):  
Yuri Y. Kiryachkov ◽  
Marina V. Petrova ◽  
Bagautdin G. Muslimov ◽  
Sergey A. Bosenko ◽  
Mikhail M. Gorlachev
Keyword(s):  
Intervention Group ◽  
Clinical Effectiveness ◽  
Protective Role ◽  
Digital Data ◽  
Controlled Study ◽  
Control Group ◽  
Average Dose ◽  
Autonomic Nervous System Dysfunction ◽  
Sympathetic Hyperactivity ◽  
Starting Dose

Background.At the same time, the main effect of the use of this drug is the elimination of the autonomic nervous system dysfunction and sympatholysis. It seems important to search for a method of indications and selection of a dose of dexmedetomidine in intensive care.Aims to improve the clinical effectiveness of the electrophysiological navigation of the prolonged use of dexmedetomidine in patients with brain pathology of various origins.Methods.The study included 83 patients 2050 days after the traumatic brain injury, anoxic damage; consequences of acute disorders of cerebral. 37 patients comprised the 1st intervention group with a clinical course of dexmedetomidine (male 28; female 9; average age 49.62.3 years) and 46 patients comprised the 2nd control group without pharmacological correction with dexmedetomidine (male 23; female 23, average age 512.5 years). Criteria for the inclusion of prolonged infusion of the drug dexmedetomidine (Orion Pharma, Finland) are based on heart rate variability (HRV) indicators characteristic of sympathetic hyperactivity, the target task of titration of doses of dexmedetomidine served as the parameters for achieving normal HRV indicators, the appearance of parasympathetic hyperactivity served as the basis for reducing the dosage of the drug or stopping it of application. HRV parameters were recorded before dexmetomedine infusion-initially, on 13; 45; 910; 1520 days of drug administration.Results.The starting dose of dexmedetomidine with sympathetic hyperactivity in patients was 0.12 to 0.24 g.kg1.hr1(average dose 0.160.01; total 200 mg/day). According to digital data from HRV, the effective dose of dexmedetomidine ED50 was 0.260.03 g.kg1.hr1(total daily 353.835.1 g) and was achieved on day 910 using dexmedetomidine.Conclusions.The protective role of dexmedetomidine with correction of sympathetic hyperactivity based on electrophysiological navigation according to the HRV is reliable in the following indicators: The improvement of consciousness; a significant decrease in the incidence of distress lung syndrome; septic shock; mortality.

scholarly journals BuPiHeWei Decoction Ameliorates 5-Fu-Induced Intestinal Mucosal Injury in the Rats by Regulating the TLR-4/NF-κB Signaling Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Zhi-gao Sun ◽  
Ya-zhuo Hu ◽  
Yu-guo Wang ◽  
Jian Feng ◽  
Yong-qi Dou
Keyword(s):  
Body Weight ◽  
Signaling Pathway ◽  
Bacillus Licheniformis ◽  
Mucosal Injury ◽  
Protective Role ◽  
Inflammatory Factors ◽  
Control Group ◽  
Sprague Dawley ◽  
Intestinal Mucosal Injury ◽  
Group 5

BuPiHeWei (BPHW) decoction, a classic Traditional Chinese Medicinal (TCM) prescription, has been widely used in clinical practice to relieve digestive symptoms caused by chemotherapy, such as diarrhea and vomiting. The present study aimed to investigate whether BPHW decoction exerted a protective role in the 5-Fu-induced intestinal mucosal injury in the rats by regulating the mechanisms of TLR-4/NF-κB signaling pathway. There were 35 Sprague Dawley rats randomly divided into four groups: normal control group, 5-Fu group, 5-Fu + BPHW decoction group (10.5 g/kg, for five continuous days), and 5-Fu + Bacillus licheniformis capsule group (0.2 g/kg, for five continuous days). Animal models were established by intraperitoneal injection of 5-Fu (30 mg/Kg, for five consecutive days). At the end of the treatment period, body weight, diarrhea score, and histological examination were examined. Furthermore, the expression of TLR-4/NF-κB pathway was detected to reveal its mechanism. The results showed that BPHW decoction effectively reduced diarrhea score and increased body weight and height of villi after 5-Fu chemotherapy. In addition, BPHW decoction could significantly inhibit the expression of TLR-4, NF-κB, and inflammatory factors (including TNF-α, IL-1β, and IL-6) in the intestine, and the efficacy was significantly higher than that of Bacillus licheniformis capsule. In summary, BPHW decoction might be considered an effective drug to alleviate intestinal mucosal injury in the rats induced by 5-Fu.

scholarly journals Mechanism of SQQX Decoction’s Protective Effect on SHR: A Serum Metabolomics-Based Analysis

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Jiayun Wu ◽  
Lingling Li ◽  
Lin Li ◽  
Yanjun Li ◽  
Xiang Xiao ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Protective Effect ◽  
Metabolic Profiling ◽  
Transforming Growth Factor ◽  
Cholesterol Metabolism ◽  
Pharmaceutical Preparation ◽  
Intervention Group ◽  
Partial Least Square ◽  
Control Group ◽  
Optimal Dosage

SangQiQingXuan (SQQX) decoction is a pharmaceutical preparation exerting good therapeutic efficacy on high blood pressure (BP) and has widely been accepted in primarily hypertensive patients as a herbal formula prescribed by Professor Li Huang from China-Japan Friendship Hospital according to her 30-year clinical experience. A previous study showed that SQQX could reduce BP by decreasing levels of many inflammatory factors such as transforming growth factor beta (TGFβ) and elevating peroxisome proliferator activated receptor (PPAR) expression. However, a research focusing on SQQX’s protection against HTN from a metabolomic perspective has never been done before. This study aimed to figure out the metabolic profiling variations due to oral administration of SQQX in spontaneous hypertensive rat (SHR) models and to find out the optimal dosage of SQQX. SHR in the intervention group orally received SQQX extract of three doses, namely, the low- (5.25 g/kg/d), middle- (10.5 g/kg/d), and high-dosage groups (21 g/kg/d) for 90 days. Rats were sacrificed at the end of the experiment, and their serum was collected for further examination. Serum metabolic profiling variations were analyzed using ultraperformance liquid chromatography coupled with tandem mass spectrometry (UPLC/MS). Results showed that dealing with SQQX remarkably decreased systolic blood pressure (SBP) of SHRs and the high-dosage group was with the best therapeutic effect where a total of 11 metabolites were markedly changed in contrast to the model group. Orthogonal partial least square discriminant analysis (OPLS-DA) score plot showed that the 5 groups of serum samples were divided into 5 categories, and the metabolic trajectory of the high-dosage SQQX group was inclined to move to the control group. Glycochenodeoxycholic acid, nicotinamide-N-oxide, and tryptophan betaine might be biomarkers that specifically marked the protective effects of SQQX against high BP mainly involving in cholesterol metabolism, primary bile acid biosynthesis, bile secretion, and nicotinate and nicotinamide metabolism. To conclude, SQQX has a protective effect on SHR, which may be partially correlated to restoration of perturbed metabolism in serum.

scholarly journals Study on the Protective Effect of Special Electromagnetic Field Treated Water and Far Infrared Rays on LPS-Induced ARDS Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Ruiyin Huang ◽  
Yan Li ◽  
Xin Li ◽  
Qian Wang ◽  
Changyong Luo ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Protective Effect ◽  
Lung Tissue ◽  
Far Infrared ◽  
Inflammatory Factors ◽  
Control Group ◽  
Model Group ◽  
Blank Control Group ◽  
Treated Water ◽  
Infrared Rays

Objective. To explore the protective effect of special electromagnetic field treated water (SEW) and far infrared rays (IFR) on endotoxin (lipopolysaccharide, LPS) induced ARDS rats and the effect on inflammatory factors. Methods. 40 healthy male SD rats were randomly divided into 4 groups with 10 in each group. Preventive experiment: Adaptive feeding was carried out for 1 week according to animal feeding standards. Rats in SEW group drank SEW daily. Samely, rats in SEW and FIR group also drank SEW daily. Besides, rats in SEW and FIR group were also given far infrared rays for 20min/d. Rats in model group drank distilled water daily. After 7 days, rats in each group were injected with LPS (2 mg/kg) via the tail vein for making models. Rats in blank control group were given distilled water for 7 days, without modeling. All rats in the 4 groups were put to death under anesthesia 16 hours after modeling. Lung tissue and abdominal aortic blood were taken from these rats. Results. Pathological observation of lung and lung tissue indicated that rats in model group showed great pathological difference from rats in blank group. Rats in intervention group showed more symptomatic improvement in relation to alveolar and pulmonary interstitial congestion, edema, and inflammatory cell infiltration than rats in model group. The level of inflammatory factors like IL-1β and IL-6 in serum of rats in model group increased compared to blank control group (p<0.05). Comparing SEW group and SEW and FIR group with model group, levels of IL-1β and IL-6 in serum of rats both decreased remarkably (IL-Iβ: P < 0.05; IL-6: P < 0.01) while there was no obvious difference between SEW group and SEW and FIR group (p>0.05). The lung coefficient (LI) in SEW and FIR group was significantly lower than that in model group (P<0.01), which was higher than that of blank control group (P < 0.05), while there was no obvious difference between model group and SEW group (P>0.05). Compared with blank control group, lung permeability index (LPI) in model group showed no obvious difference (P>0.05). Conclusion. Special electromagnetic field treated water and far infrared rays can alleviate lung tissue damage of endotoxin-induced ARDS rats, relieving symptoms of alveolar and pulmonary interstitial congestion, edema, and inflammatory cell infiltration. The protective effect of special electromagnetic field treated water and far infrared rays on endotoxin-induced acute respiratory distress syndrome may result from their role in reducing the levels of IL-1β and IL-6 in serum and the expression level of p65 protein in lung tissue, in addition to reliving inflammatory response, lung coefficient, and lungs edema.

scholarly journals Mechanism of QingHuaZhiXie Prescription Regulating TLR4-IECs Pathway in the Intervention of Diarrhea Predominant Irritable Bowel Syndrome

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Hua Huang ◽  
Ping Zhao ◽  
Meijuan Xi ◽  
Fang Li ◽  
Lijiang Ji
Keyword(s):  
Irritable Bowel Syndrome ◽  
Intervention Group ◽  
Immunohistochemical Analysis ◽  
Inflammatory Factors ◽  
Control Group ◽  
Model Group ◽  
Expression Levels ◽  
Protein Levels ◽  
Intestinal Hypersensitivity ◽  
Irritable Bowel

To investigate the effect and mechanism of QingHuaZhiXie prescription on diarrhea predominant irritable bowel syndrome (D-IBS), animal models of rats were used in this study. 48 rats were randomly divided into 6 groups, containing one control group, one animal model group (D-IBS group), and four drug intervention groups (low, medium, and high dosage of QingHuaZhiXie prescription and trimebutine maleate intervention group). Abdominal withdrawal reflex (AWR) and Bristol stool form scale were recorded; the expression levels of inflammatory factors (TNF-α and IFN-γ), pathway proteins TLR4, MyD88, NF-κB, and key proteins of tight junction between intestinal epithelial cells (IECs) were detected; the microstructure of intestinal mucosal was observed by hematoxylin and eosin (H&E) staining; MPO activity was detected with immunohistochemical analysis to reflect the inflammation of tissues. Results show that QingHuaZhiXie prescription reduced diarrhea index and intestinal hypersensitivity and intestinal tissue integrity after intervention. MPO activity in QingHuaZhiXie prescription-treated rats was significantly lower relative to their model group. The expression levels of inflammatory factors and TLR4/MyD88/NF-κB pathway proteins were repressed, and the protein levels of occludin and claudin-1 increased. Meanwhile, this study also found that the remission effect of QingHuaZhiXie prescription on D-IBS increased with its dosage increase. Hence, as a therapeutic prescription for D-IBS, QingHuaZhiXie prescription could relieve D-IBS symptoms through balancing the inflammatory factors expression by inhibiting the TLR4/MyD88/NF-κB pathway and maintaining the function and structure of IECs by improving the protein levels of JAM, occludin, claudin-1, and ZO-1.

scholarly journals Astaxanthin Alleviates Ochratoxin A-Induced Cecum Injury and Inflammation in Mice by Regulating the Diversity of Cecal Microbiota and TLR4/MyD88/NF-κB Signaling Pathway

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Yueli Chen ◽  
Shiwei Zhao ◽  
Danyang Jiao ◽  
Beibei Yao ◽  
Shuhua Yang ◽  
...  
Keyword(s):  
Body Weight ◽  
Signaling Pathway ◽  
Ochratoxin A ◽  
Biological Activities ◽  
Intervention Group ◽  
Short Chain Fatty Acids ◽  
Environmental Pollutant ◽  
Inflammatory Factors ◽  
Control Group ◽  
Mucin 1

Ochratoxin A (OTA) is a common environmental pollutant found in a variety of foods and grains, and excessive OTA consumption causes serious global health effects on animals and humans. Astaxanthin (AST) is a natural carotenoid that has anti-inflammatory, antiapoptotic, immunomodulatory, antitumor, antidiabetes, and other biological activities. The present study is aimed at investigating the effects of AST on OTA-induced cecum injury and its mechanism of action. Eighty C57 mice were randomly divided into four groups, including the control group, OTA group (5 mg/kg body weight), AST group (100 mg/kg body weight), and AST intervention group (100 mg/kg body weight AST+5 mg/kg body weight OTA). It was found that AST decreased the endotoxin content, effectively prevented the shortening of mouse cecum villi, and increased the expression levels of tight junction (TJ) proteins, consisting of occludin, claudin-1, and zonula occludens-1 (ZO-1). AST increased the number of goblet cells, the contents of mucin-2 (MUC2), and defensins (Defa5 and β-pD2) significantly, while the expression of mucin-1 (MUC1) decreased significantly. The 16S rRNA sequencing showed that AST affected the richness and diversity of cecum flora, decreased the proportion of lactobacillus, and also decreased the contents of short-chain fatty acids (SCFAs) (acetate and butyrate). In addition, AST significantly decreased the expression of TLR4, MyD88, and p-p65, while increasing the expression of p65. Meanwhile, the expression of inflammatory factors including TNF-α and INF-γ decreased, while the expression of IL-10 increased. In conclusion, AST reduced OTA-induced cecum injury by regulating the cecum barrier function and TLR4/MyD88/NF-κB signaling pathway.

scholarly journals PROTECTIVE EFFECT OF ZINC;

2017 ◽  
Vol 24 (04) ◽  
pp. 580-588
Author(s):  
Kaukab Anjum ◽  
Asma Ali ◽  
Uzma Shahid
Keyword(s):  
Protective Effect ◽  
Protective Role ◽  
Diet Group ◽  
High Salt ◽  
Control Group ◽  
Analytical Control ◽  
High Salt Diet ◽  
Salt Diet ◽  
Medical College ◽  
Beneficial Effects

Background: Nutrition is an important determinant of bone health. Micronutrients,other than calcium has been paid less attention to-date in the prevention and treatment of bonediseases. Objective: To evaluate the protective effect of zinc on high salt induced gross changesin humerus and femur of rats. Study design: Analytical control randomized trial. Place andduration of study: Islamic International Medical College, Rawalpindi, hosted the research withthe cooperation of National Institute of Health; Islamabad. The study was approved by Ethicalreview committee of Riphah international university before its initiation. It took six months tocomplete the research (Sep 2015-March 2016). Material and methods: Forty five adult femaleSprague Dawley, 10-12 weeks old rats were used in the study. Three groups were made, eachhaving fifteen rats. Control group C (N=15) received laboratory diet without any alteration.Experimental group A (N=15) were served with high salt diet (8%NaCl) whereas experimentalgroup B (N=15) animals were given high salt diet augmented with zinc (50mg/kg/day).Allgroups were given the diet for eight weeks. Animals were weighed at the start and end ofstudy after which they were sacrificed. Left humeri and femora of all rats were obtained. Weightand mid shaft diameters of bones were recorded. The results were compiled after comparisonamongst all the groups. Results: Marked gross changes were witnessed in experimentalgroups. These changes were of greater severity in high salt diet group as compared to the zincsupplemented group in which reverse beneficial effects were noticed. After zinc administration,there was substantial increase in the weight of animals and bones with concurrent increase inmid shaft diameters. Conclusion: Zinc has a Protective role against high salt induced damageon the gross parameters of bones.

Ganoderma lucidum polysaccharides protect against sepsis-induced cardiac dysfunction by activating SIRT1

2021 ◽  
Author(s):  
Zhong-Heng Xu ◽  
Xiao Su ◽  
Geng Yang ◽  
Tao Qin ◽  
Yu Liu
Keyword(s):  
Cardiac Dysfunction ◽  
Ganoderma Lucidum ◽  
Inflammatory Cells ◽  
Protective Role ◽  
Inflammatory Factors ◽  
Control Group ◽  
Tunel Staining ◽  
Inflammatory Factor ◽  
Protective Effects ◽  
Ganoderma Lucidum Polysaccharides

Abstract Objective To investigate whether the silent information regulator 1 (SIRT1) was involved in the protective effects of Ganoderma lucidum polysaccharides (GLP) against sepsis-induced cardiac dysfunction. Methods Lipopolysaccharide (LPS)-induced sepsis model was constructed in C57/BL6J mice. Mice were randomly divided into LPS + GLP + EX-527, LPS + EX-527, LPS + GLP, LPS or control group). The levels of serum inflammatory factor markers were examined by ELISA. H&E staining was performed to assess the inflammation. TUNEL staining and bromodeoxyuridine staining were used to observe cell apoptosis and proliferation, respectively. Expression of apoptosis and proliferation-related proteins was detected by western blot. Key findings GLP treatment could significantly increase the expression of SIRT1, reduce levels of serum inflammatory factors (TNF-α, IL-1α and IL-6) and inflammatory cells in mice heart tissue of sepsis models (all Ps &lt; 0.01). Compared with LPS group, GLP treatment inhibited apoptosis and promoted proliferation of myocardial tissues (all Ps &lt; 0.01). Besides, EX-527 (SIRT1 inhibitor) treatment could partially reverse the protective effects of GLP against sepsis-induced cardiac dysfunction (all Ps &lt; 0.01). Conclusions GLP might play a protective role in sepsis-induced cardiac dysfunction through regulating inflammatory response, apoptosis and proliferation via activating SIRT1. Therefore, GLP is expected to be a probable novel strategy for treatment of sepsis.

scholarly journals The prophylactic effect of bovine colostrum on respiratory infection and diarrhea in formula-fed infants: a randomized trial

2020 ◽  
Author(s):  
Ke Chen ◽  
Haixia Chen ◽  
Jinwen Luo ◽  
Chenggui Zeng ◽  
Xiaobing Dong ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Respiratory Infection ◽  
Respiratory Tract Infections ◽  
Intervention Group ◽  
Protective Role ◽  
Bovine Colostrum ◽  
Control Group ◽  
Prophylactic Effect ◽  
Term Infants ◽  
Tract Infections

Abstract Background It has been reported that regular consumption of bovine colostrum has a protective role for the gastrointestinal immunity of infants. However, not enough investigation has explored the prophylactic effect of bovine colostrum on the morbidity of diarrhea and respiratory tract infections in formula-fed infants. The purpose of the study is to explore the prophylactic effect of BC on morbidity of diarrhea and respiratory tract infections in formula-fed infants.Methods A total of 192 term infants aged 6 to 9 months, who were previously fully formula-fed after birth, were randomly recruited into this multi-centric, randomized, blank-controlled, opened, and post-marketing intervention study. Infants in the intervention group (IG) were given a commercially available bovine colostrum sachet once a day for 3 months while infants in the control group (CG) were not given the bovine colostrum sachet. The morbidity and duration of diarrhea and respiratory tract infections and the fecal IgA, calprotectin, and total fatty acid were monitored during the intervention.Result There was a lower incidence of loose stool [IG/CG rate ratio: 0.22, 95%CI: 0.09-0.67], increased stool frequency (0.30, 0.17-0.78), loss of appetite (0.28, 0.11-0.47), sneezing (0.22,0.07-0.69), upper respiratory infection (0.55, 0.32-0.88), and diarrhea (0.25, 0.09-0.61) and shorter duration of diarrhea for infants in IG than those in CG.Conclusion Bovine colostrum intervention for 3 months showed a potential prophylactic effect on respiratory infection and diarrhea in formula-fed infants. The present data may be applicable to other infants of similar socioeconomic status.

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