scholarly journals Calibration of analytical HPLC to generate preparative LC gradients for peptide purification

2018 ◽  
Author(s):  
Jack Silver
Keyword(s):  
Peptide Purification ◽  
Preparative Lc ◽  
Analytical Hplc

Simultaneous Separation of Four Flavonoids and Two Astragalosides from Radix Astragali by Semi-Preparative LC

2010 ◽  
Vol 71 (3-4) ◽  
pp. 225-231 ◽  
Author(s):  
Xinrong Dong ◽  
Yizeng Liang ◽  
Bing Wang ◽  
Xiaohong Long
Keyword(s):  
Radix Astragali ◽  
Simultaneous Separation ◽  
Preparative Lc

scholarly journals Traceless parallel peptide purification by a first-in-class reductively cleavable linker system featuring a safety-release

2021 ◽  
Vol 12 (7) ◽  
pp. 2389-2396
Author(s):  
Robert Zitterbart ◽  
Nadja Berger ◽  
Oliver Reimann ◽  
Gavin T. Noble ◽  
Stephan Lüdtke ◽  
...  
Keyword(s):  
Peptide Purification ◽  
Rapid Production

A first-in-class reductively cleavable linker system that enables parallel and traceless purification of peptides through a safety-release is introduced with three linker types and showcased by rapid production of 20 personalized neoantigen peptides.

Fmoc as a Probe in Peptide Purification

2009 ◽  
pp. 197-200
Author(s):  
Jiangli Song ◽  
Zhenchao Gao ◽  
Lanlan You ◽  
Niansheng Li ◽  
XianGang Yang ◽  
...  
Keyword(s):  
Peptide Purification

Determination of Melengestrol Acetate in Feedstuffs with Liquid Chromatographic Preparatory Column Cleanup and Quantitative Analysis

1988 ◽  
Vol 71 (4) ◽  
pp. 707-709
Author(s):  
Jim L Weigand ◽  
Daniel S Dille
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Sodium Sulfate ◽  
Alumina Column ◽  
Melengestrol Acetate ◽  
Preparative Lc ◽  
Time Savings ◽  
Commercial Feeds ◽  
Liquid Chromatographic

Abstract Melengestrol acetate (MGA) is determined by liquid chromatography using a fraction from preparatory LC as a means of sample cleanup for feedstuffs, both dry and liquid. Dry ground feed is Soxhlet extracted with hexane and passed through a 2% deactivated alumina column for initial cleanup. The eluate is evaporated, redissolved in methanol, filtered, and injected onto a preparatory LC column. The fraction containing MGA is separated from the remaining matrix, evaporated to dryness, dissolved in methanol, and quantitated by LC analysis. Liquid supplements are extracted in methanol, and the extract is evaporated to near dryness. The residue is diluted with water, extracted with chloroform, passed through sodium sulfate, and evaporated to dryness. The remaining sample is dissolved in methanol prior to preparative LC and quantitative LC. Recoveries for 2 laboratory- fortified commercial feeds, one dry and one liquid, containing 0.39 and 0.40 mg/lb, were 98.3% ± 4.4 and 95.8% ± 4.3, respectively. Results compare favorably with existing methods. Up to a 4-fold time savings was realized by this method without automation.

A Simple Design to Realize Micro-column Separation by Conventional Analytical HPLC

2009 ◽  
Vol 27 (4) ◽  
pp. 763-767 ◽  
Author(s):  
Wenjun GONG ◽  
Junxia ZHANG ◽  
Yuping ZHANG ◽  
Yijun ZHANG ◽  
Mengkui TIAN ◽  
...  
Keyword(s):  
Simple Design ◽  
Column Separation ◽  
Analytical Hplc
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