scholarly journals Antibiotic resistance among Escherichia coli urinary isolates and their susceptibility to clove essential oil

2018 ◽  
Vol 71 (2) ◽  
pp. 41
Author(s):  
Michał Dąbrowski ◽  
Monika Sienkiewicz ◽  
Hanna Zielińska-Bliźniewska ◽  
Marta Dąbrowska ◽  
Małgorzata Seredyńska ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Essential Oil ◽  
Urinary Tract Infections ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Diffusion Method ◽  
Syzygium Aromaticum ◽  
Tract Infections

<p>Escherichia coli is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium, which is a primary cause of urinary tract infections. Resistance to antibiotics has become a particular problem in recent decades. Consequently, there is an unmet need for new therapeutic options. It has been observed that essential oils have bactericidal effects. The antimicrobial susceptibility testing for Escherichia coli isolates obtained from urine of patients with urinary tract infections was determined via disk diffusion method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST, 2015). Essential oil from clove – Syzygium aromaticum (L.) Merill et L.M. (Myrtaceae) was analyzed by GC-FID-MS. Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) were detected by using the micro-dilution broth method. Escherichia coli clinical isolates are characterized by high resistance to ampicillin, amoxicillin with clavulanic acid, norfloxacin, trimethoprim/sulfamethoxazole, tetracycline, tobramycin and ticarcillin. Clove oil possesses strong inhibiting and killing properties against E. coli isolates, among them the ones resistant to recommended antibiotics. The results of this study highlight the need for testing the efficacy of new agents to inactivate bacteria in clinical settings.</p>

An automated and portable antimicrobial susceptibility testing system for urinary tract infections

Lab on a Chip ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 755-763
Author(s):  
Kuo-Wei Hsu ◽  
Wen-Bin Lee ◽  
Huey-Ling You ◽  
Mel S. Lee ◽  
Gwo-Bin Lee
Keyword(s):  
Urinary Tract ◽  
Minimum Inhibitory Concentration ◽  
Urinary Tract Infections ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Inhibitory Concentration ◽  
Microfluidic System ◽  
Antimicrobial Susceptibility Testing ◽  
Testing System ◽  
Tract Infections

A portable, integrated microfluidic system capable of automatically conducting antimicrobial susceptibility testing (AST) and minimum inhibitory concentration (MIC) measurements using urine samples were developed.

Change in antimicrobial susceptibility of Escherichia coli urinary tract isolates at a single institution over a period of 10 years

2012 ◽  
Vol 58 (3) ◽  
pp. 345-349 ◽  
Author(s):  
A. Walkty ◽  
P.R.S. Lagacé-Wiens ◽  
J.A. Karlowsky ◽  
D.J. Hoban ◽  
K. Manickam ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Single Institution ◽  
Entire Study ◽  
E Coli ◽  
Catheter Urine ◽  
Tract Infections

Urinary tract infections are common. Few published studies have demonstrated the change in Escherichia coli urinary isolate antimicrobial susceptibility over time within a given area and (or) population. The purpose of this study was to evaluate the change in susceptibility of E. coli clinical isolates obtained from urine specimens at a single institution over a period of 10 years. The microbiology laboratory information system at St. Boniface Hospital (Winnipeg, Manitoba, Canada) was searched retrospectively from 1 January 2000 to 31 December 2009, for all E. coli isolates from either a midstream or catheter urine source that had susceptibility testing performed. Only one isolate per patient was included during the entire study period. Antimicrobial susceptibility testing was carried out with either a Microscan instrument (pre-April 2004) or a Vitek instrument (May 2004 onwards). In total, 7353 E. coli urinary isolates were included for evaluation. Ciprofloxacin susceptibility declined significantly, from 99% in 2000 to 85% in 2009 (p < 0.0001). A small but statistically significant decline in susceptibility was also observed for ampicillin, cefazolin, trimethoprim–sulfamethoxazole, gentamicin, and nitrofurantoin. These data suggest that certain antimicrobials recommended for the treatment of urinary tract infections (ciprofloxacin, trimethoprim–sulfamethoxazole) may no longer be optimal.

scholarly journals Diagnosis of Urinary Tract Infections in Children

2016 ◽  
Vol 54 (9) ◽  
pp. 2233-2242 ◽  
Author(s):  
Christopher D. Doern ◽  
Susan E. Richardson
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Laboratory Diagnosis ◽  
Antimicrobial Susceptibility Testing ◽  
Special Consideration ◽  
Common Occurrence ◽  
Specimen Collection ◽  
Tract Infections

Urinary tract infections (UTIs) are a common occurrence in children. The management and laboratory diagnosis of these infections pose unique challenges that are not encountered in adults. Important factors, such as specimen collection, urinalysis interpretation, culture thresholds, and antimicrobial susceptibility testing, require special consideration in children and will be discussed in detail in the following review.

scholarly journals First Detection of CTX-M and SHV Extended-Spectrum β-Lactamases in Escherichia coli Urinary Tract Isolates from Dogs and Cats in the United States

2010 ◽  
Vol 54 (8) ◽  
pp. 3489-3492 ◽  
Author(s):  
Alexandra O'Keefe ◽  
Tabitha A. Hutton ◽  
Dieter M. Schifferli ◽  
Shelley C. Rankin
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
The United States ◽  
Extended Spectrum ◽  
Tract Infections ◽  
M 14

ABSTRACT One hundred fifty canine and feline Escherichia coli isolates associated with urinary tract infections were screened for the presence of extended-spectrum β-lactamase (ESBL) genes. Out of 60 isolates suspected to be ESBL positive based on antimicrobial susceptibility testing, 11 ESBLs were identified, including one SHV-12 gene, one CTX-M-14 gene, and nine CTX-M-15 genes. This study provides the first report of CTX-M- and SHV-type ESBLs in dogs and cats in the United States.

scholarly journals Antimicrobial Susceptibility of Community Acquired Escherichia coli in Urinary Tract Infections (UTI) in Benin for Eleven Years (2005-2015)

2017 ◽  
Vol 13 (2) ◽  
pp. 21-27
Author(s):  
Tchiakpe Edmond ◽  
Laurence Carine Yehouenou ◽  
Zahra Fall Malick ◽  
Kpangon Amadohou&eacute; Ars&egrave;ne ◽  
Keke Kpemahouton Ren&eacute; ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antimicrobial Susceptibility ◽  
Tract Infections
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