Phosphorescence lifetime imaging in turbid media

2021 ◽  
Author(s):  
Vadim Soloviev
Keyword(s):  
Turbid Media ◽  
Phosphorescence Lifetime ◽  
Lifetime Imaging ◽  
Phosphorescence Lifetime Imaging

Phosphorescence lifetime imaging in turbid media: the forward problem

2003 ◽  
Vol 42 (1) ◽  
pp. 113 ◽  
Author(s):  
Vadim Soloviev ◽  
David Wilson ◽  
Sergei Vinogradov
Keyword(s):  
Forward Problem ◽  
Turbid Media ◽  
Phosphorescence Lifetime ◽  
Lifetime Imaging ◽  
Phosphorescence Lifetime Imaging

scholarly journals Biocompatible Ir(III) Complexes as Oxygen Sensors for Phosphorescence Lifetime Imaging

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2898
Author(s):  
Ilya S. Kritchenkov ◽  
Anastasia I. Solomatina ◽  
Daria O. Kozina ◽  
Vitaly V. Porsev ◽  
Victor V. Sokolov ◽  
...  
Keyword(s):  
Near Infrared ◽  
Biological Systems ◽  
Aqueous Media ◽  
Oxygen Sensors ◽  
Quantum Yields ◽  
Complete Agreement ◽  
Phosphorescence Lifetime ◽  
Lifetime Imaging ◽  
Nir Emission ◽  
Phosphorescence Lifetime Imaging

Synthesis of biocompatible near infrared phosphorescent complexes and their application in bioimaging as triplet oxygen sensors in live systems are still challenging areas of organometallic chemistry. We have designed and synthetized four novel iridium [Ir(N^C)2(N^N)]+ complexes (N^C–benzothienyl-phenanthridine based cyclometalated ligand; N^N–pyridin-phenanthroimidazol diimine chelate), decorated with oligo(ethylene glycol) groups to impart these emitters’ solubility in aqueous media, biocompatibility, and to shield them from interaction with bio-environment. These substances were fully characterized using NMR spectroscopy and ESI mass-spectrometry. The complexes exhibited excitation close to the biological “window of transparency”, NIR emission at 730 nm, and quantum yields up to 12% in water. The compounds with higher degree of the chromophore shielding possess low toxicity, bleaching stability, absence of sensitivity to variations of pH, serum, and complex concentrations. The properties of these probes as oxygen sensors for biological systems have been studied by using phosphorescence lifetime imaging experiments in different cell cultures. The results showed essential lifetime response onto variations in oxygen concentration (2.0–2.3 μs under normoxia and 2.8–3.0 μs under hypoxia conditions) in complete agreement with the calibration curves obtained “in cuvette”. The data obtained indicate that these emitters can be used as semi-quantitative oxygen sensors in biological systems.

scholarly journals Water-soluble cyclometalated platinum(ii) and iridium(iii) complexes: synthesis, tuning of the photophysical properties, and in vitro and in vivo phosphorescence lifetime imaging

RSC Advances ◽  
2018 ◽  
Vol 8 (31) ◽  
pp. 17224-17236 ◽  
Author(s):  
Anastasia I. Solomatina ◽  
Shih-Hao Su ◽  
Maria M. Lukina ◽  
Varvara V. Dudenkova ◽  
Vladislav I. Shcheslavskiy ◽  
...  
Keyword(s):  
Photophysical Properties ◽  
Water Soluble ◽  
Iridium Complexes ◽  
Hypoxia Imaging ◽  
Phosphorescence Lifetime ◽  
Lifetime Imaging ◽  
Phosphorescence Lifetime Imaging

Novel water-soluble iridium complexes with sulfonated diphosphine allow in vitro and in vivo lifetime hypoxia imaging.

Phosphorescence Lifetime Imaging of Labile Zn2+ in Mitochondria via a Phosphorescent Iridium(III) Complex

2018 ◽  
Vol 57 (17) ◽  
pp. 10625-10632 ◽  
Author(s):  
Changli Zhang ◽  
Minsheng Liu ◽  
Shaoxian Liu ◽  
Hui Yang ◽  
Qiang Zhao ◽  
...  
Keyword(s):  
Phosphorescence Lifetime ◽  
Lifetime Imaging ◽  
Phosphorescence Lifetime Imaging

scholarly journals Monitoring mitochondrial viscosity with anticancer phosphorescent Ir(iii) complexes via two-photon lifetime imaging

2019 ◽  
Vol 10 (5) ◽  
pp. 1285-1293 ◽  
Author(s):  
Liang Hao ◽  
Zhi-Wei Li ◽  
Dong-Yang Zhang ◽  
Liang He ◽  
Wenting Liu ◽  
...  
Keyword(s):  
Mitochondrial Dysfunction ◽  
Phosphorescence Lifetime ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Phosphorescence Lifetime Imaging

A theranostic Ir(iii) complex is developed to induce mitochondrial dysfunction and monitor mitochondrial viscosity quantitatively via two-photon phosphorescence lifetime imaging.

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