Novel Biomimetic Aggrecan for the Acellular Regeneration of the Intervertebral Disc: Synthesis, Enzymatic Stability and Molecular Engineering

2021 ◽  
Author(s):  
Sarah Eugenia Lightfoot Vidal
Keyword(s):  
Intervertebral Disc ◽  
Molecular Engineering ◽  
Enzymatic Stability

Fluorescence ratio imaging of dynamic intracellular ionic signals

1988 ◽  
Vol 46 ◽  
pp. 42-43
Author(s):  
R. Y. Tsien ◽  
A. Minta ◽  
M. Poenie ◽  
J.P.Y. Kao ◽  
A. Harootunian
Keyword(s):  
Binding Sites ◽  
Living Cells ◽  
Molecular Engineering ◽  
Ph Indicators ◽  
Highly Sensitive ◽  
Fluorescence Ratio Imaging ◽  
Ratio Imaging ◽  
Technical Advances ◽  
Indicator Dyes ◽  
Fluorescein Dyes

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.

CCN2 Functions as Anti-Inflammatory Molecule in the Intervertebral Disc

2014 ◽  
Vol 4 (1_suppl) ◽  
pp. s-0034-1376756-s-0034-1376756
Author(s):  
Makarand V. Risbud
Keyword(s):  
Intervertebral Disc ◽  
Inflammatory Molecule ◽  
Anti Inflammatory

Loading-Induced Stress Response in the Intervertebral Disc

2014 ◽  
Vol 4 (1_suppl) ◽  
pp. s-0034-1376604-s-0034-1376604
Author(s):  
W. H. Chooi ◽  
S. C. Chan ◽  
B. Gantenbein-Ritter ◽  
B. P. Chan
Keyword(s):  
Stress Response ◽  
Intervertebral Disc ◽  
Induced Stress

Immortalization of Human Cartilage Endplate Stem Cell from Degenerated Human Intervertebral Disc

2014 ◽  
Vol 4 (1_suppl) ◽  
pp. s-0034-1376589-s-0034-1376589
Author(s):  
M. Liu ◽  
B. Huang ◽  
Y. Zhou
Keyword(s):  
Stem Cell ◽  
Intervertebral Disc ◽  
Human Cartilage ◽  
Cartilage Endplate

DNA Demethylation Regulates Anabolic and Catabolic Gene Expression in Intervertebral Disc Cells

2014 ◽  
Vol 4 (1_suppl) ◽  
pp. s-0034-1376587-s-0034-1376587
Author(s):  
N. Chutkan ◽  
R. Sangani ◽  
H. Zhou ◽  
S. Fulzele
Keyword(s):  
Gene Expression ◽  
Intervertebral Disc ◽  
Dna Demethylation ◽  
Catabolic Gene ◽  
Disc Cells

Melatonin: Protection of the Intervertebral Disc

2019 ◽  
Vol 2 (3) ◽  
pp. 1-9
Author(s):  
Russel J Reiter ◽  
Sergio Rosales-Corral ◽  
Ramaswamy Sharma
Keyword(s):  
Intervertebral Disc ◽  
Molecular Mechanisms ◽  
Intervertebral Discs ◽  
Lumbar Pain ◽  
Low Back ◽  
Work Time ◽  
Protective Actions ◽  
Aging Populations ◽  
Definition Of ◽  
Endogenous Melatonin

     Low back pain (lumbar pain) due to injury of or damage to intervertebral discs is common in all societies.  The loss of work time as a result of this problem is massive.  Recent research suggests that melatonin may prevent or counteract intervertebral disc damage. This may be especially relevant in aging populations given that endogenous melatonin, in most individuals, dwindles with increasing age. The publications related to melatonin and its protection of the intervertebral disc are reviewed herein, including definition of some molecular mechanisms that account for melatonin’s protective actions. 

Postoperative Changes of Herniated Intervertebral Disc: Normal and Discitis MR Findings

1994 ◽  
Vol 31 (2) ◽  
pp. 223
Author(s):  
Seung Jae Lim ◽  
Yup Yoon ◽  
Ki Tack Kim ◽  
Kyung Nam Ryu ◽  
Woo Suk Choi
Keyword(s):  
Intervertebral Disc ◽  
Postoperative Changes ◽  
Herniated Intervertebral Disc

Combinatorial Peptide Libraries for Selecting Inorganic-Binding Proteins: A Step in Molecular Biomimetics

2003 ◽  
Vol 773 ◽  
Author(s):  
C. Tamerler ◽  
S. Dinçer ◽  
D. Heidel ◽  
N. Karagûler ◽  
M. Sarikaya
Keyword(s):  
Binding Proteins ◽  
Building Blocks ◽  
Molecular Engineering ◽  
Inorganic Materials ◽  
Combinatorial Peptide Libraries ◽  
Self Assembling ◽  
Complex Functions ◽  
Recent Developments ◽  
Specific Proteins ◽  
Molecular Biomimetics

AbstractProteins, one of the building blocks in organisms, not only control the assembly in biological systems but also provide most of their complex functions. It may be possible to assemble materials for practical technological applications utilizing the unique advantages provided by proteins. Here we discuss molecular biomimetic pathways in the quest for imitating biology at the molecular scale via protein engineering. We use combinatorial biology protocols to select short polypeptides that have affinity to inorganic materials and use them in assembling novel hybrid materials. We give an overview of some of the recent developments of molecular engineering towards this goal. Inorganic surface specific proteins were identified by using cell surface and phage display technologies. Examples of metal and metal oxide specific polypeptides were represented with an emphasis on certain level of specificities. The recognition and self assembling characteristics of these inorganic-binding proteins would be employed in develeopment of hybrid multifunctional materials for novel bio- and nano-technological applications.

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