Analysis of Airborne Methicillin-Resistant Staphylococcus aureus and Gram Positive Coagulase Negative Staphylococcus species in Homes

2021 ◽  
Author(s):  
Karen Michael
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Coagulase Negative Staphylococcus ◽  
Gram Positive ◽  
Methicillin Resistant

scholarly journals Aktivitas Antimikroba Fraksi Etil Asetat Daun Ceremai [Phyllanthus acidus (L.) Skeels] terhadap Bakteri Resisten Antimikroba dan Jamur dengan Metode KLT Bioautografi

2020 ◽  
Vol 4 ◽  
Author(s):  
Lanny Mulqie ◽  
Kusnandar Anggadireja
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Coagulase Negative Staphylococcus ◽  
Microsporum Gypseum ◽  
Vancomycin Resistant Enterococcus ◽  
Methicillin Resistant ◽  
Phyllanthus Acidus ◽  
Vancomycin Resistant

Resistensi dan toksisitas antimikroba menyebabkan penggunaan obat herbal sebagai alternatif  pengobatan berbagai penyakit yang disebabkan oleh mikroba. Ekstrak etanol daun ceremai memiliki aktivitas terhadap VRE, MRCNS, dan Candida albicans. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba fraksi etil asetat daun ceremai terhadap bakteri resisten antimikroba [Methicillin-Resistant Staphylococcus aureus (MRSA), Methicillin-Resistant Coagulase Negative Staphylococcus (MRCNS), Vancomycin Resistant Enterococcus (VRE)] dan jamur uji (Candida albicans, Microsporum gypseum, dan Aspergillus niger) dengan metode KLT bioautografi. Fraksinasi dilakukan dengan cara ekstraksi cair-cair menggunakan pelarut dengan tingkat kepolaran yang berbeda, yaitu n-heksan, etil asetat, dan air. KLT bioautografi pada fraksi etil asetat daun ceremai dilakukan menggunakan fase diam silika gel GF254 dan pengembang etil asetat-asam format-asam asetat-air (100:10:10:22), menggunakan sitroborat sebagai penampak bercak. Pada hasil kromatogram terdapat 5 noda yang berfluoresensi hijau kekuningan dengan nilai rf noda 1 sebesar 0,35; noda 2 sebesar 0,43, noda 3 sebesar 0,62, noda 4 sebesar 0,75 dan noda 5 sebesar 0,90 setelah plat disemprot oleh sitroborat dan dilihat di bawah sinar UV. Hasil pengujian aktivitas antimikroba dengan metode KLT bioautografi menunjukkan terbentuknya zona bening yang dihasilkan noda pada plat yang ditempelkan pada media. Fraksi etil asetat daun ceremai memiliki aktivitas antimikroba terhadap bakteri resisten antimikroba (MRSA, MRCNS, dan VRE) dan jamur  uji (Candida albicans, Microsporum gypseum, dan Aspergillus niger) yang dihasilkan oleh noda 1 (rf 0,35), noda 2 (rf 0,43), noda 3 (rf 0,62), noda 4 (rf 0,75), dan noda 5 (rf 0,90). Golongan senyawa yang diduga memiliki aktivitas antimikroba adalah flavonoid.

scholarly journals Correlation between Vancomycin MIC Values and Those of Other Agents against Gram-Positive Bacteria among Patients with Bloodstream Infections Caused by Methicillin-Resistant Staphylococcus aureus

2009 ◽  
Vol 53 (12) ◽  
pp. 5141-5144 ◽  
Author(s):  
N. Patel ◽  
P. Lubanski ◽  
S. Ferro ◽  
M. Bonafede ◽  
S. Harrington ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Bloodstream Infections ◽  
Care Hospital ◽  
Gram Positive ◽  
Methicillin Resistant ◽  
Gram Positive Bacteria ◽  
Included Patient

ABSTRACT An increase in the distribution of vancomycin MIC values among methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) isolates has been noted. It is postulated that the shift in vancomycin MIC values may be associated with a concurrent rise in the MIC values of other anti-MRSA agents. Scant data are available on the correlation between vancomycin MIC values and the MIC values of other anti-MRSA agents. This study examined the correlation between vancomycin MIC values and the MIC values of daptomycin, linezolid, tigecycline, and teicoplanin among 120 patients with bloodstream infections caused by MRSA at a tertiary care hospital between January 2005 and May 2007. For each included patient, the MIC values of the antibiotics under study were determined by the Etest method and were separated into the following two categories: day 1 (index) and post-day 1 (subsequent). For subsequent isolates, the MIC values for each antibiotic from the post-day 1 terminal isolate were used. Among the index isolates, there was a significant correlation (P value, <0.01) between the MIC values for vancomycin and daptomycin and between the MIC values for vancomycin and teicoplanin. The MIC values for daptomycin were significantly correlated with linezolid, tigecycline, and teicoplanin MIC values. Among the 48 patients with subsequent isolates, vancomycin MIC values were significantly correlated with MIC values for daptomycin, linezolid, and teicoplanin (ρ value of ≥0.38 for all comparisons). This study documented an association between vancomycin MIC values and the MIC values of other anti-MRSA antibiotics among patients with bloodstream infections caused by MRSA primarily treated with vancomycin.

Sign in / Sign up

Export Citation Format

Share Document