Plasma and protein surface functionalization for three-dimensional polycaprolactone tissue scaffolds

2021 ◽  
Author(s):  
Eda Didem Yildirim
Keyword(s):  
Surface Functionalization ◽  
Three Dimensional ◽  
Tissue Scaffolds ◽  
Protein Surface

scholarly journals Complex three-dimensional graphene structures driven by surface functionalization

Nanoscale ◽  
2020 ◽  
Vol 12 (18) ◽  
pp. 10172-10179 ◽  
Author(s):  
Duc Tam Ho ◽  
Viet Hung Ho ◽  
Vasudeo Babar ◽  
Sung Youb Kim ◽  
Udo Schwingenschlögl
Keyword(s):  
Surface Functionalization ◽  
Three Dimensional ◽  
Form Complex ◽  
Graphene Structures

A self-folding approach inspired by the origami technique is developed to form complex three-dimensional graphene structures using pattern-based surface functionalization.

scholarly journals A Customizable, Low-Cost Perfusion System for Sustaining Tissue Constructs

2018 ◽  
Vol 23 (6) ◽  
pp. 592-598
Author(s):  
Brian J. O’Grady ◽  
Jason X. Wang ◽  
Shannon L. Faley ◽  
Daniel A. Balikov ◽  
Ethan S. Lippmann ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Large Scale ◽  
Low Cost ◽  
Three Dimensional ◽  
Tissue Scaffolds ◽  
Perfusion System ◽  
Pump System ◽  
Tissue Constructs ◽  
Engineered Tissue

The fabrication of engineered vascularized tissues and organs requiring sustained, controlled perfusion has been facilitated by the development of several pump systems. Currently, researchers in the field of tissue engineering require the use of pump systems that are in general large, expensive, and generically designed. Overall, these pumps often fail to meet the unique demands of perfusing clinically useful tissue constructs. Here, we describe a pumping platform that overcomes these limitations and enables scalable perfusion of large, three-dimensional hydrogels. We demonstrate the ability to perfuse multiple separate channels inside hydrogel slabs using a preprogrammed schedule that dictates pumping speed and time. The use of this pump system to perfuse channels in large-scale engineered tissue scaffolds sustained cell viability over several weeks.

Direct writing of porous tissue scaffolds based on Vaseline-doped hydroxyapatite inks

2015 ◽  
Vol 29 (14) ◽  
pp. 1550066
Author(s):  
Ya-Yun Li ◽  
Long-Tu Li ◽  
Bo Li
Keyword(s):  
Human Liver ◽  
Carcinoma Cell ◽  
Three Dimensional ◽  
Tissue Scaffolds ◽  
Liver Carcinoma ◽  
Test Results ◽  
Direct Writing ◽  
Precision Control ◽  
Direct Ink Writing ◽  
Carcinoma Cell Line Hepg2

A novel type of 40 vol.% hydroxyapatite ( HAp ), Ca 10( PO 4)6( OH )2, suspension doped with Vaseline was developed, and porous three-dimensional (3D) scaffolds were fabricated by using a direct ink writing (DIW) method. The preparation of the HAp inks and the principles of the DIW technique were investigated. The microporosity of the scaffold wall increased after introducing the Vaseline, whereas macroporosity can be produced by varying the DIW technique. The micromorphology test results show that the samples sintered at 1150°C for 2 h formed ceramics with a set amount of pores, which benefit cell growth by providing more locations for cells to attach and proliferate. Under a microscope, the proliferations of human liver carcinoma cell line ( HepG2 ) cells can be observed on the 3D HAp scaffolds. The DIW method has the advantages of a rapid process, ease of design and high precision control, potentially inspiring the design and application of biomaterials and scaffolds.

Fabrication of three-dimensional polycaprolactone/hydroxyapatite tissue scaffolds and osteoblast-scaffold interactions in vitro

Biomaterials ◽  
2007 ◽  
Vol 28 (35) ◽  
pp. 5291-5297 ◽  
Author(s):  
Lauren Shor ◽  
Selçuk Güçeri ◽  
Xuejun Wen ◽  
Milind Gandhi ◽  
Wei Sun
Keyword(s):  
Three Dimensional ◽  
Tissue Scaffolds

A Method for Fabrication of Polycarbonate-Based Bioactive Platforms

2008 ◽  
Vol 13 (5) ◽  
pp. 284-288 ◽  
Author(s):  
Peyman Najmabadi ◽  
Kwang-Seuk Ko ◽  
James J. La Clair ◽  
Michael D. Burkart
Keyword(s):  
Monoclonal Antibody ◽  
Surface Functionalization ◽  
Three Dimensional ◽  
Compression Molding ◽  
Chemical Derivatization ◽  
Proof Of Concept ◽  
Hot Press ◽  
Site Specific ◽  
Molecular Receptors ◽  
Three Dimensional Objects

Surface-based assays have been used extensively for the functional and structural analysis of biomolecules such as DNA or proteins. These experiments are established by the analysis of binding between acceptor molecules and immobilized receptors on a platform. Site-specific printing of receptor molecules on gold, glass, or polycarbonate (PC) surfaces is conventionally performed by the chemical derivatization of a surface, priming it to covalently bind to subsequently deposited receptor molecules. Unlike conventional methods, we have developed a new fabrication method for bioactive PC surfaces by directly molding PC granules doped with receptor molecules. PC-based receptor molecules were synthesized and commercially available PC granules were doped with these synthesized molecules. In our proof-of-concept study, PC doped with dye 1 ( Fig. 1 ) was used as the receptor molecule. Using an aluminum mold and a hot press machine, PC-based objects were manufactured through compression molding using doped PC granules. Affinity analysis was evaluated by monitoring the localization of a monoclonal antibody elicited against dye 1 to the surface of the molded platforms by fluorescence microscopy. The results illustrated effective binding of an anti-dye 1 monoclonal antibody to the surface, substantiating successful display of assemblies of molecular receptors on the surface through compression molding. Although conventional surface functionalization methods impose limited applications and alter desired opto-mechanical properties of the polymer, our investigation provides a versatile means for the fabrication of bioactive PC-based platforms. It can also be used for engineering and imbedding receptor arrays within three-dimensional objects with applications to the production of opto-medical devices or biosensors.

Surface functionalization of polyurethane for the immobilization of bioactive moieties on tissue scaffolds

2008 ◽  
Vol 18 (19) ◽  
pp. 2240 ◽  
Author(s):  
Andrzej B. Jóźwiak ◽  
Cay M. Kielty ◽  
Richard A. Black
Keyword(s):  
Surface Functionalization ◽  
Tissue Scaffolds

New Materials and Methods for Hierarchically Structured Tissue Scaffolds

2004 ◽  
Vol 845 ◽  
Author(s):  
Benita M. Comeau ◽  
Yusif Umar ◽  
Kenneth E. Gonsalves ◽  
Clifford L. Henderson
Keyword(s):  
Cell Growth ◽  
Control Cell ◽  
Three Dimensional ◽  
Physical Structure ◽  
Tissue Scaffolds ◽  
Structure Control ◽  
Polymeric Scaffold ◽  
Building Process ◽  
Multi Wavelength ◽  
Physical And Chemical

ABSTRACTThe overall goal of our work is to develop new methods and materials for the fabrication of hierarchically structured, three-dimensional (3D) tissue scaffolds. Conventional scaffolds commonly lack substantial mechanical strength, and there is difficulty in controlling porosity, pore distribution, and pore interconnectivity. Additionally, the chemical nature of these scaffolds is typically homogenous. The ability to chemically modify selected areas on a scaffold is one method to direct cell growth in deliberate patterns; which could aid in the engineering of complex, functioning tissues. The general aim of this work is to address these issues through the application of stereolithography (SL) to the fabrication of hierarchically structured scaffolds.In order to achieve this goal, photopolymerizable materials must be developed that are both compatible with cell growth and with SL processing. SL methods are designed to produce arbitrary control over the physical structure of the part. In addition to physical structure control, control over the local surface chemistry of the scaffold is also desired. This would permit the use of both physical and chemical cues to control cell behavior in a tissue engineering construct. Chemical control could be achieved in SL methods by using photopolymerizable materials that can also be selectively chemically modified during the SL part building process. This paper provides an update on our work directed at using combined photoradical initiated polymerization and photoacid generator based chemical modification of a polymeric scaffold via multi-wavelength SL to produce hierarchically structured scaffolds.

Three-dimensional profiles: a new tool to identify protein surface similarities

1998 ◽  
Vol 284 (4) ◽  
pp. 1211-1221 ◽  
Author(s):  
Manuel de Rinaldis ◽  
Gabriele Ausiello ◽  
Gianni Cesareni ◽  
Manuela Helmer-Citterich
Keyword(s):  
Three Dimensional ◽  
Protein Surface

Engineered Tissue Scaffolds With Variational Porous Architecture

2010 ◽  
Vol 133 (1) ◽  
Author(s):  
A. K. M. B. Khoda ◽  
Ibrahim T. Ozbolat ◽  
Bahattin Koc
Keyword(s):  
Tool Path ◽  
Three Dimensional ◽  
Tissue Scaffolds ◽  
Operation Technique ◽  
Layer By Layer ◽  
Functional Requirements ◽  
Channel Networks ◽  
Porous Architecture ◽  
Continuous Filament ◽  
Internal Architecture

This paper presents a novel computer-aided modeling of 3D tissue scaffolds with a controlled internal architecture. The complex internal architecture of scaffolds is biomimetically modeled with controlled micro-architecture to satisfy different and sometimes conflicting functional requirements. A functionally gradient porosity function is used to vary the porosity of the designed scaffolds spatially to mimic the functionality of tissues or organs. The three-dimensional porous structures of the scaffold are geometrically partition into functionally uniform porosity regions with a novel offsetting operation technique described in this paper. After determining the functionally uniform porous regions, an optimized deposition-path planning is presented to generate the variational internal porosity architecture with enhanced control of interconnected channel networks and continuous filament deposition. The presented methods are implemented, and illustrative examples are presented in this paper. Moreover, a sample optimized tool path for each example is fabricated layer-by-layer using a micronozzle biomaterial deposition system.

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