A role of ferritin heavy chain in opiate-induced neuropathology

2021 ◽  
Author(s):  
Anna Abt
Keyword(s):  
Heavy Chain ◽  
Ferritin Heavy Chain

scholarly journals TMIC-43. ROLE OF EXTRACELLULAR FERRITIN HEAVY CHAIN PROTEIN IN GLIOBLASTOMA MULTIFORME

2019 ◽  
Vol 21 (Supplement_6) ◽  
pp. vi257-vi257
Author(s):  
Bhavyata Pandya ◽  
Vagisha Ravi ◽  
James Connor
Keyword(s):  
Glioblastoma Multiforme ◽  
Heavy Chain ◽  
Dependent Manner ◽  
Cellular Viability ◽  
Ferritin Heavy Chain ◽  
Uptake Pathway ◽  
Iron Delivery ◽  
Human Oligodendrocytes ◽  
Dose Dependent

Abstract Increased expression of Ferritin heavy chain (FHC) protein has been associated with poor prognosis in Glioblastoma Multiforme (GBM) which is one of the most aggressive and common types of brain cancer. GBM patients have also been found to have increased extracellular ferritin levels, in their serum and cerebrospinal fluid (CSF), which are lowered once the source/tumor has been resected. Extracellular FHC can function as an iron delivery protein, and increasing amount of iron has been known to contribute to tumor initiation and proliferation. To study the effect of extracellular FHC in GBM cells we used patient derived GBM, CD133+ cancer stem cells (GSCs) from the pro-neural (T3691) and mesenchymal (T387) subtypes. Using recombinant FHC, conjugated with quantum dots (QD), we observed significant increase in cellular viability and intracellular uptake of FHC by the GSCs in a dose dependent manner. Our lab has previously shown that extracellular FHC interacts with T-Cell Immunoglobulin Mucin Receptor 1 (Tim-1) in the human oligodendrocytes. In order to determine if GSCs express the Tim-1 receptor we first confirmed its expression on GSCs using immunoblotting and immunocytochemistry. To test if FHC interacts with Tim-1, we performed knockdown of Tim-1 using siRNAs. However, the siRNA was not able to downregulate the Tim-1 receptors. Next, we exposed the GSCs to Sema4A, which has been shown in our previous studies to interact with Tim-1 receptor on human oligodendrocytes and is toxic to oligodendrocytes. The GSCs however were not affected by the saturable concentration of Sema4A. Thus, through this study we have shown the expression of potential FHC receptors on GSCs and a robust effect of H-ferritin on GSCs proliferation. Further experiments are warranted in this direction to understand this extracellular FHC uptake pathway and its role in GBM cell proliferation.

Evidence for a role of ferritin heavy chain in mediating reproductive processes of geese

2015 ◽  
Vol 15 (4) ◽  
pp. 205-209 ◽  
Author(s):  
Bo Kang ◽  
Dongmei Jiang ◽  
Rong Ma ◽  
Hui He
Keyword(s):  
Heavy Chain ◽  
Ferritin Heavy Chain ◽  
Reproductive Processes

scholarly journals Ferritin in Kidney and Vascular Related Diseases: Novel Roles for an Old Player

2019 ◽  
Vol 12 (2) ◽  
pp. 96 ◽  
Author(s):  
József Balla ◽  
György Balla ◽  
Abolfazl Zarjou
Keyword(s):  
Chronic Kidney Disease ◽  
Free Radicals ◽  
Kidney Disease ◽  
Heavy Chain ◽  
Biological Processes ◽  
Ferritin Heavy Chain ◽  
The Past ◽  
Cellular Iron ◽  
Iron Trafficking

Iron is at the forefront of a number of pivotal biological processes due to its ability to readily accept and donate electrons. However, this property may also catalyze the generation of free radicals with ensuing cellular and tissue toxicity. Accordingly, throughout evolution numerous pathways and proteins have evolved to minimize the potential hazardous effects of iron cations and yet allow for readily available iron cations in a wide variety of fundamental metabolic processes. One of the extensively studied proteins in the context of systemic and cellular iron metabolisms is ferritin. While clinicians utilize serum ferritin to monitor body iron stores and inflammation, it is important to note that the vast majority of ferritin is located intracellularly. Intracellular ferritin is made of two different subunits (heavy and light chain) and plays an imperative role as a safe iron depot. In the past couple of decades our understanding of ferritin biology has remarkably improved. Additionally, a significant body of evidence has emerged describing the significance of the kidney in iron trafficking and homeostasis. Here, we briefly discuss some of the most important findings that relate to the role of iron and ferritin heavy chain in the context of kidney-related diseases and, in particular, vascular calcification, which is a frequent complication of chronic kidney disease.

Higher CSF Ferritin Heavy-Chain (Fth1) and Transferrin Predict Better Neurocognitive Performance in People with HIV

2021 ◽  
Author(s):  
Harpreet Kaur ◽  
William S. Bush ◽  
Scott L. Letendre ◽  
Ronald J. Ellis ◽  
Robert K. Heaton ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Neurocognitive Performance ◽  
Ferritin Heavy Chain
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