The Effects of Macromolecular Crowding and System Volume on Protein Aggregation

2020 ◽  
Author(s):  
John Erik Bridstrup
Keyword(s):  
Protein Aggregation ◽  
Macromolecular Crowding ◽  
System Volume

scholarly journals Localization of Protein Aggregation in Escherichia coli Is Governed by Diffusion and Nucleoid Macromolecular Crowding Effect

2013 ◽  
Vol 9 (4) ◽  
pp. e1003038 ◽  
Author(s):  
Anne-Sophie Coquel ◽  
Jean-Pascal Jacob ◽  
Mael Primet ◽  
Alice Demarez ◽  
Mariella Dimiccoli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein Aggregation ◽  
Macromolecular Crowding ◽  
Crowding Effect

scholarly journals Investigating the effects of molecular crowding on the kinetics of protein aggregation

2020 ◽  
Author(s):  
John S. Schreck ◽  
John Bridstrup ◽  
Jian-Min Yuan
Keyword(s):  
Protein Aggregation ◽  
Kinetic Equations ◽  
Macromolecular Crowding ◽  
Molecular Crowding ◽  
Dynamic Effects ◽  
Reaction Step ◽  
Speed Up ◽  
Kinetics Of

The thermodynamics and kinetics of protein folding and protein aggregation in vivo are of great importance in numerous scientific areas including fundamental biophysics research, nanotechnology, and medicine. However, these processes remain poorly understood in both in vivo and in vitro systems. Here we extend an established model for protein aggregation that is based on the kinetic equations for the moments of the polymer size distribution by introducing macromolecular crowding particles into the model using scaled-particle and transition-state theories. The model predicts that the presence of crowders can either speed up, cause no change to, or slow down the progress of the aggregation compared to crowder-free solutions, in striking agreement with experimental results from nine different amyloid-forming proteins that utilized dextran as the crowder. These different dynamic effects of macromolecular crowding can be understood in terms of the change of excluded volume associated with each reaction step.

The effect of macromolecular crowding on protein aggregation and amyloid fibril formation

2004 ◽  
Vol 17 (5) ◽  
pp. 456-464 ◽  
Author(s):  
Larissa A. Munishkina ◽  
Elisa M. Cooper ◽  
Vladimir N. Uversky ◽  
Anthony L. Fink
Keyword(s):  
Protein Aggregation ◽  
Amyloid Fibril ◽  
Macromolecular Crowding ◽  
Fibril Formation ◽  
Amyloid Fibril Formation

scholarly journals Guiding Protein Aggregation with Macromolecular Crowding†

Biochemistry ◽  
2008 ◽  
Vol 47 (34) ◽  
pp. 8993-9006 ◽  
Author(s):  
Larissa A. Munishkina ◽  
Atta Ahmad ◽  
Anthony L. Fink ◽  
Vladimir N. Uversky
Keyword(s):  
Protein Aggregation ◽  
Macromolecular Crowding

An Ultra-Structural Study of Human Melanoma in Vivo and Vitro

1976 ◽  
Vol 34 ◽  
pp. 258-259
Author(s):  
James R. Gaylor ◽  
Fredda Schafer ◽  
Robert E. Nordquist
Keyword(s):  
Endoplasmic Reticulum ◽  
Golgi Apparatus ◽  
Protein Aggregation ◽  
Structural Study ◽  
Human Melanoma ◽  
Protein Matrix ◽  
Early Form ◽  
Endoplasmic Reticulum Protein ◽  
Mitochondrial Origin

Several theories on the origin of the melanosome exist. These include the Golgi origin theory, in which a tyrosinase-rich protein is "packaged" by the Golgi apparatus, thus forming the early form of the melanosome. A second theory postulates a mitochondrial origin of melanosomes. Its author contends that the melanosome is a modified mitochondria which acquires melanin during its development. A third theory states that a pre-melanosome is formed in the smooth or rough endoplasmic reticulum. Protein aggregation is suggested by one author as a possible source of the melanosome. This fourth theory postulates that the melanosome originates when the protein products of several genetic loci aggregate in the cytoplasm of the melanocyte. It is this protein matrix on which the melanin is deposited. It was with these theories in mind that this project was undertaken.

A novel P159L mutation in the Parkin gene related to autosomal dominantly inherited late onset parkinsonism and protein aggregation

2004 ◽  
Vol 31 (S 1) ◽  
Author(s):  
R Krüger ◽  
FP Marx ◽  
D Berg ◽  
C Holzmann ◽  
T Müller ◽  
...  
Keyword(s):  
Protein Aggregation ◽  
Late Onset ◽  
Parkin Gene
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