scholarly journals Fixings Agent Effect to β-carotene Stabilityof Crude Extract from Dunaliella salina Microalga at Different Temperature Condition

2017 ◽  
Vol 19 (3) ◽  
pp. 206
Author(s):  
Ardini Ria Oktora ◽  
Widodo Farid Ma’ruf ◽  
Tri Winarni Agustini
Keyword(s):  
Crude Extract ◽  
Research Method ◽  
High Stability ◽  
Dunaliella Salina ◽  
Ph Value ◽  
Storage Condition ◽  
Temperature Treatment ◽  
Pigment Degradation ◽  
Β Carotene ◽  
Carotene Degradation

Abstract<br />Dunaliella salina is a microalga rich pigment carotenoids that can be used asnatural dyes. The problem<br />faced for temporative β-carotene stability which is unstable and easily degraded is unstable and easily<br />degraded. The purpose ofthis researchwas to determine content and stability of β-carotene pigment in<br />D.salina with fixings addition of MgCO3 and NaHCO3 with temperature treatment as factor that influence<br />β-carotene pigment degradation during storage condition. The research method used β-carotene pigment<br />which was obtainedbymaceration and adding fixing agent (NaHCO3 0,5% and without fixings addition),<br />and was heated at temperature of (50°, 70° and 90°C), be measured the content of β-carotene pigment<br />during storage (observation hour 0, 24, 48, 72, 96, 120 and 144). Data analyzed by ANOVA and Honestly<br />Significant Different (HSD). The result showed that content of β-carotene pigment at 50°C temperature<br />showed that stability and pH value performed slowest degradation after144 hours. While stability and pH<br />value at 90°C temperature performed fastest degradation after 96 hours. Based on this study the addition of<br />NaHCO3 gave more effective in retaining β-carotene degradation compared to that of MgCO3 which showed<br />by high stability in sample NaHCO3 0,5%, temperature 50°C in 144 hours storage length.<br /><br />

scholarly journals Fixings Agent Effect to β-caroteneStabilityof Crude Extract from Dunaliella salinaMicroalgaat Different Temperature Condition

2016 ◽  
Vol 19 (3) ◽  
pp. 206
Author(s):  
Ardini Ria Oktora ◽  
Widodo Farid Ma'ruf ◽  
Tri Winarni Agustini
Keyword(s):  
Analysis Of Variance ◽  
Crude Extract ◽  
Research Method ◽  
High Stability ◽  
Ph Value ◽  
Storage Condition ◽  
Temperature Treatment ◽  
Pigment Degradation ◽  
Β Carotene ◽  
Carotene Degradation

faced for temporative β-carotene stability which is unstable and easily degraded is unstable and easily<br />degraded. The purpose ofthis researchwas to determine content and stability of β-carotene pigment in<br />D.salina with fixings addition of MgCO3 and NaHCO3 with temperature treatment as factor that influence<br />β-carotene pigment degradation during storage condition. The research method used β-carotene pigment<br />which was obtainedbymaceration and adding fixing agent (NaHCO3 0,5% and without fixings addition),<br />and was heated at temperature of (50°, 70° and 90°C), be measured the content of β-carotene pigment<br />during storage (observation hour 0, 24, 48, 72, 96, 120 and 144). Data were analyzed by analysis of variance<br />(ANOVA) and Honestly Significant Different (HSD). The result showed that content of β-carotene pigment<br />at 50°C temperature showed that stability and pH value performed slowest degradation after144 hours.<br />While stability and pH value at 90°C temperature performed fastest degradation after 96 hours. Based on<br />this study the addition of NaHCO3 gave more effective in retaining β-carotene degradation compared to that<br />of MgCO3 which showed by high stability in sample NaHCO3 0,5%, temperature 50°C in 144 hours storage<br />length.<br /><br />

scholarly journals Molecular and phylogenetic analysis reveals new diversity of Dunaliella salina from hypersaline environments

2021 ◽  
pp. 1-11
Author(s):  
Andrea Highfield ◽  
Angela Ward ◽  
Richard Pipe ◽  
Declan C. Schroeder
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Dunaliella Salina ◽  
Phylogenetic Analyses ◽  
Ssu Rdna ◽  
Lsu Rdna ◽  
Hypersaline Environments ◽  
Rdna Its ◽  
Β Carotene ◽  
Selection Of

Abstract Twelve hyper-β carotene-producing strains of algae assigned to the genus Dunaliella salina have been isolated from various hypersaline environments in Israel, South Africa, Namibia and Spain. Intron-sizing of the SSU rDNA and phylogenetic analysis of these isolates were undertaken using four commonly employed markers for genotyping, LSU rDNA, ITS, rbcL and tufA and their application to the study of Dunaliella evaluated. Novel isolates have been identified and phylogenetic analyses have shown the need for clarification on the taxonomy of Dunaliella salina. We propose the division of D. salina into four sub-clades as defined by a robust phylogeny based on the concatenation of four genes. This study further demonstrates the considerable genetic diversity within D. salina and the potential of genetic analyses for aiding in the selection of prospective economically important strains.

Effects of various environmental factors on β-carotene production by Dunaliella salina. —note—

1992 ◽  
Vol 73 (2) ◽  
pp. 178
Author(s):  
Yukiho Yamaoka ◽  
Osamu Takimura ◽  
Hiroyuki Fuse ◽  
Kazuo Kamimura ◽  
Eichi Manabe ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Dunaliella Salina ◽  
Β Carotene

PENGARUH EKSTRAK KASAR BUAH PALA MYRISTICA ARGENTEA TERHADAP PERTUMBUHAN IKAN KOI CYPRINUS CAPRIO

2021 ◽  
Vol 4 (1) ◽  
pp. 7-10
Author(s):  
Nuryanti Leko ◽  
Sutia Budi ◽  
Mardiana Mardiana ◽  
Dahlifa Dahlifa
Keyword(s):  
Growth Rate ◽  
Crude Extract ◽  
Research Method ◽  
Ornamental Fish ◽  
Good Effect ◽  
Length Growth ◽  
Weight Growth ◽  
Randomized Design ◽  
Test Parameters ◽  
Completely Randomized Design

Ikan koi di Indonesia merupakan ikan hias favorit dan banyak digemari masyarakat luas karena tubuhnya yang mempesona dan harganya relatif tidak terlalu mahal. Ikan koi sekarang ini masih menjadi salah satu komoditas perdagangan yang cukup baik dalam bidang perikanan. Tujuan dari penelitian ini adalah untuk mengetahui Pengaruh  Ekstrak Kasar Buah Pala Myristica Argentea  Terhadap Pertumbuhan Ikan Koi Cyprinus Caprio. Metode penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) ,dengan empat perlakuan dan tiga ulangan yaitu perlakuan A (Dosis 5% ekstrak kasar  buah pala), perlakuan B (Dosis 10% ekstrak kasar buah pala), perlakuan C (Dosis 15% ekstrak kasar buah pala) dan perlakuan D (   Dosis 0 % ekstrak kasar buah pala). Parameter uji berupa lajui pertumhuhan bobot dan pertumbuhan panjang. Hasil penelitian menunjukkan bahwa ekstrak Kasar Daging Buah Pala myristica argentea memberikan pengaruh yang baik terhadap pertumbuhan ikan ko setelah diberikan perlakuan.. Dosis yang baik untuk pertumbuhan berat dan panjang ikan koi ialah dosis  10% - 5%. Koi fish in Indonesia is a favorite ornamental fish and much-loved by the wider community because of its charming body and relatively inexpensive price. Koi fish is still one of the trade commodities that is quite good in the field of fisheries. The purpose of this study was to determine the Effect of Crude Extract of Nutmeg Myristica Argentea on the Growth of Cyprinus Caprio Koi Fish. The research method used was a completely randomized design (CRD), with four treatments and three replications, namely treatment A (5% dose of nutmeg crude extract), treatment B (10% dose of nutmeg crude extract), treatment C (15% dose of nutmeg extract). crude nutmeg) and treatment D (Dose of 0% crude extract of nutmeg). Test parameters in the form of weight growth rate and length growth. The results showed that the crude extract of the Nutmeg myristica argentea had a good effect on the growth of koi fish after being given treatment. A good dose for the growth of weight and length of koi fish is a dose of 10% - 5%

CRISPR/Cas9-induced β-carotene Hydroxylase Mutation in Dunaliella Salina CCAP19/18

2021 ◽  
Author(s):  
Lina Hu ◽  
Shu ying FENG ◽  
Gaofeng Liang ◽  
Jingxia Du ◽  
Aifang Li ◽  
...  
Keyword(s):  
High Performance ◽  
Dunaliella Salina ◽  
Gene Editing ◽  
Expression System ◽  
Transformation Method ◽  
Practical Significance ◽  
Exon 1 ◽  
Targeted Gene Editing ◽  
Foreign Genes ◽  
Β Carotene

Abstract Dunaliella salina (D. salina) has been exploited as a novel expression system for the field of genetic engineering. However, owing to the low or inconsistent expression of target proteins, it has been greatly restricted to practical production of recombinant proteins. Since the accurate gene editing function of CRISPR/Cas system, β-carotene hydroxylase gene was chosen as an example to explore D. salina application with the purpose of improving expression level of foreign genes. In this paper, based on pKSE401 backbone, three CRISPR/Cas9 binary vectors were constructed to targeting exon 1 and 3 of the β-carotene hydroxylase of D. salina CCAP19/18 (Dschyb). D. salina mutants were obtained by salt gradient transformation method, and the expression of Dschyb gene were identified through real-time fluorescent quantitative PCR. Moreover, carotenoids content was analyzed by high-performance liquid chromatography at different time points after high intensity treatment. Compared with wild type strains, the β-carotene levels of mutants showed a significant increase, nearly up to 1.4 μg/ml, and the levels of zeaxanthin decreased to various degrees in mutants. All the results provide a compelling evidence for targeted gene editing in D. salina. This study gave a first successful gene editing of D. salina which has a very important practical significance for increasing carotene yield and meeting realistic industry demand. Furthermore, it provides an approach to overcome the current obstacles of D. salina, and then gives a strong tool to facilitates the development and application of D. salina system.

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