scholarly journals The Use of Fresh Cogongrass as Transportation Media with Level Dry System for Broodstock of Crayfish on Various Old Time

2015 ◽  
Vol 17 (3) ◽  
pp. 197
Author(s):  
Ferdinand Hukama Taqwa ◽  
Yulisman - ◽  
I. S. Yulian
Keyword(s):  
Survival Rate ◽  
Low Temperature ◽  
Experimental Animal ◽  
Pond Water ◽  
Filler Material ◽  
Design Experiment ◽  
Level System ◽  
Major Research

The research was to determine the effect of the use of fresh cogongrass as media packaging filler on survival rate of broodstock of crayfish that transported with level dry system for 24, 48, dan 72 hours which randomized completely design experiment. The research preparation were media preparation of pond water, preparation of filler material and preparation of experimental animal (3 days adaptation after arrived from producer and starvation for 24 hours). The major research were anestetion of broodstock of crayfish with direct sock of low temperature at 12oC for ±5 minutes, transportation experiment of broodstock with fresh cogongrass as material filler. The result showed that the use of fresh cogongrass on various old time of dry level system has a significantly different to time of recovery and survival rate of broodstock of crayfish after tranforted. The fastest time of recovery was 89.55 seconds for 24 hours transportation and significantly different with time of transportation for 48 hours and 72 hours. The survival rate after tranported the highest was 98.89% for 24 hours transportation and significantly different with time of transportation for 48 hours and 72 hours. This research showed that the use of fresh cogongrass as material packaging filler of dry level system was still effective until 48 hours.<br />Keywords: afresh cogongrass, broodstock of crayfish, various old time transportation, level dry system

scholarly journals Infektivitas parasit Ichtyophthirius multifiliis yang disimpan pada suhu rendah

2016 ◽  
Vol 15 (2) ◽  
pp. 93
Author(s):  
, Rahman ◽  
, Sukenda ◽  
Sri Nuryati ◽  
Dendi Hidayatullah
Keyword(s):  
Survival Rate ◽  
Low Temperature ◽  
White Spot ◽  
White Spot Disease ◽  
Spot Disease ◽  
Lower Temperature ◽  
Poecilia Sphenops ◽  
Model Fish

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">The aim of this study was to evaluate infectivity of <em>Ichtyophthirius multifiliis</em> which caused white spot disease maintained at low temperature without its host. Briefly, the trophont stage of parasites were subjected at control (28 °C) and lower temperature (9 °C) for 14 consecutive days of observation. The rate of survival, and excystment of descendants were examined descriptively at the last day of observation. Here, the infectivity of parasite then performed by means infecting the model fish <em>Poecilia sphenops</em> (black moly) with escaping theronts. The results revealed that the survival rate and excystment  rate of parasite were decreased as maintaining period increased. The final rate of survival, and excystment of parasite were 35% and 33,3% respectively. Additionally, the descendants came out with high abnormality which recognized by weak mobility and lower infectivity (50%) compared to the control (80%). Then, it is concluded that, maintaining <em>I. multifiliis</em> at low temperature without its host for 14 consecutive days will decreased the infectivity.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: white spot, obligat parasite, excystment, infectivity</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong>ABSTRAK</strong><strong></strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Penelitian ini bertujuan untuk mengevaluasi infektivitas parasit <em>Ichtyophthirius multifiliis</em> penyebab penyakit bintik putih (<em>white spot</em>) yang dipelihara tanpa inang pada suhu rendah. Parasit dengan stadia trophont dipelihara pada suhu ruang (28 °C) dan suhu rendah (9 °C) selama 14 hari. Selama masa pemeliharaan tersebut tingkat kelulusan hidup, dan tingkat eksismen parasit diukur dan dibandingkan secara deskriptif. Hari terakhir pemeliharaan dilakukan uji tantang pada ikan <em>black moly</em> <em>Poecilia sphenops</em> untuk menilai infektivitas parasit. Hasil penelitian memperlihatkan bahwa tingkat kelulusan hidup dan eksismen parasit semakin menurun dengan bertambahnya masa pemeliharaan. Akhir pengamatan  kelangsungan hidup, dan nilai eksismen tersebut berturut-turut adalah 35% dan 33%. Parasit yang disimpan pada suhu rendah selama 14 hari memperlihatkan infektivitas yang lebih rendah (50%) dibandingkan dengan perlakuan kontrol (80%). Kesimpulannya, penyimpanan parasit <em>I. multifiliis</em> pada suhu rendah selama 14 hari dapat menurunkan infektivitas parasit pada inang.</p><p class="NoParagraphStyle"> </p>Kata kunci: bintik putih, parasit obligat, eksismen, infektivitas

scholarly journals A Novel Endoscopic Nasopharyngectomy Technique Using Low-Temperature Plasma Radiofrequency Ablation with Piece Resection In Resectable Locally Recurrent Nasopharyngeal Carcinoma: Operative Video and Technical Nuances

2021 ◽  
Author(s):  
Xiong Zou ◽  
Yi-Jun Hua ◽  
You-Ping Liu ◽  
Yu-Long Xie ◽  
Yan-Feng Ouyang ◽  
...  
Keyword(s):  
Radiofrequency Ablation ◽  
Survival Rate ◽  
Nasopharyngeal Carcinoma ◽  
Low Temperature ◽  
Operation Time ◽  
Low Temperature Plasma ◽  
Temperature Plasma ◽  
Free Survival ◽  
Recurrent Nasopharyngeal Carcinoma ◽  
Locally Recurrent

Abstract Objectives: Endoscopic nasopharyngectomy (ENPG) with en bloc resection has been well accepted in resectable locally recurrent nasopharyngeal carcinoma (rNPC), but it is difficult to master the technique and disseminate. We designed a novel method using low-temperature plasma radiofrequency ablation (LPRA) with piece resection and evaluated the survival benefit.Materials and Methods: A total of 56 resectable rNPC patients were retrospectively analyzed, and the following factors were assessed: ratio of R0 margin, overall survival rate (OS), local relapse-free survival rate (LRFS), and progression-free survival rate (PFS).Results: All surgeries were successfully performed without any severe postoperative complications or deaths. The median operation time was 130 minutes (range, 32–280 minutes), with little blood loss (median, 30 ml; range, 5–500 ml). The average number of hospital days postoperation was 3 days (range, 2–5 days). All cases (100.0%) had R0 margins. The nasopharyngeal defects were completely re-epithelialized in 53 (94.6%) patients. The 2-year OS, LRFS, and PFS rates of the entire cohort were 100%, 90.7% and 88.6%, respectively. Multivariate Cox proportional hazards model analysis indicated that cycles of radiotherapy were independent risk factors for LRFS (hazard ratio [HR], 16.607; 95% CI 1.638–168.417; P = 0.017) and PFS (HR, 7.424; 95% CI 1.190–46.320; P = 0.032).Conclusions: Radical endoscopic nasopharyngectomy is a novel, safe and relatively easier method for treating resectable rNPC by using low-temperature plasma radiofrequency ablation with piece resection. However, further data and longer follow-up time are needed to prove its efficacy.

scholarly journals Tetraploidy  enhances the cold-resistance in yellow kiwifruit (Actinidia chinensis)

2020 ◽  
Author(s):  
Yipei Li ◽  
Xiaozhen Liu ◽  
Zhou Wei ◽  
Zhimin Zhang ◽  
Wen Bian ◽  
...  
Keyword(s):  
Survival Rate ◽  
Low Temperature ◽  
Cold Tolerance ◽  
Regulatory Networks ◽  
Temperature Tolerance ◽  
Temperature Treatment ◽  
Actinidia Chinensis ◽  
Low Temperature Tolerance ◽  
Low Temperature Treatment ◽  
Cold Tolerant

Abstract Yellow kiwifruit ( Actinidia chinensis ) is highly susceptible to severe weather, such as low temperature and frost, which may affect the production in the coming year. And the cold-resistant mechanism of kiwifruit associated with gene regulation is poorly investigated. To botain cold-resistant germplam, to provide insight into the causes of differences in low temperature tolerance due to ploidy and to better understand cold-adaptive mechanisms in tetraploid kiwifruit, the diploid yellow kiwifruit ‘SWFU03’ and its tetraploid plantlets were subjected to cold-tolerant screening with L-hydroxyproline (L-Hyp) and low temperature, the selected ones were then analyzed by transcriptome data and confirmed by RT-qPCR. The results showed that the survival rate of tetraploid plants was 62.22% when treated with 8 mmol/L L-Hyp for 30 days, while all the diploid ones died. After treated with 0°C for 12 h, then at room temperature for seven days, the survival rate of tetraploid plantlets was 42.22%, while all diploidy died. Hence, cold tolerance of the tetraploid plantlets was stronger than that of the diploid genotypes. Using these two screening systems, 126 cold-resistant tetraploid tissue culture plantlets were obtained. A total of 1630 differentially expressed genes (DEGs) were identified, of which 619 were up-regulated and 1011 were down-regulated in the low temperature treatment goup. The DEGs enriched in the cold-tolerance related pathways mainly included plant hormone signal transduction, and starch and sucrose metabolism pathway. RT-qPCR analysis confirmed the expression levels of eight up-regulated genes in these pathways in the cold-resistant mutants. In conclusion, this study has identified cold-resistant yellow kiwifruit plantlets and cold-tolerance related genes. Moreover, the dataset got in this study advances our knowledge of the cold-adaptive genes in the regulatory networks and leads to understand the cold tolerance mechanisms in the tetraploid yellow kiwifruit.

PERTUMBUHAN, SINTASAN DAN PRODUKSI IKAN NILA MERAH (Oreochromis niloticus) YANG DIBERI KOMBINASI PAKAN KOMERSIL DAN AMPAS TAHU HASIL FERMENTASI

2018 ◽  
Vol 17 (3) ◽  
Author(s):  
Hidayat Suryanto Suwoyo ◽  
Sri Redjeki Hesti Mulyaningrum ◽  
Rachman Syah
Keyword(s):  
Water Quality ◽  
Survival Rate ◽  
Oreochromis Niloticus ◽  
Stocking Density ◽  
Pond Water ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Feed Conversion ◽  
Red Tilapia ◽  
Commercial Feed

The study aimed to evaluate the combination between commercial feed and fermented tofu waste feeding for growth, survival rate and production of red Tilapia Oreochromis niloticus in pond. The study was conducted in Experimental Pond Installation of Research Institute for Coastal Aquaculture, Maros, South Sulawesi, using eight ponds with 2,500 m2 in size. Red Tilapia fingerlings (±8 cm in length) were reared for 112 days with 1 individu/m2 of stocking density. The feeding treatments were of 100% commercial feed (A), 75% commercial feed + 25% fermented tofu waste (B), 50% commercial feed + 50% fermented tofu waste (C), and 25% commercial feed + 75% fermented tofu waste (D). Feeding frequency was given twice a day using concentration 10% of biomass weight in the first month, and decreased by 3% of biomass weight in the fourth month. Experimental was designed by a completely randomized designed consisted of four treatments and two replications for each treatment. Observed variables were growth, survival rate, feed conversion ratio, production, and water quality. Present study indicated that different levels of combination of commercial feed and fermented tofu waste had significant effect on growth (P<0.05), but did not have significant effect on survival rate, production, and feed conversion ratio (P>0.05). Feeding by combination of 75% commercial feed + 25% fermented tofu waste could be utilized optimally by Tilapia. During experiment, pond water quality was in conducive condition to support the growth and survival rate of red Tilapia.

scholarly journals Low-temperature culturing improves survival rate of tissue-engineered cardiac cell sheets

2018 ◽  
Vol 14 ◽  
pp. 89-97
Author(s):  
Katsuhisa Sakaguchi ◽  
Yuto Hinata ◽  
Yuki Kagawa ◽  
Kiyotaka Iwasaki ◽  
Satoshi Tsuneda ◽  
...  
Keyword(s):  
Survival Rate ◽  
Low Temperature ◽  
Cardiac Cell ◽  
Cell Sheets
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