scholarly journals Electrolites and Heavy Metals in Coelomic Fluid of Sea Urchin, Arbacia lixula from Adriatic Sea: Biochemical Approach to Ecotoxicological Study

Naše more ◽  
2019 ◽  
Vol 66 (2) ◽  
pp. 51-56
Author(s):  
Muhamed Fočak ◽  
◽  
Subha Džafić ◽  
Damir Suljević
Keyword(s):  
Heavy Metals ◽  
Sea Urchin ◽  
Adriatic Sea ◽  
Coelomic Fluid ◽  
Biochemical Approach ◽  
Ecotoxicological Study ◽  
Arbacia Lixula

scholarly journals Cell-Free Coelomic Fluid Extracts of the Sea Urchin Arbacia lixula Impair Mitochondrial Potential and Cell Cycle Distribution and Stimulate Reactive Oxygen Species Production and Autophagic Activity in Triple-Negative MDA-MB231 Breast Cancer Cells

2020 ◽  
Vol 8 (4) ◽  
pp. 261 ◽  
Author(s):  
Claudio Luparello ◽  
Debora Ragona ◽  
Dalia Maria Lucia Asaro ◽  
Valentina Lazzara ◽  
Federica Affranchi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Sea Urchin ◽  
Triple Negative ◽  
Water Soluble ◽  
Coelomic Fluid ◽  
Cell Cycle Distribution ◽  
Mitochondrial Potential ◽  
Autophagic Activity ◽  
Arbacia Lixula

Triple-negative breast cancer (TNBC) is a highly malignant tumor histotype which lacks effective targeted therapies, thereby being considered as the most aggressive form of breast carcinoma. To identify novel compounds which could counteract TNBC cell growth, we explored the in vitro effects of crude extracts and <10 kDa-filtered fractions of the coelomic fluid obtained from the sea urchin Arbacia lixula on TNBC MDA-MB231 cells. We examined cell viability, cycle distribution, apoptotic/autophagic activity, and mitochondrial polarization/cell redox status. Here, we report the first data demonstrating an anti-TNBC effect by A. lixula-derived coelomic fluid extracts. Thus, identification of the water-soluble bioactive component(s) contained in the extracts deserve(s) further investigation aimed to devise novel promising prevention and/or treatment agents effective against highly malignant breast tumors.

Differences in ion regulation in the sea urchins Lytechinus variegatus and Arbacia lixula (Echinodermata: Echinoidea)

2007 ◽  
Vol 87 (3) ◽  
pp. 769-775 ◽  
Author(s):  
Denilton Vidolin ◽  
Ivonete A. Santos-Gouvea ◽  
Carolina A. Freire
Keyword(s):  
Sea Urchin ◽  
Sea Urchins ◽  
Tap Water ◽  
Distinct Species ◽  
Coelomic Fluid ◽  
Lytechinus Variegatus ◽  
Ion Regulation ◽  
Magnesium Supplementation ◽  
Ionic Concentrations ◽  
Arbacia Lixula

The regular sea urchin Lytechinus variegatus, a species previously reported from areas of reduced salinities, and Arbacia lixula, a species unreported from diluted waters, were submitted to seawater dilution or seawater dilution in magnesium-supplemented waters. Seawater (35 psu) was either proportionally diluted with filtered dechlorinated tap water (30 psu, 25 psu), or diluted and supplemented with magnesium as MgCl2 (30+Mg, 25+Mg), up to full-strength seawater Mg2+ levels (35 psu, ~54 mM Mg2+). Magnesium supplementation was intended to verify the interfering effect of magnesium on osmo-ionic concentrations of the coelomic fluid (CF) of two ecologically distinct species of sea urchins. After 6 h in control (35 psu) or experimental seawater, CF samples were withdrawn by puncturing through the peristomial membrane. Coelomic fluid osmolality ([Osm]), and concentrations of ([Na+]), ([Cl-]), ([Mg2+]) and ([K+]) were measured for both species. Under all conditions, L. variegatus displayed higher CF osmolality, [Na+], and [K+] values than the water (and A. lixula). Comparatively, L. variegatus is designated as a‘hyper-conformer’, while A. lixula is an ‘iso-conformer’. The CF [Mg2+] showed no evidence of being controlled by either species. Mg2+ supplementation in diluted seawater affected Mg2+ and Cl- levels only. Na+ appears to be taken up actively by L. variegatus, rendering its CF mostly hyper-ionic for Na+ (and hyperosmotic) relative to external seawater. The different gradients observed with the different ions suggest selective permeabilities or ion regulation by L. variegatus.

scholarly journals Chemical characterization of red cells from the black sea urchin Arbacia lixula by X-ray photoelectron spectroscopy

RSC Advances ◽  
2021 ◽  
Vol 11 (43) ◽  
pp. 27074-27083
Author(s):  
Patrizia Pagliara ◽  
Daniela Chirizzi ◽  
Maria Rachele Guascito
Keyword(s):  
Immune Response ◽  
Sea Urchin ◽  
Photoelectron Spectroscopy ◽  
Chemical Characterization ◽  
Antimicrobial Activities ◽  
The Black Sea ◽  
Coelomic Fluid ◽  
X Ray ◽  
Arbacia Lixula

Red spherula cells from sea urchin coelomic fluid have attracted great interest for their specific and intriguing properties, such as antimicrobial activities and immune response, that probably tie in with their red characteristic pigments.

scholarly journals Antibiotic and metal resistance of cultivable bacteria in the Antarctic sea urchin

2016 ◽  
Vol 28 (4) ◽  
pp. 261-268 ◽  
Author(s):  
M. González-Aravena ◽  
R. Urtubia ◽  
K. Del Campo ◽  
P. Lavín ◽  
C.M.V.L. Wong ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Antibiotic Resistance ◽  
Sea Urchin ◽  
Sea Urchins ◽  
Antibiotic Resistance Genes ◽  
Metal Resistance ◽  
Coelomic Fluid ◽  
Bacterial Strains ◽  
Cultivable Bacteria ◽  
The Antarctic

AbstractIn this paper we report the first characterization of cultivable bacteria obtained from the Antarctic sea urchinSterechinus neumayeri. The coelomic fluid was obtained from a pool of sea urchins which was plated onto different media to isolate the bacteria. A total of 42 isolates of psychrotrophic and aerobic γ-Proteobacteria (59.5%), Flavobacteria (33.3%) and Actinomycetes (7.2%) were isolated and sequenced. These bacteria were exposed to heavy metals and antibiotics, where 38 strains were analysed by the minimal inhibitory concentration method. Antibiotic resistance was detected in 44% of cultivable strains, and a further 13% presented co-resistance to antibiotics and heavy metals. The genera of bacteria that showed an increased resistance and co-resistance to metals and antibiotics wereFlavobacterium,PsychrobacterandPseudomonas. Additionally, 30.9% of isolated bacterial strains contained plasmids, which are probably related to resistance and co-resistance to metals. These results indicate that sea urchin-associated bacteria could be reservoirs for antibiotic resistance genes.

scholarly journals Temperature modulates the response of the thermophilous sea urchin Arbacia lixula early life stages to CO2-driven acidification

2014 ◽  
Vol 93 ◽  
pp. 70-77 ◽  
Author(s):  
Paola Gianguzza ◽  
Giulia Visconti ◽  
Fabrizio Gianguzza ◽  
Salvatrice Vizzini ◽  
Gianluca Sarà ◽  
...  
Keyword(s):  
Sea Urchin ◽  
Early Life ◽  
Life Stages ◽  
Early Life Stages ◽  
Arbacia Lixula

scholarly journals CHANGES IN THE POTASSIUM CONTENT OF SEA URCHIN EGGS ON FERTILIZATION

1951 ◽  
Vol 34 (3) ◽  
pp. 285-293 ◽  
Author(s):  
Anna Monroy Oddo ◽  
Maria Esposito
Keyword(s):  
Sea Urchin ◽  
Sea Water ◽  
Paracentrotus Lividus ◽  
Potassium Content ◽  
Minimum Point ◽  
Sea Urchin Eggs ◽  
Maximum Loss ◽  
Arbacia Lixula

In the eggs of Arbacia lixula and Paracentrotus lividus an uptake of K occurs during the first 10 minutes following fertilization. Between 10 and 40 minutes K is then released. Both in Arbacia and in Paracentrotus the minimum point of the curve coincides with the nuclear streak stage. A maximum loss of 25 per cent in Arbacia and 20 per cent in Paracentrotus with respect to the amount present in the unfertilized eggs has been found. From 40 minutes up to 1 hour K undergoes a further increase and when the first cleavage sets in the same amount of K is present as in the unfertilized eggs. By treating the eggs with K-free artificial sea water it has been established that about 60 per cent of the K content of the eggs is in a non-diffusible condition. Also under such conditions the eggs when fertilized are able to take up even the very small amount of K present in the medium that was released by them prior to fertilization.

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