Application of pantohematogen dry for treatment of discirculatory disorders in chronic prostatitis (experimental and clinical study)

2021 ◽  
Vol 11 (1) ◽  
pp. 63-68
Author(s):  
Igor V. Tihonov ◽  
Igor I. Tityaev ◽  
Denis S. Kasyanov ◽  
Ramil H. Chekushin
Keyword(s):  
Clinical Study ◽  
Prostate Gland ◽  
Normal Structure ◽  
Treated Group ◽  
Laboratory Animals ◽  
Venous Stasis ◽  
Morphological Examination ◽  
Regional Lymph Nodes ◽  
Clinical Phase ◽  
Enteral Correction

PURPOSE: To study in experiment and in clinical conditions the effectiveness of the preparation pantogematogen dry, obtained from the blood of a maral, for the correction of dyscirculatory disorders in the prostate gland. MATERIALS AND METHODS: The experimental and morphological part of the work was performed on 90 male Wistar rats with local venous congestion in the small pelvis due to ligation of the internal iliac veins. Animals of the main group (n = 30) underwent enteral correction with dry pantohematogen (in a daily dose of 10 mg per 100 g of animal weight) starting from the 7th day after modeling the disease, the course was 14 days. The rats of the 2nd group (n = 30) after modeling of venous stasis were not treated. Group 3 consisted of intact animals. During the clinical phase of the study, 61 patients with chronic congestive prostatitis were treated. All patients received standard therapy, and patients in the treatment group (n = 30) additionally received highly dispersed aerosol transrectal irrigation with an aqueous solution of pantohematogen, daily, for a course of 10 procedures. RESULTS AND DISCUSSION: Morphological examination of the prostate gland of laboratory animals with experimental discirculatory prostatitis and its subsequent enteral correction with panthematogen dry indicated significant differences compared with animals of the 2nd group. A tendency towards restoration of the normal structure of the prostate, its metabolism, regional and organ blood and lymph outflow, improvement of the drainage-detoxification and transport activity of its regional lymph nodes was noted. In the clinical part of the study, the effectiveness of the use of pantogematogen in patients with chronic congestive prostatitis was shown. At the end of the course of treatment in patients of the 1st group, the disappearance of pain (in 72.4% of cases) and dysuric (in 86.2% of cases) syndromes was observed, and in the rest a decrease in their intensity. According to the TRUS of the prostate in patients of the 1st group, after treatment in 52% of cases, a decrease in the volume of the prostate was noted. CONCLUSIONS: The results of an experimental clinical study indicate the prospects for the inclusion of pantogematogen in the complex therapy of patients with chronic congestive prostatitis.

Osteonecrosis of the Femoral Head of Laboratory Animals: The Lessons Learned from a Comparative Study of Osteonecrosis in Man and Experimental Animals

2003 ◽  
Vol 40 (4) ◽  
pp. 345-354 ◽  
Author(s):  
J. H. Boss ◽  
I. Misselevich
Keyword(s):  
Blood Flow ◽  
Femoral Head ◽  
Weight Bearing ◽  
Arterial Occlusion ◽  
Lessons Learned ◽  
Laboratory Animals ◽  
Treatment Modalities ◽  
Venous Stasis ◽  
Spontaneously Hypertensive ◽  
Bearing Loads

Animal models of osteonecrosis of the femoral head are indispensable to the understanding of successful treatment modalities for avascular necrosis of the femoral head in adults and in children with Legg-Calvé-Perthes disease. Many of these models adequately reflect the current “vascular deprivation” theory regarding the etiology of the disease. In addition to spontaneous occurrence, surgical- and corticosteroid-induced models are suitable, common experimental ones. Osteonecrosis of spontaneously hypertensive rats appears to be due to defective bone formation and compression of the arteries entering the femoral head at its lateral facets by daily weight-bearing loads. Successful modeling of surgical-induced femoral capital necrosis can be a challenge in animals with a dual epiphyseal blood supply. High doses of corticosteroids are a pivotal risk factor in the development of osteonecrosis. The pathogenesis of corticosteroid-induced osteonecrosis likely resides in reduced blood flow. Steroids may reduce blood flow by numerous mechanisms, including marrow adipocytic hypertrophy leading to sinusoidal compression, venous stasis and, eventually, obstruction of the arteries, and arterial occlusion by fat emboli and lipid-loaded fibrin-platelet thrombi. Other, less common varieties of osteonecrosis include those secondary to endotoxin-induced disseminated intravascular coagulation, immune reactions, immoderately low or high temperatures, and high-impact-related injuries. Common to these diverse forms of osteonecrosis are fibrin thrombi clogging arterioles and small arteries.

scholarly journals α-Methylacyl-CoA racemase (AMACR) serves as a prognostic biomarker for the early recurrence/metastasis of HCC

2014 ◽  
Vol 67 (11) ◽  
pp. 974-979 ◽  
Author(s):  
Bo Xu ◽  
Zhixiong Cai ◽  
Yongyi Zeng ◽  
Lihong Chen ◽  
Xiaobo Du ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Clinical Study ◽  
Quantitative Proteomics ◽  
Large Scale ◽  
Prognostic Biomarker ◽  
Early Recurrence ◽  
Clinical Parameter ◽  
Regional Lymph Nodes ◽  
High Expression Group ◽  
Low Expression

AimsHepatocellular carcinoma (HCC) is one of the most common malignancies worldwide, and it is still lacking effective prognostic biomarkers so far. Previous results of the iTRAQ-based quantitative proteomics study (iTRAQ-2DLC-MS/MS) have shown that α-methylacyl-CoA racemase (AMACR) might be a promising prognostic biomarker for the early recurrence/metastasis of hepatocellular carcinoma (HCC). Here a large-scale cohort clinical study was performed to evaluate its prognostic potential.MethodsHCC samples from patients (n=158) were used for the construction of tissue microarray. The expression level of AMACR was determined by immunohistochemical staining. A large-scale cohort clinical study between the expression of AMACR and some major clinical parameter has been performed to assess the prognostic potential of AMACR for the early recurrence/metastasis of HCC.ResultsSome important clinical parameters such as α-fetoprotein, tumour numbers, dissemination to regional lymph nodes, tumour capsule and portal vein tumour thrombosis are significantly associated with the low expression of AMACR. The expression of AMACR was an independent factor for the survival of patients with HCC. The median survival time was 17 months in the low-expression group compared with 45 months in the high-expression group.ConclusionsThis study reveals that the AMACR might be a potential prognostic marker for predicting early recurrence/metastasis of HCC after hepatectomy.

Assessment of efficiency of complex treatment of soft tissue wounds by means of oxytocin and titanium aquacomplex glycerosolvate in experiment

10.12737/4115 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 1-4
Author(s):  
Глухов ◽  
A. Glukhov ◽  
Мохова ◽  
Olesya Mokhova ◽  
Микулич ◽  
...  
Keyword(s):  
Clinical Study ◽  
Soft Tissue ◽  
Granulation Tissue ◽  
Inflammatory Cells ◽  
Male Rats ◽  
Laboratory Animals ◽  
Complex Treatment ◽  
High Efficacy ◽  
Combined Use ◽  
White Mongrel

Purpose of the study is to assess the efficiency use of oxytocin and titanium aquacomplex glycerosol-vate in the treatment of soft tissue wounds. The study was carried out on 72 white mongrel male rats weighing 210 ± 10 g with simulated aseptic wounds. The sampling was carried out for 1, 3, 5 and 7 days from the beginning of the experiment. To assess the course of wound process in the clinical study, planimetric and histological methods were applied. The comparison of obtained results has allowed to reveal the high efficacy of combined use of oxytocin and glycerosolvate titanium. On the basis of clinical and planimetric methods of investigation it was established that reduction of the area of the wound is significantly faster in animals treated with the use of oxytocin and glycerosolvate titanium. This treatment has a stimulating effect on the activity of connective tissue - accelerates the appearance and maturation of granulation tissue, occur earlier replacement of a number of inflammatory cells into cells, which provide proliferation; earlier appearance of collagen fibers and the prevalence among them horizontally. The use of oxytocin and titanium aquacomplex glycerosolvate in laboratory animals with aseptic wounds influences positively on the dynamic of wounded process, allows to accelerate the appearance of granulation tissue and epithelialization.

Assessment of quality and clinical similarity (pharmacokinetic and safety) of HD204, a biosimilar of bevacizumab.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e21556-e21556
Author(s):  
Jocelyn Hii ◽  
Martin Demarchi ◽  
Pierre Coliat ◽  
Michael J Kim ◽  
Kuo Wei Chan ◽  
...  
Keyword(s):  
Clinical Study ◽  
Cancer Progression ◽  
Neutralizing Antibodies ◽  
Volume Of Distribution ◽  
Prospective Clinical Study ◽  
Healthy Male ◽  
Clinical Phase ◽  
Humanized Monoclonal Antibody ◽  
The Impact ◽  
Male Subjects

e21556 Background: Prestige Biopharma Pte Ltd is developing HD204, a biosimilar candidate of Bevacizumab (Avastin). Bevacizumab has been approved in the treatment of a variety of metastatic tumours. Bevacizumab, a recombinant humanized monoclonal antibody block angiogenesis which is required for cancer progression by preventing binding of soluble vascular endothelial growth factor (VEGF) to VEGF receptors. Due to heterogeneity nature of antibody therapeutic, the impact on quality of HD204 on safety and pharmacokinetic (PK) was reaffirmed through clinical study to establish clinical similarity between HD204 and Avastin. Methods: Quality attributes identified to influence PK and safety established through comprehensive analytical characterization was used to correlate any potential differences (structural or biological) between the two compounds if any, could result in any clinical meaningful differences in safety and PK in the clinical settings. The PK and safety equivalence of HD204 relative to Avastin was demonstrated in a randomized, single-blind, single-dose, three-arm and parallel-group study clinical Phase I (SAMSON). A total of 120 healthy male subjects randomized 1:1:1 were to receive 1 mg/kg intravenous infusion of either HD204, EU- or US-Avastin. Various PK parameters, safety assessments not limiting to adverse events (AE) and measurement of antidrug antibodies (ADA) and neutralizing antibodies (NAb) were evaluated. Results: The pairwise comparisons of Exposure (AUC0-inf and AUC0-last), maximal concentration (Cmax) established equivalence between the 3 arms. All other PK parameters including half-life, clearance and volume of distribution were comparable between HD204 and Avastin treatment groups. Treatment related TEAEs reported for each group were 25.0%, 30.0% and 25.6% respectively and comparable. There were no treatment-emergent SAEs. Furthermore, none of the subjects treated with HD204 was ADA positive. Conclusions: HD204 demonstrated equivalent PK and safety profile to both US-Avastin and EU-Avastin at 1mg/kg administered as a 90-minute IV infusion to healthy male subjects. A prospective clinical study aimed to demonstrate equivalence in terms of efficacy, PK and safety is currently ongoing. Clinical trial information: 2017-005174-19.

Actions of oestradiol and progesterone on the prostate in female gerbils: reversal of the histological effects of castration

2014 ◽  
Vol 26 (4) ◽  
pp. 540 ◽  
Author(s):  
Marianna Zanatelli ◽  
Diego A. L. Silva ◽  
Filipe Z. Shinohara ◽  
Rejane M. Góes ◽  
Fernanda C. A. Santos ◽  
...  
Keyword(s):  
Mammalian Species ◽  
Prostate Gland ◽  
Secretory Activity ◽  
Hormone Treatment ◽  
Treated Group ◽  
Mineral Oil ◽  
Serum Concentrations ◽  
Prolonged Administration ◽  
Female Prostate ◽  
Active Gland

The female prostate is a functionally active gland in several mammalian species, including humans and rodents. Investigations of prostate morphophysiology during the phases of the oestrous cycle have shown that the female prostate is influenced by fluctuations in serum concentrations of oestradiol (E2) and progesterone (P4). The aim of the present study was to evaluate the effect of combined prolonged administration of E2 and P4 on the prostate in ovariectomised female gerbils. Ovariectomy caused atrophy and decreased glandular secretory activity. Administration of E2 and P4 (0.1 mg kg–1 diluted in 0.1 mL of mineral oil, every 48 h over 30 days) resulted in a recovery of overall prostate structure, as evidenced by increased epithelial height, mass and prostatic secretory activity, without leading the appearance of significant lesions. Evaluation of androgen receptor (AR) expression revealed increased immunoreactivity in the E2+P4-treated group. Immunostaining for oestrogen receptor (ER) α was decreased in the castrated groups, but increased in the group subjected to hormone treatment. There were no significant differences in ERβ immunoreactivity among the groups. Assessment of cell proliferation revealed greater immunoreactivity in the treated group. Together, the results indicate that the interaction between E2 and P4 may be responsible for maintaining female prostate gland histophysiology.

scholarly journals The effect of the phenolic and other active compounds of the alcohol and acid extracts of cinnamon on some of the Biochemical Parameters of induced diabetes mellitus

2015 ◽  
Vol 9 (1) ◽  
pp. 24-36
Author(s):  
Hayder Turki AL-Musaw AL-Musawi ◽  
Mohammed Ibrahem AL-Tai AL-Tai ◽  
Muna Turki AL-Musawi AL-Musawi
Keyword(s):  
Body Weight ◽  
Density Lipoprotein ◽  
Treated Group ◽  
Laboratory Animals ◽  
Control Group ◽  
Alcoholic Extract ◽  
Serum Concentrations ◽  
Active Compounds ◽  
Cinnamon Extract ◽  
Long Duration

The phenolic compound and other active compounds were extracted from cinnamon, in anattempt to produce a drug from Medical and herbal Plants for the diabetes on rats. Theextraction process included two methods the first carrid out with 2% v/v acetic acid andpropanol, and in the second process 70% v/v ethanol and chloroform were used. In the detectionof extracts it was noticed that both conteined phenols, flavonoids, tannins and Glycosides andCoumarins, while the saponin showed up only in the alcoholic extract of cinnamon. The phenoliccompounds in the extracts of cinnamon were determined by using Ultraviolet Spectroscopy (UV)and other function groups such as phenols, aldehyedes, estars, hydrocarbons, alcohols andcarboxylic compounds were diagnosed by using Infrared spectroscopy (IR). The study of the halflethal dose (midl lethal) (LD50) was also examined by testing the toxicity of the two cinnamon 2015 مجلة مركز بحوث التقنيات االحيائية المجلد التاسع- العدد االول42extracts. Rats were used as laboratory animals in conducting the experiments. The rats were fedby the dose (100, 200, 300,400, 500 mg / kg of body weight). The results have shown that there isno toxic effect in the cinnamon extracts. Similar concentrations were used to determine theeffective dose of the extracts in reducing the level of glucose in the blood of healthy animals. Thedose (300) mg / kg of body weight of the animal body has proved to be the the most efficient as itdecreased. The fasting glucose (Glu) serum consider by following of the cinnamon extracts withthe adopted groups during the duration of (30) days in comparison with the control group (theinfected) and outperformed the treated given Glibenelinide (daonil) group. The biochemical testsshowed that the serum concentrations of each of cholesterol (TC),Triglyceride (TG), low densitylipoprotein (LDL-C) was lowered in each of the groups treated with both cinnamon extracts incomparison with the positive groups (infected) throughout the duration (30) days and overcomethe daonil treated group. However the serum high density lipoprotein (HDL-C) level wasincreased in cinnamon extract treated groups and decreased in the control group (infected).The serum concentrations of enzymes (GOT) and (GPT) were measured to test the liver functionafter where their levels were lowered after (30) days in the cinnamon extracts administeredgroups compared with the two other groups, the control (positive) and the Daonil. The Urea andCreatinine levels were measured to determine or test the kidney functions, where it was observedas from the early days of the infection in that animals infected by induced diabetes developedsymptoms of the illness, apparent in the high levels of urine which affected the functions ofkidney, but the long duration (30 days) of treatment with these extracts of cinnamon proved theirefficiency over the control group (positive).

Cell biological studies of primary and metastatic tumors of the rat prostate

1990 ◽  
Vol 48 (3) ◽  
pp. 304-305
Author(s):  
Paul M. Heidger ◽  
William J. Kutschke ◽  
Ziad Bataineh
Keyword(s):  
Extracellular Matrix ◽  
Prostate Gland ◽  
Lung Parenchyma ◽  
Morphological Examination ◽  
Electron Microscopic ◽  
Biological Studies ◽  
Extracellular Matrix Components ◽  
Microscopic Studies ◽  
Dunning Tumor ◽  
Basic Studies

The widespread occurrence and high mortality rate in older men associated with disseminated adenocarcinoma of the prostate provide a strong impetus for basic studies of the normal and neoplastic prostate gland. The present studies have been undertaken with the objective of gaining a fuller appreciation of histo- and cytologic interrelationships between metaplastic prostatic tumors and their host organs.The Dunning rat adenocarcinoma has provided a model for some years for the study of primary prostatic tumors, and recently a tumor subline has been identified which preferentially metastasizes to lung (Dr. David M. Lubaroff, U. IA). Cells of the metastatic subline colonize lung following intravenous injection, and produce widespread lesions within the lung parenchyma, and deep to the visceral pleura, in a matter of weeks. Correlative light microscopic (LM), transmission (TEM) and scanning (SEM) electron microscopic studies have been conducted of both the primary Dunning tumor, and the metastatic lesions within lung, using routine preparative techniques. Morphological examination has been conducted in concert with immunocytochemical localization of components of the extracellular matrix, within both lung and tumor, and with studies of the growth and attachment characteristics of tumor cells cultured in wells coated with such extracellular matrix components as fibronectin, laminin, and collagen types I and IV.

Effects of Icariin on Histomorphometric Changes of Testis and Prostate Induced by Acrylamide in Mice

2020 ◽  
Author(s):  
Mahsa Doctor Arastoye Marandi ◽  
Maryam Yadegari ◽  
Abbas Shahedi ◽  
Majid Pourentezari ◽  
Morteza Anvari ◽  
...  
Keyword(s):  
Leydig Cells ◽  
Sham Group ◽  
Prostate Gland ◽  
Treated Group ◽  
Control Group ◽  
Testis Weight ◽  
Histopathological Changes ◽  
Histological Changes ◽  
The Mean ◽  
Control Sham

Background and Aims: This study aimed to observe the effect of Icariin on histomorphometric changes of testis and prostate induced by Acrylamide. Materials and Methods: Male mice were divided into four groups (n=8): A is the control group and does not get any treatment, B is the sham group and only received drinking water. C group received Acrylamide 10 mg/kg. D group received Acrylamide 15 mg/kg+1.5 mg/kg of Icariin. Histological changes in testis and prostate were examined using stereological methods. Results: Results showed decreases in testis weight of the group treated by (p≤0.01) and the group cured by Acrylamide +Icariin group (p≤0.05). The total volume of testis showed a reduction in the Acrylamide  group compared to other groups (p≤0.05). The total number of spermatogonia and spermatocyte cells in the Acrylamide group showed a decrease in comparison with the other groups (p≤0.05). The total number of spermatid cells in the Acrylamide group indicated a significant reduction in comparison with the control and sham group (p≤0.05). The total number of sertoli cells in the Acrylamide group showed a reduction when the number of leydig cells in the Acrylamide group showed a significant decrease in comparison with the control, sham, and Acrylamide+Icariin groups (p≤0.05). The mean Johnsen score was decreased in the Acrylamide treated group compared to control, sham, and Acrylamide+Icariin groups (p≤0.05). Testosterone concentration in the Acrylamide group showed a reduction in comparison with control, sham, and Acrylamide+Icariin groups (p≤0.05). Conclusion: Results demonstrated that Acrylamide altered the structure of the testis, prostate gland, and spermatogenesis stage, and Icariin treatment improved these histopathological changes.

Controlled Clinical Study with Fentiazac Suspension in Painful Inflammatory Diseases of Children

1983 ◽  
Vol 11 (5) ◽  
pp. 298-302 ◽  
Author(s):  
A Catti
Keyword(s):  
Clinical Study ◽  
Inflammatory Diseases ◽  
Signs And Symptoms ◽  
Treated Group ◽  
Test Drug ◽  
Double Blind Trial ◽  
Double Blind ◽  
Inflammatory Conditions ◽  
Objective Evidence ◽  
Controlled Clinical Study

In a parallel double-blind trial with fifty paediatric patients, the anti-inflammatory, anti-exudative and antipyretic effects of Fentiazac suspension were compared with Benzidamine drops in the treatment of painful inflammatory conditions. Both drugs proved to be effective in relieving the signs and symptoms considered, though Fentiazac was shown to be more rapid and active. Body temperature was lowered by both drugs. Patients treated with Fentiazac suspension had a normal temperature after 3 days of treatment whereas in the Benzidamine-treated group the same result was reached only after 4 days (p < 0·01). There was no subjective or objective evidence of local and/or systemic intolerance to either test drug.

scholarly journals Assessment of Histopathological Changes in the Liver and Spleen of Mice Infected with Salmonella typhi Following Treatment with Ciprofloxacin

2021 ◽  
pp. 761-768
Author(s):  
Nabeel Ahmed Rajab ◽  
Ahmed Mohammed Turki
Keyword(s):  
Histopathological Examination ◽  
Phosphate Buffer Solution ◽  
Normal Structure ◽  
Treated Group ◽  
Negative Control ◽  
Cell Swelling ◽  
Control Group ◽  
White Pulp ◽  
Buffer Solution ◽  
Sterile Phosphate Buffer

This study evaluated the toxicity of ciprofloxacin to spleen and liver when used for the treatment of mice infected with S. typhi for seven days. The dose concentration used in these experiments was 100mg/kg. Mice were divided into two groups . The first group (negative control) was not given ciprofloxacin, but rather a sterile phosphate buffer solution (PBS) as an alternative. Ciprofloxacin was administered to the second group. After seven days , the animals were sacrificed and organs (liver and spleen) were collected . The histopathological examination showed normal hepatocytes in the liver  and normal structure of  spleen cells in animals of control group . However, the treated group showed dilated and congested blood vessels with perivascular inflammatory cell cuffing and acute cell swelling  in the liver, as well as white pulp activation with an increased number of megakaryocyte cells in the spleen. Therefore, the current study suggests that  the concentration of 100 mg/kg of ciprofloxacin is considered to be toxic to  hepatocytes and splenocytes of mice  during the treatment period.

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