Possibilities of in vivo validation of a model of experimental osteoporosis

2020 ◽  
Vol 18 (4) ◽  
Author(s):  
Alekber A. Bairamov ◽  
Nailya Sh. Mamina ◽  
Tatiana L. Karonova ◽  
Petr D. Shabanov
Keyword(s):  
Bone Tissue ◽  
Experimental Model ◽  
X Ray ◽  
Experimental Osteoporosis ◽  
In Vivo Validation

In this work, the possibilities ofin vivovalidation of an experimental model of osteoporosis are analyzed. Themodel is based on the application of biochemical methodsforanalyzing predictors of osteoporosis inbloodserum,andtheirinformative value in the comparativeanalysis of the assessment of the degree of osteoporosisbasedoninstrumental studies of autopsy of bone tissue usingatomicabsorption spectroscopy and x-ray densitometry.

Possibilities of in vivo validation of a model of experimental osteoporosis

2020 ◽  
Vol 18 (4) ◽  
pp. 365-367
Author(s):  
Alekber A. Bairamov ◽  
Nailya Sh. Mamina ◽  
Tatiana L. Karonova ◽  
Petr D. Shabanov
Keyword(s):  
Bone Tissue ◽  
Experimental Model ◽  
X Ray ◽  
Experimental Osteoporosis ◽  
In Vivo Validation

In this work, the possibilities ofin vivovalidation of an experimental model of osteoporosis are analyzed. Themodel is based on the application of biochemical methodsforanalyzing predictors of osteoporosis inbloodserum,andtheirinformative value in the comparativeanalysis of the assessment of the degree of osteoporosisbasedoninstrumental studies of autopsy of bone tissue usingatomicabsorption spectroscopy and x-ray densitometry.

scholarly journals Surface-Modified Poly(l-lactide-co-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits

2020 ◽  
Vol 21 (20) ◽  
pp. 7541
Author(s):  
Małgorzata Krok-Borkowicz ◽  
Katarzyna Reczyńska ◽  
Łucja Rumian ◽  
Elżbieta Menaszek ◽  
Maciej Orzelski ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Photoelectron Spectroscopy ◽  
Sessile Drop ◽  
Bone Tissue Regeneration ◽  
In Vivo Studies ◽  
In Vivo Model ◽  
Type I ◽  
X Ray

Poly(l-lactide-co-glycolide) (PLGA) porous scaffolds were modified with collagen type I (PLGA/coll) or hydroxyapatite (PLGA/HAp) and implanted in rabbits osteochondral defects to check their biocompatibility and bone tissue regeneration potential. The scaffolds were fabricated using solvent casting/particulate leaching method. Their total porosity was 85% and the pore size was in the range of 250–320 µm. The physico-chemical properties of the scaffolds were evaluated using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), sessile drop, and compression tests. Three types of the scaffolds (unmodified PLGA, PLGA/coll, and PLGA/HAp) were implanted into the defects created in New Zealand rabbit femoral trochlears; empty defect acted as control. Samples were extracted after 1, 4, 12, and 26 weeks from the implantation, evaluated using micro-computed tomography (µCT), and stained by Masson–Goldner and hematoxylin-eosin. The results showed that the proposed method is suitable for fabrication of highly porous PLGA scaffolds. Effective deposition of both coll and HAp was confirmed on all surfaces of the pores through the entire scaffold volume. In the in vivo model, PLGA and PLGA/HAp scaffolds enhanced tissue ingrowth as shown by histological and morphometric analyses. Bone formation was the highest for PLGA/HAp scaffolds as evidenced by µCT. Neo-tissue formation in the defect site was well correlated with degradation kinetics of the scaffold material. Interestingly, around PLGA/coll extensive inflammation and inhibited tissue healing were detected, presumably due to immunological response of the host towards collagen of bovine origin. To summarize, PLGA scaffolds modified with HAp are the most promising materials for bone tissue regeneration.

Noninvasive Monitoring of Local Drug Release Using X-ray Ccomputed Tomography: Optimization and In Vitro/In Vivo Validation

2003 ◽  
Vol 92 (2) ◽  
pp. 289-296 ◽  
Author(s):  
Agata Szymanski-Exner ◽  
Nicholas T. Stowe ◽  
Kyle Salem ◽  
Roee Lazebnik ◽  
John R. Haaga ◽  
...  
Keyword(s):  
Drug Release ◽  
Noninvasive Monitoring ◽  
X Ray ◽  
In Vivo Validation

Preservation of Bone Tissue Quality during the Usage of Synthetic Bioactive Calcium Phosphate Mineral Coating for Prevention of Metallic Construction Migration

2020 ◽  
Vol 46 ◽  
pp. 67-74
Author(s):  
Alexander Markov ◽  
Andrey Ponomarev ◽  
Mikhail Zavatskij ◽  
Ksenya Stepanova ◽  
Sergej Krylov ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Tissue ◽  
Titanium Implants ◽  
Mineral Density ◽  
Tissue Quality ◽  
Implant Surface ◽  
X Ray ◽  
Phosphate Mineral ◽  
Negative Effect

This work is devoted to studying the reaction of bone tissue to installed titanium implants, which are bioinert in comparison with other metals used in traumatological and orthopedic practice, but which have a negative effect on bone tissue in the implant-to-bone area. In order to increase the affinity of the implant surface with bone tissue, it was proposed to use synthetic bioactive calcium phosphate mineral coating by applying it on titanium implants, which ultimately will make it possible to maintain the quality of the bone tissue around the implant after osteosynthesis or prosthetics. During the comparative research an in vivo experiment was conducted in which quality change of peri-implant zone of bone tissue when using titanium implants with synthetic bioactive calcium phosphate mineral coating and titanium implants without bioactive coating was analyzed. To analyze the results of the study, we used the X-ray computed tomography method, for a detailed assessment of the X-ray density and bone mineral density of the peri-implant zone. According to the results of the experiment, it was concluded that the reaction of bone tissue to synthetic bioactive calcium phosphate mineral coating of titanium implants was reduced, what ensures the preservation of bone tissue quality in the postoperative period at a high level.

Effects of vanadium (V) and magnesium (Mg) on rat bone tissue: mineral status and micromorphology. Consequences of V–Mg interactions

Metallomics ◽  
2014 ◽  
Vol 6 (12) ◽  
pp. 2260-2278 ◽  
Author(s):  
Agnieszka Ścibior ◽  
Agnieszka Adamczyk ◽  
Robert Mroczka ◽  
Irmina Niedźwiecka ◽  
Dorota Gołębiowska ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Experimental Model ◽  
Bone Mineral ◽  
Mineral Status ◽  
Bone Mineral Status ◽  
Rat Bone

Effects of vanadium and magnesium on bone mineral status and micromorphology were shown in anin vivoexperimental model.

scholarly journals Correction of bone remodeling in experimental osteoporosis

2020 ◽  
Vol 17 (4) ◽  
pp. 43-50
Author(s):  
Alekber A. Bairamov ◽  
Eugenii I. Maevsky ◽  
Petr D. Shabanov
Keyword(s):  
Succinic Acid ◽  
Atomic Absorption ◽  
Bone Tissue ◽  
Experimental Model ◽  
Experimental Work ◽  
Atomic Absorption Spectroscopy ◽  
High Efficiency ◽  
The Body ◽  
Excess Calcium ◽  
Experimental Osteoporosis

The experimental work analyzes the evaluation of the pharmacological efficacy of 3 experimental samples of the anti-osteoporosic preparation, created on the basis of salts of succinic acid in comparison with the comparison drug. On the experimental model of osteoporosis, according to the application of atomic absorption spectroscopy and flame photometry of the femur, the high efficiency of the new drug in the correction of induced pathology has been proved. The data obtained indicate that when acid salts of the natural conformer of succinic acid are used, it is possible to achieve sufficient saturation of bone tissue with calcium without overloading the body with excess calcium.

Preparation of cultured astrocytes for x-ray microanalysis

1989 ◽  
Vol 47 ◽  
pp. 988-989
Author(s):  
N.K.R. Smith ◽  
K.E. Hunter ◽  
P. Mobley ◽  
L.P. Felpel
Keyword(s):  
Cultured Cells ◽  
Elemental Content ◽  
Elemental Distribution ◽  
Sprague Dawley ◽  
X Ray ◽  
Cultured Astrocytes ◽  
Freeze Dried ◽  
Ultimate Objective

Electron probe energy dispersive x-ray microanalysis (XRMA) offers a powerful tool for the determination of intracellular elemental content of biological tissue. However, preparation of the tissue specimen , particularly excitable central nervous system (CNS) tissue , for XRMA is rather difficult, as dissection of a sample from the intact organism frequently results in artefacts in elemental distribution. To circumvent the problems inherent in the in vivo preparation, we turned to an in vitro preparation of astrocytes grown in tissue culture. However, preparations of in vitro samples offer a new and unique set of problems. Generally, cultured cells, growing in monolayer, must be harvested by either mechanical or enzymatic procedures, resulting in variable degrees of damage to the cells and compromised intracel1ular elemental distribution. The ultimate objective is to process and analyze unperturbed cells. With the objective of sparing others from some of the same efforts, we are reporting the considerable difficulties we have encountered in attempting to prepare astrocytes for XRMA.Tissue cultures of astrocytes from newborn C57 mice or Sprague Dawley rats were prepared and cultured by standard techniques, usually in T25 flasks, except as noted differently on Cytodex beads or on gelatin. After different preparative procedures, all samples were frozen on brass pins in liquid propane, stored in liquid nitrogen, cryosectioned (0.1 μm), freeze dried, and microanalyzed as previously reported.

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