scholarly journals Types of vegetative reactions in healthy people at different level of Na+/Li+ countertransport in erythrocyte membranes

1998 ◽  
Vol XXX (1-2) ◽  
pp. 26-35
Author(s):  
D. R. Khasanova
Keyword(s):  
Functional Status ◽  
Cell Membranes ◽  
Healthy People ◽  
Erythrocyte Membranes ◽  
Genetically Determined

Types of vegetative responding were studied with evaluation of initial vegetative reactions and vegetative activity in healthy people, aged from 7-42, in association with one of the markers of genetically determined structural and functional status of cell membranes - velocity of Na+/Li+ countransport in erythrocyte membranes. It was found that level of intensity of vegetative effects with transition of adaptation reactions to orthostasis is most characterestic of high values of transmembrane monotransport velocity.

Impermeability of rabbit erythrocytes to prostaglandins

1975 ◽  
Vol 229 (6) ◽  
pp. 1580-1584 ◽  
Author(s):  
LZ Bito ◽  
RA Baroody
Keyword(s):  
Fatty Acids ◽  
Cell Membranes ◽  
Erythrocyte Membranes ◽  
Rabbit Erythrocyte ◽  
The Mean ◽  
Intracellular Volume ◽  
Distribution Spaces ◽  
Apparent Distribution ◽  
Initial Uptake

Washed rabbit erythrocytes were suspended in Tris-electrolyte buffer containing [3H]prostaglandin (PG) E1, F1alpha, F2alpha, or A1 and one 14C-labeled compound such as sucrose. After up to 24 h of incubation, aliquots of centrifuged, packed cells and supernatant were oxidized and the 3H and 14C samples were counted. The mean sucrose space of the packed cell was 7.4%. After 1 min the E1, F1alpha, and F2alpha spaces were 16, 15.4, and 10.0%, respectively, and showed no increase even after 24 h of incubation at either 23 or 5 degrees C. At 23 degrees C the initial (0.5 min) PGA1 and thiourea spaces were 94 and 75%, respectively, whence the PGA1, but not the thiourea, space declined, reaching 30% at 4 h. The large initial uptake of PGA1 was eliminated at 5 degrees C, while it was accentuated at pH 6.8 or at a PGA1, concentration of 10(-3) M. 14C-Labeled arachidonic, octanoic, and other non-PG fatty acids gave apparent distribution spaces of 140-300%. These results show that PG's can partition into rabbit erythrocyte membranes, but the intracellular volume of the erythrocytes is not freely accessible to these autacoids. The implications of the finding that some cell membranes are impermeable to prostaglandins are discussed.

Colocalization of GAPDH and band 3 (AE1) proteins in rat erythrocytes and kidney intercalated cell membranes

1992 ◽  
Vol 262 (5) ◽  
pp. F892-F896 ◽  
Author(s):  
L. Ercolani ◽  
D. Brown ◽  
A. Stuart-Tilley ◽  
S. L. Alper
Keyword(s):  
Plasma Membrane ◽  
Cell Membranes ◽  
Basolateral Membrane ◽  
Integral Membrane Protein ◽  
Band 3 ◽  
Erythrocyte Membranes ◽  
Rat Erythrocytes ◽  
Kidney Medulla ◽  
Multifunctional Protein ◽  
Alternatively Spliced

Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.2.12) (GAPDH) is a multifunctional protein that associates with the cytoplasmic face of intact human erythrocyte membranes. This association has been postulated to be critically dependent on the interaction of GAPDH with the highly acidic NH2-terminal domain of the principal integral membrane protein of the erythrocyte plasma membrane, the band 3 anion exchanger (AE1). This domain is not conserved in murine erythrocyte AE1 and is fully deleted in the alternatively spliced AE1 isoform that is expressed in the kidney. The lack of conservation of this domain has been proposed to explain the reported absence of GAPDH association with rodent erythrocyte membranes. To determine whether GAPDH could be associated with AE1 proteins in rodent cell membranes, specific rabbit antibodies to peptide sequences of rat GAPDH and mouse AE1 were used to immunolocalize these proteins in sequential semithin sections of rat erythrocytes and kidney medulla. In rat erythrocytes, GAPDH immunoreactivity was predominantly membrane associated and colocalized with AE1. In the kidney medulla, GAPDH was concentrated in the basolateral membrane of type A intercalated cells, where it colocalized with the alternatively spliced kidney form of AE1. GAPDH immunoreactivity was not detected in the plasma membrane of any other cell type in the kidney, indicating its predominant association with AE1-rich membranes. If this membrane interaction occurs via AE1 binding, then GAPDH must have binding sites in addition to those previously described for such binding in human AE1.

scholarly journals Short communications. Subunit A from cholera toxin is an activator of adenylate cyclase in pigeon erythrocytes

1975 ◽  
Vol 146 (1) ◽  
pp. 269-271 ◽  
Author(s):  
S Van Heyningen ◽  
C A King
Keyword(s):  
Adenylate Cyclase ◽  
Cholera Toxin ◽  
Cell Membranes ◽  
Erythrocyte Membranes ◽  
Subunit A ◽  
Subunit B

Intact cholera toxin and its purified subunit A both activate the adenylate cyclase of pigeon erythrocyte membranes, but subunit B does not. The activation by subunit A is unaffected by treatments that inhibit whole toxin by interfering with the binding of subunit B to cell membranes.

scholarly journals THE ACTIVITY OF TRANSMEMBRANE OXYGEN TRANSPORT AND PHYSICAL AND CHEMICAL PROPERTIES OF ERYTHROCYTE MEMBRANES IN PATIENTS WITH DIABETIC FOOT

2016 ◽  
pp. 68-74
Author(s):  
G. A. Stychnevsky ◽  
A. S. Osochuk
Keyword(s):  
Diabetic Foot ◽  
Chemical Properties ◽  
Healthy People ◽  
Erythrocyte Membranes ◽  
Physical And Chemical Properties ◽  
Oxygen Release ◽  
Lipid Pool ◽  
Key Issues ◽  
Physical And Chemical ◽  
And Chemical Properties

The problem of the pathogenesis of diabetic foot being a complication of diabetes mellitus has been very topical up to now. The supply of oxygen to tissues is one of the key issues of the pathogenesis of diabetic foot. The aim of the work was to study the physical and chemical properties of erythrocyte membranes and activity of oxygen transfer in patients with diabetic foot. It has been found that the activity of oxygen release from erythrocytes is the most significant among the studied parameters in detection of patients with diabetic foot and healthy people. Patients with diabetic foot are divided into two groups in terms of physical and chemical properties of erythrocyte membranes and the activity of oxygen release. The first group of patients with diabetic foot is different from healthy people by the activity of oxygen release and micropolarity, microviscosity of annular lipid pool. The second group of patients with diabetic foot differs from healthy people by micropolarity of annular lipid pool (with 2 concentrations of pyrene), microviscosity of total lipid pool and the activity of oxygen release.

scholarly journals Indicators of adrenergic reactivity of erythrocyte membranes in patients with myocardial infarction and nonobstructive atherosclerosis of the coronary arteries after 1 year

2021 ◽  
Vol 10 (Supplement_1) ◽  
Author(s):  
D Vorobyeva ◽  
TYU Rebrova ◽  
SA Afanasyev ◽  
VV Ryabov
Keyword(s):  
Cell Membranes ◽  
Acute Myocarditis ◽  
The Body ◽  
Erythrocyte Membranes ◽  
Control Group ◽  
Index Hospitalization ◽  
Funding Sources ◽  
Artery Disease ◽  
Time Of Admission ◽  
And Control

Abstract Funding Acknowledgements Type of funding sources: None. Background We hypothesized that MINOCA patients have distinctive features of sympatho-adrenal system (SAS) activation in comparison with patients with stenosis atherosclerosis which can play a significant role in the development of ischemic events at the time of the index hospitalization and after 1 year. Aim To study the parameters of β-adrenoreception of cell membranes in patients with MINOCA compared with patients with AMI and single-vessel coronary artery disease after 1 year. Material and methods: The study is non-randomized open controlled. Adrenergic reactivity of the body was assessed by the method for assessing the β-adrenergic reactivity of erythrocyte membranes (β-ARM) for studying the parameters of adrenergic reception of cell membranes. This parameter (β-ARM) was studied upon admission, at days 2, 4 and 7 and 1 year after AMI. The normal level of β- ARM <20 rel.units. Results The study included 40 patients with AMI (19 patients in the main group and 21 patients in the control group). Three patients (15.7%) with diagnosed acute myocarditis were excluded from the analysis. The median age in the main and control groups was 66 (54; 70) years and 59 (55; 65) years, respectively. These groups were different at the admission in such parameters: in smoking frequency (31,3% vs 52.3%), history of angina pectoris (62,5% vs 28,5%), time of admission to the hospital (390 min. vs 180 min.) and thrombolytic therapy at the prehospital phase (3% vs 11%), p < 0,05.  The median β-ARM in MINOCA patients upon admission was 41.7 (29.0; 61.5) rel. units, 1 day - 48.6 (38.5; 57.3) rel. units, 4 days - 49, 4 (39.0; 63.3) rel. units, 7 days - 53.5 (35.2; 67.7) rel. units, after 1 year - 35.7 (25.5; 42.6) rel. units. In the control group, the median β-ARM upon admission was 52.5 (25.4; 64.5) rel. units, 1 day –51.6 (28.3; 56.9) rel. units, 4 days - 48, 5 (34.9; 61.2) rel. units, 7 days - 45.1 (32.2; 68.9) rel. units, after 1 year - 20.8 (14.8; 29.3). In MINOCA patients β-ARM indices in the early postinfarction period statistically higher than the 1 year:  at  1, 2, 4 days, p <0.05, on day 7 no differences were found (p = 0.34). A dynamic comparison of β-ARM indicators in the control group at the time of the index hospitalization and through 1 year revealed differences  at all days early postinfarction period, p <0.05. In the control group, the β-ARM indicator reached normal values by 1-year follow-up period. In MINOCA patients, β-ARM indices after 1 year were statistically higher than in the control group, p = 0.008. Conclusions: The β - ARM indices in MINOCA patients after 1 year from the ischemic event are higher than in the control group. In dynamics, the β - ARM indices statistically decreased in the control group, but did’n change in MINOCA patients. Despite the use of a beta-blocker in MINOCA patients, increased SAS activity persists; therefore, β-APM values did’n change significantly after 1 year.

scholarly journals Genes and Dietary Fatty Acids in Regulation of Fatty Acid Composition of Plasma and Erythrocyte Membranes

Nutrients ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1785 ◽  
Author(s):  
Maria Lankinen ◽  
Matti Uusitupa ◽  
Ursula Schwab
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Cell Membranes ◽  
Dietary Fatty Acids ◽  
Erythrocyte Membranes ◽  
Extensive Literature ◽  
Chromosome 11 ◽  
Fatty Acid Compositions

The fatty acid compositions of plasma lipids and cell membranes of certain tissues are modified by dietary fatty acid composition. Furthermore, many other factors (age, sex, ethnicity, health status, genes, and gene × diet interactions) affect the fatty acid composition of cell membranes or plasma lipid compartments. Therefore, it is of great importance to understand the complexity of mechanisms that may modify fatty acid compositions of plasma or tissues. We carried out an extensive literature survey of gene × diet interaction in the regulation of fatty acid compositions. Most of the related studies have been observational studies, but there are also a few intervention trials that tend to confirm that true interactions exist. Most of the studies deal with the desaturase enzyme cluster (FADS1, FADS2) in chromosome 11 and elongase enzymes. We expect that new genetic variants are being found that are linked with the genetic regulation of plasma or tissue fatty acid composition. This information is of great help to understanding the contribution of dietary fatty acids and their endogenic metabolism to the development of some chronic diseases.

scholarly journals Mechanical properties of sickle cell membranes

Blood ◽  
1990 ◽  
Vol 75 (8) ◽  
pp. 1711-1717 ◽  
Author(s):  
R Messmann ◽  
S Gannon ◽  
S Sarnaik ◽  
RM Johnson
Keyword(s):  
Mechanical Properties ◽  
Blood Cell ◽  
Sickle Cell ◽  
Red Blood Cell ◽  
Cell Membranes ◽  
Erythrocyte Membranes ◽  
Sickle Cells ◽  
Dense Cell ◽  
Cell Fractions ◽  
Membrane Mechanical Properties

Abstract The mechanical properties of sickle erythrocyte membranes were evaluated in the ektacytometer. When ghosts from the total red blood cell population were examined, the rigidity of the resealed ghosts and their rate of fragmentation by shear stress (t1/2) were normal. However, fractionation on Stractan density gradients revealed that sickle cells were heterogenous in their membrane mechanical properties. The ghosts from dense cell fractions exhibited both increased rigidity and decreased stability. Presumably, these altered mechanical properties are a reflection of the well-documented biochemical damage found in irreversibly sickle cell membranes. Nevertheless, neither of the alterations in mechanical properties are likely to be significant elements in the hemolysis of sickle cell anemia. Earlier studies of abnormal erythrocytes suggest that increases in membrane rigidity per se do not increase hemolysis, and they are, therefore, unlikely to do so in this case. The stability of membranes from the dense cell fractions was reduced to about two thirds of the control value. Comparison with the results of studies of red blood cell membranes with genetically defective or deficient spectrin suggests that a reduction in t 1/2 of 50% is not associated with significant increases in the rate of hemolysis. Although altered ghost stability and flexibility can be demonstrated in dense sickle cells, these changes in membrane mechanical properties are not likely to be significant factors in the hemolytic process.

scholarly journals Increased susceptibility of the sickle cell membrane Ca2+ + Mg(2+)- ATPase to t-butylhydroperoxide: protective effects of ascorbate and desferal

Blood ◽  
1992 ◽  
Vol 79 (5) ◽  
pp. 1334-1341
Author(s):  
RB Moore ◽  
TM Hulgan ◽  
JW Green ◽  
LD Jenkins
Keyword(s):  
Sickle Cell ◽  
Cell Membranes ◽  
Sodium Dodecyl ◽  
Thiobarbituric Acid ◽  
Iron Chelator ◽  
Sulfhydryl Groups ◽  
Erythrocyte Membranes ◽  
Protective Effects ◽  
Ferrous Chloride ◽  
Protein Polymerization

Normal and sickle cell erythrocyte membranes were examined for significant differences in their ATPase activities, thiobarbituric acid reactive products formed (measured relative to malondialdehyde), membrane protein polymerization, and number of protein-free sulfhydryl groups when treated with 0.5 mmol/L t-butylhydroperoxide (tBHP) for 30 minutes. Isolated sickle cell membranes treated with tBHP produced significantly greater inhibition in both their basal and calmodulin- stimulated Ca2+ + Mg(2+)-ATPase activities (75% inhibition in both cases) compared with that of control membranes. In addition, there was significantly more malondialdehyde formed from sickle cell membranes compared with control membranes. Oxidation caused greater protein polymerization in sickle cell membranes compared with normal membranes as demonstrated by the formation of high molecular weight polymers separated on sodium dodecyl sulfate polyacrylamide gels. The number of free sulfhydryl groups present in spectrin and actin decreased more in sickle cell membranes as measured by 3H-N-ethyl maleimide autoradiography and gel scanning. To prevent enzyme inhibition, erythrocyte membranes were treated with tBHP in the presence of 1 mmol/L ascorbate, a potential antioxidant, and 1 mmol/L desferal, an iron chelator. Both ascorbate and desferal added alone with tBHP were effective in preventing inhibition of the basal and calmodulin- stimulated Ca2+ + Mg(2+)-ATPase activities in normal membranes, but in sickle cell membranes only the addition of ascorbate and desferal together offered significant protection. The enhanced oxidation observed with sickle cell membranes can be mimicked in normal white membranes by adding hemoglobin, hemin, or ferrous chloride in the presence of tBHP. In contrast to hemoglobin, ferrous chloride has the ability to enhance membrane oxidation in the presence of ascorbate with or without tBHP. Furthermore, the addition of desferal to these membranes greatly decreased the iron-ascorbate-tBHP oxidation of erythrocyte membranes as determined by the sustained ATPase activities and the reduced formation of malondialdehyde. Maximal protection was provided by 1 mmol/L desferal in the presence of 1 mmol/L ascorbate, although some protection was observed even at 10 mumol/L, the lowest concentration tested. These results are discussed in light of the pro- and anti-oxidant effects of ascorbate in the absence and presence of iron and tBHP.

scholarly journals Some peculiarities of personal profile and morphovegetative constituthion in healthy people at different level of na+/li+ countertransport in erythrocyte membranes

1998 ◽  
Vol XXX (1-2) ◽  
pp. 20-25
Author(s):  
D. R. Khasanova ◽  
М. F. Ismagilov ◽  
V. D. Mendelevich ◽  
V. N. Oslopov ◽  
Е. А. Baranova
Keyword(s):  
Genetic Marker ◽  
Personal Characteristics ◽  
Anxiety Level ◽  
Healthy People ◽  
Erythrocyte Membranes ◽  
Velocity Range ◽  
Personal Profile

Personal characteristics in combination of vegetative homeostasis were studied, and also type of morphoconstitution in association with one of genetic marker - velocity of Na+/ Li+ countertransport in erythrocyte membranes of 192 healthy people, aged from 17 to 42 y.o. Trends to increase the anxiety level in patients of both sex in all age groupes were determined at the velocity range of Na + /Li+ countertransport in erythrocytes: from 162 to 278 mс Mol Li/1 c h, which were most expressed in asthenic morphoconstitution people with sympathicotonic type of vegetative effects, this being combined with disadaptive forms of vegetative reactions.

Erythrocyte phosphoinositide metabolism in essential hypertensive patients and their normotensive offspring

1987 ◽  
Vol 73 (1) ◽  
pp. 29-32 ◽  
Author(s):  
A. Riozzi ◽  
A. M. Heagerty ◽  
J. D. Ollerenshaw ◽  
J. D. Swales
Keyword(s):  
Blood Pressure ◽  
At Risk ◽  
Essential Hypertension ◽  
Erythrocyte Membranes ◽  
Phosphoinositide Metabolism ◽  
Hypertensive Patients ◽  
Metabolic Turnover ◽  
32P Incorporation ◽  
Phosphatidylinositol 4 ◽  
Genetically Determined

1. The metabolism of phosphoinositides was investigated in erythrocyte membranes of essential hypertensive patients, normotensive offspring of hypertensive patients and matched controls. 2. Measurement of 32P-labelling of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bis-phosphate revealed no differences in the rate of incorporation of the isotope in essential hypertensive patients compared with controls. 3. In the normotensive offspring of essential hypertensive patients there was a highly significant increase in the rate of 32P incorporation (P < 0.01), compared with matched controls, indicating a higher rate of metabolic turnover in these subjects. 4. These data demonstrate that phosphoinositide metabolism is enhanced in subjects genetically at risk of hypertension, before the blood pressure has risen, but once the blood pressure is established, it is no different from control values. Abnormal phosphoinositide metabolism may be a further manifestation of a genetically determined defect of membrane physicochemical function underlying essential hypertension.

Sign in / Sign up

Export Citation Format

Share Document