scholarly journals Analysis of concentration and activity of proteins involved in iron metabolism in rats with streptozotocin-induced hyperglycemia

2020 ◽  
Author(s):  
Irina V. Voynova ◽  
Valeria A. Kostevich ◽  
Anna Yu. Elizarova ◽  
Marina N. Karpenko ◽  
Alexey V. Sokolov
Keyword(s):  
Iron Metabolism ◽  
Binding Capacity ◽  
Iron Concentration ◽  
Total Iron ◽  
Saline Control ◽  
Serum Concentrations ◽  
Compensatory Response ◽  
Ferroxidase Activity ◽  
Iron Binding Capacity ◽  
Homeostatic Response

Objective. We aimed to analyze the alterations of concentration and activity of iron metabolism proteins in samples obtained from rats with hyperglycemia induced by streptozotocin (STZ). Methods. Concentration and activity of ceruloplasmin (Cp) and transferrin (Tf), concentration of glucose, fructosamine, hemoglobin, ferritin (Ft), and iron were measured in blood samples obtained from rats after injection of STZ or saline (control). To develop in-house ELISA, highly purified preparations of Cp, Tf, and Ft, as well as specific antibodies against these proteins, were obtained. Results. The hyperglycemia in rats after STZ injection was confirmed by increase in glucose and fructosamine serum concentrations. Increase in Cp concentration and decrease in specific ferroxidase activity of Cp, concentration of Tf and Ft were observed in hyperglycemic rats. However, the absence of changes in iron concentration and total iron binding capacity of serum is indicative of compensatory response. Conclusion. STZ-induced hyperglycemia in rats was characterized by alteration of activity and concentration of iron metabolism proteins, however, this alteration was negated by homeostatic response. The alterations of Cp and Tf concentrations observed in this study are similar to those in acute phase of inflammation.

scholarly journals Dynamics of changes in iron concentration and total iron binding capacity in blood plasma of goat kids during their first month of life (Short Communication)

2009 ◽  
Vol 52 (4) ◽  
pp. 419-424
Author(s):  
W. F. Skrzypczak ◽  
M. Ożgo ◽  
A. Lepczyński ◽  
A. Łata
Keyword(s):  
Blood Plasma ◽  
Binding Capacity ◽  
Iron Concentration ◽  
Total Iron ◽  
Postnatal Life ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Plasma Iron ◽  
Iron Binding Capacity ◽  
In Blood Plasma

Abstract. The experiment was carried out on 14 kids of Polish Improved White breed during the first 30 days of life. The aim of this study was to show changes in the concentration of blood plasma iron and total iron binding capacity (TIBC) during the neonatal period. The statistically confirmed differences (P≥0.01) in blood serum iron concentration was show in the kids between 5th (20.57 μmol/l) and 14th (9.97 μmol/l) day of life, and between 3rd and 4th week with the peak in 23rd day (27.50 μmol/l). We have also shown statisticly confirmed increase in TIBC (P≥0.01) between 1st and 4th day of life (+20.13 μmol/l), and between 14th and 23rd day of life (+15.59 μmol/l). Statistically confirmed decrease of TIBC was observed between 6th (52.30 μmol/l) and 14th (31.78 μmol/l) day of life. Conclusion: The studies have revealed dynamic changes in the concentration and total binding capacity of blood plasma iron concentration during the first month of postnatal life. The pattern of such changes does not depend on the gender or litter size. It was observed a significant decrease in the concentration of this trace element in blood plasma toward the end of the second week, which may indicate a relative iron deficiency, particularly in twin-born kids.

scholarly journals Effects of Transferrin (TF) Polymorphism on the Levels of Some Blood Serum Indices in Clinically Healthy Piglets and Piglets with Diarrhoea Caused by <i>Escherichia coli</i>

2006 ◽  
Vol 49 (5) ◽  
pp. 484-493
Author(s):  
G. M. Życzko ◽  
K. Życzko ◽  
J. Osek
Keyword(s):  
Blood Serum ◽  
Binding Capacity ◽  
Iron Concentration ◽  
Total Iron ◽  
Total Iron Binding Capacity ◽  
E Coli ◽  
Iron Saturation ◽  
Suckling Piglets ◽  
Iron Binding Capacity ◽  
Made In

Abstract. The effect of transferrin (TF) polymorphism and the levels of some blood serum indices, functionally linked to this protein, were studied. The following determinations were made in the blood serum of 311 hybrid suckling piglets - TF polymorphism, TIBC (total iron binding capacity), TF iron saturation percentage and levels of iron ceruloplasmin (CP) and copper. Clinically healthy piglets and piglets with diarrhoea caused by E. coli were divided into younger (aged 10 to 14 days) and older (aged 15 to 28 days). The piglets showed two genotypes, TF AB and TF BB. The TF BB genotype dominated among clinically healthy piglets, and the TF AB genotype in those suffering from diarrhoea. TF polymorphism differentiated the levels of CP and copper in clinically healthy piglets, and modified iron concentration and TF iron saturation percentage in piglets with diarrhoea. Regardless of health status, age had an almost identical effect on the levels of blood serum indices in piglets of both genotypes. The differences in the levels of CP, iron, and TF iron saturation percentage in healthy piglets and piglets with diarrhoea were modified by the presence of the TFA allele, but in older piglets only.

scholarly journals Iron-induced ascorbate oxidation in plasma as monitored by ascorbate free radical formation. No spin-trapping evidence for the hydroxyl radical in iron-overloaded plasma

1992 ◽  
Vol 282 (2) ◽  
pp. 459-465 ◽  
Author(s):  
M Minetti ◽  
T Forte ◽  
M Soriani ◽  
V Quaresima ◽  
A Menditto ◽  
...  
Keyword(s):  
Free Radical ◽  
Binding Capacity ◽  
Iron Concentration ◽  
Spin Trapping ◽  
Iron Loading ◽  
Ascorbate Free Radical ◽  
Plasma Ascorbate ◽  
Ferroxidase Activity ◽  
Iron Binding Capacity

A study was made of the interaction of plasma ascorbate and ascorbate free radical (AFR) with exogenously added iron. The quantitative determination of AFR has the advantage that transient increases in ascorbate oxidation can be directly monitored by e.p.r. spectroscopy. An AFR signal was found in the plasma of all donors and was unaffected by superoxide dismutase, catalase and the strong iron chelator deferoxamine. These findings and the rapid decrease in AFR under a nitrogen atmosphere suggest that plasma AFR is probably a result of air auto-oxidation. Iron loading of plasma did not affect the intensity of the AFR signal until the iron concentration approached or exceeded the plasma latent iron-binding capacity. In iron-overloaded plasma, the intensity of the AFR signal increased to about 10 times the normal level before decreasing rapidly to undetectable levels after 15-20 min. Determination of plasma ascorbate showed that the disappearance of AFR was due to a complete loss of the vitamin. When 50 microM-ascorbate was loaded with iron in iso-osmotic phosphate buffer there was an increase in the AFR signal, independent of the iron concentration, which was stable at least for 15 min. Thus the rate of ascorbate loss in the iso-osmotic phosphate buffer was considerably lower than in iron-overloaded plasma. The addition of different iron chelators produced comparable effects on the intensity of the AFR signal in both iron-overloaded plasma and ascorbate solution. These results suggest that the characteristic behaviour of plasma AFR after iron loading is due to its specific iron-binding capacity and to plasma ferroxidase activity. The ferroxidase activity of plasma is important to promote the transfer of Fe2+ into transferrin without a transient ascorbate oxidation. Spin-trapping studies with 5,5-dimethyl-1-pyrroline N-oxide and N-t-butyl-alpha-phenylnitrone revealed that iron-overloaded plasma was unable to produce spin-trap adducts even in the presence of 50-300 microM-hydrogen peroxide or 100 microM-azide. Evidence of OH. radical formation was obtained only after the addition of EDTA. Therefore, iron-overloaded plasma itself does not produce a Fenton reaction and, if ascorbate does indeed have a free-radical-mediated pro-oxidant role, it is not detectable in plasma by spin-trapping experiments.

Changes of iron metabolism during pregnancy and the establishment of reference intervals for pregnant Chinese women

2019 ◽  
Vol 56 (5) ◽  
pp. 556-563
Author(s):  
Yuanqing Yang ◽  
Rong Wang ◽  
Hongmin Jiang ◽  
Min Hu ◽  
Aiguo Tang ◽  
...  
Keyword(s):  
Serum Ferritin ◽  
Iron Metabolism ◽  
Serum Iron ◽  
Binding Capacity ◽  
Transferrin Saturation ◽  
Reference Intervals ◽  
Total Iron ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Iron Binding Capacity

Background Abnormalities of iron metabolism in pregnancy pose risks for maternal and fetal health. Robust reference intervals for iron metabolism indices have not been established in a pregnant Chinese population. The purpose of this study was to derive reference intervals for indices of iron metabolism during pregnancy in a Chinese population. Methods A total of 360 healthy pregnant women were recruited and divided into three groups of 120 by gestational age: first trimester (1–13 weeks), second trimester (14–27 weeks) and third trimester (≥28 weeks). An additional 120 healthy non-pregnant women were recruited as the non-pregnant control group. Serum ferritin was measured by electrochemiluminescence immunoassay. Serum iron and total iron-binding capacity were measured by a direct bathophenanthroline method. Transferrin saturation value was calculated with formula TS = SI/TIBC. The reference intervals were established using a non-parametric method. Results In first and second trimesters (combined), the reference intervals for serum ferritin, serum iron, total iron-binding capacity and transferrin saturation are 14.7–184.3 mg/L, 14.50–33.45 µmol/L, 36.53–68.81 µmol/L and 19.04–64.76%, respectively. In the third trimester, the reference intervals for serum ferritin, serum iron, total iron-binding capacity and transferrin saturation are 7.2–122.2 mg/L, 5.83–21.52 µmol/L, 49.40–122.76 µmol/L and 8.22–52.75%, respectively. Conclusion The reference intervals for iron metabolism indices for healthy pregnant Chinese women were established in accordance with CLSI C28-A3 guidelines. This will be a valuable tool for clinical practice and research.

scholarly journals Iron Metabolism in Field Hockey Players During an Annual Training Cycle

2015 ◽  
Vol 47 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Tomasz Podgórski ◽  
Jakub Kryściak ◽  
Jan Konarski ◽  
Katarzyna Domaszewska ◽  
Krzysztof Durkalec-Michalski ◽  
...  
Keyword(s):  
Oxygen Uptake ◽  
Iron Metabolism ◽  
Human Body ◽  
Binding Capacity ◽  
Iron Concentration ◽  
Iron Binding ◽  
Field Hockey ◽  
Training Cycle ◽  
Hockey Players ◽  
Iron Binding Capacity

AbstractPost-physical training changes in iron metabolism in the human body often occur. To fully describe these processes, fifteen male Polish National Team field hockey players (age 27.7 ± 5.2 years, body mass 72.8 ± 7.6 kg and body height 177.1 ± 5.7 cm) were examined in three phases of an annual training cycle: preparatory (T1), competitive (T2) and transition (T3). To assess aerobic fitness, maximal oxygen uptake (VO2max) was evaluated. Based on the iron concentration, the changes in total iron binding capacity (TIBC), unsaturated iron binding capacity (UIBC) and other selected haematological indicators (haemoglobin, erythrocytes, mean corpuscular haemoglobin - MCH) in iron metabolism were estimated. The average values of maximum oxygen uptake increased from 54.97 ± 3.62 ml·kg−1·min−1in T1 to 59.93 ± 3.55 ml·kg−1·min−1in T2 (p<0.05) and then decreased to 56.21 ± 4.56 ml·kg−1·min−1in T3 (p<0.05). No statistically significant changes in the erythrocyte count were noted. The MCH and haemoglobin concentration decreased between T1 and T2. The maximal exercise test caused a significant (p<0.05) increase in the plasma iron concentration during the competition and transition phases. Progressive but non-significant increases in resting iron concentration, TIBC and UIBC in the analysed annual training cycle were noted. To show global changes in iron metabolism in the human body, it is necessary to determine additional variables, i.e. UIBC, TIBC, haemoglobin, MCH or the erythrocyte count. The direction of changes in iron metabolism depends on both the duration and intensity of the physical activity and the fitness level of the subjects. Dietary intake of iron increases the level of this trace element and prevents anaemia associated with training overloads.

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