scholarly journals The characteristics of serum circulating immune complexes of patients with atopic asthma with different severity degree

2013 ◽  
Vol 94 (5) ◽  
pp. 744-748 ◽  
Author(s):  
Y V Skibo ◽  
N S Kurmaeva ◽  
V N Tsibulkina ◽  
I G Mustafin ◽  
Z I Abramova
Keyword(s):  
Severe Asthma ◽  
Bronchial Asthma ◽  
Immune Complexes ◽  
Wave Length ◽  
Molecular Complexes ◽  
Electrophoretic Analysis ◽  
Circulating Immune Complexes ◽  
Atopic Asthma ◽  
Control Group ◽  
Serum Samples

Aim. To evaluate the serum level of pathogenic circulating immune complexes in patients with mild and severe atopic bronchial asthma. Methods. Serum samples of patients with atopic asthma of mild persistent (30 patients) and severe persistent (20 patients) forms were analyzed. The control group consisted of 15 healthy volunteers. To detect the giant, large, medium and small-sized serum immune complexes, 3, 3.5, 4 and 7% polyethyleneglycol-6000 solutions were used. For quantitative evaluation of the immune complexes we measured the ultraviolet optical density at 280 nm wave length. To separate the immune complexes from immunoglobulin, Protein-G-Sepharose was used. Determination of the protein composition of circulating immune complexes was performed by electrophoresis in 8% polyacrylamide gel. Results. The concentration of immune complexes was increased in patients with bronchial asthma compared to healthy donors. Small and medium-sized immune complexes were prevailing, their concentrations correlated with the severity of asthma. Large, medium and small-sized immune complexes participated in immunopathological reactions in patients with both mild and severe asthma, with immune complexes pathogenicity coefficient significantly increased depending on the severity of the disease. Electrophoretic analysis of circulating immune complexes has shown the presence of proteins with molecular weight of 60 kDa in the complexes of all sizes. In the severe asthma group, an antigen fraction with a molecular mass of 36 kDa within the small-sized molecular complexes was revealed. Conclusion. The observed increase of small and medium-sized circulating immune complexes serum levels in patients with bronchial asthma may be an indicator of of these patients predisposal to autoimmune reactions development.

scholarly journals Role of Circulating Immune Complexes in the Pathogenesis of Canine Leishmaniasis: New Players in Vaccine Development

2021 ◽  
Vol 9 (4) ◽  
pp. 712
Author(s):  
Cristina Cacheiro-Llaguno ◽  
Nuria Parody ◽  
Marta R. Escutia ◽  
Jerónimo Carnés
Keyword(s):  
Immune Complexes ◽  
Vaccine Development ◽  
Correct Diagnosis ◽  
Circulating Immune Complexes ◽  
Response To Treatment ◽  
Canine Leishmaniasis ◽  
Leishmania Infection ◽  
Serum Samples ◽  
Humoral Immune

During canine visceral leishmaniasis (CanL), due to Leishmania infantum (L. infantum), uncontrolled infection leads to a strong humoral immune response. As a consequence of the production of high antibody levels and the prolonged presence of parasite antigens, circulating immune complexes (CIC) are formed, which can be deposited in certain organs and tissues, inducing vasculitis, uveitis, dermatitis and especially glomerulonephritis and renal failure. A method to detect CIC and quantify their levels in serum samples from dogs infected with L. infantum has been recently described. It allowed demonstration of a correlation between CIC levels and disease severity. Thus, CIC measurement may be useful for diagnosis, assessment of disease progression and monitoring response to treatment. This is an interesting finding, considering that there remains an urgent need for identification of novel biomarkers to achieve a correct diagnosis and for optimal disease staging of dogs suffering from Leishmania infection. The objective of the present review is to shed light on the role of CIC in CanL, as well as to highlight their potential use not only as diagnostic and prognostic biomarkers but also as a valuable tool in vaccine development and new immunotherapy strategies to prevent or control disease outcome.

scholarly journals Effects of the Airway Obstruction on the Skin Microcirculation in Patients with Bronchial Asthma

2016 ◽  
Vol 71 (3) ◽  
Author(s):  
Irina V. Tikhonova ◽  
N. I. Kosyakova ◽  
A. V. Tankanag ◽  
N. K. Chemeris
Keyword(s):  
Blood Flow ◽  
Airway Obstruction ◽  
Bronchial Asthma ◽  
Skin Blood Flow ◽  
Peripheral Blood ◽  
Atopic Asthma ◽  
Control Group ◽  
Bronchial Obstruction ◽  
Peripheral Blood Flow ◽  
Flow Parameters

Background: Pulmonary hemodynamic disorders depend on the inflammatory phases and severity of the obstructive syndrome. However, the effect of asthma bronchial obstruction on the state of peripheral hemodynamics remains insufficiently known. Aims: To study the effects of airway obstruction on skin blood flow parameters and its regulatory systems in patients with persistent atopic bronchial asthma in the remission state.Materials and methods: A comparative study of the skin peripheral blood flow in patients with bronchial asthma with severe airway obstruction (1st group) and without obstruction (2nd group) was conducted. 20 patients with confirmed diagnosis of atopic asthma of 50–74 years old participated in the study. All patients received basic therapy in a constant dosing of high doses of inhaled glucocorticosteroids/long-acting beta-2-agonists. The control group included 20 healthy volunteers without evidence of bronchial obstruction. The study lasted for 3 months. The forced expiratory volume in 1 s (FEV1) was used to evaluate the bronchial obstruction by spirometry technique. Skin blood perfusion changes were recorded by laser Doppler flowmetry at rest and in response to short-term local ischemia. Registered peripheral blood flow signals were examined using the amplitude temporal filtering in five frequency intervals to identify the functional features of the peripheral blood flow regulation systems. Results: Consistent two-fold decrease of the oscillation amplitudes was found in the neurogenic interval at rest (p=0.031), as well as in the myogenic (p=0.043; p=0.031) and endothelial intervals (p=0.037; p≤0.001) both at rest and during the postocclusive reactive hyperemia respectively in the 1st group of patients with bronchial obstruction (FEV1 80%) compared with the control group. No significant changes were revealed for skin blood flow parameters in the 2nd patient group (without obstruction, FEV1 80%) in comparison to control subjects.Conclusions: The presence of bronchial obstruction has a significant impact on the changes of the amplitudes of skin blood flow oscillations in patients with bronchial asthma in the myogenic, neurogenic and endothelial intervals.

Detection of drug specific circulating immune complexes from in vivo cynomolgus monkey serum samples

2015 ◽  
Vol 416 ◽  
pp. 124-136 ◽  
Author(s):  
Piotr Pierog ◽  
Murli Krishna ◽  
Aaron Yamniuk ◽  
Anil Chauhan ◽  
Binodh DeSilva
Keyword(s):  
Immune Complexes ◽  
Cynomolgus Monkey ◽  
Circulating Immune Complexes ◽  
Serum Samples

scholarly journals FEATURES OF IMMUNOLOGICAL REACTIVITY OF PATIENTS WITH LICHEN PLANUS OF THE ORAL MUCOSA

2021 ◽  
Vol 17 (3) ◽  
pp. 24-28
Author(s):  
Vladimir Bikmurzin ◽  
Ludmila Balyabina ◽  
Irina Gimraniva ◽  
Guzel' Akmalova ◽  
Sergey Chuykin ◽  
...  
Keyword(s):  
Lichen Planus ◽  
Oral Mucosa ◽  
Immune Complexes ◽  
Clinical Laboratory ◽  
Dental Materials ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Immune Reactivity ◽  
Immunological Reactivity ◽  
Clinical Forms

Lichen planus is considered as a multifactorial disease. Various factors play a role in the development of lichen planus of the oral mucosa: genetic, dental materials that are used for restoration, prosthetics of teeth, Helicobacter pylori, viruses, psychological stress, somatic pathology and others. The pathogenesis of oral lichen planus is based on an autoimmune process. The aim of the study was to determine the immunological reactivity in patients with various clinical forms of lichen planus of the oral mucosa. The study carried out clinical, laboratory examination of 75 female patients aged 29 to 75 years old with red lichen planus of the oral mucosa. All patients were divided into three groups depending on the clinical form of the disease. The control group consisted of 30 women aged 39 to 70 years old without red lichen planus of the oral mucosa. The phagocytic activity of neutrophils, the level of immunoglobulins of classes A, M, G and circulating immune complexes in the blood serum were evaluated. The humoral link of immunity in patients with exudative-hyperemic form of OLP was characterized by hyperimmunoglobulinemia of classes A, M, G, an increase in circulating immune complexes, in patients with erosive-ulcerative form of hyperimmunoglobulinemia of class M against the background of hypoimmunoglobulinemia A, G and an increase in circulating immune complexes. In patients with a typical form of OLP, only an increase in the level of circulating immune complexes was noted. When assessing the immune reactivity in patients with OLP, the relationship between the manifestations of the "dysphagocytosis" syndrome, disorders in the humoral immunity system with various clinical forms of the disease was established. This fact allows us to conclude about the pathogenetic role of an imbalance in the system of mechanisms that ensure the elimination of pathogens, including infectious nature in the occurrence of a disease with the formation of an autoimmune component.

scholarly journals Arterial dysfunction and systemic inflammation in patients with bronchial asthma

2012 ◽  
Vol 9 (1) ◽  
pp. 42-49
Author(s):  
E A Sobko ◽  
A Y Kraposhina ◽  
O P Ischenko ◽  
I V Demko ◽  
A B Salmina ◽  
...  
Keyword(s):  
Asthma Control ◽  
Severe Asthma ◽  
Bronchial Asthma ◽  
Peripheral Blood ◽  
Vascular Wall ◽  
Control Group ◽  
External Respiration ◽  
Steroid Dependent ◽  
Healthy Donors ◽  
And Function

Background. The objective of this study was to estimate a vascular wall status of large arteries and function of endothelium in patients with different clinical forms of bronchial asthma throughout the disease progression. 220 patients with bronchial asthma have been examined, including 106 persons with moderate asthma ( 1 st group), 61 persons with severe asthma (2 nd group), and 53 persons with steroid-dependent asthma. Control group was formed from 40 healthy donors. Methods. We have assessed parameters of external respiration, arterial rigidity, and the levels of TNFα, IL-6, sCD31 (sPECAM-1), CRP in the peripheral blood at the time of exacerbation and 48 weeks later. Results. We found elevation of IL-6 and TNFα levels in all the tested groups in the period of exacerbation comparing to the control group (p

scholarly journals ОСОБЛИВОСТІ ІМУННОГО ГОМЕОСТАЗУ В ДИНАМІЦІ РОЗВИТКУ ЕКСПЕРИМЕНТАЛЬНОЇ БРОНХІАЛЬНОЇ АСТМИ

2018 ◽  
Author(s):  
M. S. Reheda ◽  
L. A. Lubinets ◽  
B. F. Shchepanskyi
Keyword(s):  
Immune System ◽  
B Lymphocytes ◽  
Immune Complexes ◽  
Guinea Pigs ◽  
Circulating Immune Complexes ◽  
System Response ◽  
Control Group ◽  
Modeling Process ◽  
Immune System Response

In this paper, it is shown that the modeling process of bronchial asthma (BA ) is accompanied by changes, in comparison with the control group, in the indicators of immune system response: T- & B-lymphocytes, circulating immune complexes in blood of guinea pig males on the 4th, 18th, 25th day of experiment.The aim of the study – determination of some indices of immune system in blood of guinea pigs in the modeling process of BA on 4th, 18th, 25th day of the experiment.Materials and Methods. Experiments were conducted on 40 guinea pigs (males), with body weight 0.25–0.27 kg. Animals were divided into four groups of ten animals in each. Intact guinea pigs were the first group. Animals with experimental BA – the second, third, fourth group respectively on the 4th, 18th, 25th day of the experiment. Experimental BA was reproduced by V. I. Babych method. In blood of intact guinea pigs and animals with experimental BA , the number of T- and B-lymphocytes was determined by the method of E. F. Chernushenko, L. S. Kohosov, determination of the level of circulating immune complexes was carried out by the method of V. Haskova and co-authors. The results of the study were processed by the method of variation statistics using Student's criterion.Results and Discussion. The results of the studies showed unidirectional changes in individual parameters of the immune system, depending on the periods of the formation of BA : an increase in the number of B-lymphocytes and circulating immune complexes, a decrease in the number of T-lymphocytes for all of the studied days of the experiment.Conclusions. The obtained results indicate significant changes in the immune system parameters in the blood of experimental animals with BA and are important for understanding the pathogenesis of BA . These studies provide an opportunity to find the more perfect and effective methods of diagnosis of BA.

scholarly journals The effect of interferons-α and –γ and their combination with dimethyl dipyrazolyl selenide on the immune status and functional state of the reproductive system of cows in adverse ecological conditions

2019 ◽  
pp. 53-57
Author(s):  
A. G. Shakhov ◽  
I. T. Shaposhnikov ◽  
Yu. N. Brigadirov ◽  
V. N. Kotsarev ◽  
V. N. Skorikov ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Functional State ◽  
Reproductive System ◽  
Immune Complexes ◽  
Immune Status ◽  
Lysozyme Activity ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Ecological Conditions ◽  
Positive Effect

The results of studying the effect of interferons alpha and gamma and their combination with dimethyl dipyrazolyl selenide on the immune status and functional state of the reproductive system of cows in adverse ecological conditions are presented. The positive effect of the drugs, especially their combination, on erythropoiesis and the immune status of animals, which was manifested by an increase in the content of erythrocytes, hemoglobin, hematocrit, leukocytes, total immunoglobulins, serum bactericidal and lysozyme activity, absorptive function of phagocytes, relative amount of T lymphocytes, and a decrease in the level of circulating immune complexes was stated. An increase in the immune status of animals at the effect of immunocorrecting drugs was accompanied by a decrease in the pathology of calving and postpartum complications and favorably affected the functional state of their reproductive system. The cows which were introduced interferons and their combination with dimethyl dipyrazolyl selenide demonstrated a shorter period from calving to successful insemination, as well as the insemination index and fertility, compared with the animals of the control group, which were not introduced any drugs.

scholarly journals Clinical and immunological features of opisthorchiasis

2021 ◽  
Vol 102 (5) ◽  
pp. 626-635
Author(s):  
A U Sabitov ◽  
D A Soldatov ◽  
Yu B Khamanova
Keyword(s):  
Immune System ◽  
Immune Complexes ◽  
Statistical Significance ◽  
Rank Correlation ◽  
Statistical Processing ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Cytotoxic Lymphocytes ◽  
Spearman’S Rank Correlation ◽  
Diagnostic Center

Aim. To assess the clinical and immunological features in patients with chronic opisthorchiasis, depending on the duration of the infection. Methods. The first group consisted of 19 patients with the duration of the infection up to 1 year, the second group consisted of 21 patients with the duration of the infection between 1 and 5 years, the third group was formed of 23 patients with the duration of the disease more than 5 years, the control group 20 healthy individuals. Immunological research was carried out at the Clinical Diagnostic Center. Statistical processing was performed using Microsoft Excel 2010 and Statistica 6.0 software. The statistical significance of differences was determined by using the MannWhitney test (U-test) at the level of significance of p 0.05. The correlations were assessed by calculating Spearman's rank correlation coefficients.Results Clinical features of chronic opisthorchiasis were revealed in the disease duration groups of up to 1 year, from 1 year to 5 years, more than 5 years: the subclinical course was most common in the group of up to 1 year; cholangiohepatitis prevailed in the group of between 1 to 5 years, allergic skin syndrome, cholangiocholecystitis and pancreatitis dominated in the group of more than 5 years. The immune response in chronic opisthorchiasis was characterized by: up to 1 year lymphocytosis, increased levels of immunoglobulins M (IgM) and circulating immune complexes (CIC), a decrease in the number of T-lymphocytes (CD3+), as well as an increase in bactericidal activity of leukocytes (BAL); between 1 and 5 years monocytosis, increased levels of immunoglobulins M, immunoglobulins G and circulating immune complexes, a decrease in T-cytotoxic lymphocytes (CD8+) and nitro blue tetrazolium (NBT test), as well as an increase in NK cells and phagocytic activity of monocytes, more than 5 years eosinophilia. Conclusion. Common features of rearrangement of the immune system in opisthorchiasis: inflammatory changes in the hemogram, activation of humoral immunity with parallel suppression of the cellular component of the immune system, and increased phagocytosis.

Circulating Immune Complexes in Human Malignant Melanoma

1982 ◽  
Vol 68 (6) ◽  
pp. 469-472 ◽  
Author(s):  
Raffaele D'Amelio ◽  
Brian Cooke ◽  
John R. Hobbs
Keyword(s):  
Malignant Melanoma ◽  
Immune Complexes ◽  
Solid Phase ◽  
Serum Antibody ◽  
Specific Protein ◽  
Circulating Immune Complexes ◽  
Serum Samples ◽  
Human Malignant Melanoma ◽  
Third Stage ◽  
Solid Phase Assay

The sera from 34 patients with malignant melanoma at various clinical stages of the disease were examined for the presence of circulating immune complexes (CIC) by the C1q solid-phase assay. Their urine and serum samples had been previously examined for the presence of an urinary melanoma-specific protein (MSP) and the corresponding serum antibody. Low levels of CIC (only in the third stage of the disease) and no positive correlation with the presence of MSP were found. The discordance between our and other author's data stresses again the fact that the different laboratory methods for CIC evaluation reveal in a different way the various CIC populations occurring in several diseases.

РАСПРОСТРАНЕННОСТЬ ФЕНОТИПОВ ТЯЖЕЛОЙ БРОНХИАЛЬНОЙ АСТМЫ НА СРЕДНЕМ УРАЛЕ

2019 ◽  
Author(s):  
E.K. Beltyukov ◽  
V.V. Naumova ◽  
V.Ch. Abdullaev ◽  
Y.A. Styazhkina ◽  
S.S. Vedenskaya
Keyword(s):  
Targeted Therapy ◽  
Severe Asthma ◽  
Bronchial Asthma ◽  
Cost Effective ◽  
Atopic Asthma ◽  
Practical Significance ◽  
Asthma Prevalence ◽  
Uncontrolled Asthma ◽  
Asthma Phenotype ◽  
Number Of Patients

Обоснование. Тяжелая БА является гетерогенным и экономически затратным заболеванием, что требует персонифицированного подхода к лечению с включением таргетной терапии, предполагающей фенотипирование астмы. Цель. Определение динамики распространенности бронхиальной астмы (БА) на Среднем Урале, в том числе тяжелой БА, и проведение фенотипирования пациентов с тяжелой БА для определения потребности в таргетной терапии. Материалы и методы. Популяционные исследования распространенности БА проводились на Среднем Урале с 2000 по 2012 г. с использованием стандартного вопросника ECRHS создавались регистры больных БА. В 2018 г. в г. Екатеринбурге проведен анализ амбулаторных карт пациентов с БА (n216). Фенотипирование БА проводилось врачом аллергологом-иммунологом. Результаты. На Среднем Урале за 17 лет число больных БА увеличилось в 2,7 раза. В популяции превалируют больные с легким течением астмы в 70,8-81 случаев преобладает атопический фенотип вне зависимости от категории населения. В структуре зарегистрированных по обращаемости больных астмой тяжелая неконтролируемая БА составляет 10,2. Каждый второй пациент с тяжелой неконтролируемой астмой имеет атопический фенотип, что составляет 5 от числа всех случаев БА каждый четвертый больной из данной группы имеет эозинофильной фенотип, что составляет 2,3 от числа всех случаев (n216). Заключение. Фенотипирование БА имеет важное прикладное значение для планирования эффективной таргетной терапии в популяции больных тяжелой неконтролируемой астмой.Topicality. Severe asthma is a heterogeneous and cost-effective disease that requires a personalized treatment approach with inclusion of targeted therapy involving the phenotyping of asthma. Objective. Determine the dynamics of asthma prevalence in the Middle Ural, including severe asthma, and phenotype patients with severe asthma for the selection of targeted therapy. Materials and methods. Population studies of bronchial asthma prevalence were conducted in the Middle Ural from 2000 to 2012 using the standard ECRHS questionnaire. Also registers of patients with asthma were created. An analysis of outpatient records of patients with asthma was conducted in Ekaterinburg in 2018. The phenotyping of bronchial asthma was carried out by an allergist-immunologist. Results. The number of patients with bronchial asthma increased by 2.7 times over 17 years in the Middle Ural. Patients with mild asthma prevail in the population. The atopic asthma phenotype predominates in 70.8-81 of cases regardless of the population category. Severe uncontrolled bronchial asthma occurs in 10.2 of cases among all patients seeking medical care. Every second patient with severe uncontrolled asthma has an atopic phenotype, which is 5 of the total number of analyzed patients with bronchial asthma. Every fourth patient with severe uncontrolled bronchial asthma has an eosinophilic phenotype, which is 2.3 of all analyzed patients with bronchial asthma (n216). Conclusion. Phenotyping of asthma has important practical significance for planning effective targeted therapy in a population of patients with severe uncontrolled asthma.

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