scholarly journals Influence of triphenyltetradecylphosphonium bromide on the Na+-Li+- countertransport rate in the erythrocyte membrane in patients with genetically different permeability of cell membranes to sodium

2012 ◽  
Vol 93 (5) ◽  
pp. 789-791
Author(s):  
O V Orlova ◽  
V N Oslopov ◽  
S A Sidullina
Keyword(s):  
Membrane Permeability ◽  
Erythrocyte Membrane ◽  
Cell Membranes ◽  
Initial Permeability ◽  
Transport Rate ◽  
Low Permeability ◽  
High Permeability ◽  
Initial Membrane ◽  
Genetically Determined ◽  
Erythrocyte Membrane Permeability

Aim. To study the influence of triphenyltetradecylphosphonium bromide [(PPh)3P+C14H29] Br- on the cell membranes permeability to Na+ by determining the rate of Na+-Li+-counter transport in erythrocyte membrane depending on it’s variable initial condition. Methods. Blood samples of 10 healthy volunteers with different Na+-Li+-counter transport rate distribution in erythrocyte membrane were analyzed: I quartile (5 subjects) - low permeability, III quartile (5 subjects) - moderately high permeability. Results. Na+-Li+-counter transport rate change in erythrocyte membrane under the influence of triphenyltetradecylphosphonium bromide depends on genetically determined initial membrane permeability. In subjects belonging to III quartile of Na+-Li+-counter transport the initial membrane permeability is high, and after administration of 0.001 and 0.005 μM concentrations of [(PPh)3P+C14H29] Br- membrane permeability to Na+ increased. In subjects belonging to I quartile of Na+-Li+-counter transport (low initial permeability) [(PPh)3P+C14H29] Br- did not alter membrane permeability to Na+. Conclusion. Triphenyltetradecylphosphonium bromide increases erythrocyte membrane permeability to Na+ in subjects belonging to III quartile of Na+-Li+-counter transport and dose not influence subjects belonging to I quartile of Na+-Li+-counter transport.

Human Erythrocyte Membrane Permeability and Nitroxyl Spin-Label Reduction

1986 ◽  
Vol 75 (4) ◽  
pp. 334-337 ◽  
Author(s):  
Ulf G. Eriksson ◽  
Thomas N. Tozer ◽  
George Sosnovsky ◽  
Jan Lukszo ◽  
Robert C. Brasch
Keyword(s):  
Membrane Permeability ◽  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Spin Label ◽  
Human Erythrocyte Membrane ◽  
Erythrocyte Membrane Permeability

scholarly journals Changes in erythrocyte membrane permeability induced by verapamil, chlorpromazine, and their combinations with amphotericin B

2011 ◽  
Vol 48 (2) ◽  
pp. 225-241 ◽  
Author(s):  
Agnieszka Knopik-Skrocka ◽  
Józef Bielawski ◽  
Szymon Chowański ◽  
Paulina Walkowiak
Keyword(s):  
Membrane Permeability ◽  
Amphotericin B ◽  
Erythrocyte Membrane ◽  
Hemolytic Activity ◽  
Divalent Cations ◽  
Polyene Antibiotic ◽  
Erythrocyte Suspension ◽  
Sucrose Medium ◽  
Erythrocyte Membrane Permeability ◽  
Evident Difference

Changes in erythrocyte membrane permeability induced by verapamil, chlorpromazine, and their combinations with amphotericin BHemolysis induced by 2 amphipathic agents, verapamil and chlorpromazine, was investigated in various incubation conditions. Changes in absorbance of erythrocyte suspension were monitored by absorption spectrophotometry at a wavelength of 590 nm. The hemolysis induced by verapamil or chlorpromazine is of the permeability type. The resistance of erythrocytes to verapamil is much higher than their resistance to chlorpromazine. No evident difference is found between human and pig erythrocytes in their resistance to verapamil. Only a small decrease in the rate of hemolysis induced by verapamil is observed in isotonic CaCl2, MgCl2or K2SO4solutions, compared to 160 mM KCl (the standard incubation medium). The changes in hemolytic activity of chlorpromazine in the presence of the divalent cations and anions are less evident. No decrease in hemolytic activity of chlorpromazine and verapamil is observed in the sucrose medium. The hemolytic activity of both the agents increases when they act in combination with polyene antibiotic amphotericin B. The results indicate a strong synergy between amphotericin B and verapamil or chlorpromazine. By contrast, a combined effect of verapamil and chlorpromazine on erythrocytes leads to a decrease in their hemolytic activity. This indicates antagonism between verapamil and chlorpromazine.

scholarly journals The effect of sorbents on the serum levels of copper, iron and their transporting proteins at alcohol intoxication

2015 ◽  
Vol 96 (5) ◽  
pp. 868-871
Author(s):  
N A Terekhina ◽  
E V Zhidko ◽  
G A Terekhin ◽  
A G Orbidans
Keyword(s):  
Alcohol Abuse ◽  
Membrane Permeability ◽  
Erythrocyte Membrane ◽  
Alcohol Intoxication ◽  
Serum Levels ◽  
Ethanol Intoxication ◽  
Ceruloplasmin Level ◽  
Acute Ethanol ◽  
Acute Ethanol Intoxication ◽  
Erythrocyte Membrane Permeability

Aim. Evaluate the effect of sorbents on erythrocyte membrane permeability and serum levels of copper, iron and their transporting proteins at acute ethanol intoxication. Methods. The study was performed on 94 rats. Acute alcohol intoxication was simulated on intact animals and in animals with prior artificial alcohol abuse. Acute ethanol intoxication was caused by intragastric administration of 40% ethanol at a dose of 0.5 of median lethal dose. Polysorb, Litovit, and Sapropel sorbents were administered at a dose of 3000 mg/kg 30 minutes after ethanol administration. permeability of erythrocyte membrane, serum levels of copper, iron, ceruloplasmin and transferrin were measured by spectrophotometry. Results. Levels of copper and iron in rat serum and erythrocyte membrane permeability significantly dropped compared to the control level at acute ethanol intoxication, ceruloplasmin level raised by 1.5 times, transferrin level did not change significantly. At acute ethanol intoxication in animals with prior artificial alcohol abuse, copper and iron levels and erythrocyte membrane permeability remained low, ceruloplasmin level remained high, transferrin level was decreased for 2 times. All sorbents were able to compensate the serum levels of copper, iron and ceruloplasmin in animals with prior artificial alcohol abuse, and Litovit and Polysorb also influenced on transferrin level. Conclusion. Compensatory effect of Polysorb, Litovit, and Sapropel on the serum levels of ceruloplasmin, copper, iron and transferrin and on erythrocyte membrane permeability was discovered at acute ethanol intoxication in animals with prior artificial alcohol abuse.

scholarly journals Effects of triphenyltetradecylphosphonium bromide and tributylhexadecylphosphonium bromide on cellular permeability in patients with hereditary cellular membrane hyperpermeability

2014 ◽  
Vol 95 (1) ◽  
pp. 59-62
Author(s):  
O V Orlova ◽  
V N Oslopov ◽  
S A Sidullina
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Erythrocyte Membrane ◽  
Biologically Active ◽  
Cell Membrane Permeability ◽  
Speed Increase ◽  
Antifungal Action ◽  
Antibacterial And Antifungal ◽  
Genetically Determined

Aim. Comparative analysis of effects of novel biologically active agents: triphenyltetradecylphosphonium bromide and tributylhexadecylphosphonium bromide on cell membrane permeability for sodium by determination of of Na +-Li +-countertransport speed in erythrocyte membrane at patients with genetically determined high membrane permeability for sodium. Methods. Blood samples of 8 healthy volunteers who were classified as persons belonging to IV population quartile according to Na +-Li +-counter-transport speed in erythrocyte membrane, i.e. persons with high membrane permeability, were studied. Effects of different concentrations of triphenyltetradecylphosphonium bromide and tributylhexadecylphosphonium bromide (known by antibacterial and antifungal action) on Na +-Li +-counter-transport speed in erythrocyte membrane in vitro according to the method proposed by M. Canessa et al. Results. Effect of triphenyltetradecylphosphonium bromide (С 14) and tributylhexadecylphosphonium bromide (С 16) on cell membrane permeability for sodium depends on the genetically determined baseline cell membrane state. С 14reduced the erythrocyte membrane permeability for sodium in studied patients belonging to IV quartile of Na +-Li +-counter-transport speed if administered in a concentration of 0.05 μm. C 16increased membrane permeability for sodium in the same group if administered in concentrations of 0.001 and 0.005 μm. Thus, tributylhexadecylphosphonium bromide is better suitable for designing a drug with antibacterial and antifungal action for patients belonging to IV quartile of Na +-Li +-counter-transport speed, if Na +-Li +-counter-transport speed reduction is wanted. If Na +-Li +-counter-transport speed increase is wanted, triphenyltetradecylphosphonium bromide is better suitable. Conclusion. Cand Csubstances affect cell membrane permeability for sodium in patients with genetically determined high membrane permeability for sodium.

Erythrocyte membrane permeability for a series of diols

2004 ◽  
Vol 62 (2) ◽  
pp. 115-118 ◽  
Author(s):  
O.I. Gordiyenko ◽  
T.P. Linnik ◽  
E.O. Gordiyenko
Keyword(s):  
Membrane Permeability ◽  
Erythrocyte Membrane ◽  
Erythrocyte Membrane Permeability

scholarly journals Changes of the erythrocyte membrane permeability for potassium under the influence of radiation

1994 ◽  
Vol 10 (1) ◽  
pp. 90-93
Author(s):  
V. D. Krupin ◽  
G. P. Gorbenko ◽  
S. A. Kurilko ◽  
V. N. Tkachenko ◽  
V. V. Tovstyak
Keyword(s):  
Membrane Permeability ◽  
Erythrocyte Membrane ◽  
Erythrocyte Membrane Permeability

scholarly journals Transient changes in erythrocyte membrane permeability are induced by sublytic amounts of the complement membrane attack complex (C5b-9)

Blood ◽  
1993 ◽  
Vol 81 (1) ◽  
pp. 200-205 ◽  
Author(s):  
JA Halperin ◽  
A Taratuska ◽  
M Rynkiewicz ◽  
A Nicholson-Weller
Keyword(s):  
Membrane Permeability ◽  
Erythrocyte Membrane ◽  
Membrane Attack Complex ◽  
Autologous Serum ◽  
Regulatory Function ◽  
K Channel ◽  
Adaptive Responses ◽  
Membrane Hyperpolarization ◽  
Erythrocyte Membrane Permeability ◽  
Na Uptake

We have previously shown that sublytic heterologous complement induces large but transient increases in erythrocyte membrane permeability. We now report that when erythrocytes are bystanders in zymosan-activated autologous serum, they increase their Na+ permeability 10-fold, indicating that autologous complement can also induce transient membrane lesions. When we isolated the effect of the C5b-9 membrane attack complex of complement by using human C5b-9 assembled from purified components, we found there was minimal lysis but efficient Na+ uptake. Suspension of the sublytically damaged erythrocytes in K+ medium caused the cells to lyse, which is consistent with the cells recruiting a compensatory K+ efflux similar to that observed when human erythrocytes were exposed to heterologous complement. Sublytic C5b-9 exposure also became lytic when extracellular Ca2+ was limited and when the cells were exposed to charybdotoxin, an inhibitor of the Ca(2+)- activated K+ channel. This indicates that Ca2+ is required for the functional termination of the C5b-9 lesion. We also show that the membrane hyperpolarization resulting from activation of the Ca(2+)- dependent K+ efflux does not influence the termination of the C5b-9 lesion. Thus, the influx of Ca2+ through the complement lesion initiates at least two apparently independent adaptive responses: (1) a process that terminates the leak; and (2) a K+ efflux that has a volume regulatory function. Our data support the potential of the sublytic C5b- 9 lesion to act as a physiologic mediator for autologous erythrocytes.

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