scholarly journals On changes in the biochemical properties of tubercle bacillus due to its dissociation

2021 ◽  
Vol 30 (9) ◽  
pp. 884-891
Author(s):  
B. L. Mazur ◽  
A. A. Baev
Keyword(s):  
Biochemical Properties ◽  
Tubercle Bacillus ◽  
Main Hypothesis ◽  
Microbial Species ◽  
Enormous Amount

On the question of the variability of microbes, since 1888, an enormous amount of material has been accumulated. Hadley (1927), in his monograph, brilliantly summarized all the facts, observations and opinions and subordinated them to the main hypothesis, which is an answer to the question about the essence of the very process of variability. Namely: according to Hadley, each microbial species undergoes a certain cycle of development during its life (dissociation); variability is the external expression of this dissociation.

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

Binding of 131I-Labeled Tissue-Type Plasminogen Activator on De-Endothelialized Lesions in Rabbits

1987 ◽  
Vol 26 (05) ◽  
pp. 224-228 ◽  
Author(s):  
Y. Isaka ◽  
H. Etani ◽  
K. Kimura ◽  
S. Yoneda ◽  
T. Kamada ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Abdominal Aorta ◽  
Half Life ◽  
Balloon Catheter ◽  
Biochemical Properties ◽  
Tissue Type ◽  
Fibrin Deposition ◽  
Tissue Type Plasminogen Activator ◽  
High Affinity ◽  
Type Plasminogen Activator

Tissue-type plasminogen activator (t-PA) which has a high affinity for fibrin in the clot, was labeled with 131I by the iodogen method, and its binding to de-endothelialized lesions in the rabbit was measured to assess the detectability of thrombi. The de-endothelialized lesion was induced in the abdominal aorta with a Fogarty 4F balloon catheter. Two hours after the de-endothelialization, 131I-labeled t-PA (125 ± 46 μCi) was injected intravenously. The initial half-life of the agent in blood (n = 12) was 2.9 ± 0.4 min. The degree of binding of 131I-labeled t-PA to the de-endothelialized lesion was evaluated at 15 min (n = 6) or at 30 min (n = 6) after injection of the agent. In spite of the retention of the biochemical properties of 131I-labeled t-PA and the presence of fibrin deposition at the de-endothelialized lesion, the binding of t-PA to the lesion was not sufficiently strong. Lesion-to-control ratios (cpm/g/cpm/g) were 1.65 ± 0.40 (at 15 min) and 1.39 ± 1.31 (at 30 min), and lesion-to-blood ratios were 1.39 ± 0.32 (at 15 min) and 1.36 ± 0.23 (at 30 min). These results suggest that radiolabeled t-PA may be inappropriate as a radiopharmaceutical for the scintigraphic detection of a pre-existing thrombotic lesion.

A Review on Big Data Analytics in Business

2018 ◽  
pp. 210-214
Author(s):  
Manbir Sandhu ◽  
Purnima, Anuradha Saini
Keyword(s):  
Big Data ◽  
Big Data Analytics ◽  
Smart Devices ◽  
Data Streaming ◽  
Huge Amount ◽  
Business Units ◽  
Enormous Amount ◽  
Tools And Techniques ◽  
Heath Care ◽  
Data Analytic

Big data is a fast-growing technology that has the scope to mine huge amount of data to be used in various analytic applications. With large amount of data streaming in from a myriad of sources: social media, online transactions and ubiquity of smart devices, Big Data is practically garnering attention across all stakeholders from academics, banking, government, heath care, manufacturing and retail. Big Data refers to an enormous amount of data generated from disparate sources along with data analytic techniques to examine this voluminous data for predictive trends and patterns, to exploit new growth opportunities, to gain insight, to make informed decisions and optimize processes. Data-driven decision making is the essence of business establishments. The explosive growth of data is steering the business units to tap the potential of Big Data to achieve fueling growth and to achieve a cutting edge over their competitors. The overwhelming generation of data brings with it, its share of concerns. This paper discusses the concept of Big Data, its characteristics, the tools and techniques deployed by organizations to harness the power of Big Data and the daunting issues that hinder the adoption of Business Intelligence in Big Data strategies in organizations.

The Evolution of Psychoanalytic Practice with Psychotic Patients

2002 ◽  
Vol 4 (1) ◽  
pp. 31-43
Author(s):  
Sophie de Mijolla-Mellor
Keyword(s):  
Special Kind ◽  
Main Hypothesis ◽  
Actual Case ◽  
Definition Of ◽  
Psychoanalytic Method

This paper deals with the evolution of the psychoanalytic practice with psychotic patients beginning with Freud's scepticism about the transference capacities of those patients to a new definition of a special kind of psychotic transference. The main hypothesis is that the actual case of psychotics within a psychoanalytic cure has modified the psychoanalytic method itself, even in the field of neuroses. Within the framework and, more specifically, in the case of schizophrenics, this paper develops some reflections on the evolution of the three following concepts: transference/countertransference, communication and interpretation, and reality.

Application of commercial test-systems to identify gram-negative facultatively anaerobic bacteria

2016 ◽  
Vol 3 (1) ◽  
pp. 43-48 ◽  
Author(s):  
V. Patyka ◽  
L. Butsenko ◽  
L. Pasichnyk
Keyword(s):  
Erwinia Amylovora ◽  
Anaerobic Bacteria ◽  
Biochemical Properties ◽  
Test System ◽  
Phytopathogenic Bacteria ◽  
Gram Negative ◽  
Test Systems ◽  
Facultatively Anaerobic ◽  
Microbiological Methods

Aim. To validate the suitability of commercial API 20E test-system (bioMerieux) for the identifi cation and characterization of facultative gram-negative phytopathogenic bacterial isolates. Methods. Conventional mi- crobiological methods, API 20E test-system (bioMerieux) according to the manufacturer’s instructions. Re- sults. The identifi cation results for Erwinia amylovora, Pectobacterium carotovorum and Pantoea agglome- rans isolates were derived from the conventional and API 20E test systems, which, were in line with the literature data for these species. The API 20E test-system showed high suitability for P. agglomerans isolates identifi cation. Although not all the species of facultatively anaerobic phytopathogenic bacteria may be identi- fi ed using API 20E test-system, its application will surely allow obtaining reliable data about their physiologi- cal and biochemical properties, valuable for identifi cation of bacteria, in the course of 24 h. Conclusions. The results of tests, obtained for investigated species while using API 20E test-system, and those of conventional microbiological methods coincided. The application of API 20E test-system (bioMerieux) ensures fast obtain- ing of important data, which may be used to identify phytopathogenic bacteria of Erwinia, Pectobacterium, Pantoea genera.

Ecological and physiological as well as biochemical properties of representatives of the Genus sedum L.

2014 ◽  
Vol 25 (3-4) ◽  
pp. 24-33
Author(s):  
O. I. Dzjuba ◽  
M. V. Yatsenko
Keyword(s):  
Nitric Oxide ◽  
Cell Line ◽  
Methanol Extract ◽  
Hepatoma Cell ◽  
Ethanol Extract ◽  
Biochemical Properties ◽  
Biologically Active ◽  
Hepatoma Cell Line ◽  
Dependent Manner ◽  
Dose Dependent

The article deals with the history of the study and the current state of research of physiological and biochemical properties of the plant genus Sedum that are useful for human and has been used in folk medicine for many years. It was noticed that antioxidant properties of extracts from plants S. sarmentosum, S. sempervivoides, S. takesimense were caused by the presence of phenolic compounds. Methanol extract of plants S. takesimense exhibited strong scavenging activities against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radicals as well as significant inhibitory effects on lipid peroxidation and low density lipoprotein (LDL) oxidation induced by a metal ion Cu2+. Various immunomodulatory activities of various fractions of plants extracts (S. dendroideum, S. kamtschaticum, S. sarmentosum, S. telephium) are observed. It was shown that the ethanol extract of S. sarmentosum and it’s fractions suppressed specific antibody and cellular responses to ovalbumin in mice. The methanol extract of plants S. sarmentosum reduced the levels of anti-inflammatory markers, such as volume of exudates, number of polymorphonuclear leukocytes, suppressed nitric oxide synthesis in activated macrophages via suppressed induction of inducible nitric oxide synthase (iNOS). Polysaccharides fractions from plants S. telephium inducing productions of tumor necrosis factor alpha (TNF-α), increasing the intensity of phagocytosis in vitro and in vivo. Methanol extract from the whole part of S. kamtschaticum strongly inhibit PGE2 production from lipopolysaccharide-induced RAW 264.7 cells, a mouse macrophage cell line via modulating activity in gene expression of the enzyme cyclooxygenase-2 (COX-2). The methanol extract of plants S. sarmentosum and the major kaempferol glycosides from S. dendroideum have antinociceptive activity. It was noticed that anti-adipogenic activity of extracts from plants S. kamtschaticum were caused by inhibition of peroxisome-proliferator-activated receptor γ (PPARγ) expression and it’s dependent target genes, such as genes encoding adipocyte protein 2 (аР2), lipoprotein lipase (LPL), adiponectin and CD36. Polysaccharides fractions from S. telephium cause inhibition of cell adhesion of human fibroblast (MRC5) to laminin and fibronectin via interfere with integrin-mediated cell behaviour and they contributed to the role of polysaccharides in cell-matrix interaction. The methanol extract of plants S. sarmentosum exhibited a significant inhibitory activity in the chick embryo chorioallantoic membrane angiogenesis in a dose-dependent manner. The crude alkaloid fraction of S. sarmentosum caused a dose-dependent inhibition of cell proliferation on murine hepatoma cell line BNL CL.2 and human hepatoma cell line HepG2 without necrosis or apoptosis. Alkaloids from plants S. sarmentosum may improve survival of hepatoma patients via the inhibition of excessive growth of tumor cells. Plant’s juices have antiviral activity (S. sarmentosum, S. spurium, S. stahlii). Crude ethanol extract S. praealtum have spermicidal activity of the in mice and a relevant inhibitory effect of aqueous extract on human spermatozoa motility as well as an anti-fertilizing activity in rats. Hepatoprotective triterpenes, e.g., δ-amyrone, 3-epi-δ-amyrin, δ-amyrin and sarmentolin were isolated from S. sarmentosum. 2- and 2,6-substituted piperidine alkaloids (e.g., norsedamine, allosedridine, sedamine, allosedamine) are observed in plants S. acre, which in the presence of data on the use of pyridine and piperidine derivatives for treating neurodegenerative diseases (e.g., Alzheimer's disease), points on the promising research in this area. Taking into account that biologically active compounds are accumulated in the aboveground vegetative organs of plants of Sedum, the prospects of further study of the use of Sedum for the purposes of biotechnology and in the pharmaceutical industry becomes apparent. This work extends the existing views regarding the use of plants Sedum.

Statistical Justification of Pearson's Criterion for Testing a Complex Hypothesis on the Uniform Distribution

2018 ◽  
pp. 45-53
Author(s):  
T. V. Oblakova
Keyword(s):  
Uniform Distribution ◽  
Degrees Of Freedom ◽  
Likelihood Function ◽  
Random Number Generator ◽  
Maximum Likelihood Estimates ◽  
Chi Square ◽  
Main Hypothesis ◽  
Pearson Criterion ◽  
Complex Hypothesis ◽  
Uniform Law

The paper is studying the justification of the Pearson criterion for checking the hypothesis on the uniform distribution of the general totality. If the distribution parameters are unknown, then estimates of the theoretical frequencies are used [1, 2, 3]. In this case the quantile of the chi-square distribution with the number of degrees of freedom, reduced by the number of parameters evaluated, is used to determine the upper threshold of the main hypothesis acceptance [7]. However, in the case of a uniform law, the application of Pearson's criterion does not extend to complex hypotheses, since the likelihood function does not allow differentiation with respect to parameters, which is used in the proof of the theorem mentioned [7, 10, 11].A statistical experiment is proposed in order to study the distribution of Pearson statistics for samples from a uniform law. The essence of the experiment is that at first a statistically significant number of one-type samples from a given uniform distribution is modeled, then for each sample Pearson statistics are calculated, and then the law of distribution of the totality of these statistics is studied. Modeling and processing of samples were performed in the Mathcad 15 package using the built-in random number generator and array processing facilities.In all the experiments carried out, the hypothesis that the Pearson statistics conform to the chi-square law was unambiguously accepted (confidence level 0.95). It is also statistically proved that the number of degrees of freedom in the case of a complex hypothesis need not be corrected. That is, the maximum likelihood estimates of the uniform law parameters implicitly used in calculating Pearson statistics do not affect the number of degrees of freedom, which is thus determined by the number of grouping intervals only.

Sustainable tourism planning in Bahía Solano, Colombia

2020 ◽  
Vol 10 (2) ◽  
pp. 165-176
Author(s):  
Gabriela Antošová ◽  
Ivan Dario Medina Rojas ◽  
Mauricio Peralta Mejía ◽  
Helmuth Yesid Arias Gómez
Keyword(s):  
Social Innovation ◽  
Local Development ◽  
Sustainable Tourism ◽  
High Rate ◽  
Tourism Planning ◽  
Tourist Attractions ◽  
Marine Area ◽  
Bird Watching ◽  
Enormous Amount ◽  
Water Sports

AbstractThe municipality of Bahía Solano possesses important tourist attractions. The majority of them consist of natural wonders such as whale, dolphin and bird watching. Its marine area offers diving courses, all types of fishing, surfing, other water sports etc. The enormous amount of natural wealth stands in contrast to the lack of local development and the high rate of poverty among its inhabitants. Bahía Solano has enviable tourist professions but a low level of competition. This research proposes a methodology of social innovation, elaborated by researchers, that involves nature, communities and tour operators, where a connection of surveys with the expectations and perceptions of the different entities (inhabitants, tourists, and tour operators) has been obtained.

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