scholarly journals Synthesis of the matrix metalloproteinases and its inhibitors by peripheral blood phagocytes of women with hypertansion disorders

2015 ◽  
Vol 64 (3) ◽  
pp. 26-32 ◽  
Author(s):  
Irina Aleksandrovna Panova ◽  
Anna Ivanovna Malyshkina ◽  
Anna Vladimirovna Kudryashova ◽  
Daria Aleksandrovna Khlipunova ◽  
Elena Arkadyevna Rockatanskaya ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Matrix Metalloproteinases ◽  
Arterial Hypertension ◽  
Mrna Expression ◽  
Peripheral Blood ◽  
Functional Activity ◽  
Rt Pcr ◽  
Significant Elevation ◽  
The Matrix ◽  
Innate Immunity Cells

Development of the hypertension disorders in pregnant women is associated with the increase of the functional activity of innate immunity cells. RT-PCR assessment of the MMP-2 and TIMP-1 synthesis by peripheral blood phagocytes showed the significant elevation of the MMP-9 mRNA expression by neutrophils in women with chronic arterial hypertension (CAH) and TIMP-1 and TIMP-2 mRNA expression by monocytes in women with CAH and preeclampsia.

scholarly journals MMP-10, MMP-7, TIMP-1 and TIMP-2 mRNA expression in esophageal cancer

2017 ◽  
Vol 64 (2) ◽  
Author(s):  
Agnieszka Juchniewicz ◽  
Oksana Kowalczuk ◽  
Robert Milewski ◽  
Wojciech Laudański ◽  
Piotr Dzięgielewski ◽  
...  
Keyword(s):  
Esophageal Cancer ◽  
Matrix Metalloproteinases ◽  
Mrna Expression ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Clinicopathologic Features ◽  
Tumour Stage ◽  
Rt Pcr ◽  
Tissue Inhibitors ◽  
Node Metastasis ◽  
The Matrix

Introduction: Tissue inhibitors of metalloproteinases (TIMP) and the matrix metalloproteinases (MMP) are involved in the spread of cancer. Methods: We have evaluated the matrix metalloproteinases’ (MMP-10, MMP-7) and their inhibitors’ (tissue inhibitors of metalloproteinases – TIMP-1, TIMP-2) mRNA expression in 61 esophageal cancer samples from patients who had undergone surgery, by using real-time quantitative RT-PCR, and correlated the results with the patient clinicopathologic features. Results: MMP-10, MMP-7, TIMP-1, TIMP-2 were overexpressed in 73%, 85%, 55% and 42% of esophageal cancer samples, respectively. The expression of MMP-10, TIMP-1, and TIMP-2 correlated with the tumor size. The MMP-7 overexpression was associated with the tumour stage (I, II vs III, p=0.05) and lymph node metastasis (N0 vs N1, p=0.037). Conclusions: We conclude that in the resected esophageal cancer an increased mRNA expression of MMP-7, MMP-10 and TIMP-1 correlated with clinicopathologic features. We suggest that these genes may play a role during progression of the disease.

Effects of Immunomodulators on Functional Activity of Innate Immunity Cells Infected with Streptococcus pneumoniae

2015 ◽  
Vol 158 (4) ◽  
pp. 461-464
Author(s):  
N. G. Plekhova ◽  
N. M. Kondrashova ◽  
L. M. Somova ◽  
E. I. Drobot ◽  
I. N. Lyapun
Keyword(s):  
Innate Immunity ◽  
Streptococcus Pneumoniae ◽  
Functional Activity ◽  
Innate Immunity Cells

Interleukin-2 immunotherapy action on innate immunity cells in peripheral blood and tumoral tissue of pancreatic adenocarcinoma patients

2008 ◽  
Vol 394 (1) ◽  
pp. 115-121 ◽  
Author(s):  
Luca Degrate ◽  
Cinzia Nobili ◽  
Claudio Franciosi ◽  
Roberto Caprotti ◽  
Fernando Brivio ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Pancreatic Adenocarcinoma ◽  
Peripheral Blood ◽  
Interleukin 2 ◽  
Innate Immunity Cells

Cyclin D1 mRNA Expression In CLL: a Pilot Study.

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 4619-4619
Author(s):  
Heidi Mocikova ◽  
Sona Pekova ◽  
Lenka Zejskova ◽  
Martin Spacek ◽  
Tomas Kozak
Keyword(s):  
Bone Marrow ◽  
Overall Survival ◽  
Prognostic Factors ◽  
Cyclin D1 ◽  
Mrna Expression ◽  
Peripheral Blood ◽  
Newly Diagnosed ◽  
Rt Pcr ◽  
Trisomy 12

Abstract Abstract 4619 Background. Expression of cyclin D1 demonstrated by immunohistochemistry is seen in 95% of mantle cell lymphomas and in other lymphoproliferative diseases including 10% of chronic lymphocytic leukemias (CLL). This study analyzed the impact of cyclin D1 positivity on time to treatment (TTT) and overall survival (OS) measured by RT PCR in newly diagnosed CLL patients and correlation with reported prognostic factors. Patients and methods. Level of cyclin D1 (quantitative real-time RT-PCR with a specific TaqMan flurescent hybridization probe) mRNA expression was analysed in 72 samples (57 peripheral blood and 15 bone marrow) from patients with newly diagnosed CLL. Cyclin D1 expression (cut-off according to ROC curve >3 over the threshold expression in healthy donors) was reported as positive. Fisher's exact test was used to analyze the relationship of cyclin D1 positivity and prognostic factors: del17p, del11q, unmutated IgVH, trisomy 12, ZAP 70 and CD38 positivity, elevated B2microglobulin, elevated LDH and lymphocyte doubling time (LDT) <6 months. The comparison of time to treatment (TTT) and prognostic factors with cyclin D1 positivity was calculated via Spearman correlation coefficient. Survival curves were calculated by Kaplan-Meier survival analysis and comparison between subgroups was performed by the log-rank test. Results. Cyclin D1 was positive in 29 (40%) CLL patients. Although cyclin D1 was not statistically significant for TTT (P=0,145), a trend was observed, suggesting a negative prognostic impact of cyclin D1 overexpression in CLL. Following variables correlated significantly with TTT: del17p (P=0.037), del11q (P=0.003), unmutated IgVH (P=0.004), trisomy 12(P=0.024), positive CD38 (P=0.014), elevated B2microglobulin (P<0.001), elevated LDH (P<0.001) and LDT <6 months (P<0.001). Del 17p, del 11q, trisomy 12 and elevated B2microglobulin were independent factors for TTT in the multivariate analysis. None of these factors were significant for overall survival due to the short follow-up. Conclusion. Cyclin D1 measured by RT-PCR from peripheral blood or bone marrow has no statistically singificant impact on TTT or OS, though a trend pointing to cyclin D1 overexpression in CLL as a negative prognostic marker can be suggested. This data should be confirmed on a larger cohort of patients with a longer follow-up. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Differential Expression of Neurokinin-1 Receptor by Human Mucosal and Peripheral Lymphoid Cells

2000 ◽  
Vol 7 (3) ◽  
pp. 371-376 ◽  
Author(s):  
Triona Goode ◽  
Joe O'Connell ◽  
Wen-Zhe Ho ◽  
Gerald C. O'Sullivan ◽  
J. Kevin Collins ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Lymphocytes ◽  
Lymphoid Cells ◽  
Receptor Expression ◽  
Rt Pcr ◽  
Peripheral Blood Mononuclear ◽  
Neurokinin 1 Receptor ◽  
Neurokinin 1

ABSTRACT Substance P (SP) has been implicated in peripheral and mucosal neuroimmunoregulation. However, confusion remains regarding immunocyte expression of the receptor for SP, neurokinin-1 receptor (NK-1R), and whether there is differential NK-1R expression in the mucosal versus the peripheral immune system. In the same assay systems, we examined the expression of NK-1R in human lamina propria mononuclear cells (LPMC), peripheral blood mononuclear cells (PBMC), peripheral blood lymphocytes (PBL), monocytes, and monocyte-derived macrophages (MDM). Using standard reverse transcription (RT)-PCR, mRNA expression of both the long and the short isoforms of the NK-1R was evident in LPMC but not in PBMC, PBL, monocytes, or MDM. However, by using nested RT-PCR NK-1R mRNA expression was detected in PBMC, PBL, monocytes, and MDM. This level of expression was found to represent one NK-1R mRNA transcript in >1,000 cells. In contrast, by using competitive RT-PCR we demonstrate that LPMC express a more biologically significant level of eight NK-1R mRNA transcripts per cell. Flow cytometric detection of NK-1R expression at the protein level was evident in LPMC but not in PBMC. These findings illustrate the extreme sensitivity of nested RT-PCR and the advantages of competitive RT-PCR in comparative studies of receptor expression in different cell populations. This study suggests that, under normal conditions, readily detectable expression of NK-1R in human mononuclear cells occurs at the mucosal level rather than in the peripheral circulation.

scholarly journals THE FUNCTIONAL ACTIVITY OF INNATE IMMUNITY CELLS IN BACTERIAL INFECTION ON BACKGROUND OF THERMAL STRESS

2018 ◽  
Vol 8 (1) ◽  
pp. 43-53
Author(s):  
N. G. Plekhova ◽  
L. M. Somova ◽  
E. I. Drobot ◽  
A. V. Lagureva ◽  
I. N. Lyapun ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Thermal Stress ◽  
Bacterial Infection ◽  
Functional Activity ◽  
Innate Immunity Cells

CCL20 Stimulates Proinflammatory Mediator Synthesis in Human Fibroblast-like Synoviocytes Through a MAP Kinase-dependent Process with Transcriptional and Posttranscriptional Control

2011 ◽  
Vol 38 (9) ◽  
pp. 1858-1865 ◽  
Author(s):  
NADA ALAAEDDINE ◽  
GEORGE HILAL ◽  
RAFFIC BADDOURA ◽  
JOHN ANTONIOU ◽  
JOHN A. Di BATTISTA
Keyword(s):  
Mrna Expression ◽  
Human Fibroblast ◽  
Target Genes ◽  
Cartilage Degradation ◽  
Rt Pcr ◽  
Induced Expression ◽  
The Matrix ◽  
Cox 2 ◽  
Factor Α ◽  
Fibroblast Like Synoviocytes

Objective.To compare levels of the chemokine CCL20 and its receptor CCR6 in donor, osteoarthritic (OA), and rheumatoid arthritis (RA) synovium; and to determine the molecular mechanism of cellular activation induced by chemokine/receptor ligation in human fibroblast-like synoviocytes (FLS).Methods.Synovia and isolated FLS from donor, OA, and RA joints were analyzed for CCL20 and CCR6 expression by RT-PCR and immunohistochemistry. The effect of CCL20 on cytokines and mediators of cartilage degradation was examined by PCR for mRNA expression levels and ELISA, and Western blotting for protein. CCL20-dependent transcriptional and posttranscriptional activation of target genes was monitored using reporter constructs and luciferase assays in transfected donor FLS.Results.CCL20 and CCR6 proteins were abundantly expressed in RA synovial lining cells compared to donor or OA synovia as judged by immunohistochemistry. RT-PCR of synovial extracts confirmed the predominance of CCL20/CCR6 mRNA expression in RA synovium. CCL20 mRNA expression was low in donor FLS, but increased dramatically after stimulation with recombinant human (rh) interleukin 1ß (IL-1ß). rhCCL20 increased mRNA and protein expression of COX-2, IL-1ß, tumor necrosis factor-α, IL-6, and the matrix-destructive metalloprotease MMP-3 in donor FLS cultures. High constitutive levels of IL-6 were released from RA synovia; CCL20-induced expression of IL-6 occurred through an NSAID/COXIB-sensitive process. CCL20-induced expression of COX-2 was mediated by a PLCP1/PKCα/MEK1/2/ERK1/2-dependent pathway involving both transcriptional and posttranscriptional mechanisms.Conclusion.CCL20/CCR6 may play an important role in the pathogenesis of RA by assembling the molecular and cellular components orchestrating synovitis.

scholarly journals Expression of Dopamine D1−4 and Serotonin 5-HT1A-3A Receptors in Blood Mononuclear Cells in Schizophrenia

2021 ◽  
Vol 12 ◽  
Author(s):  
Adam Wysokiński ◽  
Elżbieta Kozłowska ◽  
Ewa Szczepocka ◽  
Anna Łucka ◽  
Justyna Agier ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Rt Pcr ◽  
Peripheral Blood Mononuclear ◽  
Schizophrenia Group ◽  
Blood Mononuclear Cells ◽  
Mrna And Protein Expression

Introduction: The aim of this study was to determine the mRNA expression profile of dopamine D1, D2, D3, D4 and serotonin 5-HT1A, 5-HT2A, and 5-HT3A receptors in peripheral blood mononuclear cells (PBMCs) in schizophrenia and the in vitro effect of antipsychotics on the expression of these receptors in PBMCs of healthy subjects.Materials and Methods: Twenty-seven patients with schizophrenia and 29 healthy controls were recruited for the study. All study subjects underwent thorough clinical assessment, including anthropometric and body composition measurements. The expression of mRNA for dopamine D1−4 and serotonin 5-HT1A−3A receptors was measured using quantitative RT-PCR in peripheral blood mononuclear cells. In vitro mRNA and protein expression of these receptors was measured using quantitative RT-PCR and Western Blotting in PBMCs cultured with quetiapine, haloperidol, aripiprazole, risperidone, olanzapine or clozapine at IC50, half of IC50, and one-quarter of IC50 concentrations.Results: The key finding was that the schizophrenia group demonstrated significantly higher mRNA expression of D1, D2 and D4 receptors (p &lt; 0.001), and significantly lower mRNA expression of 5-HT3A receptors (p &lt; 0.01). After adjusting for smoking, the mRNA expression of D1 lost its significance, while that of D3, 5-HT1A, 5-HT2A became significant (all three were lower in the schizophrenia group). These receptors also demonstrated different ratios of mRNA expression in the schizophrenia group. The in vitro experiments showed that high concentrations of antipsychotics influenced the mRNA and protein expression of all studied receptors.Conclusion: Schizophrenia patients display a distinctive pattern of dopamine and serotonin receptor mRNA expression in blood mononuclear cells. This expression is little affected by antipsychotic treatment and it may therefore serve as a useful diagnostic biomarker for schizophrenia.

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