DNA damage in placenta and embryos of rats exposed to peat smoke; antigenotoxic effects of afobazole

Dinara M Gorbatova; Aliy K Zhanataev; Helena P Nemova; Andrey D Durnev

doi:10.17816/ecogen14250-56

DNA damage in the placenta and embryos of rats exposed to peat smoke: Antigenotoxic effects of afobazole

Russian Journal of Genetics Applied Research ◽

10.1134/s2079059717060053 ◽

2017 ◽

Vol 7 (6) ◽

pp. 712-716

Author(s):

D. M. Gorbatova ◽

A. K. Zhanataev ◽

E. P. Nemova ◽

A. D. Durnev

Keyword(s):

Dna Damage ◽

Peat Smoke ◽

Antigenotoxic Effects

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Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/5039372 ◽

2019 ◽

Vol 2019 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Lada Živković ◽

Vladan Bajić ◽

Dijana Topalović ◽

Marija Bruić ◽

Biljana Spremo-Potparević

Keyword(s):

Hydrogen Peroxide ◽

Dna Damage ◽

Natural Products ◽

Whole Blood ◽

Blood Cells ◽

Statistical Significance ◽

Individual Treatment ◽

Alternative Source ◽

Whole Blood Cells ◽

Antigenotoxic Effects

The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p<0.05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.

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DNA PROTECTIVE ACTIVITY OF TWO SPECIES OF THE „SCROPHULARIA“ GENUS 2021ICCBIKG (2021)

10.46793/iccbi21.218m ◽

2021 ◽

Author(s):

Sanja Matić ◽

◽

Pavle Mašković ◽

Katarina Šipovac

Keyword(s):

Dna Damage ◽

Plant Species ◽

Biological Activities ◽

Wistar Rat ◽

Negative Control ◽

Control Treatment ◽

Protective Effects ◽

A Value ◽

Antigenotoxic Effects

Plants from the genus Scrophularia, family Scrophulariaceae have numerous biological activities such as antibacterial, antioxidant, antiprotozoal, antitumor, hepatoprotective, and antidiabetic. However, as far as we know, genotoxic and antigenotoxic effects of these two plant species remain unexplored. The present study aimed to evaluate possible in vivo protective effects of the methanol extracts of two plant species of the Scrophularia genus, Scrophularia canina L. and S. alata Gilib., against carbon tetrachloride (CCl4)-induced DNA damage in albino Wistar rat. A significant increase in total comet score has been shown in animals receiving CCl4 compared with the negative control. Treatment with either S. alata or S. canina extracts reduced CCl4 induced DNA damage as indicated by the percentage of reduction in total comet score with a value above 50%.

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In vitro assessment of anticytotoxic and antigenotoxic effects of CANOVA®

Homeopathy ◽

10.1016/j.homp.2016.04.003 ◽

2016 ◽

Vol 105 (03) ◽

pp. 265-269 ◽

Cited By ~ 2

Author(s):

Henrique Fonseca Sousa do Nascimento ◽

Plínio Cerqueira dos Santos Cardoso ◽

Helem Ferreira Ribeiro ◽

Tatiane Cristina Mota ◽

Lorena Monteiro Gomes ◽

...

Keyword(s):

Dna Damage ◽

Human Lymphocytes ◽

Nitroso Compound ◽

Cytoprotective Effect ◽

In Vitro Assessment ◽

Induced Apoptosis ◽

Clinical Conditions ◽

Apoptosis And Necrosis ◽

Antigenotoxic Effects

Background: CANOVA® (CA) is a homeopathic immunomodulator. It contains several homeopathic medicines prepares according to the Brazilian Pharmacopoeia. CA is indicated in clinical conditions in which the immune system is impaired and against tumors. N-methyl-N-nitrosourea (NMU) is an N-nitroso compound, with genotoxic/mutagenic properties. Although several studies have shown promising results in the use of CA, there are no studies reporting possible antigenotoxic effects. Method: This study evaluated the in vitro antigenotoxic and anticytotoxic effects of CA in human lymphocytes exposed to NMU. Samples of human lymphocytes that were subjected to different concentrations of a mixture containing CA and NMU were used. The genotoxicity/antigenotoxicity of CA was evaluated by the comet assay, anticytotoxicity was assessed by quantification of apoptosis and necrosis using acridine orange/ethidium bromide. Results: CA significantly reduced DNA damage induced by NMU and reduced significantly the frequency of NMU-induced apoptosis after 24 h of treatment. Conclusion: CA has an important cytoprotective effect significantly reducing the DNA damage and apoptosis induced by the carcinogen NMU.

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