scholarly journals Transdermal redermalization in rosacea: Rehab procedures in intercurrent period

2020 ◽  
Vol 23 (3) ◽  
pp. 182-187
Author(s):  
Anastasia S. Romashkina ◽  
O. Y. Olisova ◽  
E. S. Snarskaya
Keyword(s):  
Laser Scanning ◽  
High Efficiency ◽  
Sodium Succinate ◽  
Lymphatic Drainage ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Energy Potential ◽  
Innovative Method ◽  
Facial Area

Background: The authors presented their own experiences of skin restoration in patients with rosacea after a course of active therapy using transdermal redermalization. Aim: The course of cosmetic correction procedures using this method aimed to restore the energy potential of cells, water balance, and structures of the dermal elastin fibers, normalize microcirculation processes, and create an optimal physiological environment for the mitotic activity of epidermal cells. Materials and methods: An innovative method of transdermal redermalization represents the administration of a drug containing hyaluronic acid and sodium succinate using the technique of papular intradermal injections, taking into account the peculiarities of lymphatic drainage in the facial area. Results: To assess the therapys efficiency, noninvasive monitoring of changes in morphological structures of the skin was performed using confocal laser scanning microscopy. Conclusions: The authors revealed the high efficiency of the method, which controls the remission state for a long term, subsequently avoiding drug therapy

High Bond Durability of Universal Adhesives on Glass Ceramics Facilitated by Silane Pretreatment

2018 ◽  
Vol 43 (6) ◽  
pp. 602-612 ◽  
Author(s):  
C Yao ◽  
H Yang ◽  
J Yu ◽  
L Zhang ◽  
Y Zhu ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Glass Ceramics ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Single Bond ◽  
Thermal Cycles ◽  
Universal Adhesive ◽  
Universal Adhesives ◽  
Resin Bonding

SUMMARY Objective: This study aimed to investigate the long-term effectiveness of ceramic–resin bonding with universal adhesives in non–silane-pretreated and silane-pretreated modes after 10,000 cycles of thermal aging. Methods and Materials: All Bond Universal, Adhese Universal, Clearfil Universal Bond, and Single Bond Universal were selected. Etched lithium disilicate glass ceramics were prepared, randomly assigned to groups, and pretreated with or without ceramic primer containing silane coupling agent prior to the application of universal adhesive (ie, silane-pretreated or non–silane-pretreated mode). The shear bond strength (SBS), microleakage, and field-emission scanning electron microscopy images of the ceramic–resin interfaces were examined after 24 hours of water storage or 10,000 thermal cycles. Light microscopy and confocal laser scanning microscopy (CLSM) were performed to analyze marginal sealing ability. Results: SBS and microleakage percentage were significantly affected by bonding procedure (non–silane-pretreated or silane-pretreated mode) and aging (24 hours or 10,000 thermal cycles). After the universal adhesives in the non–silane-pretreated mode were aged, SBS significantly decreased and microleakage percentage increased. By contrast, the SBS of Adhese Universal, Clearfil Universal Bond, and Single Bond Universal decreased, and the microleakage percentage of all of the adhesives increased in the silane-pretreated mode. However, after aging, the SBS of the silane-pretreated groups were higher and their microleakage percentages lower than those of the non–pretreated groups. In the non–silane-pretreated mode, adhesive failure was dominant and gaps between composite resin and the adhesive layer were significant when observed with CLSM. Conclusions: The simplified procedure reduced the ceramic–resin bonding effectiveness of universal adhesives after aging, and additional silane pretreatment helped improve the long-term durability.

scholarly journals Cell Adherence and Drug Delivery from Particle Based Mesoporous Silica Films

2019 ◽  
Author(s):  
Emma Björk ◽  
Bernhard Baumann ◽  
Florian Hausladen ◽  
Rainer Wittig ◽  
mika lindén
Keyword(s):  
Drug Delivery ◽  
Laser Scanning ◽  
Single Cells ◽  
Silicon Wafers ◽  
Laser Scanning Microscopy ◽  
Drug Uptake ◽  
C2c12 Cells ◽  
Confocal Laser ◽  
Particle Sizes

Spatially and temporally controlled drug delivery is important for implant and tissue engineering applications, as the efficacy and bioavailability of the drug can be enhanced, and can also allow for drugging stem cells at different stages of development. Long-term drug delivery over weeks to months is however difficult to achieve, and coating of 3D surfaces or creating patterned surfaces is a challenge using coating techniques like spin- and dip-coating. In this study, mesoporous films consisting of SBA-15 particles grown onto silicon wafers using wet processing were evaluated as a scaffold for drug delivery. Films with various particle sizes (100 – 900 nm) and hence thicknesses were grown onto OTS-functionalized silicon wafers using a direct growth method. Precise patterning of the areas for film growth could be obtained by local removal of the OTS functionalization through laser ablation. The films were incubated with the model drug DiO, and murine myoblast cells (C2C12 cells) were seeded onto films with different particle sizes. Confocal laser scanning microscopy (CLSM) was used to study the cell growth, and a vinculin-mediated adherence of C2C12 cells on all films was verified. The successful loading of DiO into the films was confirmed by UV-vis and CLSM. It was observed that the drugs did not desorb from the particles during 24 hours in cell culture. During adherent growth on the films for 4 h, small amounts of DiO and separate particles were observed inside single cells. After 24 h, a larger number of particles and a strong DiO signal were recorded in the cells, indicating a particle mediated drug uptake. A substantial amount of DiO loaded particles were however attached on the substrate after 24 making the films attractive as a long-term reservoir for drugs on e.g. medical implants.<br>

scholarly journals Pericapillary Edema Assessment by Means of the Nailfold Capillaroscopy and Laser Scanning Microscopy

Diagnostics ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1107
Author(s):  
Boris P. Yakimov ◽  
Yury I. Gurfinkel ◽  
Denis A. Davydov ◽  
Anastasia S. Allenova ◽  
Gleb S. Budylin ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Venous Occlusion ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Human Organism ◽  
Infusion Therapy ◽  
Image Sharpness ◽  
Short Term

Edema, i.e., fluid accumulation in the interstitial space, accompanies numerous pathological states of the human organism, including heart failure (HF), inflammatory response, and lymphedema. Nevertheless, techniques for quantitative assessment of the edema’s severity and dynamics are absent in clinical practice, and the analysis is mainly limited to physical examination. This fact stimulates the development of novel methods for fast and reliable diagnostics of fluid retention in tissues. In this work, we focused on the possibilities of two microscopic techniques, nailfold video capillaroscopy (NVC) and confocal laser scanning microscopy (CLSM), in the assessment of the short-term and long-term cutaneous edema. We showed that for the patients with HF, morphological parameters obtained by NVC—namely, the apical diameter of capillaries and the size of the perivascular zone—indicate long-term edema. On the other hand, for healthy volunteers, the application of two models of short-term edema, venous occlusion, and histamine treatment of the skin, did not reveal notable changes in the capillary parameters. However, a significant reduction of the NVC image sharpness was observed in this case, which was suggested to be due to water accumulation in the epidermis. To verify these findings, we made use of CLSM, which provides the skin structure with cellular resolution. It was observed that for the histamine-treated skin, the areas of the dermal papillae become hyporefractive, leading to the loss of contrast and the lower visibility of capillaries. Similar effect was observed for patients undergoing infusion therapy. Collectively, our results reveal the parameters can be used for pericapillary edema assessment using the NVC and CLSM, and paves the way for their application in a clinical set-up.

scholarly journals Hydrosol of Thymbra capitata Is a Highly Efficient Biocide against Salmonella enterica Serovar Typhimurium Biofilms

2016 ◽  
Vol 82 (17) ◽  
pp. 5309-5319 ◽  
Author(s):  
Foteini Karampoula ◽  
Efstathios Giaouris ◽  
Julien Deschamps ◽  
Agapi I. Doulgeraki ◽  
George-John E. Nychas ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Laser Scanning ◽  
High Efficiency ◽  
3D Structure ◽  
Time Lapse ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Planktonic Cells ◽  
Content Type ◽  
Serovar Typhimurium

ABSTRACTSalmonellais recognized as one of the most significant enteric foodborne bacterial pathogens. In recent years, the resistance of pathogens to biocides and other environmental stresses, especially when they are embedded in biofilm structures, has led to the search for and development of novel antimicrobial strategies capable of displaying both high efficiency and safety. In this direction, the aims of the present work were to evaluate the antimicrobial activity of hydrosol of the Mediterranean spiceThymbracapitataagainst both planktonic and biofilm cells ofSalmonella entericaserovar Typhimurium and to compare its action with that of benzalkonium chloride (BC), a commonly used industrial biocide. In order to achieve this, the disinfectant activity following 6-min treatments was comparatively evaluated for both disinfectants by calculating the concentrations needed to achieve the same log reductions against both types of cells. Their bactericidal effect against biofilm cells was also comparatively determined byin situand real-time visualization of cell inactivation through the use of time-lapse confocal laser scanning microscopy (CLSM). Interestingly, results revealed that hydrosol was almost equally effective against biofilms and planktonic cells, whereas a 200-times-higher concentration of BC was needed to achieve the same effect against biofilm compared to planktonic cells. Similarly, time-lapse CLSM revealed the significant advantage of the hydrosol to easily penetrate within the biofilm structure and quickly kill the cells, despite the three-dimensional (3D) structure ofSalmonellabiofilm.IMPORTANCEThe results of this paper highlight the significant antimicrobial action of a natural compound, hydrosol ofThymbra capitata, against both planktonic and biofilm cells of a common foodborne pathogen. Hydrosol has numerous advantages as a disinfectant of food-contact surfaces. It is an aqueous solution which can easily be rinsed out from surfaces, it does not have the strong smell of the essential oil (EO) and it is a byproduct of the EO distillation procedure without any industrial application until now. Consequently, hydrosol obviously could be of great value to combat biofilms and thus to improve product safety not only for the food industries but probably also for many other industries which experience biofilm-related problems.

scholarly journals Cell Adherence and Drug Delivery from Particle Based Mesoporous Silica Films

2019 ◽  
Author(s):  
Emma Björk ◽  
Bernhard Baumann ◽  
Florian Hausladen ◽  
Rainer Wittig ◽  
mika lindén
Keyword(s):  
Drug Delivery ◽  
Laser Scanning ◽  
Single Cells ◽  
Silicon Wafers ◽  
Laser Scanning Microscopy ◽  
Drug Uptake ◽  
C2c12 Cells ◽  
Confocal Laser ◽  
Particle Sizes

Spatially and temporally controlled drug delivery is important for implant and tissue engineering applications, as the efficacy and bioavailability of the drug can be enhanced, and can also allow for drugging stem cells at different stages of development. Long-term drug delivery over weeks to months is however difficult to achieve, and coating of 3D surfaces or creating patterned surfaces is a challenge using coating techniques like spin- and dip-coating. In this study, mesoporous films consisting of SBA-15 particles grown onto silicon wafers using wet processing were evaluated as a scaffold for drug delivery. Films with various particle sizes (100 – 900 nm) and hence thicknesses were grown onto OTS-functionalized silicon wafers using a direct growth method. Precise patterning of the areas for film growth could be obtained by local removal of the OTS functionalization through laser ablation. The films were incubated with the model drug DiO, and murine myoblast cells (C2C12 cells) were seeded onto films with different particle sizes. Confocal laser scanning microscopy (CLSM) was used to study the cell growth, and a vinculin-mediated adherence of C2C12 cells on all films was verified. The successful loading of DiO into the films was confirmed by UV-vis and CLSM. It was observed that the drugs did not desorb from the particles during 24 hours in cell culture. During adherent growth on the films for 4 h, small amounts of DiO and separate particles were observed inside single cells. After 24 h, a larger number of particles and a strong DiO signal were recorded in the cells, indicating a particle mediated drug uptake. A substantial amount of DiO loaded particles were however attached on the substrate after 24 making the films attractive as a long-term reservoir for drugs on e.g. medical implants.<br>

Electroporation-enhanced entrapment of diclofenac sodium and ascorbic acid into DPPC liposomes

2021 ◽  
Vol 16 (11) ◽  
pp. 19-26
Author(s):  
Heba M. Fahmy ◽  
Amani M.R. Idris ◽  
Anwar A. Elsayed ◽  
Ebtesam A. Mohamad
Keyword(s):  
Ascorbic Acid ◽  
Rhodamine B ◽  
Laser Scanning ◽  
High Efficiency ◽  
Diclofenac Sodium ◽  
Particle Size Analysis ◽  
Physical Characteristics ◽  
Laser Scanning Microscopy ◽  
Size Analysis ◽  
Confocal Laser

The effect of electroporation on the physical characteristics of 1,2-dipalmitoyl-Sn-glycerol-3- phosphocholine (DPPC) liposome loading diclofenac sodium, ascorbic acid and Rhodamine B was studied. Group one liposomes were incubated with diclofenac sodium, group two liposomes were incubated with ascorbic acid and group three: liposomes were incubated with Rhodamine B. The three groups were subjected to electroporation protocol. Samples were exposed to different field strengths at pulses 30, 60 and 90 pulses respectively with pulse duration 4 milliseconds. After electroporation, liposomes were separated from unentrapped drugs and form the dye through centrifugation. Physical characteristics of liposomes were examined using Transmission electron microscope (TEM), Confocal laser scanning microscopy (CLSM) and particle size analysis. Liposomes are entrapping ascorbic acid with high efficiency at the values of 900 V/0.2 cm with 60 pulses, 800 and 1000 V/ 0.2 cm at 90 pulses. Diclofenac sodium was entrapped at 800 V/0.2 cm and 90 pulses. Rhodamine B was entrapped at 500 and 1000 V/0.2 cm at 30 pulses. Based on the present results, entrapping of diclofenac sodium and ascorbic acid into liposomes can be controlled efficiently by electroporation technique. Optimum entrapping occurred at 800 V/0.2 cm and 90 pulses for ascorbic acid and DS at 900 V/0.2 cm and 30 pulses for Rh-B.

3-D imaging of cells using a confocal laser scanning microscope and digital image processing

1988 ◽  
Vol 46 ◽  
pp. 96-97
Author(s):  
Thomas M. Jovin ◽  
Michel Robert-Nicoud ◽  
Donna J. Arndt-Jovin ◽  
Thorsten Schormann
Keyword(s):  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Biochemical Processes ◽  
Adjacent Structures

Light microscopic techniques for visualizing biomolecules and biochemical processes in situ have become indispensable in studies concerning the structural organization of supramolecular assemblies in cells and of processes during the cell cycle, transformation, differentiation, and development. Confocal laser scanning microscopy offers a number of advantages for the in situ localization and quantitation of fluorescence labeled targets and probes: (i) rejection of interfering signals emanating from out-of-focus and adjacent structures, allowing the “optical sectioning” of the specimen and 3-D reconstruction without time consuming deconvolution; (ii) increased spatial resolution; (iii) electronic control of contrast and magnification; (iv) simultanous imaging of the specimen by optical phenomena based on incident, scattered, emitted, and transmitted light; and (v) simultanous use of different fluorescent probes and types of detectors.We currently use a confocal laser scanning microscope CLSM (Zeiss, Oberkochen) equipped with 3-laser excitation (u.v - visible) and confocal optics in the fluorescence mode, as well as a computer-controlled X-Y-Z scanning stage with 0.1 μ resolution.

Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

1990 ◽  
Vol 48 (3) ◽  
pp. 168-169
Author(s):  
M. H. Chestnut ◽  
C. E. Catrenich
Keyword(s):  
Acridine Orange ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ulcer Disease ◽  
Gastroduodenal Disease ◽  
H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

Sign in / Sign up

Export Citation Format

Share Document