scholarly journals Fabrication of human Wharton’s jelly extra cellular matrix for tissue engineering

2020 ◽  
Vol 22 (1) ◽  
pp. 124-130
Author(s):  
L I Kalyuzhnaya ◽  
V E Chernov ◽  
A S Frumkina ◽  
S V Chebotarev ◽  
D A Zemlyanoy ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Umbilical Cord ◽  
Cell Adhesion Molecules ◽  
Single Cells ◽  
Sodium Dodecyl ◽  
Human Umbilical Cord ◽  
The Matrix

The development of tissue engineering is based on the use of the extracellular matrix as a construct to which cells migrate and attach for proliferation, differentiation, and long-term functioning. The preparation of the matrix is one of the most important tasks, since it must be non-immunogenic, have optimal mechanical properties, contain cell adhesion molecules and growth factors and degrade at the predicted time. The search for biomaterial for the manufacture of the matrix is limited by a number of circumstances. Tissue-specific for the matrix intravital biomaterial is limited, cadaveric is not acceptable due to age-related changes or diseases that reduce the regenerative capacity of tissues; synthetic materials lack cell adhesion molecules or are not degraded. The umbilical cord is an accessible homologous biomaterial of non- embryonic origin, preserving the features of the embryonic phenotype. The optimal method of decellularization of the Warton jelly of the human umbilical cord in the manufacture of a full-component cell-free matrix is substantiated. Umbilical cord decellularization was carried out using a detergent method with a 0.05% sodium dodecyl sulfate solution for 24 hours. The quality of the decellularization was evaluated microscopically by staining with fluorescent dye and quantification of nucleic acids. The gentle method used to remove cells from the Warton jelly tissue meets the existing criteria for the effectiveness of decellularization, since only single cells and a small amount of deoxyribonucleic acid remain in the processed biomaterial. The technique does not provide centrifugation at high speeds, in which glycosaminoglycans and proteoglycans are lost from the matrix, the enzymatic action that destroys fibrillar collagen structures, and non-physiological conditions of decellularization. The therapeutic success of tissue-engineering structures based on the extracellular matrix will depend not only on the bioactivity of the umbilical cord, but also on the safety of the composition, structure and mechanical characteristics of the matrix. Due to the availability and non-invasiveness of receiving from healthy young donors, provisional organs are an excellent source of homologous biomaterial for matrix production.

scholarly journals Cell adhesion in cancer: Beyond the migration of single cells

2020 ◽  
Vol 295 (8) ◽  
pp. 2495-2505 ◽  
Author(s):  
Michalina Janiszewska ◽  
Marina Candido Primi ◽  
Tina Izard
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Molecular Mechanisms ◽  
Single Cells ◽  
Three Dimensional ◽  
Cellular Adhesion ◽  
Three Dimensional Models ◽  
Cell To Cell Adhesion

Homeostasis in healthy tissues strongly relies on cell-to-cell adhesion and cell-to-extracellular matrix interactions. For instance, normal epithelial cells maintain tissue structure by adhering to each other and to the extracellular matrix. The proteins that mediate these distinct interactions are collectively called cell adhesion molecules and are divided into four major groups: cadherins, integrins, selectins, and immunoglobulins. They not only physically anchor cells, but also critically integrate signaling between the extracellular microenvironment and cells. These signals include biochemical cues, as adhesion proteins can both act as ligand-activated receptors and activate mechanotransduction triggered by changes in the physical environment. Molecular mechanisms related to cell adhesion signaling have been extensively studied, especially because mutations and changes in expression of these proteins, particularly cadherins and integrins, are frequently associated with diseases ranging from developmental intellectual disability to cancer. In fact, two major hallmarks of cancer, loss of cell-to-cell adhesion and anchorage-independent growth, are both dependent on cell adhesion molecules. Despite many studies elucidating the relationships between malignant transformation and metastasis and cellular adhesion processes, several areas still await exploration. Here, we highlight recently discovered roles of adhesion molecules in collective cancer cell migration and discuss the utility of three-dimensional models in studying cell-cell adhesion. We also describe recent therapeutic approaches targeting adhesion molecules.

scholarly journals Growth Factors in Infant Germinal Matrix: Relationship to Extracellular Matrix and Cell Adhesion Molecules

1998 ◽  
Vol 57 (9) ◽  
pp. 858-865
Author(s):  
Yasuhiro Nakamura ◽  
Munehiko Yamamoto ◽  
Sonoe Itoh ◽  
Akiko Haratake ◽  
Yuko Nakano ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Growth Factors ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Germinal Matrix ◽  
Matrix Relationship

scholarly journals Regenerative properties of human extraembryonal organs in tissue engineering

2018 ◽  
Vol 20 (4) ◽  
pp. 192-198
Author(s):  
L I Kalyuzhnaya ◽  
O N Kharkevich ◽  
A A Schmidt ◽  
O V Protasov
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Umbilical Cord ◽  
Current Knowledge ◽  
Biological Properties ◽  
Engineering Structures ◽  
The Matrix ◽  
Cell Growth And Differentiation ◽  
And Function ◽  
Cell Matrix Interactions

The characteristics of the umbilical cord extracellular matrix are discussed relatively of their potential use for tissue engineering. The purpose of this review is to assess the current knowledge about using of homologous biomaterials with regenerative properties to create bioengineered structures. One of the most important components of tissue engineering - matrix (scaffold), resident cells can migrate, attach to it and function. Due to their structure, matrices should be easily integrated into the patient’s tissue and provide conditions for cell growth and differentiation. The cells that populate the matrix in the bioreactor before the transplantation of this construction, or resident cells recruited into the transplanted extracellular matrix), and cell- matrix interactions are absolutely necessary components of tissue engineering. Available commercial bioengineering products made from mammalian tissues have certain advantages and significant disadvantages due to the risks of immunological reactions and transmission of infectious agents. The transplantation of products from xenogenic materials is prohibited by law in the Russian Federation. The donor material is limited, receipt of human cadaver material requires a long period of legal registration, which has a detrimental effect on the biomaterial. Therefore, finding a suitable homologous biomaterial is ongoing. Due to the peculiarities of the embryonic phenotype, extraembryonic tissues have special biological properties, one of which is the scarless healing of wounds. Low immunogenicity, optimal mechanical properties of extracellular matrix, presence of cell adhesion molecules and growth factors promoting regeneration provide anti-inflammatory, anti-fibrotic, anti- scarring properties for tissue engineering structures from umbilical cord and amniotic membrane. Umbilical cord and amnion due to the availability and non-invasiveness of obtaining from healthy young donors are an excellent source of homologous biomaterial for extracting matrices, cells and hydrogels for tissue engineering and regenerative medicine.

A functional extracellular matrix biomaterial enriched with VEGFA and bFGF as vehicle of human umbilical cord mesenchymal stem cells in skin wound healing

2021 ◽  
Author(s):  
Zhongjuan Xu ◽  
Junjun Cao ◽  
Zhe Zhao ◽  
Yong Qiao ◽  
Xingzhi Liu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Limited Resource ◽  
Host Cells ◽  
Endothelial Differentiation ◽  
Human Umbilical Cord ◽  
Skin Wound Healing

Abstract The construction of microvascular network is one of the greatest challenges for tissue engineering and cell therapy. Endothelial cells are essential for the construction of network of blood vessels. However, their application meets challenges in clinic due to the limited resource of autologous endothelium. Mesenchymal stem cells (MSCs) can effectively promote the angiogenesis in ischemic tissues for their abilities of endothelial differentiation and paracrine, and abundant sources. Extracellular matrix (ECM) has been widely used as an ideal biomaterial to mimic cellular microenvironment for tissue engineering due to its merits of neutrality, good biocompatibility, degradability, and controllability. In this study, a functional cell derived ECM biomaterial enriched with VEGFA and bFGF by expressing the collagen-binding domain (CBD) fused factor genes in host cells was prepared. This material could induce endothelial differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) and promote angiogenesis, which may improve the healing effect of skin injury. Our research not only provides a functional ECM material to inducing angiogenesis by inducing endothelial differentiation of hUCMSCs, but also shed light on the ubiquitous approaches to endow ECM materials different functions by enriching different factors. This study will greatly benefit tissue engineering and regenerative medicine researches.

Sign in / Sign up

Export Citation Format

Share Document