To better understand oil and bitumen generation and migration in the Paleogene lacustrine source rocks of the Uinta Basin, Utah, analyses of 182 oil samples and tar-impregnated intervals from 82 core holes were incorporated into a well-established stratigraphic framework for the basin. The oil samples are from the U.S. Geological Survey Energy Resources Program Geochemistry Laboratory Database; the tar-impregnated intervals are from core holes drilled at the Sunnyside and P.R. Spring-Hill Creek tar sands deposits. The stratigraphic framework includes transgressive and regressive phases of the early freshwater to near freshwater lacustrine interval of Lake Uinta and the rich and lean zone architecture developed for the later brackish-to-hypersaline stages of the lake. Two types of lacustrine-sourced oil are currently recognized in the Uinta Basin: (1) Green River A oils, with high wax and low β-carotane contents thought to be generated by source rocks in the fresh-to-brackish water lacustrine interval, and (2) much less common Green River B oils, an immature asphaltic oil with high β-carotane content thought to be generated by marginally mature to mature source rocks in the hypersaline lacustrine interval. Almost all oil samples from reservoir rocks in the fresh-to-brackish water interval are Green River A oils; however four samples of Green River A oils were present in the hypersaline interval, which likely indicates vertical migration. In addition, two samples of Green River B oil are from intervals that were assumed to contain only Green River A oil. Tar sand at the P.R. Spring-Hill Creek deposit are restricted to marginal lacustrine and fluvial sandstones deposited during the hypersaline phase of Lake Uinta, suggesting a genetic relationship to Green River B oils. Tar sand at the Sunnyside deposit, in contrast, occur in marginal lacustrine and alluvial sandstones deposited from the early fresh to nearly freshwater phase of Lake Uinta through the hypersaline phase. The Sunnyside deposit occurs in an area with structural dips that range from 7 to 14 degrees, and it is possible that some tar migrated stratigraphically down section.