Direct Identification of Streptococcus agalactiae in Vaginal Colonization in Pregnant Women Using Polymerase Chain Reaction

2014 ◽  
Vol 5 (4) ◽  
Author(s):  
Rasoul Yousefi Mashouf ◽  
Seyed Masoud Mousavi ◽  
Soghra Rabiee ◽  
Mohammad Yousef Alikhani ◽  
Mohammad Reza Arabestani
Keyword(s):  
Polymerase Chain Reaction ◽  
Pregnant Women ◽  
Streptococcus Agalactiae ◽  
Chain Reaction ◽  
Direct Identification ◽  
Vaginal Colonization ◽  
Polymerase Chain

scholarly journals Antibiotic Susceptibility Patterns and Prevalence of Streptococcus Agalactiae Rectovaginal Colonization Among Pregnant Women in Iran

2020 ◽  
Vol 42 (08) ◽  
pp. 454-459
Author(s):  
Mina Dashtizade ◽  
Mohammad Reza Zolfaghari ◽  
Masoud Yousefi ◽  
Ali Nazari-Alam
Keyword(s):  
Polymerase Chain Reaction ◽  
Pregnant Women ◽  
Streptococcus Agalactiae ◽  
Neonatal Infection ◽  
Resistance Pattern ◽  
Chain Reaction ◽  
Isolation And Identification ◽  
Invasive Infections ◽  
Polymerase Chain ◽  
Susceptibility Patterns

Abstract Objective Streptococcus agalactiae is an important pathogen in neonates and pregnant women. Neonatal invasive infections due to S. agalactiae are life-threatening and preventive strategies for this challenge of human have become a concern. The aim of the present study was to determine the prevalence of rectovaginal colonization, related risk factors and antibiotic resistance pattern of S. agalactiae among pregnant women in Iran. Methods The present study was performed on 240 pregnant women. Vaginal and rectal swabs were obtained from all of the women and then were transferred to the laboratory. The isolation and identification of S. agalactiae was performed by standard microbiological tests and polymerase chain reaction (PCR) assay. The antimicrobial susceptibility patterns of the isolates were determined by the Kirby-Bauer disk diffusion. Polymerase chain reaction was used to detect ermB and mefA genes in erythromycin-nonsusceptible isolates. Results Out of 240 pregnant women, 16 cases (6.7%) were colonized by S. agalactiae. There is no significant association between demographic-obstetric factors and maternal S. agalactiae colonization in the pregnant women. Linezolid, vancomycin and ampicillin were the most effective antibiotics against S. agalactiae. The ermB gene was present in 6 (35.29%) S. agalactiae isolates. However, the mefA gene was not detected in any of the isolates. Conclusion Given the relatively significant prevalence of S. agalactiae colonization in the pregnant women in the present study and the risk of serious neonatal infections, the screening of pregnant mothers for the bacteria seems necessary. Our findings highlight the importance of appropriate antibiotic prophylaxis during pregnancy for the prevention of early onset S. agalactiae-neonatal infection and comorbidity.

scholarly journals Streptococcus agalactiae mastitis of bovine detection by Polymerase Chain Reaction (PCR) test in AL-Diwanyia province

2013 ◽  
Vol 12 (2) ◽  
pp. 101
Author(s):  
Gh. K. A. Al-kuzaay ◽  
Q. H. Kshash
Keyword(s):  
Polymerase Chain Reaction ◽  
Streptococcus Agalactiae ◽  
Specific Primer ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Milk Samples ◽  
Bacteriological Testing ◽  
Polymerase Chain ◽  
Pcr Test

This study was conducted for exam 348 milk samples from (clinically mastitic and other healthy cows) in many areas in AL-Diwanyia province by using CMT and bacteriological testing , which appeared that (64.9%) as percentage of mastitis ( clinically 15.9% , subclinically 84.0% ) Streptococcus agalactiae mastitis 13.2% ( 26.6% clinically , 73.3 % subclinicaly) diagnose by PCR assay by using specific primer (16SrRNA). Streptococcus agalactiae (30 isolates) after classical methods applied for streptococcus agalactiae identification (86 isolates).

scholarly journals How Hidden Can Malaria Be in Pregnant Women? Diagnosis by Microscopy, Placental Histology, Polymerase Chain Reaction and Detection of Histidine-Rich Protein 2 in Plasma

2012 ◽  
Vol 54 (11) ◽  
pp. 1561-1568 ◽  
Author(s):  
Alfredo Mayor ◽  
Laura Moro ◽  
Ruth Aguilar ◽  
Azucena Bardají ◽  
Pau Cisteró ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pregnant Women ◽  
Chain Reaction ◽  
Polymerase Chain
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