scholarly journals Microfluidic isolation of endothelial progenitor cells for vascular tissue engineering

2014 ◽  
Author(s):  
Adam Hatch
Keyword(s):  
Tissue Engineering ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Endothelial Progenitor

scholarly journals In vitro cytocompatibility evaluation of collagen based scaffolds using human endothelial progenitor cells for vascular tissue engineering

2014 ◽  
Vol 1 (1-4) ◽  
pp. 10-16 ◽  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Human Umbilical Cord ◽  
Collagen Scaffolds ◽  
Endothelial Progenitor

Vascular tissue engineering attempts to grow blood vessels through the use of different scaffolds that allows vascular cells such as endothelial cells to form networks and organized in vascular tissue. Various biomaterials are used to produce scaffolds that allow growth and differentiation of stem cells; depending on the cell type and applications some materials are more suitable than other. The aim of this study was to evaluate the cytocompatibility of collagen based scaffolds and to assess the capacity of endothelial progenitor cells (EPC) isolated from human umbilical cord to form vascular networks on these scaffolds. Our results show that after 5 days in culture with collagen scaffolds, the EPC remained viable, a sign of biocompatibility with the 3D scaffolds. Scanning electron microscopy showed that in the collagen scaffolds EPC organize within networks and presents an abundant extracellular matrix that strengthen the links between them. When EPC were cultured on collagenchitosan scaffolds, they are more adherent to the scaffolds compared with collagen, exibiting a good capacity to form networks. This study shows that the collagen and collagen-chitosan scaffolds are not cytotoxic for EPC and they provide the possibility of being used in vascular tissue engineering to help creating blood vessels.

miR-126 modulates angiogenic growth parameters of peripheral blood endothelial progenitor cells

2015 ◽  
Vol 396 (3) ◽  
pp. 245-252 ◽  
Author(s):  
Sebastian M. Goerke ◽  
Lena S. Kiefer ◽  
G. Björn Stark ◽  
Filip Simunovic ◽  
Günter Finkenzeller
Keyword(s):  
Tissue Engineering ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Growth Parameters ◽  
Therapeutic Option ◽  
Tube Formation ◽  
Loss Of Function ◽  
Endothelial Progenitor

Abstract Vascularization plays an important role in tissue engineering applications. It is known that implantation of differentiated endothelial cells or endothelial progenitor cells (EPCs) from cord blood (cbEPCs) gives rise to the formation of a complex functional neovasculature, whereas EPCs isolated from peripheral blood (pbEPCs) have a limited capability to form blood vessels upon implantation. MicroRNA-126 (miR-126) has been shown to have pro-angiogenic effects in vivo. In this study, we investigated whether modulation of miR-126 expression in pbEPCs may alter their angiogenic properties. Gain of function and loss of function experiments revealed that miR-126 has anti-angiogenic effects in pbEPCs. Overexpression of miR-126 resulted in decreased proliferation, migration, invasion and tube formation, while inhibition of miR-126 induced the opposite effects. However, modulation of miR-126 expression did not influence apoptotic susceptibility of pbEPCs. This study provides evidence that inhibition of miR-126 improves angiogenesis-related growth parameters in pbEPCs and may represent a therapeutic option to ameliorate the angiogenic and vasculogenic properties of pbEPCs.

scholarly journals Immortalized Functional Endothelial Progenitor Cell Lines from Umbilical Cord Blood for Vascular Tissue Engineering

2012 ◽  
Vol 18 (11) ◽  
pp. 890-902 ◽  
Author(s):  
Praveen K. Sobhan ◽  
Mahendra Seervi ◽  
Jeena Joseph ◽  
Saneesh Varghese ◽  
Prakash Rajappan Pillai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cord Blood ◽  
Cell Lines ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Endothelial Progenitor

Human endothelial progenitor cell attachment to polysaccharide-based hydrogels: A pre-requisite for vascular tissue engineering

2007 ◽  
Vol 18 (2) ◽  
pp. 339-345 ◽  
Author(s):  
Noélie-Brunehilde Thébaud ◽  
Dorothée Pierron ◽  
Reine Bareille ◽  
Catherine Le Visage ◽  
Didier Letourneur ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Vascular Tissue ◽  
Cell Attachment ◽  
Vascular Tissue Engineering ◽  
Endothelial Progenitor

Labeling and Qualification of Endothelial Progenitor Cells for Tracking in Tissue Engineering: An in Vitro Study

2015 ◽  
Vol 38 (4) ◽  
pp. 224-232 ◽  
Author(s):  
Noélie B. Thébaud ◽  
Audrey Aussel ◽  
Robin Siadous ◽  
Jérome Toutain ◽  
Reine Bareille ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Endothelial Progenitor

The Use of Endothelial Progenitor Cells for Prevascularized Bone Tissue Engineering

2009 ◽  
Vol 15 (8) ◽  
pp. 2015-2027 ◽  
Author(s):  
Jeroen Rouwkema ◽  
Peter E. Westerweel ◽  
Jan de Boer ◽  
Marianne C. Verhaar ◽  
Clemens A. van Blitterswijk
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Progenitor Cells ◽  
Bone Tissue ◽  
Endothelial Progenitor Cells ◽  
Endothelial Progenitor
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