scholarly journals Genetic characterization of the preimplantation embryo development (PED) gene using the congenic B6.K1 and B6.K2 strains of mice

2008 ◽  
Author(s):  
Michael J. Byrne
Keyword(s):  
Embryo Development ◽  
Genetic Characterization ◽  
Preimplantation Embryo ◽  
Preimplantation Embryo Development ◽  
Ped Gene

scholarly journals Influence of the Preimplantation Embryo Development (Ped) Gene on Embryonic Platelet-Activating Factor (PAF) Levels

2006 ◽  
Vol 23 (6) ◽  
pp. 269-273 ◽  
Author(s):  
Elissa T. Purnell ◽  
Carol M. Warner ◽  
Hilton I. Kort ◽  
Dorothy Mitchell-Leef ◽  
Carlene W. Elsner ◽  
...  
Keyword(s):  
Embryo Development ◽  
Preimplantation Embryo ◽  
Platelet Activating Factor ◽  
Preimplantation Embryo Development ◽  
Ped Gene

scholarly journals 232SEARCH FOR THE BOVINE HOMOLOG OF THE MURINE PED GENE

2004 ◽  
Vol 16 (2) ◽  
pp. 237
Author(s):  
T. Fair ◽  
A. Gutierrez-Adan ◽  
M. Murphy ◽  
D. Rizos ◽  
F. Martin ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Embryo Development ◽  
Relative Abundance ◽  
Preimplantation Embryo ◽  
Transcript Abundance ◽  
Preimplantation Embryo Development ◽  
Relative Transcript Abundance ◽  
Ped Gene

The aim of the current study was to identify the bovine homolog of the murine Ped (preimplantation embryo development) gene, which regulates mouse preimplantation embryonic growth, including cleavage rate and embryo survivability, and to characterize the expression pattern of this gene during bovine preimplantation embryo development. Experiment (I): The National Center for Biotechnology Information (NCBI) GenBank/EBI EST databases were searched for bovine-expressed sequence tags (EST) that were homologous with the murine Ped gene (Accession number: NM_010394). The resulting ESTs were aligned and assembled in to one complete sequence (841bp), which was shown to be homologous with the Murine Ped gene and the Bovine Major Histocompatibility Complex class I 4221.1 gene (Accession No.: AJ010865, length 1090bp). The expression of the protein product of the Ped gene by bovine tissue was confirmed using Western Blot analysis. Experiment (II): The expression pattern of the Ped gene homolog during in vivo and in vitro bovine preimplantation embryo development was characterized using real time PCR. Embryos at the same stage for age were compared (Day 1: 2-cell; Day 2: 4-cell; Day 3: 8-cell; Day 4: 16-cell; Day 5: early morula;; Day 6, compact/late morula;; Day 7: blastocyst). The relative transcript abundance was consistently lower in the in vitro-cultured embryos at all stages of preimplantation development the differences were significant on Days 2, 4, 5, 6, and 7. The relative transcript abundance was significantly lower on Days 1, 2, and 3 of in vivo culture than on Days 4, 5, 6, and 7 and was significantly higher in Day 7 blastocysts than in Day 5 early morula. In in vitro-cultured embryos the relative transcript abundance was significantly higher in Day 7 blastocysts compared to all other stages of the preimplantation period. Experiment (III): A quantitative analysis of the Ped gene homolog was carried out on replicates of pools of ten 2-cell embryos collected at 25, 28, 32, and >36hpi from three different fertilizations. Transcript relative abundance was highest in those embryos that had cleaved by 25hpi. By 28hpi abundance had decreased slightly;; as time to cleavage increased further to 32 and >36hpi, the relative abundance decreased significantly. In conclusion, we have successfully identified a potential bovine homolog of the murine Ped gene. Furthermore, we have characterized the expression pattern of this gene during preimplantation embryo development in cattle and we have shown that a greater relative abundance of the gene transcript is associated with embryos of higher quality and greater developmental potential.

scholarly journals X-Linked Huwe1 Is Essential for Oocyte Maturation and Preimplantation Embryo Development

iScience ◽  
2020 ◽  
Vol 23 (9) ◽  
pp. 101523
Author(s):  
Alaa A. Eisa ◽  
Scott Bang ◽  
Katherine J. Crawford ◽  
Emily M. Murphy ◽  
William W. Feng ◽  
...  
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Preimplantation Embryo ◽  
Preimplantation Embryo Development
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