In Vitro Anti-inflammatory Assays on Hexane Extract of Sambong (Blumea balsamifera) Leaves

2017 ◽  
Keyword(s):  
Hexane Extract ◽  
Anti Inflammatory

scholarly journals In vitro antioxidant anti inflammatory and cytotoxicity activities from Hexane extract of Bryonopsis laciniosa fruits.

2018 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Sanjeevkumar C.B ◽  
Ramesh L Londonkar ◽  
Umesh Madire Kattegouda
Keyword(s):  
Cell Line ◽  
Potential Application ◽  
Protein Denaturation ◽  
Cancer Cell Line ◽  
Hexane Extract ◽  
Inflammatory Activity ◽  
Cytotoxicity Activity ◽  
Anti Inflammatory ◽  
Mcf 7

<p><em>Bryonopsis laciniosa </em>also known as “Shivlingi” annual climber with bright red fruits and is reported to be highly medicinal in India<em>. </em>As a folk medicine, the plant is used in treatment of broad range of diseases and disorders. In the present study, Hexane extract of <em>B. laciniosa </em>fruits were used to evaluate <em>in</em> <em>vitro</em> anti inflammatory, antioxidant and Cytotoxicity (towards MCF-7 cell line) activities.<em> In vitro</em> anti inflammatory activity by inhibition of protein denaturation, antioxidant assays like DPPH, ABTS, H<sub>2</sub>O<sub>2</sub> and FRAP were used to measure the antioxidant capacity of the hexane extracts and cytotoxicity activity using MCF-7 breast cancer cell line. Hexane extract showed the effective antioxidant activity in all assays compared to ascorbic acid and BHT. The results for<em> In vitro</em> anti inflammatory activity of hexane extract and Dichlofenac drug were equivalent, hexane extract showed promising activity for inhibition of protein denaturation assay. The cytotoxicity activity from hexane extract was noticeable against MCF-7 cell line. The overall results show potential application of <em>Bryonopsis laciniosa </em>fruits suggesting their potential application as a health-promoting functional ingredient or natural preservative in foods.</p>

scholarly journals In vitro and in vivo investigation of polypharmacology of propolis extract as anticancer, antibacterial, anti-inflammatory, and chemical properties

2021 ◽  
Vol 19 (1) ◽  
pp. 864-874
Author(s):  
Nael Abutaha ◽  
Mohammed AL-Zharani ◽  
Amal Alotaibi ◽  
Mary Anne W. Cordero ◽  
Asmatanzeem Bepari ◽  
...  
Keyword(s):  
Chemical Properties ◽  
Acid Methyl Ester ◽  
Cost Effective ◽  
Skin Inflammation ◽  
Hexane Extract ◽  
Major Constituent ◽  
Propolis Extract ◽  
Anti Inflammatory

Abstract Numerous compounds derived from natural sources such as microbes, plants, and insects have proven to be safe, efficacious, and cost-effective therapeutics for human diseases. This study examined the bioactivities of propolis, a structural sealant and antibacterial/antifungal agent produced by honey bees. Chinese propolis was extracted in methanol or hexane. Propolis significantly reduced the numbers of viable cancer cells when applied as a methanol extract (IC50 values in μg/mL for the indicated cell line: MDA-MB-231, 74.12; LoVo, 74.12; HepG2, 77.74; MCF7, 95.10; A549, 114.84) or a hexane extract (MDA-MB-231, 52.11; LoVo, 45.9; HepG2, 52.11; MCF7, 78.01; A549, 67.90). Hexane extract also induced apoptosis of HepG2 cells according to activated caspase-3/7 expression assays (17.6 ± 2.9% at 150 μg/mL and 89.2 ± 1.9% at 300 μg/mL vs 3.4 ± 0.4% in vehicle control), suppressed the growth of Candida albicans and multiple multidrug-resistant and nonresistant Gram-positive bacteria, and inhibited croton oil-induced skin inflammation when applied as topical treatment. GC-MS identified hexadecanoic acid methyl ester as a major constituent (33.6%). Propolis hexane extract has potential anticancer, antimicrobial, and anti-inflammatory activities.

scholarly journals Phytochemical Characterization, In Vitro Anti-Inflammatory, Anti-Diabetic, and Cytotoxic Activities of the Edible Aromatic Plant; Pulicaria jaubertii

Molecules ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 203
Author(s):  
Hamdoon A. Mohammed ◽  
Mohammed F. Abdelwahab ◽  
El-Sayed M. El-Ghaly ◽  
Ehab A. Ragab
Keyword(s):  
Biological Activities ◽  
Hexane Extract ◽  
Hexane Fraction ◽  
Antidiabetic Activity ◽  
Cytotoxic Activities ◽  
Alcoholic Extract ◽  
Aerial Parts ◽  
Anti Inflammatory ◽  
First Time

Pulicaria jaubertii is a medicinal herb that alleviates inflammations and fever. Chromatographic separation, phytochemical characterization, and in vitro biological activities of the plant n-hexane extract were conducted for the first time in this study. Six compounds were isolated for the first time from the n-hexane fraction of Pulicaria jaubertii aerial parts and were identified on the bases of NMR and MS analyses as pseudo-taraxaterol (1), pseudo-taraxasterol acetate (2), 3β-acetoxytaraxaster-20-en-30-aldehyde (3), calenduladiol-3-O-palmitate (4), stigmasterol (5), and α-tocospiro B (6). Compound (6) was a rare tocopherol-related compound and was isolated for the first time from family Asteraceae, while compound (3) was isolated for the first time from genus Pulicaria. The total alcoholic extract and n-hexane fraction were tested for their anti-inflammatory, antidiabetic, and cytotoxic activities. The n-hexane fraction has dose dependent red blood cells (RBCs) membrane stabilization and inhibition of histamine release activities with IC50: 60.8 and 72.9 µg/mL, respectively. As antidiabetic activity, the alcoholic extract exerted the most inhibition on the activity of yeast α-glucosidase, with an IC50: 76.8 µg/mL. The n-hexane fraction showed cytotoxic activity against hepatocarcinoma (HepG-2), breast carcinoma (MCF-7), and prostate carcinoma (PC-3) cell lines with IC50: 51.8, 90.8 and 62.2 µg/mL, respectively. In conclusion, the anti-inflammatory effect of Pulicaria jaubertii might be attributed to the triterpenoid constituents of the n-hexane extract of the plant.

scholarly journals In Vivo and in Vitro Anti-Inflammatory Activities of Extracts of Pandiaka angustifolia (Vahl.) Hepper (Amaranthaceae) Used in Traditional Medicine in Burkina Faso

2020 ◽  
pp. 101-107
Author(s):  
Emmanuel A. M. Thiombiano ◽  
Mindiédiba Jean Bangou ◽  
Yougbaré-Ziébrou Mouhibatou ◽  
Martin Kiendrebeogo
Keyword(s):  
Aqueous Extract ◽  
Analgesic Activity ◽  
Ethanolic Extract ◽  
Scientific Basis ◽  
Hexane Extract ◽  
K Channel ◽  
Aqueous Extracts ◽  
Anti Inflammatory

Background: Pandiaka angustifolia Valh Hepper (Amaranthaceae) whole plant is used in folk Burkinabe’s medicine to treat ailments with an inflammatory component. Previous studies revealed the antioxidant capacity, xanthine oxidase, and lipoxygenase inhibitory activities of the plant, but to the best of our knowledge, its anti-inflammatory activities were not reported before. Therefore, this study was designed to evaluate the anti-inflammatory and analgesic activity of P. Angustifolia hexane and aqueous extracts using in vitro enzymatic methods and in vivo methods and verify the best anti-inflammatory extract implication in KATP pathways. Experiments: acute toxicity of the plant was conducted under OECD 423 guidelines. Phospholipase and cyclooxygenases were pro-inflammatory enzymes used to evaluate in vitro anti-inflammatory effects of plant extracts while carrageenan induced edema method was used to evaluate the anti-edematous activity and acetic acid inducing writhing method to evaluate the non-morphine analgesic effect of herbal mixture. ATP sensitive K+ channel assay was performed in vivo using the glibenclamide as ATP-sensitive potassium channel (KATP) blocker. Results: enzymatic inhibition assays revealed that both hexane and aqueous extracts of P. angustifolia were good inhibitors against sPLA2 activity with IC50 values of 14.23 ± 0. 72 µg/mL and 11.56 ± 0.11 µg/mL, respectively. Aqueous extract presented the best inhibition for COX-1 (IC50 = 24.76 ±0. 51 µg/mL) while hexane extract concentration that inhibit 50% of COX-2 was lesser than those of aqueous extract. P. angustifolia aqueous extract orally administrated to NMRI mice caused no death at the dose of 3000 mg/kg b.w indicating that the plant toxicity is low. While hexane extract was unable to reduce Carrageenan-induced edema, ethanolic extract were significantly active when extract was orally administrated. Non-morphine analgesic activity evaluation revealed that ethanolic extract was more efficient on writhing reduction than hexane extract. Nociception effect of the plant is linked with its effects on K+ ATP sensitive channels. Conclusion: Results indicate that the anti-inflammatory potential of P. angustifolia may be due to its polar phytoconstituents and observed pharmacological activities provide the scientific basis for the medicinal use of the plant in the treatment of ailment associated with inflammation.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

In vitro and in vivo evaluation of the anti-inflammatory effects of Arzanol from Helichrysum italicum

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
J Bauer ◽  
F Dehm ◽  
A Koeberle ◽  
F Pollastro ◽  
G Appendino ◽  
...  
Keyword(s):  
In Vivo Evaluation ◽  
Anti Inflammatory

In vitro anti-inflammatory and wound healing activities of Citrus auraptene

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
F Epifano ◽  
S Genovese ◽  
L Zhao ◽  
V Dang La ◽  
D Grenier
Keyword(s):  
Wound Healing ◽  
Anti Inflammatory

Evaluation of in vitro anti-inflammatory effects of the remaining water subextract of Cistus laurifolius leaves

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
İ Atay ◽  
AZ İlter ◽  
Y Bağatur ◽  
D Telci ◽  
H Kırmızıbekmez ◽  
...  
Keyword(s):  
Anti Inflammatory
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