Genetic Variability Induction through Gamma Irradiation in Ox-Eye Daisy (Leucanthemum Vulgare Lam.) And Their Genetic Relationship

2016 ◽  
Keyword(s):  
Genetic Variability ◽  
Gamma Irradiation ◽  
Genetic Relationship

scholarly journals Genetic analysis of species in the Genus Catasetum (ORCHIDACEAE) using RAPD Markers

2010 ◽  
Vol 53 (2) ◽  
pp. 375-387 ◽  
Author(s):  
Luciana do Valle Rego Oliveira ◽  
Ricardo Tadeu de Faria ◽  
Claudete de Fátima Ruas ◽  
Paulo Maurício Ruas ◽  
Melissa de Oliveira Santos ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Genetic Analysis ◽  
Genetic Relationship ◽  
Genetic Similarity ◽  
Core Collection ◽  
Rapd Markers ◽  
Dna Amplification ◽  
Breeding Programs

In this work, RAPD molecular markers were used to access the genetic variability and to study the inter and intraespecifc relationship in a group of 37 species, including 56 individuals. A total of 15 RAPD primers were selected for DNA amplification. From a total of 221 bands analyzed, 209 (95%) were polymorphics. The level of interespecifc genetic similarity ranged from 37% between Catasetum complanatum and Catasetum laminatum to 83% between Catasetum triodon and Catasetum uncatum. The intraspecifc genetic similarity varied 88% for the individuals of Catasetum triodon to 93% between the individuals of Catasetum atratum and Catasetum macrocarpum. These results would contribute to understand the genetic relationship in Catasetum, to define the strategies to establish a germplasm core collection for the genus and to provide support for breeding programs.

scholarly journals Radiosensitivitas dan Keragaman Ubi Kayu (Manihot esculenta Crantz) Hasil Iradiasi Sinar Gamma

2015 ◽  
Vol 43 (2) ◽  
pp. 111
Author(s):  
Sadewi Maharani ◽  
Nurul Khumaida ◽  
Muhamad Syukur ◽  
Sintho Wahyuning Ardie
Keyword(s):  
Genetic Variability ◽  
Gamma Irradiation ◽  
Gamma Rays ◽  
Manihot Esculenta ◽  
Potential Candidate ◽  
Manihot Esculenta Crantz ◽  
Induced Mutation ◽  
High Genetic Variability ◽  
Phenotype Variability ◽  
Lethal Doses

<p>ABSTRACT</p><p>High genetic variability is one of important determinants in successful breeding of clonally propagated crops such as cassava. Induced mutation, including mutagenesis using gamma irradiation, is one strategy to increase genetic variability. The objective of this research was to obtain information of lethal doses (LD20-LD50) from five cassava genotypes (Jame-jame, Ratim, UJ-5, Malang-4, and Adira-4), to analyze the variability, and to obtain potentially high yielding cassava mutant candidates. Cuttings of several cassava genotypes were irradiated by 0, 15, 30, 45, and 60 Gy gamma rays. The results showed that genotype Malang-4 had the lowest LD20 (7.53 Gy) and LD50 (18.47 Gy), while genotype Jame-jame had the highest LD20 (24.94 Gy) and LD50 (33.24 Gy). The highest phenotype variability was obtained in the cassava population irradiated by 15-30 Gy gamma rays. The highest phenotype variability was also determined by the source of tissue, i.e., cuttings from the middle stem resulted in the highest phenotype variability. This research found 32 high yielding potential candidate mutants.</p><p>Keywords: gamma rays, LD20, LD50,mutant, mutation</p>

scholarly journals PENGARUH IRADIASI SINAR GAMMA (60Co) TERHADAP PERTUMBUHAN DAN PRODUKSI JAHE PUTIH KECIL (Zingiber officinale var.a ma rum)

2020 ◽  
Vol 21 (2) ◽  
pp. 47
Author(s):  
NURLIANI BERMAWIE ◽  
NUR LAELA W. MEILAWATI ◽  
S. PURWIYANTI ◽  
MELATI MELATI
Keyword(s):  
Genetic Variability ◽  
Gamma Irradiation ◽  
Plant Height ◽  
Irradiation Dose ◽  
Leaf Size ◽  
Zingiber Officinale ◽  
Morphological Characters ◽  
Split Plot ◽  
Qualitative Characters ◽  
Main Plot

<p>ABSTRAK</p><p>Keragaman  genetik  plasma  nutfah  jahe  rendah  sehingga  perlu <br /> dilakukan  peningkatan  keragaman,  antara  lain  dengan  iradiasi sinar <br /> gamma. Penelitian ini bertujuan untuk mengetahui pengaruh dosis iradiasi <br /> terhadap pertumbuhan dan produksi jahe putih kecil.  Penelitian dilakukan <br /> di Laboratorium Balai Penelitian Tanaman Rempah dan Obat serta Kebun <br /> Percobaan Cicurug, Sukabumi pada bulan Juni 2010 sampai dengan Mei <br /> 2011. Iradiasi dilakukan di P3TIR, BATAN, Jakarta. Percobaan disusun <br /> menggunakan rancangan split plot dengan petak utama dua aksesi jahe <br /> putih kecil ZIOF 0048 dan HALINA 1 dan anak petak adalah 11 dosis <br /> iradiasi (0-50 Gy) dengan interval 5 Gy. Perlakuan diulang sebanyak tiga <br /> kali. Pengamatan karakter morfologi dilakukan secara kuantitatif dan <br /> kualitatif pada umur 2, 3, dan 4 BST. Hasil penelitian menunjukkan aksesi <br /> berpengaruh terhadap radiosensitivitas. Aksesi HALINA 1 lebih sensitif <br /> dibandingkan   dengan   ZIOF 0048   ditunjukkan   dengan   nilai   LD50 <br /> HALINA 1 (11,07 Gy) lebih rendah dari LD50 ZIOF 0048 (13,43 Gy). <br /> Interaksi aksesi pada dosis 5 dan 10 Gy memiliki nilai terbaik pada  tinggi <br /> tanaman, ukuran daun serta jumlah anakan. Dosis mempengaruhi tinggi <br /> tanaman, ukuran daun, diameter dan panjang batang, serta jumlah daun. <br /> Pertumbuhan tanaman semakin meningkat dengan bertambahnya umur <br /> tanaman. Namun, semakin tinggi dosis iradiasi, pertumbuhan tanaman <br /> semakin melambat.  Pertumbuhan tanaman terbaik diperoleh pada dosis 5 <br /> Gy, dan tidak berbeda dengan kontrol. Bobot dan ukuran rimpang terbaik <br /> (964 g/rumpun) diperoleh dari HALINA 1 pada dosis 10 Gy, dan berbeda <br /> dengan kontrol (454 g/rumpun), serta perlakuan lainnya.</p><p>Kata kunci:  Zingiber officinale var. amarum, iradiasi sinar gamma (<sup>60</sup>Co), keragaman genetik, mutasi, karakter morfologi.</p><p> </p><p>The Effect of Gamma Irradiation (<sup>60</sup>Co) on the Growth and Production of Small White Ginger (Zingiber officinale var. amarum)</p><p>ABSTRACT</p><p>Genetic variability of ginger germplasm was low, therefore it was <br /> necessary to broaden genetic variability using gamma irradiation. This <br /> research aimed to see the effect of irradiation on the growth of small white <br /> ginger. This study was conducted in the Plant Breeding Laboratory, <br /> Indonesian Spice and Medicinal Crops Research Institute and the Cicurug <br /> Experimental Garden Sukabumi from June 2010 to May 2011. Irradiation <br /> was undertaken in P3TIR, BATAN, Jakarta. The experiment was arranged <br /> in a split plot design with the main plot was two accessions HALINA 1 <br /> and ZIOF 00 48, and the sub plots was 11 irradiation doses (0-50 Gy) with 5 Gy interval.  The experiment was replicated three times.  Parameters <br /> observed were quantitative and qualitative characters at 2,3 and 4 months <br /> after   planting (MAPs).   Results   showed   that   accession   influenced <br /> radiosensitivity. Accession HALINA 1 was more radiosensitive than ZIOF <br /> 0048, indicated by LD50 of HALINA 1 was 11.0693 Gy, lower than ZIOF <br /> 0048 13.4254 Gy. Interaction between accessions at 5 and 10 Gy showed <br />the highest plant height, leaf size, and number of shoots. Irradiation dose affected plant height, leaf size, stem diameter and length, and number of leaf. The plant growth increased in conjunction with plant age, but the <br /> higher irradiation dose, the growth of plants slowed down. The best plant <br /> growth was obtained from 5 Gy, not different from control. The highest <br /> rhizome weight (964 g/plant) was obtained from HALINA 1 treated with 10 Gy, differ from control (545 g/plant) and the other treatments.</p><p>Keywords:  Zingiber officinale var. amarum, gamma irradiation (<sup>60</sup>Co), genetic variability, mutation, morphological characters.</p>

scholarly journals Increasing genetic variability in black oats using gamma irradiation

2014 ◽  
Vol 13 (4) ◽  
pp. 10332-10340 ◽  
Author(s):  
G. Silveira ◽  
E. Moliterno ◽  
G. Ribeiro ◽  
P.M.A. Costa ◽  
L.G. Woyann ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Gamma Irradiation

Role of genetic variability in the renin-angiotensin system in diabetic and nondiabetic renal disease

2001 ◽  
Vol 21 (6) ◽  
pp. 580-592 ◽  
Author(s):  
Arnold Boonstra ◽  
Dick de Zeeuw ◽  
Paul E. de Jong ◽  
Gerjan Navis
Keyword(s):  
Genetic Variability ◽  
Renal Disease ◽  
Renin Angiotensin System ◽  
Angiotensin System ◽  
Renin Angiotensin
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