scholarly journals The Oxidative Derivatization Method For The Spectrofluorimetric Determination Of Periciazine In Pharmaceutical Preparations

2020 ◽  
Vol 15 (1) ◽  
pp. 21-26
Author(s):  
M.Ye. Blazheyevskiy ◽  
Yu.V. Scrypynets ◽  
A.V. Yegorova ◽  
V.P. Antonovich
Keyword(s):  
Hydrochloric Acid ◽  
Acid Solution ◽  
Quantitative Determination ◽  
Pharmaceutical Preparations ◽  
Oral Solution ◽  
Calibration Plot ◽  
Highly Sensitive ◽  
Spectrofluorimetric Determination ◽  
Derivatizing Agent

The oxidative derivatization method for the indirect spectrofluorimetric determination of Periciazine has been presented. Potassium hydrogenperoxymonosulfate (Oxone ®) is proposed as a derivatizing agent for Periciazine, yielding the strongly fluorescent Periciazine sulfoxide. A highly sensitive, simple and rapid method for determination of the Periciazine by fluorescence of its oxidation product with Oxone ® solution in 0.02 M hydrochloric acid solution (λex = 364 nm; λem = 444 nm) has been developed. The calibration plot is linear in concentration range of 0.05 – 4.00 µg mL -1 . LOQ (10S) is 0.05 µg mL -1 . The possibility of quantitative determination of Periciazine in pharmaceutical preparations (Neuleptil ®, 10 mg capsules and Neuleptil ®, a 30 mL 4 % oral (solution) drops) has been shown RSD < 2.2 % (δ < RSD).

scholarly journals A New Oxidative Derivatization Method For The Indirect Spectrofluorimetric Determination Of Prochlorperazine Maleate In Pharmaceutical Preparations

2019 ◽  
Vol 14 (3) ◽  
pp. 140-145
Author(s):  
M.Ye. Blazheyevskiy ◽  
Yu.V. Skrypynets ◽  
A.V. Yegorova ◽  
V.P. Antonovich
Keyword(s):  
Sulfuric Acid ◽  
Acid Solution ◽  
Quantitative Determination ◽  
Calibration Curve ◽  
Pharmaceutical Preparations ◽  
Limit Of Quantification ◽  
Highly Sensitive ◽  
Spectrofluorimetric Determination ◽  
Derivatizing Agent

A new oxidative derivatization method for the indirect spectrofluorimetric determination of Prochlorperazine maleate has been presented. Potassium hydrogenperoxomonosulphate is proposed as a derivatizing agent for Prochlorperazine, yielding the strongly fluorescent sulfoxide. This reaction product was successfully employed for the spectrofluorimetric determination of the Prochlorperazine maleate. A highly sensitive, simple and rapid method has been developed for determining prochlorperazine maleate in tablets by fluorescence of its oxidation product with Oxone solution in 0.01 M sulfuric acid solution (λex = 340 nm; λem = 380 nm). The calibration curve is linear in its concentration range of 0.8–10.0 µg/ml. Limit of quantification (LOQ = 10S) is 0.8 µg/ml. The possibility of quantitative determination of Prochlorperazine maleate in Vertinex® tablets 5 mg has been shown, RSD <2.3% (δ <RSD).

scholarly journals Highly Sensitive Spectrofluorimetric Determination of Ephedrine Hydrochloride in Pharmaceutical Preparations

2006 ◽  
Vol 89 (5) ◽  
pp. 1263-1267 ◽  
Author(s):  
Sevgi Tatar Ulu
Keyword(s):  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Official Method ◽  
Good Precision ◽  
Experimental Conditions ◽  
Ephedrine Hydrochloride ◽  
Highly Sensitive ◽  
Relative Standard ◽  
Spectrofluorimetric Determination

Abstract A sensitive and specific spectrofluorimetry method was developed and validated for the quantification of ephedrine (EP) in pharmaceutical preparations. The method is based on the fluorescent enhancing reaction of EP with 7-chloro-4-nitrobenzofurazan (NBD-CI; derivatization reagent), in borate buffer of pH 9 to yield a yellow, fluorescent product. Under these experimental conditions, the derivatized product of EP had excitation and emission wavelength maxima at 458 and 516 nm, respectively. The linear range of this method was 202500 ng/mL. The detection limit was 7.3 ng/mL EP. Intra- and interday precisions of the assay at 3 concentrations within this range were 0.0371.77%. The low relative standard deviation values indicate good precision, and high recovery values indicate excellent accuracy of the method. The proposed method was applied to the determination of the examined drugs in pharmaceutical formulations, and the results indicate that the method is equally as accurate, precise, and reproducible as the official method.

Simultaneous Determination of Methocarbamol and Paracetamol in the Presence of Three Related Substances by Ultra Performance Liquid Chromatography

2019 ◽  
Vol 15 (5) ◽  
pp. 505-510
Author(s):  
Yanjuan Zheng ◽  
Qiushi Peng ◽  
Rui Dong ◽  
Tingyu Chen ◽  
Yi Bao ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Pharmaceutical Preparations ◽  
Gradient Elution ◽  
Ultra Performance Liquid Chromatography ◽  
Related Substances ◽  
Bulk Powder ◽  
Highly Sensitive ◽  
Optimal Protocol ◽  
Acquity Uplc

Introduction: A rapid, and accurate Ultra Performance Liquid Chromatography (UPLC) method was developed to simultaneously analyze Methocarbamol, Paracetamol and the related substances Materials and Methods: Waters ACQUITY UPLC® BEH Phenyl C18 column was used in conjunction with UV detection at 225nm. Gradient elution with 0.05M, pH 6 phosphate buffer and acetonitrile flow at 0.3mL /min rate were used to separate the substances. The retention times for 4-Aminopheno, Paracetamol, Guaifenesin, Methocarbamol, and 4-Chloroacetanilide were 1.319 minute, 2.224 minute, 4.467 minute, 4.769 minute and 5.433 minute respectively. The concentration was linear in the range of 2-100 µg/ml for Methocarbamol, and 1-100 µg/mL for Paracetamol. The percentage recoveries were between 99.28±1.23% to 100.57±0.99% for Methocarbamol, and between 99.08±1.23% to 101.23±1.39% for Paracetamol. Results and Discussion: The validated optimal protocol is robust and accurate for simultaneous analysis of Methocarbamol, Paracetamol and the related substances, applicable for bulk powder as well as pharmaceutical formulation. Conclusion: In this paper, a highly sensitive, accurate, and precise UPLC method with UV-Vis detection was developed and validated for quality control of MET and PAR in bulk as well as in pharmaceutical preparations.

scholarly journals Highly Sensitive Spectrofluorimetric Determination of Doxycycline Using Doxycycline-Europiumion-Sodium Dodecyl Sulfonate System

2013 ◽  
Vol 25 (6) ◽  
pp. 3321-3324 ◽  
Author(s):  
Baoxiu Jia ◽  
Ping Zhang ◽  
Renliang Wang ◽  
Yuqin Li ◽  
Caihong Liu ◽  
...  
Keyword(s):  
Sodium Dodecyl ◽  
Sodium Dodecyl Sulfonate ◽  
Highly Sensitive ◽  
Spectrofluorimetric Determination

scholarly journals Difference spectrophotometric method for the determination of Fluphenazine hydrochloride in tablets using peroxomonosulfate

2021 ◽  
Vol 9 (2) ◽  
pp. 64-71
Author(s):  
Mykola Blazheyevskiy ◽  
◽  
Valeriy Moroz ◽  
Olena Mozgova ◽  
◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Present Method ◽  
Molar Absorptivity ◽  
Limit Of Quantification ◽  
Derivatizing Agent ◽  
Sensitive Method

The oxidative derivatization method using potassium hydrogenperoxomonosulfate for the indirect spectrophotometric determination of Fluphenazine hydrochloride is presented. Potassium hydrogenperoxomonosulfate is introduced as a derivatizing agent for Fluphenazine hydrochloride, yielding the sulfoxide. This reaction product was successfully used for the spectrophotometric determination of the Fluphenazine hydrochloride. The UV spectroscopic detection of the sulfoxide proved to be a more robust and sensitive method. The elaborated method allowed the determination of Fluphenazine hydrochloride in the concentration range of 0.2-30 µg mL-1. The molar absorptivity at 349 nm is 5.6×103 (dm3cm-1mol-1). The limit of quantification, LOQ (10S) is 0.24 µg/mL. A new spectrophotometric technique was developed and the possibility of quantitative determination of Fluphenazine hydrochloride in tablets 5.0 mg was demonstrated. The present method is precise, accurate and excipients did not interfere. RSD for Fluphenazine Hydrochloride 5.0 mg tablets was 1.37 %.

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