Genotype diversity assessment of common snowdrop (Galanthus nivalis L.) based on chlorophyll and carotenoids partitioning among different plant organs

2021 ◽  
Vol 86 (3) ◽  
pp. 252-259
Author(s):  
G. Osterc ◽  
◽  
B. Ravnjak ◽  
M. Hudina ◽  
J. Bavcon ◽  
...  
Keyword(s):  
Plant Organs ◽  
Diversity Assessment ◽  
Genotype Diversity ◽  
Galanthus Nivalis

Differential expression of indole alkaloid pathway genes across Catharanthus roseus plant organs

2020 ◽  
Vol 52 (4) ◽  
Author(s):  
Ahmed M. Ramadan ◽  
Muna A. Abdulgader ◽  
Thana Khan ◽  
Nour O. Gadalla ◽  
Ahmed Bahieldin
Keyword(s):  
Differential Expression ◽  
Catharanthus Roseus ◽  
Indole Alkaloid ◽  
Plant Organs

AFLP-derived, Codominant Markers for Locus-specific Applications

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 514e-514
Author(s):  
James M. Bradeen ◽  
Philipp W. Simon
Keyword(s):  
Linkage Mapping ◽  
Large Scale ◽  
Pcr Primers ◽  
Inverse Pcr ◽  
Sequence Information ◽  
Pcr Assay ◽  
Specific Primers ◽  
Simultaneous Evaluation ◽  
Feral Populations ◽  
Diversity Assessment

The amplified fragment length polymorphism (AFLP) is a powerful marker, allowing rapid and simultaneous evaluation of multiple potentially polymorphic sites. Although well-adapted to linkage mapping and diversity assessment, AFLPs are primarily dominant in nature. Dominance, relatively high cost, and technological difficulty limit use of AFLPs for marker-aided selection and other locus-specific applications. In carrot the Y2 locus conditions carotene accumulation in the root xylem. We identified AFLP fragments linked to the dominant Y2 allele and pursued conversion of those fragments to codominant, PCR-based forms useful for locus-specific applications. The short length of AFLPs (≈60 to 500 bp) precludes development of longer, more specific primers as in SCAR development. Instead, using sequence information from cloned AFLP fragments for primer design, regions outside of the original fragment were amplified by inverse PCR or ligation-mediated PCR, cloned, and sequenced. Differences in sequences associated with Y2 vs. y2 allowed development of simple PCR assays differentiating those alleles. PCR primers flanking an insertion associated with the recessive allele amplified differently sized products for the two Y2 alleles in one assay. This assay is rapid, technologically simple (requiring no radioactivity and little advanced training or equipment), reliable, inexpensive, and codominant. Our PCR assay has a variety of large scale, locus-specific applications including genotyping diverse carrot cultivars and wild and feral populations. Efforts are underway to improve upon conversion technology and to more extensively test the techniques we have developed.

Distribution of Nutrients in Snap Bean Plant Organs as Affected by N, P and K Fertilizer Levels

2017 ◽  
Vol 8 (6) ◽  
pp. 209-214
Author(s):  
A. El-Ghamry ◽  
G. Baddoor ◽  
Dina Ghazi ◽  
M. EL Hassanin
Keyword(s):  
Bean Plant ◽  
Snap Bean ◽  
Plant Organs ◽  
K Fertilizer

scholarly journals Phylogeny reveals non‐random medicinal plant organs selection by local people in Benin

2021 ◽  
Author(s):  
Orou G. Gaoue ◽  
Kowiyou Yessoufou ◽  
Ledile Mankga ◽  
Fifanou Vodouhe
Keyword(s):  
Medicinal Plant ◽  
Local People ◽  
Plant Organs

Cross-species microsatellite amplification in Vasconcellea and related genera and their use in germplasm classification

Genome ◽  
2006 ◽  
Vol 49 (7) ◽  
pp. 786-798 ◽  
Author(s):  
T Kyndt ◽  
B Van Droogenbroeck ◽  
A Haegeman ◽  
I Roldán-Ruiz ◽  
G Gheysen
Keyword(s):  
Population Genetics ◽  
Chloroplast Microsatellites ◽  
Microsatellite Data ◽  
Chloroplast Microsatellite ◽  
Diversity Assessment ◽  
Ssr Primers ◽  
Polymorphism Rate ◽  
Nuclear Ssr ◽  
Simple Sequence ◽  
Future Population

To generate inexpensive and efficient DNA markers for addressing a number of population genetics problems and identification of wild hybrids in Vasconcellea, we have evaluated the use of simple sequence repeat (SSR) primers previously developed for other species. A set of 103 Vasconcellea accessions and some individuals of the related genera Carica and Jacaratia were analyzed with 10 primer pairs directing amplification of chloroplast microsatellites in Nicotiana tabacum and 9 nuclear SSR primer pairs recently identified in Vasconcellea × heilbornii. Heterologous amplification of chloroplast SSRs was successful for 8 of the 10 loci, of which 6 showed polymorphism. Seven of the 9 nuclear SSR primer pairs were useful in Vasconcellea and often also in Jacaratia and Carica, all revealing polymorphism. Exclusive haplotypes for each described taxon were identified based on chloroplast microsatellite data. Clustering based on separate nuclear and chloroplast data resulted in a clear grouping per taxon, but only low resolution was obtained above species level. The codominancy of nuclear SSRs and the general high polymorphism rate of SSR markers will make them more useful in future population genetics studies and diversity assessment in conservation programs.Key words: Carica, Jacaratia, Vasconcellea, simple sequence repeats, cross-species amplification, classification, interspecific hybrids.

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