Anti-allergic Effect of Eckolona cava Ethyl Acetate Fraction of on IgE/BSA-stimulated Bone Marrow-derived Cultured Mast Cells

2018 ◽  
Vol 23 (4) ◽  
pp. 277-284
Author(s):  
Eui Jeong Han ◽  
Hyun Soo Kim ◽  
Eun Ji Shin ◽  
Min Ju Kim ◽  
Hee-jin Han ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Allergic Effect

scholarly journals The Protective Effects of the Ethyl Acetate Part of Er MiaoSan on Adjuvant Arthritis Rats by Regulating the Function of Bone Marrow-Derived Dendritic Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Jiemin Ding ◽  
Min Liu ◽  
Zihua Xuan ◽  
Meng li Liu ◽  
Ning Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Proliferation ◽  
Dendritic Cells ◽  
Ethyl Acetate ◽  
B Cell ◽  
Adjuvant Arthritis ◽  
Ethyl Acetate Fraction ◽  
Costimulatory Molecules ◽  
Inflammatory Factor ◽  
Protective Effects

Aims. The aim of this study was to evaluate the protective effects of Er Miao San (EMS) and the regulative function of bone marrow-derived dendritic cells (BMDCs) on adjuvant arthritis (AA) in rats. Methods. The ethyl acetate part of EMS (3 g/kg, 1.5 g/kg, and 0.75 g/kg) was orally administered from day 15 after immunization to day 29. The polyarthritis index and paw swelling were measured, the ankle joint pathological changes were observed using hematoxylin-eosin (HE) staining, and the spleen and thymus index were determined. Moreover, T and B cell proliferation were determined using the CCK-8 assay. The expression of BMDC surface costimulatory molecules and inflammatory factors were determined using flow cytometry and ELISA kits, respectively. Results. Compared with the AA model rats, the ethyl acetate fraction of EMS obviously reduced paw swelling (from 1.0 to 0.7) and the polyarthritis index (from 12 to 9) P < 0.01 and improved the severity of histopathology P < 0.01 . The treatment using ethyl acetate fraction of EMS significantly reduced the spleen and thymus index P < 0.01 and inhibited T and B cell proliferation P < 0.01 . Moreover, EMS significantly modulated the expression of surface costimulatory molecules in BMDCs, including CD40, CD80, CD86, and major histocompatibility complex class II (MHC-II) P < 0.01 . The results also showed that the ethyl acetate part of EMS significant inhibited the levels of proinflammatory cytokines interleukin- (IL-) 23 tumor necrosis factor- (TNF-) α and inflammatory factor prostaglandin (PG) E2 in the supernatant of BMDCs. However, the level of anti-inflammatory cytokine IL-10 was significantly increased P < 0.01 . Conclusion. These results suggest that the ethyl acetate part of EMS has better protective effects on AA rats, probably by regulating the function of BMDCs and modulating the balance of cytokines.

scholarly journals Effect of the Extract and Constituents From Hancornia speciosa Fruits in Osteoclasts

2019 ◽  
Vol 6 (01) ◽  
pp. e7-e14 ◽  
Author(s):  
Micena Alves e Silva ◽  
Cinthia Pacheco ◽  
Mila Madeira ◽  
Adriana Saraiva ◽  
Elisângela de Freitas ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Ethyl Acetate ◽  
Bone Marrow Cell ◽  
Cell Cultures ◽  
Marrow Cell ◽  
Ethanolic Extract ◽  
Quinic Acid ◽  
Ethyl Acetate Fraction ◽  
Raw 264.7 ◽  
Hancornia Speciosa

Abstract Hancornia speciosa is a medicinal species traditionally used in Brazilian folk medicine to treat a variety of conditions. Compounds isolated from the leaves, bark, and trunk of this plant have shown therapeutic properties, but only recently have the fruits of H. speciosa been explored for potential pharmacological applications. The present study investigated the effects of an ethanolic extract from the fruits, fractions, and compounds thereof in bone resorbing cells. Primary osteoclast cultures from bone marrow cells and osteoclasts derived from a monocyte/macrophage cell line, RAW 264.7, were incubated with different concentrations of the ethanolic extract, ethyl acetate fraction, water fraction, quinic acid, and L-(+)-bornesitol. In RAW 264.7 cell cultures, quinic acid significantly reduced osteoclast formation. In bone marrow cell-derived osteoclasts, the ethyl acetate fraction induced a decrease in the number of osteoclasts, promoting a remarkable reduction in the mean area of those cells and in their resorption activity. The compounds quinic acid and bornesitol also affected bone marrow cell-derived osteoclasts. In both cell cultures, the substances tested did not affect cell viability/proliferation. In conclusion, components extracted from H. speciosa fruit affected the cells responsible for bone resorption, making them promising tools for interference in osteoclastogenesis.

Anti-Inflammatory and Anti-Allergic Effect of Agaricus blazei Extract in Bone Marrow-Derived Mast Cells

2012 ◽  
Vol 40 (05) ◽  
pp. 1073-1084 ◽  
Author(s):  
Hyuk-Hwan Song ◽  
Hee-Sung Chae ◽  
Sei-Ryang Oh ◽  
Hyeong-Kyu Lee ◽  
Young-Won Chin
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Mouse Bone Marrow ◽  
Agaricus Blazei ◽  
Soluble Extract ◽  
Anti Inflammatory ◽  
Allergic Effect

In this study, the anti-inflammatory and anti-allergic effects of the chloroform-soluble extract of Agaricus blazei in mouse bone marrow-derived mast cells (BMMCs) were investigated. The chloroform-soluble extract inhibited IL-6 production in PMA plus A23187-stimulated BMMCs, and down-regulated the phosphorylation of Akt. In addition, this extract demonstrated inhibition of the degranulation of β-hexosaminidase and the production of IL-6, prostaglandin D2 and leukotriene C4 in PMA plus A23187-induced BMMCs. In conclusion, the chloroform-soluble extract of Agaricus blazei exerted anti-inflammatory and anti-allergic activities mediated by influencing IL-6, prostaglandin D2, leukotriene C4, and the phosphorylation of Akt.

Gomisin N has anti-allergic effect and inhibits inflammatory cytokine expression in mouse bone marrow-derived mast cells

2011 ◽  
Vol 33 (4) ◽  
pp. 709-713 ◽  
Author(s):  
Hee-Sung Chae ◽  
Ok-Hwa Kang ◽  
You-Chang Oh ◽  
Jang-Gi Choi ◽  
Joon-Ho Keum ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Inflammatory Cytokine ◽  
Cytokine Expression ◽  
Mouse Bone Marrow ◽  
Gomisin N ◽  
Allergic Effect

Anti-allergic effect of a chloroform-soluble extract ofCinnamomum cambodianumin bone marrow-derived mast cells

2012 ◽  
Vol 34 (4) ◽  
pp. 639-644 ◽  
Author(s):  
Hee-Sung Chae ◽  
Piseth Khiev ◽  
Hyeong-Kyu Lee ◽  
Sei-Ryang Oh ◽  
Young-Won Chin
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Soluble Extract ◽  
Allergic Effect

scholarly journals Ethno Botanical Survey, Anti-leukemia and Anticlastogenic Potential of Medicinal Plants used for Treatment of Leukemia in Oyo State Nigeria

2020 ◽  
pp. 31-51
Author(s):  
Adewale, Adetutu ◽  
Olusoji Abiodun, Owoade ◽  
Olanike Alabi ◽  
Fatima Aluko Abubakar ◽  
Olubunmi Simeon Oyekunle
Keyword(s):  
Bone Marrow ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Hematological Parameters ◽  
Alternative Therapy ◽  
Ethyl Acetate Fraction ◽  
Traditional Healers ◽  
Calotropis Procera ◽  
Ethnobotanical Survey ◽  
Piper Guineense

Ethnopharmacological Relevance: Leukemia, a cancer of the blood and bone marrow is responsible for about 222.000 deaths annually. The side effects of chemotherapy in leukemia treatment have necessitated the search for natural products especially medicinal plants as alternative therapy. Aim: This study surveyed common plants used for treating leukemia in Oyo state, Nigeria and assessed the anti-leukemic and anti-clastogenic activities of fractions of Nymphaea lotus. Methods: Semi-structured questionnaire (1000) was used to collect the ethnobotanical data among the traditional healers. Leukemia was induced in albino mice with 400 mg/kg body weight of benzene intraperitoneally. Aqueous extracts (120mg/kg/bw), fractions and 5-florouracil (25mg) was administered to mice of different groups. The anti-leukemic, anti-clastogenic, antioxidant, hepatoprotective activities and hematological parameters were determined. N. lotus was subjected to gas-chromatography mass spectroscopy analysis. Results: The ethnobotanical survey recorded 89 plant species with their local names and parts used in the traditional therapeutic preparations. Seven plants (Pistiastratiotes (2.5%), Nymphaea lotus (1.4%), Piper guineense (1.7%), Securinega virosa (2.5%), Calotropis procera (3.4%), Morinda lucida (2.5%) and Xylopia aethiopica (4.5%) with the highest frequency of citation were selected for anti-leukemic and anti-clastogenic screening. The aqueous extract of N. lotus and M. lucida displayed anti-leukemic potential. M. lucida and X. aethiopica improved the hematological parameters. Ethyl acetate fraction of N. lotus significantly (p<0.05) reduced the number of micronucleated polychromatic erythrocyte in the bone marrow and showed anti-leukemic activity. Fractions of N. lotus restored the hematological parameters and exhibited significant (p<0.05) antioxidant activity. Histological observation revealed improvement in the liver general cyto-architecture of mice treated with ethyl acetate and butanol fractions of N. lotus. Some known compounds were identified in ethyl acetate fraction of N. lotus. Conclusion: Most of the species tested had some anti-leukemic effect in mice, which to some extent supports their traditional inclusion in herbal preparations for treatment of leukemia. The study also identified potential anti-leukemic compounds in N. lotus extract. The study also identified potential anti-leukemic compounds in N. lotus extract.

scholarly journals The anti-allergic effect of the ethyl acetate fraction from anEcklonia kuromeextract

2014 ◽  
Vol 26 (2) ◽  
pp. 181-193 ◽  
Author(s):  
Yoshimasa Sugiura ◽  
Koki Nagayama ◽  
Yuichi Kinoshita ◽  
Ryusuke Tanaka ◽  
Teruo Matsushita
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Allergic Effect
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