scholarly journals PHYSIOLOGICAL RESPONSES TO HIGH EXOGENOUS SUCROSE IN TUBER INDUCTION OF POTATO IN VITRO

2016 ◽  
Vol 21 (2) ◽  
pp. 254
Author(s):  
Xueqin HE ◽  
Meilian MENG
Keyword(s):  
Physiological Responses ◽  
Tuber Induction

scholarly journals In vitro Osmotic Stress Memory in Chrisanthemum hybridum: Structural and Physiological Responses

2017 ◽  
Vol 27 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Lidiia Samarina ◽  
Valentina Malyarovskaya ◽  
Yulija Abilfazova ◽  
Natalia Platonova ◽  
Kristina Klemeshova ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Relative Water Content ◽  
Soluble Protein ◽  
Growth Parameters ◽  
Physiological Responses ◽  
Proline Content ◽  
Stress Exposure ◽  
Stress Memory ◽  
Relative Water

Structural and physiological responses of chrysanthemum to repeated osmotic stress were studied. Plants were cultured for 2 weeks (for each stress1 and stress 2) on half MS supplemented with mannitol 100 mM (Treatment I) and 200 mM (Treatment II). First stress inhibited growth parameters stronger than second stress in treatment I. In treatment II both stress events strongly inhibited growth parameters of micro‐shoots. Proline content exceeded control 6 ‐ 8 times after 1st stress, and 2 ‐ 5 times after the 2nd stress in treatments I and II, respectively. Soluble protein was accumulated in leaves during both stress exposures, and 2 ‐ 2.5 times exceeded control after the 2nd stress. Relative water content in both treatments increased after the 2nd stress exposure. In treatment II chlorophyll а and carotenoids contents were 8.78 and 4.62 mg/g comparing to control (4.21 and 2.25 mg/g, respectively) after the 1st stress. But after the 2nd stress there was no difference with control.Plant Tissue Cult. & Biotech. 27(2): 161-169, 2017 (December)

scholarly journals Rheological and Microstructural Features of Plant Culture Media Doped with Biopolymers: Influence on the Growth and Physiological Responses of In Vitro-Grown Shoots of Thymus lotocephalus

2021 ◽  
Vol 2 (2) ◽  
pp. 538-553
Author(s):  
Natacha Coelho ◽  
Alexandra Filipe ◽  
Bruno Medronho ◽  
Solange Magalhães ◽  
Carla Vitorino ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Culture Media ◽  
Average Pore Size ◽  
Physiological Responses ◽  
Gum Arabic ◽  
Shoot Elongation ◽  
Hydroxyethyl Cellulose ◽  
Plant Culture

In vitro culture is an important biotechnological tool in plant research and an appropriate culture media is a key for a successful plant development under in vitro conditions. The use of natural compounds to improve culture media has been growing and biopolymers are interesting alternatives to synthetic compounds due to their low toxicity, biodegradability, renewability, and availability. In the present study, different culture media containing one biopolymer (chitosan, gum arabic) or a biopolymer derivative [hydroxyethyl cellulose (HEC), carboxymethyl cellulose (CMC)], at 100 or 1000 mg L−1, were tested regarding their influence on the growth and physiological responses of Thymus lotocephalus in vitro culture. Cellulose-based biopolymers (HEC and CMC) and gum arabic were used for the first time in plant culture media. The results showed that CMC at 100 mg L−1 significantly improved shoot elongation while chitosan, at the highest concentration, was detrimental to T. lotocephalus. Concerning only the evaluated physiological parameters, all tested biopolymers and biopolymer derivatives are safe to plants as there was no evidence of stress-induced changes on T. lotocephalus. The rheological and microstructural features of the culture media were assessed to understand how the biopolymers and biopolymer derivatives added to the culture medium could influence shoot growth. As expected, all media presented a gel-like behaviour with minor differences in the complex viscosity at the beginning of the culture period. Most media showed increased viscosity overtime. The surface area increased with the addition of biopolymers and biopolymer derivatives to the culture media and the average pore size was considerably lower for CMC at 100 mg L−1. The smaller pores of this medium might be related to a more efficient nutrients and water uptake by T. lotocephalus shoots, leading to a significant improvement in shoot elongation. In short, this study demonstrated that the different types of biopolymers and biopolymer derivatives added to culture medium can modify their microstructure and at the right concentrations, are harmless to T. lotocephalus shoots growing in vitro, and that CMC improves shoot length.

scholarly journals Differential effects of fibroblast growth factor (FGF) 9 and FGF2 on proliferation, differentiation and terminal differentiation of chondrocytic cells in vitro

1999 ◽  
Vol 342 (3) ◽  
pp. 677-682 ◽  
Author(s):  
Nicole B. WEKSLER ◽  
Gregory P. LUNSTRUM ◽  
Eric S. REID ◽  
William A. HORTON
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Physiological Responses ◽  
Terminal Differentiation ◽  
Receptor Activation ◽  
Fibroblast Growth ◽  
Fgf Receptor ◽  
Chondrocytic Differentiation ◽  
Mitotic Inhibitor

Fibroblast growth factor (FGF) 9 was compared with FGF2 in its ability to influence proliferation, differentiation, terminal differentiation and apoptosis in a rat calvaria-derived cell line (RCJ 3.1C5.18) that spontaneously undergoes chondrocyte differentiation in vitro. Like FGF2, FGF9 promoted proliferation, but to a lesser extent. In contrast to FGF2, which blocked chondrocytic differentiation, FGF9 had no effect on differentiation but inhibited terminal differentiation. FGF9 also stimulated expression of the mitotic inhibitor p21 to a greater extent than FGF2. Neither ligand influenced apoptosis. The results indicate that FGF9 could account for many of the physiological responses attributed to FGF-receptor activation in the growth plate.

scholarly journals Correction to: NaCl-induced stress: physiological responses of six halophyte species in in vitro and in vivo culture

2019 ◽  
Vol 140 (2) ◽  
pp. 469-471
Author(s):  
Yuping Xiong ◽  
Hanzhi Liang ◽  
Haifeng Yan ◽  
Beiyi Guo ◽  
Meiyun Niu ◽  
...  
Keyword(s):  
Physiological Responses ◽  
Induced Stress ◽  
In Vivo Culture

Growth and physiological responses in chicory towards mercury induced in vitro oxidative stress

2019 ◽  
Vol 24 (2) ◽  
pp. 236-248 ◽  
Author(s):  
Bisma Malik ◽  
Tanveer Bilal Pirzadah ◽  
Inayatullah Tahir ◽  
Reiaz Ul Rehman
Keyword(s):  
Oxidative Stress ◽  
Physiological Responses

Morpho-Physiological Responses of Halophyte Climacoptera crassa to Salinity and Heavy Metal Stresses in In Vitro Condition

2020 ◽  
Vol 131 ◽  
pp. 468-474
Author(s):  
Leila Samiei ◽  
Mahboubeh Davoudi Pahnehkolayi ◽  
Zahra Karimian ◽  
Jafar Nabati
Keyword(s):  
Heavy Metal ◽  
Physiological Responses

Simultaneous measurement of intracellular pH and Ca2+ using the fluorescence of SNARF-1 and fura-2

1991 ◽  
Vol 260 (2) ◽  
pp. C297-C307 ◽  
Author(s):  
R. Martinez-Zaguilan ◽  
G. M. Martinez ◽  
F. Lattanzio ◽  
R. J. Gillies
Keyword(s):  
Intracellular Ph ◽  
Simultaneous Measurement ◽  
Physiological Response ◽  
Second Messengers ◽  
Physiological Responses ◽  
High Time Resolution ◽  
Experimental Conditions ◽  
Fura 2 ◽  
Fluorescence Characteristics

Upon cell stimulation with hormones and other mitogens, a variety of biochemical and physiological responses occur within the first few minutes. Changes in both intracellular pH (pHin) and intracellular Ca2+ concentration ([Ca2+]in) are prominent and play a major role in the signal transduction mechanism leading to the physiological response, i.e., secretion, neurotransmission, proliferation, or differentiation. However, it is not clear whether these ions work independently in the activation pathway leading to a particular physiological response. The fluorescence characteristics of most Ca2+ indicators are pH sensitive, and quantitative estimates of [Ca2+]in cannot be made without knowledge of pHin. Thus it is desirable to have a technique to simultaneously monitor these two ions with relatively high time resolution. Here we have developed experimental conditions that allow us to use optimum emission conditions for a pH fluorescent indicator SNARF-1 and optimum excitation conditions for the Ca2+ indicator fura-2. The fluorescence spectra of these compounds are sufficiently different to allow simultaneous measurement of pH and Ca2+ both in vitro and in situ. We have observed simultaneous changes in both pHin and [Ca2+]in in BALB/c 3T3 cells on treatment with the nonfluorescent Ca2+ ionophore 4-bromo-A23187. This temporal relationship between pHin and Ca2+ gives further credence to the interrelationship between these two second messengers in the expression of physiological responses.

Physiological responses to NaCl stress in three wild species of potato in vitro

2009 ◽  
Vol 32 (1) ◽  
pp. 91-101 ◽  
Author(s):  
Fatemeh Daneshmand ◽  
Mohammad Javad Arvin ◽  
Khosrow Manouchehri Kalantari
Keyword(s):  
Wild Species ◽  
Physiological Responses ◽  
Nacl Stress

Study on Assessment of High Temperature and Humidity in Working Environment on Human Health

2012 ◽  
Vol 610-613 ◽  
pp. 739-742 ◽  
Author(s):  
Yu Ping Sun ◽  
Neng Zhu
Keyword(s):  
High Temperature ◽  
Energy Metabolism ◽  
Human Health ◽  
Physiological Responses ◽  
Working Environment ◽  
Heat Diffusion ◽  
Metabolic Heat ◽  
Temperature And Humidity

In this study, a series of references on human physiology and psychology response to hot and humid environments were analyzed. On basis of the thermo metabolism system, energy metabolism system, cardiovascular system and respiratory system, impact mechanism of high temperature and humidity on human health was presented. The results indicate that the high temperature and humidity in working environment have significant impact on human health. The high temperature and humidity cause the reducing of temperature difference in vitro and in vivo, the difficult of metabolic heat diffusion, the significant increasing of energy metabolism and oxygen consumption, heart failure, hypoxia and other physiological responses.

In Vitro Physiological Responses ofFusarium oxysporumf. sp.niveumto Exogenously Applied Syringic Acid

2009 ◽  
Vol 56 (4) ◽  
pp. 386-387 ◽  
Author(s):  
HONG-SHENG WU ◽  
JIA LUO ◽  
YAN-XIA LIU ◽  
AI-QUN CHEN ◽  
ZHU TANG ◽  
...  
Keyword(s):  
Physiological Responses ◽  
Syringic Acid
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