scholarly journals Structured RNA Markers for Genotyping of Tick-Borne Encephalitis Virus

2018 ◽  
Vol 13 (1) ◽  
pp. 13-37
Author(s):  
V.D. Gusev ◽  
L.A. Miroshnichenko ◽  
T.N. Titkova ◽  
Yu.P. Dzhioev ◽  
I.V. Kozlova ◽  
...  
Keyword(s):  
Clinical Manifestations ◽  
Encephalitis Virus ◽  
Correct Identification ◽  
Disease Treatment ◽  
Pathogenic Potential ◽  
Tick Borne Encephalitis ◽  
Comprehensive Information ◽  
Different Types ◽  
Rna Fragments ◽  
Tick Borne Encephalitis Virus

Tick-borne encephalitis is one of the most dangerous natural focal infections. The causative agent of the disease is tick-borne encephalitis virus (TBEV ) transmitted by ticks . There are three main subtypes of TBEV with different clinical manifestations of the disease, but existence of other subtypes is also possible. Effectiveness of the disease treatment can largely depend on the correct identification of TBEV genotype. Comprehensive information about the genotype is contained in the full coding sequence of TBEV genome. А limited number of genotyping markers can be extracted from it in the form of relatively short structured RNA fragments. In this paper, a rather general approach to the isolation of structured RNA markers for the genotyping of TBEV is formulated. Three types of structures are considered: periodicity, fractal-like constructs and compactly localized combinations of different types of repetitions. The choice of these structures for the purposes of genotyping and their possible role in the formation of the pathogenic potential of the virus is substantiated. The approach was tested on the full coding sequences of TBEV (161 strains). Examples of the most characteristic markers of each of the three types are given.

scholarly journals Tick-borne encephalitis virus – a review of an emerging zoonosis

2009 ◽  
Vol 90 (8) ◽  
pp. 1781-1794 ◽  
Author(s):  
K. L. Mansfield ◽  
N. Johnson ◽  
L. P. Phipps ◽  
J. R. Stephenson ◽  
A. R. Fooks ◽  
...  
Keyword(s):  
Clinical Manifestations ◽  
Northern China ◽  
Encephalitis Virus ◽  
Ixodid Ticks ◽  
Ixodes Persulcatus ◽  
Tick Borne Encephalitis ◽  
Far Eastern Russia ◽  
Tick Borne Encephalitis Virus ◽  
Far Eastern

During the last 30 years, there has been a continued increase in human cases of tick-borne encephalitis (TBE) in Europe, a disease caused by tick-borne encephalitis virus (TBEV). TBEV is endemic in an area ranging from northern China and Japan, through far-eastern Russia to Europe, and is maintained in cycles involving Ixodid ticks (Ixodes ricinus and Ixodes persulcatus) and wild vertebrate hosts. The virus causes a potentially fatal neurological infection, with thousands of cases reported annually throughout Europe. TBE has a significant mortality rate depending upon the strain of virus or may cause long-term neurological/neuropsychiatric sequelae in people affected. In this review, we comprehensively reviewed TBEV, its epidemiology and pathogenesis, the clinical manifestations of TBE, along with vaccination and prevention. We also discuss the factors which may have influenced an apparent increase in the number of reported human cases each year, despite the availability of effective vaccines.

scholarly journals THE DISTRIBUTION OF TICK-BORNE ENCEPHALITIS VIRUS GENOTYPES IN DIFFERENT TYPES OF LANDSCAPES OF EASTERN SIBERIA

2017 ◽  
Vol 2 (5) ◽  
pp. 69-75
Author(s):  
Марина Верхозина ◽  
Marina Verkhozina ◽  
Ирина Козлова ◽  
Irina Kozlova ◽  
Елена Дорощенко ◽  
...  
Keyword(s):  
Encephalitis Virus ◽  
Eastern Siberia ◽  
Tick Borne Encephalitis ◽  
Different Types ◽  
Virus Genotypes ◽  
Tick Borne Encephalitis Virus

scholarly journals A trans-Complementing Recombination Trap Demonstrates a Low Propensity of Flaviviruses for Intermolecular Recombination

2009 ◽  
Vol 84 (1) ◽  
pp. 599-611 ◽  
Author(s):  
Christian Taucher ◽  
Angelika Berger ◽  
Christian W. Mandl
Keyword(s):  
Experimental System ◽  
Encephalitis Virus ◽  
Wild Type Virus ◽  
Wild Type ◽  
Pathogenic Potential ◽  
Tick Borne Encephalitis ◽  
Infectious Clones ◽  
Growth Properties ◽  
Spiking Experiment ◽  
Tick Borne Encephalitis Virus

ABSTRACT Intermolecular recombination between the genomes of closely related RNA viruses can result in the emergence of novel strains with altered pathogenic potential and antigenicity. Although recombination between flavivirus genomes has never been demonstrated experimentally, the potential risk of generating undesirable recombinants has nevertheless been a matter of concern and controversy with respect to the development of live flavivirus vaccines. As an experimental system for investigating the ability of flavivirus genomes to recombine, we developed a “recombination trap,” which was designed to allow the products of rare recombination events to be selected and amplified. To do this, we established reciprocal packaging systems consisting of pairs of self-replicating subgenomic RNAs (replicons) derived from tick-borne encephalitis virus (TBEV), West Nile virus (WNV), and Japanese encephalitis virus (JEV) that could complement each other in trans and thus be propagated together in cell culture over multiple passages. Any infectious viruses with intact, full-length genomes that were generated by recombination of the two replicons would be selected and enriched by end point dilution passage, as was demonstrated in a spiking experiment in which a small amount of wild-type virus was mixed with the packaged replicons. Using the recombination trap and the JEV system, we detected two aberrant recombination events, both of which yielded unnatural genomes containing duplications. Infectious clones of both of these genomes yielded viruses with impaired growth properties. Despite the fact that the replicon pairs shared approximately 600 nucleotides of identical sequence where a precise homologous crossover event would have yielded a wild-type genome, this was not observed in any of these systems, and the TBEV and WNV systems did not yield any viable recombinant genomes at all. Our results show that intergenomic recombination can occur in the structural region of flaviviruses but that its frequency appears to be very low and that therefore it probably does not represent a major risk in the use of live, attenuated flavivirus vaccines.

TBE in South Korea

2019 ◽  
Author(s):  
Joon Young Song
Keyword(s):  
South Korea ◽  
Human Case ◽  
Encephalitis Virus ◽  
Surveillance Studies ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

Although no human case of tick-borne encephalitis (TBE) has been documented in South Korea to date, surveillance studies have been conducted to evaluate the prevalence of tick-borne encephalitis virus (TBEV) in wild ticks.

TBE in Slovakia

2019 ◽  
Author(s):  
Jana Kerlik
Keyword(s):  
Encephalitis Virus ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

The former Czechoslovak Republic was one of the first countries in Europe where the tick-borne encephalitis virus (TBEV) was identified.

Knowledge and vaccination practices among family physicians in northeastern France regarding tick-borne encephalitis virus.

2021 ◽  
pp. 101774
Author(s):  
Marie Dollat ◽  
Anne-Pauline Bellanger ◽  
Laurence Millon ◽  
Catherine Chirouze ◽  
Quentin Lepiller ◽  
...  
Keyword(s):  
Family Physicians ◽  
Encephalitis Virus ◽  
Tick Borne Encephalitis ◽  
Northeastern France ◽  
Tick Borne Encephalitis Virus
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