scholarly journals Evaluation of high sensitivity C-reactive protein assay in cerebrospinal fluid on the Dimension RxL analyzer

2012 ◽  
Vol 2 (1) ◽  
pp. 13-16
Author(s):  
Jozo Ćorić ◽  
Aleksandra Pašić ◽  
Mirsad Panjeta ◽  
Jasminka Mujić
Keyword(s):  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
High Sensitivity ◽  
Acute Bacterial Meningitis ◽  
Control Group ◽  
C Reactive Protein ◽  
Protein Assay ◽  
Reactive Protein ◽  
The Mean ◽  
Low Sensitivity

Introduction: Low sensitivity and specificity in traditional laboratory tests became insufficient for accurate diagnostics and initiation of proper treatment of patients infected with bacterial meningitis. High sensitivity C reactive protein (hsCRP) may be an appropriate supplement for rapid diagnosis of bacterial meningitis. The subject of our investigation was the determination of C- reactive protein in cerebrospinal fluid (CSF) duringacute bacterial meningitis.Methods: HsCRP was analysed by a sensitive immunoturbidimetric assay using the Dimension RxL analyser (Siemens). Cerebrospinal fluid concentrations of C-reactive protein have been measured in 20 patients(age range,1 to 50 years) presenting with acute bacterial meningitis and also in a non-infected, non-inflamed control group (n=25).Results: The accuracy and precision of the method proved to be satisfactory. Repeatability of serial sampling for hsCRP described by coefficient of variation were CV=2.1-4.5%. This assay hsCRP in cerebrospinal fluid demonstrates adequate performance characteristics for routine clinical use. Elevated levels of CRP were found in 95% patients with bacterial meningitis. The mean CRP value in 25 uninfected control group was 0.25 mg/L (range 0.10-0.55). The mean CRP for patients with bacterial meningitis was 21.4 mg/L (range 0.40-100).Conclusions: A sensitive assay for CRP in CSF would be an useful adjunct to conventional investigation of acute infective meningitis.

The Use of Cytokine and C-Reactive Protein Measurements in Cerebrospinal Fluid during Acute Infective Meningitis

1994 ◽  
Vol 31 (3) ◽  
pp. 255-261 ◽  
Author(s):  
M Stearman ◽  
H J Southgate
Keyword(s):  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
White Cell Count ◽  
Correct Diagnosis ◽  
Viral Meningitis ◽  
Acute Bacterial Meningitis ◽  
Control Group ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Factor Α

Cerebrospinal fluid (CSF) concentrations of C-reactive protein (CRP), tumour necrosis factor α (TNF), interleukin-6 (IL-6), total protein (TP) and white cell count with differential (WCC) have been measured in 24 patients presenting with acute bacterial or viral meningitis and also in a non-infected, non-inflammed control group ( n = 24). In acute viral meningitis, CRP levels were not raised when compared to controls and there was a discordance between high levels of the primary inflammatory mediators (IL-6 and TNF) and the low measured CRP levels. CRP levels were raised in cases of bacterial meningitis. A concentration of 100 ng/mL CRP had a sensitivity of 87% for bacterial meningitis. TNF concentrations in the CSF were significantly raised in cases of acute bacterial meningitis ( P < 0·001). Smaller but variable elevations were seen in the patients with acute viral meningitis. One patient, who succumbed to bacterial infection, showed low CSF levels of CRP, TNF and WCC but an elevated IL-6 concentration. Another, presenting with low CSF WCC, had raised concentrations of CRP, TNF and IL-6 which pointed to the correct diagnosis of acute bacterial meningitis. The development of methods yielding rapid analysis for these cytokines together with a sensitive assay for CRP in CSF would be a useful adjunct to conventional investigation.

scholarly journals High Sensitivity C-reactive Protein in Patients with Coronary Artery in-stent Restenosis: A Case-control Study

2021 ◽  
Vol 15 (1) ◽  
pp. 29-37
Author(s):  
Hussein M. Ismail ◽  
Ahmed O. Abaza ◽  
Gamela M. Nasr ◽  
Hesham Hegazy
Keyword(s):  
Coronary Artery ◽  
Case Control Study ◽  
High Sensitivity ◽  
Control Group ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Stent Restenosis ◽  
The Mean ◽  
Serial Measurements ◽  
Control Study

Background: Inflammation plays a pivotal role in the pathogenesis of In-Stent Restenosis (ISR). High sensitivity C-reactive protein (hsCRP) is positively associated with major cardiovascular events. Aim: We aimed to investigate the hsCRP inflammatory response to Percutaneous Coronary Intervention (PCI) in Coronary Artery Disease (CAD) patients with coronary ISR vs. patients without ISR. Methods: This case-control study included 80 CAD patients previously treated with drug-eluting stent (DES) implantation. Patients had Coronary Angiography (CAG) because of chest pain or equivalent symptoms and were subdivided into 2 groups. Group A (n=40) included CAD patients with ISR. Group B (n=40) included age and gender-matched controls with CAD but without ISR. Serum hsCRP levels were obtained before PCI (baseline) and 8, 16, 24 h post-PCI. Results: At baseline (before intervention/CAG), the hsCRP level was increased in the ISR group compared with the No-ISR group (p=0.007). There were 36 (90%) patients in the ISR group who had a high hsCRP (>3 mg/L) compared with 25 (62.5%) patients in the No-ISR group. Also, there was a significant relationship between high hsCRP and the ISR. Patients with ISR had higher frequencies and percentages of elevated CRP than the no-ISR control group. This difference was maintained for all measurements, baseline, after 8, 16, and 24 h (p<0.05). Repeated measures analysis of variance (ANOVA) in the ISR group revealed that mean hsCRP differed significantly between serial measurements (p<0.001). In contrast, in the control group, the mean hsCRP did not differ significantly between the serial measurements (p=0.65). Most of our patients (n=66, 82.5%) had 1-vessel CAD disease, and the left anterior descending (LAD) coronary artery was significantly affected in 46 patients (57.5%). Management of restenosis was accomplished mainly by stenting by DES in 29 patients (72.5%). Conclusion: Patients with ISR had substantially higher pre- and post-PCI hsCRP levels than the no-ISR controls. This difference was maintained up to 24h post-PCI. Conversely, the mean hsCRP did not significantly differ at the follow-up points for the controls without ISR.

scholarly journals Ability of Procalcitonin and C-Reactive Protein for Discriminating between Bacterial and Enteroviral Meningitis in Children Using Decision Tree

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Dmitriy Babenko ◽  
Aliya Seidullayeva ◽  
Dinagul Bayesheva ◽  
Bayan Turdalina ◽  
Baurzhan Omarkulov ◽  
...  
Keyword(s):  
Decision Making ◽  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Decision Tree ◽  
Clinical Signs ◽  
Acute Bacterial Meningitis ◽  
C Reactive Protein ◽  
Public Health Burden ◽  
Reactive Protein ◽  
Enteroviral Meningitis

Bacterial meningitis (BM) is a public health burden in developing countries, including Central Asia. This disease is characterized by a high mortality rate and serious neurological complications. Delay with the start of adequate therapy is associated with an increase in mortality for patients with acute bacterial meningitis. Cerebrospinal fluid culture, as a gold standard in bacterial meningitis diagnosis, is time-consuming with modest sensitivity, and this is unsuitable for timely decision-making. It has been shown that bacterial meningitis differentiation from viral meningitis could be done through different parameters such as clinical signs and symptoms, laboratory values, such as PCR, including blood and cerebrospinal fluid (CSF) analysis. In this study, we proposed the method for distinguishing the bacterial form of meningitis from enteroviral one. The method is based on the machine learning process deriving making decision rules. The proposed fast-and-frugal trees (FFTree) decision tree approach showed an ability to determine procalcitonin and C-reactive protein (CRP) with cut-off values for distinguishing between bacterial and enteroviral meningitis (EVM) in children. Such a method demonstrated 100% sensitivity, 96% specificity, and 98% accuracy in the differentiation of all cases of bacterial meningitis in this study. These findings and proposed method may be useful for clinicians to facilitate the decision-making process and optimize the diagnostics of meningitis.

Usefulness of ischemia-modified albumin for assessment of the effects of small ventricular septal defects on the pulmonary vascular bed

2021 ◽  
pp. 1-6
Author(s):  
Pinar Dervisoglu ◽  
Taliha Oner
Keyword(s):  
Oxidative Stress ◽  
High Sensitivity ◽  
Control Group ◽  
Ventricular Septal Defects ◽  
C Reactive Protein ◽  
Ischemia Modified Albumin ◽  
Reactive Protein ◽  
Septal Defects ◽  
Z Scores ◽  
The Mean

Abstract Background: Pulmonary vascular damage may be associated with oxidative stress in congenital heart diseases. We investigated whether small ventricular septal defects have an effect on the pulmonary bed. Methods: This prospective cohort study included 100 patients with small ventricular septal defects and 75 healthy controls. Ischemia-modified albumin, high-sensitivity C-reactive protein, and various cardiovascular parameters were assessed in both groups. Results: The mean ischemia-modified albumin level was significantly higher in patients with small ventricular septal defects (0.62 ± 0.17 absorbance units) than in the control group (0.51 ± 0.09 absorbance units; p < 0.001). The mean high-sensitivity C-reactive protein level was significantly higher in the ventricular septal defects group (3.72 ± 1.57) than in the control group (2.45 ± 0.89; p < 0.001). The ischemia-modified albumin levels in patients with left ventricular internal diameter end diastole and end sistole and main pulmonary artery z-scores ≥ 2 were significantly higher than patients whose z-scores were <2. The ischemia-modified albumin and high-sensitivity C-reactive protein levels were positively correlated in the small ventricular septal defects group (rho = 0.742, p < 0.001). Receiver operating characteristic analyses showed that at the optimal cut-off value of ischemia-modified albumin for the prediction of pulmonary involvement was 0.55 absorbance units with a sensitivity of 60%, specificity of 62% (area under the curve = 0.690, p < 0.001). Conclusions: We demonstrated the presence of oxidative stress and higher ischemia-modified albumin levels in small ventricular septal defects, suggesting that ischemia-modified albumin might be a useful biomarker for evaluating the effects of small ventricular septal defects on the pulmonary bed.

scholarly journals Role of CSF C-Reactive Protein for the Differentiation of Bacterial Meningitis from Aseptic Meningitis in Children

2013 ◽  
Vol 36 (3) ◽  
pp. 126-132
Author(s):  
Rafeza Khanam ◽  
Mohammad Hanif ◽  
Md Mahbubul Hoque ◽  
Muhammad Tawfique ◽  
ASM Nawshad Uddin Ahmed
Keyword(s):  
Bacterial Meningitis ◽  
Aseptic Meningitis ◽  
Predictive Value ◽  
Acute Bacterial Meningitis ◽  
Control Group ◽  
C Reactive Protein ◽  
Predictive Values ◽  
Mortality And Morbidity ◽  
Reactive Protein ◽  
Csf Findings

Background: Acute bacterial meningitis (ABM) is one of the common causes of morbidity and mortality in children. Easy and early diagnostic tool is required for rapid detection of acute bacterial meningitis to reduce mortality and morbidity. Objectives: The study was conducted with the aim to identify the importance of cerebrospinal fluid C- reactive protein (CSF-CRP) to establish the diagnosis of ABM, and to measure the specificity, sensitivity, positive and negative predictive values of CSF-CRP in the diagnosis of Acute Bacterial Meningitis. Methods: This prospective study was carried out in Dhaka Shishu (Children) Hospital during the period of December 2004 to April 2005. Children admitted between age 0- 12 years with fever and convulsion were screened. Patients were divided into three groups on the basis of CSF findings- bacterial meningitis, aseptic meningitis and no meningitis control group. Only CSF culture proven cases were selected as ABM. CSF CRP was measured in all cases along with CSF cytology, biochemistry and culture-sensitivity. Complete were blood count, random blood sugar and other tests also done to treat all cases. All patients were treated adequately (if culture positive according to sensitivity) and were monitored as long as they stayed in hospital. Outcome was assessed clinically during discharge. Results: Twenty patients had acute bacterial meningitis, 15 aseptic meningitis, and 15 cases CSF findings normal and taken as control. CSF-CRP was positive (>6mg/ L) in 35% of the cases of ABM but it was found negative in all aseptic meningitis and in the control groups. Sensitivity of CSF -CRP was low (35%) but specificity was high (100%). The positive predictive value of CSF-CRP was 100% and negative predictive value 53.6%. Among the organisms isolated from acute bacterial meningitis, H. influenzae was the leading pathogen (40%) in infancy followed by S. pneumoniae (35%) and N. meningitidis (5%). Out come of the treatment of CSF-CRP positive ABM cases was found poor (p= 0.035). Conclusion: It is concluded from the study that significant(>6mg/L) level of CRP in CSF is highly specific for diagnosis of ABM and ruled out aseptic meningitis. But negative CSF-CRP could neither exclude pyogenic meningitis nor did it rule out aseptic meningitis. DOI: http://dx.doi.org/10.3329/bjch.v36i3.14275 BANGLADESH J CHILD HEALTH 2012; VOL 36 (3) : 126-132

scholarly journals Laboratory-Based Cytological and Biochemical Profile of Cerebrospinal Fluid for Children With Acute Bacterial Meningitis in Bangladesh

2021 ◽  
Vol 47 (2) ◽  
pp. 137-147
Author(s):  
Mohammad Zakerin Abedin ◽  
Laila Jarin ◽  
Donald James Gomes
Keyword(s):  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Protein Concentration ◽  
Acute Bacterial Meningitis ◽  
Biochemical Profile ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Cell Counts ◽  
Leukocyte Counts ◽  
Etiological Agents

An attempt was made to analyze the cerebrospinal fluid (CSF) for a cytological and biochemical profile to identify etiological agents from children with suspected acute bacterial meningitis. The cerebrospinal fluid (CSF) samples from 371 suspected meningitis cases were examined, and the highest bacterial meningitis was found in 52(14.0%) cases in this study. Among a total of 371 samples of CSF, 272(73.3%) were crystal clear, 52(14.0%) were moderately turbid, 47(12.7%) highly turbid. The total leukocyte cell count of the CSF was proportionate to the turbidity. In the case of crystal clear CSF’s, total leukocyte counts (TLC) were normally ranging from <5 to 45 per mm3 with predominant lymphocytes. Moderately turbid fluid showed 46 to 500 cells per mm3 and highly turbid fluid showed from 501 to more than 10,00 cells/mm3. In the latter cases, differential counts demonstrated polymorphonuclear predominance. In addition, about 100% (52 cases) of positive and 12.5% (40/319) of negative cases had CSF protein concentration >100 mg/dL. CSF protein concentration greater than 100 mg/dl and sugar level below 40 mg/dl were considered as suspected bacterial meningitis in this study. Surprisingly, the C-Reactive Protein (CRP) values were found to be >40 mg/dL in both culturally positive and negative cases. Most of meningitis positive cases showed increased total cell counts as well as protein concentration, and decreased serum sugar concentrations. J. Asiat. Soc. Bangladesh, Sci. 47(2): 137-147, December 2021

Prediction of lung cancer risk using circulating pro-surfactant protein B and high-sensitivity C-reactive protein among Egyptian workers in the rubber industry

2020 ◽  
Vol 36 (4) ◽  
pp. 237-249
Author(s):  
Basma Hussein Mourad
Keyword(s):  
Lung Cancer ◽  
Manufacturing Process ◽  
High Sensitivity ◽  
Surfactant Protein ◽  
Control Group ◽  
C Reactive Protein ◽  
Rubber Industry ◽  
Reactive Protein ◽  
Surfactant Protein B ◽  
Exposed Workers

Objective: For several decades, there has been increasing evidence for excess incidence of lung cancer among workers in the rubber industry. The purpose of this study was to assess the risk of lung cancer occurrence among Egyptian workers involved in the rubber industry using two circulating protein biomarkers. Methods: This study was performed in a rubber manufacturing factory in Shubra El-Kheima region in Greater Cairo, Egypt. Environmental assessment for the suspended particulate matter of size 10 µm (PM10) concentrations was done. Levels of plasma pro-surfactant protein B (pro-SFTPB) and serum high-sensitivity C-reactive protein (HsCRP) were measured among the studied population ( n = 155) who were divided into two groups. The first group included 75 workers exposed to rubber manufacturing process while the control group involved 80 administrative subjects. Results: The levels of PM10 neither exceeded the Egyptian nor the international permissible limits where the highest levels were observed in the mixing department. However, through medical history and clinical examination, it was observed that some general and respiratory manifestations were more prevalent among the exposed group when compared with their controls. Laboratory investigations revealed that the mean values of pro-SFTPB and HsCRP levels among exposed workers were significantly higher than those of the control group. These increased circulating proteins levels were strongly and positively correlated with each other and with the duration of employment of exposed workers. Conclusion: The study results support the conclusion that prolonged occupational exposure to rubber manufacturing process is associated with an elevated risk of lung cancer.

scholarly journals 1104 QUANTITATIVE MEASUREMENT OF C-REACTIVE PROTEIN (CRP) IN CEREBROSPINAL FLUID (CSF) OF PATIENTS WITH BACTERIAL MENINGITIS

1985 ◽  
Vol 19 (4) ◽  
pp. 294A-294A
Author(s):  
Barry M Gray ◽  
Don Simmons ◽  
Henry F Mason ◽  
Scott K Barnum ◽  
John E Volanakis
Keyword(s):  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Quantitative Measurement ◽  
C Reactive Protein ◽  
Reactive Protein
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