scholarly journals HIV-1 DRUG RESISTANCE ASSESSMENT IN PEDIATRIC PATIENTS

2009 ◽  
Vol 14 (2) ◽  
Author(s):  
Sandra R. R. Simonetti ◽  
Dirce B. De Lima ◽  
Hermann G. Schatzmayr ◽  
Bruno R. Simonetti ◽  
Denise C. N. Sztajnbok ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Pediatric Patients ◽  
Resistance Assessment ◽  
Hiv 1

The Frequency of HIV-1 Infection in Iranian Children and Determination of the Transmitted Drug Resistance in Treatment-Naïve Children

2020 ◽  
Vol 17 (6) ◽  
pp. 397-407
Author(s):  
Maryam Jarchi ◽  
Farah Bokharaei-Salim ◽  
Maryam Esghaei ◽  
Seyed Jalal Kiani ◽  
Fatemeh Jahanbakhsh ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Pediatric Patients ◽  
Phylogenetic Analyses ◽  
Rna Extraction ◽  
Transmitted Drug Resistance ◽  
The Arts ◽  
Treatment Naïve ◽  
Iranian Children ◽  
Hiv 1

Background: The advent of resistance-associated mutations in HIV-1 is a barrier to the success of the ARTs. Objective: In this study, the abundance of HIV-1 infection in Iranian children, and also detection of the TDR in naïve HIV-1 infected pediatric (under 12 years old) were evaluated. Materials: From June 2014 to January 2019, a total of 544 consecutive treatment-naïve HIV-1- infected individuals enrolled in this study. After RNA extraction, amplification, and sequencing of the HIV-1 pol gene, the DRM and phylogenetic analysis were successfully performed on the plasma specimens of the ART-naïve HIV-1-infected-children under 12 years old. The DRMs were recognized using the Stanford HIV Drug Resistance Database. Results: Out of the 544 evaluated treatment-naïve HIV-1-infected individuals, 15 (2.8%) cases were children under 12 years old. The phylogenetic analyses of the amplified region of pol gene indicated that all of the 15 HIV-1-infected pediatric patients were infected by CRF35_AD, and a total of 13.3% (2/15) of these children were infected with HIV-1 variants with SDRMs (one child harbored two related SDRMs [D67N, V179F], and another child had three related SDRMs [M184V, T215F, and K103N]), according to the last algorithm of the WHO. No PIs-related SDRMs were observed in HIV-1-infected children. Conclusion: The current study demonstrated that a total of 13.3% of treatment-naïve HIV-1-infected Iranian pediatrics (under 12 years old) were infected with HIV-1 variants with SDRMs. Therefore, it seems that screening to recognize resistance-associated mutations before the initiation of ARTs among Iranian children is essential for favorable medication efficacy and dependable prognosis.

scholarly journals Determination of Drug Resistance and Virus Typology in HIV-1-Positive Pediatric Patients in Istanbul, Turkey

Intervirology ◽  
2014 ◽  
Vol 57 (5) ◽  
pp. 297-299 ◽  
Author(s):  
Özlem Yoldaş ◽  
Ali Ağaçfidan ◽  
Nadine Lübke ◽  
Ayper Somer ◽  
Selda Hançerli ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Pediatric Patients ◽  
Hiv 1

scholarly journals ANTIRETROVIRAL THERAPY, VIRAL LOAD, AND T LYMPHOCYTES RATE IN HIV-1 INFECTED CHILDREN AFTER GENOTYPING DRUG RESISTANCE ASSESSMENT

2009 ◽  
Vol 14 (1) ◽  
Author(s):  
Sandra R. R. Simonetti ◽  
Dirce B. De Lima ◽  
Hermann G. Schatzmayr ◽  
Bruno R. Simonetti ◽  
Denise C. N. Sztajnbok ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Viral Load ◽  
Antiretroviral Therapy ◽  
T Lymphocytes ◽  
Resistance Assessment ◽  
Hiv 1

scholarly journals Targeting HIV-1 Protease Autoprocessing for High-throughput Drug Discovery and Drug Resistance Assessment

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Liangqun Huang ◽  
Linfeng Li ◽  
ChihFeng Tien ◽  
Daniel V. LaBarbera ◽  
Chaoping Chen
Keyword(s):  
Drug Resistance ◽  
Drug Discovery ◽  
High Throughput ◽  
Resistance Assessment ◽  
Hiv 1

scholarly journals Contribution of Gag and Protease to HIV-1 Phenotypic Drug Resistance in Pediatric Patients Failing Protease Inhibitor-Based Therapy

2016 ◽  
Vol 60 (4) ◽  
pp. 2248-2256 ◽  
Author(s):  
Jennifer Giandhari ◽  
Adriaan E. Basson ◽  
Katherine Sutherland ◽  
Chris M. Parry ◽  
Patricia A. Cane ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Amino Acid ◽  
Pediatric Patients ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Amino Acid Substitutions ◽  
Resistance Mutations ◽  
Phenotypic Resistance ◽  
Protease Resistance ◽  
Hiv 1

ABSTRACTProtease inhibitors (PIs) are used as a first-line regimen in HIV-1-infected children. Here we investigated the phenotypic consequences of amino acid changes in Gag and protease on lopinavir (LPV) and ritonavir (RTV) susceptibility among pediatric patients failing PI therapy. The Gag-protease from isolates from 20 HIV-1 subtype C-infected pediatric patients failing an LPV and/or RTV-based regimen was phenotyped using a nonreplicativein vitroassay. Changes in sensitivity to LPV and RTV relative to that of the matched baseline (pretherapy) sample were calculated. Gag and protease amino acid substitutions associated with PI failure were created in a reference clone by site-directed mutagenesis and assessed. Predicted phenotypes were determined using the Stanford drug resistance algorithm. Phenotypic resistance or reduced susceptibility to RTV and/or LPV was observed in isolates from 10 (50%) patients, all of whom had been treated with RTV. In most cases, this was associated with protease resistance mutations, but substitutions at Gag cleavage and noncleavage sites were also detected. Gag amino acid substitutions were also found in isolates from three patients with reduced drug susceptibilities who had wild-type protease. Site-directed mutagenesis confirmed that some amino acid changes in Gag contributed to PI resistance but only in the presence of major protease resistance-associated substitutions. The isolates from all patients who received LPV exclusively were phenotypically susceptible. Baseline isolates from the 20 patients showed a large (47-fold) range in the 50% effective concentration of LPV, which accounted for most of the discordance seen between the experimentally determined and the predicted phenotypes. Overall, the inclusion of thegaggene and the use of matched baseline samples provided a more comprehensive assessment of the effect of PI-induced amino acid changes on PI resistance. The lack of phenotypic resistance to LPV supports the continued use of this drug in pediatric patients.

Drug resistance among patients with HIV-1

2013 ◽  
Vol 4 (5) ◽  
pp. 317-323
Author(s):  
Miłosz Parczewski
Keyword(s):  
Drug Resistance ◽  
Hiv 1
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