scholarly journals Mechanism of the trade-off between biological nitrogen fixation and phosphorus acquisition strategies of herbaceous legumes under nitrogen and phosphorus addition

2021 ◽  
Vol 45 (3) ◽  
pp. 286-297
Author(s):  
Qiang LI ◽  
Ying-Xin HUANG ◽  
Dao-Wei ZHOU ◽  
Shan CONG ◽  
Keyword(s):  
Nitrogen Fixation ◽  
Biological Nitrogen Fixation ◽  
Phosphorus Acquisition ◽  
Nitrogen And Phosphorus ◽  
Phosphorus Addition ◽  
Trade Off ◽  
Herbaceous Legumes ◽  
Acquisition Strategies ◽  
Biological Nitrogen

Influence of P Fertilization on Biological Nitrogen Fixation in Herbaceous Legumes Grown in Acid Savannah Soils from the Tabasco State, Mexico

2008 ◽  
Vol 31 (3) ◽  
pp. 25-42 ◽  
Author(s):  
J. A. Vera-Nunez ◽  
J. P. Infante-Santiago ◽  
V. Velasco Velasco ◽  
S. Salgado Garcia ◽  
D. J. Palma-Lopez ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Biological Nitrogen Fixation ◽  
P Fertilization ◽  
Herbaceous Legumes ◽  
Biological Nitrogen

scholarly journals Arbuscular Mycorrhiza Stimulates Biological Nitrogen Fixation in Two Medicago spp. through Improved Phosphorus Acquisition

2017 ◽  
Vol 8 ◽  
Author(s):  
David Püschel ◽  
Martina Janoušková ◽  
Alena Voříšková ◽  
Hana Gryndlerová ◽  
Miroslav Vosátka ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Arbuscular Mycorrhiza ◽  
Biological Nitrogen Fixation ◽  
Phosphorus Acquisition ◽  
Biological Nitrogen

Biological nitrogen fixation and nitrogen and phosphorus budgets in farmer-managed intercrops of maize–pigeonpea in semi-arid southern and eastern Africa

2007 ◽  
Vol 295 (1-2) ◽  
pp. 127-136 ◽  
Author(s):  
Joseph J. Adu-Gyamfi ◽  
Fidelis A. Myaka ◽  
Webster D. Sakala ◽  
Rie Odgaard ◽  
Jens M. Vesterager ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Biological Nitrogen Fixation ◽  
Eastern Africa ◽  
Nitrogen And Phosphorus ◽  
Phosphorus Budgets ◽  
Biological Nitrogen ◽  
Semi Arid

scholarly journals Functional analysis of multiple nifB genes of Paenibacillus strains in synthesis of Mo-, Fe- and V-nitrogenases

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Qin Li ◽  
Haowei Zhang ◽  
Liqun Zhang ◽  
Sanfeng Chen
Keyword(s):  
Functional Analysis ◽  
Nitrogen Fixation ◽  
Gene Cluster ◽  
Biological Nitrogen Fixation ◽  
Deletion Analysis ◽  
Phylogeny Analysis ◽  
Biological Nitrogen

Abstract Background Biological nitrogen fixation is catalyzed by Mo-, V- and Fe-nitrogenases that are encoded by nif, vnf and anf genes, respectively. NifB is the key protein in synthesis of the cofactors of all nitrogenases. Most diazotrophic Paenibacillus strains have only one nifB gene located in a compact nif gene cluster (nifBHDKENX(orf1)hesAnifV). But some Paenibacillus strains have multiple nifB genes and their functions are not known. Results A total of 138 nifB genes are found in the 116 diazotrophic Paenibacillus strains. Phylogeny analysis shows that these nifB genes fall into 4 classes: nifBI class including the genes (named as nifB1 genes) that are the first gene within the compact nif gene cluster, nifBII class including the genes (named as nifB2 genes) that are adjacent to anf or vnf genes, nifBIII class whose members are designated as nifB3 genes and nifBIV class whose members are named as nifB4 genes are scattered on genomes. Functional analysis by complementation of the ∆nifB mutant of P. polymyxa which has only one nifB gene has shown that both nifB1 and nifB2 are active in synthesis of Mo-nitrogenase, while nifB3 and nifB4 genes are not. Deletion analysis also has revealed that nifB1 of Paenibacillus sabinae T27 is involved in synthesis of Mo-nitrogenase, while nifB3 and nifB4 genes are not. Complementation of the P. polymyxa ∆nifBHDK mutant with the four reconstituted operons: nifB1anfHDGK, nifB2anfHDGK, nifB1vnfHDGK and nifB2vnfHDGK, has shown both that nifB1 and nifB2 were able to support synthesis of Fe- or V-nitrogenases. Transcriptional results obtained in the original Paenibacillus strains are consistent with the complementation results. Conclusions The multiple nifB genes of the diazotrophic Paenibacillus strains are divided into 4 classes. The nifB1 located in a compact nif gene cluster (nifBHDKENX(orf1)hesAnifV) and the nifB2 genes being adjacent to nif or anf or vnf genes are active in synthesis of Mo-, Fe and V-nitrogenases, but nifB3 and nifB4 are not. The reconstituted anf system comprising 8 genes (nifBanfHDGK and nifXhesAnifV) and vnf system comprising 10 genes (nifBvnfHDGKEN and nifXhesAnifV) support synthesis of Fe-nitrogenase and V-nitrogenase in Paenibacillus background, respectively.

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